马齿苋中抑制马拉色菌活性成分的提取工艺研究
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摘要
采用超声波辅助提取法提取马齿苋中抑制糠秕马拉色菌的活性成分,通过纸片法测定其抑菌效果,并以抑菌效果为指标,采用单因素试验确定较佳提取工艺。结果表明,当提取温度为60℃,提取时间为50 min,料液比为1∶25(g∶mL),超声功率为210 W时,所得提取物的抑菌效果较佳,抑菌圈直径为(46.68±1.21)mm。通过理化反应对马齿苋提取物中的成分进行初步检识,结果表明马齿苋提取物中存在生物碱类、皂苷类、蛋白质多肽或氨基酸和糖类,可能含有鞣质及酚类、黄酮类和三萜类,不存在有机酸类。
The active ingredients of anti-Malassezia furfur were extracted by ultrasonic assisted extraction process from Portulaca oleracea L.. The bacteriostatic effect was determined by the paper method,and the single factor test was used to explore the better extraction process by using the bacteriostatic effect as the index.Results show that when extraction temperature is 60 ℃,extraction time is 50 min,ratio of material to liquid is 1∶ 25( g∶ mL),and the ultrasonic power is 210 W,the extract has better bacteriostatic effect and the diameter of inhibition zone is( 46. 68 ± 1. 21) mm. Through physical and chemical reaction,the ingredients of purslane extract were preliminarily detected. Results show that there are alkaloids,saponins,proteins,peptides or amino acids and sugars in the purslane extract,and it may also contain tannins and phenols,flavonoids and triterpenes,while there are no organic acids.
The active ingredients of anti-Malassezia furfur were extracted by ultrasonic assisted extraction process from Portulaca oleracea L.. The bacteriostatic effect was determined by the paper method,and the single factor test was used to explore the better extraction process by using the bacteriostatic effect as the index.Results show that when extraction temperature is 60 ℃,extraction time is 50 min,ratio of material to liquid is 1∶ 25( g∶ mL),and the ultrasonic power is 210 W,the extract has better bacteriostatic effect and the diameter of inhibition zone is( 46. 68 ± 1. 21) mm. Through physical and chemical reaction,the ingredients of purslane extract were preliminarily detected. Results show that there are alkaloids,saponins,proteins,peptides or amino acids and sugars in the purslane extract,and it may also contain tannins and phenols,flavonoids and triterpenes,while there are no organic acids.
引文
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[3]胡梅,李海军,彭巍.植物去屑止痒剂的研究现状与展望[J].日用化学品科学,2011,34(2):13-15.
[4]宋杰,赵文忠,洪盛杰,等.常用去屑剂的安全研究进展[J].日用化学品科学,2012,35(7):20-23.
[5]中华人民共和国卫生部药典委员会.中华人民共和国药典:一部[M].北京:化学工业出版社,2010:324-325.
[6]战兴花,田英林.马齿苋的药用价值[J].中国林副特产,2000(1):55.
[7]王莉,刘志勇,鲁建江,等.微波技术辅助测定马齿苋中总黄酮和多糖的含量[J].食品工业科技,2002,23(5):70-73.
[8]刘志勇,王莉,鲁建江,等.马齿苋多糖的微波提取及含量测定[J].中国药师杂志,2002(11):667-669.
[9]Parry O,Okwuasaba F,Eijike C.Effect of an aqueous extract of Portulaca olearcea leaves on smooth muscle and rat blood pressure[J].Journal of Ethnopharmacology,1988,22:33-44.
[10]Shen H,Tang G,Zeng G,et al.Purification and characterization of an antitumor polysaccharide from Portulaca oleracea L.[J].Carbohydrate Polymers,2013,93:395-400.
[11]Parry O,Okwausaba F,Ejike C.Preliminary investigation into the muscle relaxant actions of an aqueous extract of P.oleracea applied topically[J].J.Ethnopharmaeology,1987,21:99-106.
[12]库尔班·吾斯曼,穆赫塔尔·伊米尔艾山,帕提古丽·伊米尔艾山.马齿苋子中多糖的提取分离及抑菌活性研究[J].食品研究与开发杂志,2009(7):28-30.
[13]谷春梅,潘姝.马齿苋黄酮提取工艺及其分离纯化和抑菌作用的研究[J].食品科技,2015(12):185-189.
[14]张海艳,郑玲.马齿苋多糖的提取及体外抗菌活性[J].江苏农业科学,2011,39(5):413-415.
[15]宋杰,董银卯,孟宏,等.复方植物抑菌活性提取工艺的优化[J].中国化妆品(行业),2013(3):76-81.
[16]Palanivel S,Johnson P,Raji V,et al.Anti-acne,anti-dandruff and anti-breast cancer efficacy of green synthesised silver nanoparticles using Coriandrum sativum leaf extract[J].J.Photochem.Photobiol.B,2016,163:69-76.
[17]Onlom C,Khanthawong S,Waranuch N.In vitro anti-Malassezia activity and potential use in anti-dandruff formulation of Asparagus racemosus[J].International Journal of Cosmetic Science,2014,36(1):74-78.
[18]刘湘,汪秋安.天然产物化学[M].北京:化学工业出版社,2005.
[19]潘姝.马齿苋中多糖和黄酮的分离纯化及其抑菌作用的研究[D].长春:吉林农业大学,2015.