二氯喹啉酸对稗草生长发育及生长素含量的影响
|
摘要
通过外施二氯喹啉酸、生长素处理,研究二氯喹啉酸对稗草Echinochloacrusgalli生长特性、蛋白浓度、过氧化物酶及超氧化物歧化酶活性、生长素含量的影响。结果表明,800 g·hm~(-2)二氯喹啉酸处理24 h未对S型稗草的生长产生明显抑制,而二氯喹啉酸单独处理及外施IAA的二氯喹啉酸处理均能显著增加R型稗草鲜重。在不施药、二氯喹啉酸单独处理、二氯喹啉酸与IAA共同处理的条件下,R型稗草比S型稗草的蛋白浓度分别提高23.5%、29.8%和64.0%,R型稗草表现出较高的抗逆境耐受能力。经二氯喹啉酸处理后,两种生物型稗草SOD活性上升,POD活性下降。施外源IAA能提高S型稗草POD活性,而对R型稗草SOD、POD活性影响不显著。提高生长素含量可显著促进R型稗草的生长。
The effects of quinclorac on growth characteristics, protein concentration, peroxidase activity, superoxide dismutase activity and auxin content in Echinochloa crusgalli were studied by applying quinclorac and auxin. The results showed that the treatment of 800 g·hm~(-2) quinclorac for24 h did not significantly inhibit the growth of S-type barnyardgrass. However, the average fresh weight of R-barnyardgrass was significantly increased by quinclorac treatment alone and the treatment of quinclorac with IAA. Under the conditions of no medicine application, quinclorac treatment alone and the treatment of quinclorac with IAA, the protein concentration of R-type barnyardgrass increased by 23.5%, 29.8% and 64.0% than that of S-type barnyardgrass respectively.R-type barnyardgrass showed higher tolerance. After treated with quinclorac, the SOD activity of two biotypes of barnyardgrass increased and the POD activity decreased. Exogenous IAA can enhance the POD activity of S-type barnyardgrass; while there was no significant effect on SOD, POD activity of R-type barnyardgrass. The increase of auxin content promoted the growth of R-type barnyardgrass.
The effects of quinclorac on growth characteristics, protein concentration, peroxidase activity, superoxide dismutase activity and auxin content in Echinochloa crusgalli were studied by applying quinclorac and auxin. The results showed that the treatment of 800 g·hm~(-2) quinclorac for24 h did not significantly inhibit the growth of S-type barnyardgrass. However, the average fresh weight of R-barnyardgrass was significantly increased by quinclorac treatment alone and the treatment of quinclorac with IAA. Under the conditions of no medicine application, quinclorac treatment alone and the treatment of quinclorac with IAA, the protein concentration of R-type barnyardgrass increased by 23.5%, 29.8% and 64.0% than that of S-type barnyardgrass respectively.R-type barnyardgrass showed higher tolerance. After treated with quinclorac, the SOD activity of two biotypes of barnyardgrass increased and the POD activity decreased. Exogenous IAA can enhance the POD activity of S-type barnyardgrass; while there was no significant effect on SOD, POD activity of R-type barnyardgrass. The increase of auxin content promoted the growth of R-type barnyardgrass.
引文
[1]Yang X,Zhang Z C,Gu T,Dong M C,Peng Q,Bai L Y,Li Y F.Quantitative proteomics reveals ecological fitness cost of multi herbicide resistant barnyardgrass(Echinochloa crusgalli L.)[J].Journal of Proteomics,2017,150:160-169.
[2]Heap I.International survey of herbicide resistant weeds.http://www.weedscience.com,2018.
[3]Sunohara Y,Matsumoto H.Oxidative injury induced by the herbicide quinclorac on Echinochloa oryzicola Vasing.and the involvement of antioxidative ability in its highly selective action in grass species[J].Plant Science,2004,167(3):597-606.
[4]马国兰,柏连阳,刘都才,刘雪源,余柳青.我国长江中下游稻区稗草对二氯喹啉酸的抗药性研究[J].中国水稻科学,2013,27(2):184-190.
[5]Salehin M,Bagchi R,Estelle M.SCFTIR1/AFB-based auxin perception:mechanism and role in plant growth and development[J].Plant Cell,2015,27(1):9-19.
[6]Li G,Xu M F,Chen L P,Cai L M,Bai L Y,Wu C X.A novel Ec GH3 gene with a different expression pattern in quinclorac-resistant and susceptible barnyardgrass(Echinochloa crusgalli)[J].Plant Gene,2016,5:65-70.
[7]Xu J Y,Lv B,Wang Q,Li J,Dong L Y.A resistance mechanism dependent upon the inhibition of ethylene biosynthesis[J].Pest Management Science,2013,69(12):1407-1414.
[8]Fipke M V,Vidal R A.Integrative theory of the mode of action of quinclorac:literature review[J].Planta Daninha,2016,34:393-402.
[9]Gao Y,Li J,Pan X K,Liu D R,Napier R,Dong L Y.Quinclorac resistance induced by the suppression of the expression of1-aminocyclopropane-1-carboxylic acid(ACC)synthase and ACC oxidase genes in Echinochloa crusgalli var.zelayensis[J].Pesticide Biochemistry and Physiology,2018,146:25-32.
[10]Grossmann K,Kwiatkowski J.Selective induction of ethylene and cyanide biosynthesis appears to be involved in the selectivity of the herbicide quinclorac between rice and barnyardgrass[J].Journal of Plant Physiology,1993,142(4):457-466.
[11]Grossmann K,Kwiatkowski J.The mechanism of quinclorac selectivity in grasses[J].Pesticide Biochemistry and Physiology,2000,66(2):83-91.
[12]Grossmann K.Auxin herbicides:current status of mechanism and mode of action[J].Pest Management Science,2010,66:113-120.
[13]Gao Y,Pan L,Sun Y,Zhang T,Dong L Y,Li J.Resistance to quinclorac caused by the enhanced ability to detoxify cyanide and its molecular mechanism in Echinochloa crusgalli var.zelayensis[J].Pesticide Biochemistry and Physiology,2017,143:231-238.
[14]Peng Q,Wang H Q,Tong J H,Kabir M H,Huang Z G,Xiao L T.Effects of indole-3-acetic acid and auxin transport inhibitor on auxin distribution and development of at pegging stage[J].Scientia Horticulturae,2013,162:76-81.
[15]Yu Q,Powles S.Metabolism-based herbicide resistance and cross-resistance in crop weeds:A threat to herbicide sustainability and global crop production[J].Plant Physiology,2014,166:1106-1118.
[16]李晶新.二氯喹啉酸对烤烟生理特性影响的研究[D].河南:河南农业大学硕士学位论文,2010.
[17]Durian G,Rahikainen M,Alegre S.Protein phosphatase 2A in the regulatory network underlying biotic stress resistance in plants[J].Frontiers in Plant Science,2016,6(7):1-17.
[18]陈晶.生长素类物质对镉胁迫下玉米幼苗生长及生理特性的影响[D].重庆:西南大学硕士学位论文,2016.
[19]李永丰,张自常,杨霞,董明超,张彬,韩建勇.稻田稗属杂草对芳氧苯氧丙酸酯类除草剂的差异敏感性及其机理[J].江苏农业学报,2015,31(3):543-551.
[20]徐江艳.稻田西来稗(Echinochloa crusgalli var.zelayemis)对二氯喹啉酸的抗药性及其机理研究[D].南京:南京农业大学硕士学位论文,2013.
[21]陈晶,庞思琪,赵秀兰.外源生长素对镉胁迫下玉米幼苗生长及抗氧化系统的影响[J].植物生理学报,2016,52(8):1191-1198.
[22]李静,崔继哲,弭晓菊.生长素与植物逆境胁迫关系的研究进展[J].生物技术通报,2012(6):13-17.
[2]Heap I.International survey of herbicide resistant weeds.http://www.weedscience.com,2018.
[3]Sunohara Y,Matsumoto H.Oxidative injury induced by the herbicide quinclorac on Echinochloa oryzicola Vasing.and the involvement of antioxidative ability in its highly selective action in grass species[J].Plant Science,2004,167(3):597-606.
[4]马国兰,柏连阳,刘都才,刘雪源,余柳青.我国长江中下游稻区稗草对二氯喹啉酸的抗药性研究[J].中国水稻科学,2013,27(2):184-190.
[5]Salehin M,Bagchi R,Estelle M.SCFTIR1/AFB-based auxin perception:mechanism and role in plant growth and development[J].Plant Cell,2015,27(1):9-19.
[6]Li G,Xu M F,Chen L P,Cai L M,Bai L Y,Wu C X.A novel Ec GH3 gene with a different expression pattern in quinclorac-resistant and susceptible barnyardgrass(Echinochloa crusgalli)[J].Plant Gene,2016,5:65-70.
[7]Xu J Y,Lv B,Wang Q,Li J,Dong L Y.A resistance mechanism dependent upon the inhibition of ethylene biosynthesis[J].Pest Management Science,2013,69(12):1407-1414.
[8]Fipke M V,Vidal R A.Integrative theory of the mode of action of quinclorac:literature review[J].Planta Daninha,2016,34:393-402.
[9]Gao Y,Li J,Pan X K,Liu D R,Napier R,Dong L Y.Quinclorac resistance induced by the suppression of the expression of1-aminocyclopropane-1-carboxylic acid(ACC)synthase and ACC oxidase genes in Echinochloa crusgalli var.zelayensis[J].Pesticide Biochemistry and Physiology,2018,146:25-32.
[10]Grossmann K,Kwiatkowski J.Selective induction of ethylene and cyanide biosynthesis appears to be involved in the selectivity of the herbicide quinclorac between rice and barnyardgrass[J].Journal of Plant Physiology,1993,142(4):457-466.
[11]Grossmann K,Kwiatkowski J.The mechanism of quinclorac selectivity in grasses[J].Pesticide Biochemistry and Physiology,2000,66(2):83-91.
[12]Grossmann K.Auxin herbicides:current status of mechanism and mode of action[J].Pest Management Science,2010,66:113-120.
[13]Gao Y,Pan L,Sun Y,Zhang T,Dong L Y,Li J.Resistance to quinclorac caused by the enhanced ability to detoxify cyanide and its molecular mechanism in Echinochloa crusgalli var.zelayensis[J].Pesticide Biochemistry and Physiology,2017,143:231-238.
[14]Peng Q,Wang H Q,Tong J H,Kabir M H,Huang Z G,Xiao L T.Effects of indole-3-acetic acid and auxin transport inhibitor on auxin distribution and development of at pegging stage[J].Scientia Horticulturae,2013,162:76-81.
[15]Yu Q,Powles S.Metabolism-based herbicide resistance and cross-resistance in crop weeds:A threat to herbicide sustainability and global crop production[J].Plant Physiology,2014,166:1106-1118.
[16]李晶新.二氯喹啉酸对烤烟生理特性影响的研究[D].河南:河南农业大学硕士学位论文,2010.
[17]Durian G,Rahikainen M,Alegre S.Protein phosphatase 2A in the regulatory network underlying biotic stress resistance in plants[J].Frontiers in Plant Science,2016,6(7):1-17.
[18]陈晶.生长素类物质对镉胁迫下玉米幼苗生长及生理特性的影响[D].重庆:西南大学硕士学位论文,2016.
[19]李永丰,张自常,杨霞,董明超,张彬,韩建勇.稻田稗属杂草对芳氧苯氧丙酸酯类除草剂的差异敏感性及其机理[J].江苏农业学报,2015,31(3):543-551.
[20]徐江艳.稻田西来稗(Echinochloa crusgalli var.zelayemis)对二氯喹啉酸的抗药性及其机理研究[D].南京:南京农业大学硕士学位论文,2013.
[21]陈晶,庞思琪,赵秀兰.外源生长素对镉胁迫下玉米幼苗生长及抗氧化系统的影响[J].植物生理学报,2016,52(8):1191-1198.
[22]李静,崔继哲,弭晓菊.生长素与植物逆境胁迫关系的研究进展[J].生物技术通报,2012(6):13-17.