不同海拔和光照对黄芩中7种黄酮类有效成分含量的影响
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摘要
目的分析不同海拔、光照因素对黄芩中7种黄酮类有效成分量的影响,为黄芩种植最佳生长条件的选择提供理论依据。方法采用HPLC法同时测定人工种植的24批黄芩样品中野黄芩苷、黄芩苷、汉黄芩苷、芹菜素、黄芩素、汉黄芩素、千层纸素A7种成分的含量,并采用方差分析法进行不同海拔、光照环境(阴坡、阳坡)与各成分量的相关性分析。结果同一生长年限,在海拔(550±30)、(650±30)m 2个范围,对黄芩中7种成分的量进行比较,其中黄芩苷、汉黄芩苷有极显著性差异(P<0.01),野黄芩苷、黄芩素、千层纸素A、汉黄芩素、芹菜素有显著性差异(P<0.05);阴坡和阳坡2种不同的光照条件下,黄芩中7种成分的量相比,无显著性差异(P>0.05)。结论在同一生长年限,随海拔的升高,黄芩中黄酮类成分的量都呈现明显的上升趋势,有显著性差异。而光照(阴坡、阳坡)不同时,黄芩中黄酮类成分的量无显著性差异,但除汉黄芩苷、黄芩素外,其他黄酮类成分含量的均数阳坡高于阴坡。黄芩人工种植宜选择阳光直接照射的平地或阳坡,同时及海拔偏高有利于提高黄芩中黄酮类化学成分总量的积累。
Objective Effects of different elevations and illumination on the seven effective components of flavonoids in Scutellaria baicalensis were analyzed, providing a theoretical basis for selecting the best growing conditions. Methods The content of cutellarin,baicalin, wogonoside, apigenin, baicalein, wogonin, and oroxylin A were determined by HPLC method in 24 artificially planted samples.The correlation between different elevations, different lighting conditions(shady slope, sunny slope) and different components were analyzed by using variance analysis method. Results Compared with the same growth period, the amount of seven components in S.baicalensis was compared at elevation(550 ± 30) and(650 ± 30) m, and there was a significant difference between baicalins and wogonoside(P < 0.01). There have significant differences in the content of cutellarin, apigenin, baicalein, wogonin, oroxylin A, and apigenin(P < 0.05); There was no significant difference in the amount of seven components in S. baicalensis under different illumination conditions(P > 0.05). Conclusion In the same growing age, the amount of flavonoids in S. baicalensis showed an obvious upward trend with the increase of altitude, and there were significant differences. There was no significant difference in the content of flavonoids in S. baicalensis, but the average sunny slope of various components of other flavonoids was higher than the shady slope except for wogonoside and baicalein. Therefore, it is advisable to choose the plain or sunny slope directly illuminated by sunlight in the artificial cultivation of S. baicalensis, and the high altitude is conducive to increasing the accumulation of total flavonoids in S. baicalensis.
Objective Effects of different elevations and illumination on the seven effective components of flavonoids in Scutellaria baicalensis were analyzed, providing a theoretical basis for selecting the best growing conditions. Methods The content of cutellarin,baicalin, wogonoside, apigenin, baicalein, wogonin, and oroxylin A were determined by HPLC method in 24 artificially planted samples.The correlation between different elevations, different lighting conditions(shady slope, sunny slope) and different components were analyzed by using variance analysis method. Results Compared with the same growth period, the amount of seven components in S.baicalensis was compared at elevation(550 ± 30) and(650 ± 30) m, and there was a significant difference between baicalins and wogonoside(P < 0.01). There have significant differences in the content of cutellarin, apigenin, baicalein, wogonin, oroxylin A, and apigenin(P < 0.05); There was no significant difference in the amount of seven components in S. baicalensis under different illumination conditions(P > 0.05). Conclusion In the same growing age, the amount of flavonoids in S. baicalensis showed an obvious upward trend with the increase of altitude, and there were significant differences. There was no significant difference in the content of flavonoids in S. baicalensis, but the average sunny slope of various components of other flavonoids was higher than the shady slope except for wogonoside and baicalein. Therefore, it is advisable to choose the plain or sunny slope directly illuminated by sunlight in the artificial cultivation of S. baicalensis, and the high altitude is conducive to increasing the accumulation of total flavonoids in S. baicalensis.
引文
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[11]郭玲玲,刘毅,禄梦杰,等.HPLC法同时测定陕西产不同生长年限野生和栽培黄芩中9种化学成分的含量[J].中草药,2018,49(4):935-940.
[12]李恩泽,刘月芬,刘玉君,等.不同产地黄芩中6种化学成分含量测定[J].中国医院药学杂志,2018,38(9):946-948.
[13]党珍,张艳萍,王西芳.子洲黄芩中黄酮成分的含量测定及指纹图谱研究[J].中国现代中药,2017,19(2):200-204.
[14]冯学峰,胡世林,郭宝林,等.黄芩种群遗传多样性研究[J].世界科学技术,2002,4(4):38-43.
[15]刘岩,李连泰,计小清,等.土壤中无机元素对不同产地黄芩中无机元素和黄芩苷量的影响[J].中草药,2017,48(6):1225-1228.
[2]金敏.黄芩中黄酮类化学成分研究进展[J].中国民族医药杂志,2008,9(9):55-56.
[3]韩贞爱,汪娜,薛迪,等.黄芩有效成分治疗心脑血管疾病药理作用的研究进展[J].中国药房,2015,26(21):3015-3017.
[4]Horvath C R,Martos P A,Saxena P K.Identification and quantifi-cation of eight flavones in root and shoot tissues of the medicinal plant Huang-qin(Scutellaria baicalensis Georgi)using high-performance liquid chromatography with diode array massspectrometric detection[J].JChromatography A,2005,1062:199-207.
[5]郑勇凤,王佳婧,傅超美,等.黄芩的化学成分与药理作用研究进展[J].中成药,2016,38(1):141-147.
[6]张瑜,武斌,许建卫.黄芩药理作用的研究进展[J].医学综述,2013,19(6):1091-1093.
[7]申云富,范小青.汉黄芩苷的药理活性研究进展[J].上海中医药大学学报,2016,30(4):98-101.
[8]姜茗宸,徐秋月.黄芩素抗病毒作用研究[J].杏林中医药,2016,36(7):753-756.
[9]赵田禾,张莹莹,吴洋洋,等.汉黄芩素及其衍生物的抗肿瘤作用及其分子机制研究进展[J].中国新药杂志,2016,25(7):760-766.
[10]刘金欣,孟繁蕴,张胜海,等.UPLC同时测定黄芩中黄芩苷、黄芩素、汉黄芩苷、汉黄芩素、千层纸素A[J].中草药,2014,45(10):1477-1480.
[11]郭玲玲,刘毅,禄梦杰,等.HPLC法同时测定陕西产不同生长年限野生和栽培黄芩中9种化学成分的含量[J].中草药,2018,49(4):935-940.
[12]李恩泽,刘月芬,刘玉君,等.不同产地黄芩中6种化学成分含量测定[J].中国医院药学杂志,2018,38(9):946-948.
[13]党珍,张艳萍,王西芳.子洲黄芩中黄酮成分的含量测定及指纹图谱研究[J].中国现代中药,2017,19(2):200-204.
[14]冯学峰,胡世林,郭宝林,等.黄芩种群遗传多样性研究[J].世界科学技术,2002,4(4):38-43.
[15]刘岩,李连泰,计小清,等.土壤中无机元素对不同产地黄芩中无机元素和黄芩苷量的影响[J].中草药,2017,48(6):1225-1228.