猪精液超低温冷冻保存研究进展
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摘要
猪人工授精(AI)技术已经广泛应用于畜牧业生产,但精液来源基本是17℃保存的新鲜猪精液,不利于精液长期保存和猪种资源保存。超低温冷冻保存技术是猪精液长期保存最有效的方法,冷冻精液可以实现不同地区、不同国家优良种猪精液间的交流,从而大大提高猪种资源的利用率,但冷冻解冻后的精液质量较差,受胎率、窝产仔数远低于鲜精,极大地限制了猪精液冷冻保存技术的广泛应用。本文综述了猪精液冷冻保存研究概况、猪精液冷冻损伤机理和猪精液冷冻保存影响因素,以期为猪精液超低温冷冻保存研究提供新的思路和方法。
Pig artificial insemination(AI) technology has been widely used in animal husbandry, but the source of boar semen is basically the fresh pig semen stored at 17℃, which is not conducive to long-term preservation of boar semen and preservation of pig resources. Cryopreservation is the most effective method for long-term preservation of boar semen. Frozen boar semen in different countries and regions can achieve excellent boar semen exchanges, thus greatly improving the utilization rate of pig resources. But the poor quality of frozen thawed boar semen, pregnancy rate, litter size is far lower than that of fresh sperm, which greatly limits the wide application of frozen boar semen preservation technology. Here, we review the research progress on cryopreservation of pig semen, cryopreservation mechanism of boar semen and factors affecting cryopreservation of boar semen, which will help to provide new ideas and research methods for cryopreservation of boar semen.
Pig artificial insemination(AI) technology has been widely used in animal husbandry, but the source of boar semen is basically the fresh pig semen stored at 17℃, which is not conducive to long-term preservation of boar semen and preservation of pig resources. Cryopreservation is the most effective method for long-term preservation of boar semen. Frozen boar semen in different countries and regions can achieve excellent boar semen exchanges, thus greatly improving the utilization rate of pig resources. But the poor quality of frozen thawed boar semen, pregnancy rate, litter size is far lower than that of fresh sperm, which greatly limits the wide application of frozen boar semen preservation technology. Here, we review the research progress on cryopreservation of pig semen, cryopreservation mechanism of boar semen and factors affecting cryopreservation of boar semen, which will help to provide new ideas and research methods for cryopreservation of boar semen.
引文
[1]Polge C.Artificial insemination in pigs[J].Vet Rec,1956,68:62-76.
[2]Polge C,Salamon S,Wilmut I.Fertilizing capacity of frozen boar semen following surgical insemination[J].Vet Rec,1970,87:424-429.
[3]Pursel V G,Johnson L A.Freezing of boar spermatozoa:fertilizing capacity with concentrated semen and a new thawing procedure[J].J Anim Sci,1975,40(1):99-102.
[4]Westendorf P,Richter L,Treu H.Deep freezing of boar sperma.Laboratory and insemination results using the Hülsenberger paillete method[J].Deut Tierarztl Woch,1975,82(7):261.
[5]Almlid T,Johnson L A.Effects of glycerol concentration,equilibration time and temperature of glycerol addition on postthaw viability of boar spermatozoa frozen in straws 1[J].JAnim Sci,1988,66(11):2899-2905.
[6]Fiser P S,Fairfull R W.Combined effect of glycerol concentration and cooling velocity on motility and acrosomal integrity of boar spermatozoa frozen in 0.5 ml straws[J].Mol Reprod Dev,1990,25(2):123-129.
[7]Córdova A,Ducolomb Y,Jiménez I,et al.In vitro fertilizing capacity of frozen-thawed boar semen[J].Theriogenology,1997,47(7):1309-1317.
[8]Petrounkina A M,Harrison R A,Petzoldt R,et al.Cyclical changes in sperm volume during in vitro incubation under capacitating conditions:a novel boar semen characteristic[J].J Reprod Fert,2000,118(2):283.
[9]Chanapiwat P,Kaeoket K,Tummaruk P.Cryopreservation of boar semen by egg yolk-based extenders containing lactose or fructose is better than sorbitol[J].J Vet Med Sci,2012,74(3):351-354.
[10]Okazaki T,Shimada M.New strategies of boar sperm cryopreservation:development of novel freezing and thawing methods with a focus on the roles of seminal plasma[J].Anim Sci J,2012,83(9):623-629.
[11]Tomás C,Gómez‐Fernández J,Gómez‐Izquierdo E,et al.Addition of cholesterol‐loaded cyclodextrins to the thawing extender:effects on boar sperm quality[J].Reprod Domest Anim,2014,49(3):427-432.
[12]Silva C G,Cunha E R,Blume G R,et al.Cryopreservation of boar sperm comparing different cryoprotectants associated in media based on powdered coconut water,lactose and trehalose[J].Cryobiology,2015,70(2):90-94.
[13]Giaretta E,Estrada E,Bucci D,et al.Combining reduced glutathione and ascorbic acid has supplementary beneficial effects on boar sperm cryotolerance[J].Theriogenology,2015,83(3):399-407.
[14]Arraztoa C C,Miragaya M H,Chaves M G,et al.Porcine sperm vitrification I:cryoloops method[J].Andrologia,2017,49(7):e12706.
[15]Arraztoa C C,Miragaya M H,Chaves M G,et al.Porcine sperm vitrification II:Spheres method[J].Andrologia,2017,49(8):e12738.
[16]吴石坚,许典新.猪冷冻精液人工授精的推广与应用(1981-1988)[J].中国畜牧杂志,1990,26(4):22-24.
[17]殷方芝,戴建军,吴华莉,等.猪精液5 mL大管冷冻方法的初步研究[J].中国兽医学报,2009,29(4):519-522.
[18]郭堂玉,凌泽继,韦明宇,等.不同冷冻保护液和解冻温度对猪精液冷冻效果的影响[J].中国兽医学报,2010,30(7):1000-1004.
[19]张渭斌,孙世铎,刘丑生,等.猪0.5 mL细管冷冻精液的人工授精试验[J].西北农林科技大学学报:自然科学版,2011,39(10):47-52.
[20]陈明明,王开林,杨林昆,等.-80℃超低温冰箱冷冻保存猪精液的初步研究[J].黑龙江动物繁殖,2016,24(5):5-8.
[21]高秀丽,党燕娜,翟晓红,等.猪精液冷冻保护剂的研究进展[J].畜牧兽医杂志,2016,35(2):62-63.
[22]刘博洋.右旋糖酐、卡拉胶和羧甲基壳聚糖对猪精液冷冻保存效果的研究[D].杨凌:西北农林科技大学,2017.
[23]王捷,耿果霞,杨丽,等.芝麻酚对猪精液冷冻保存效果的影响[J].家畜生态学报,2018,39(6):57-61.
[24]任子利,宋天增,张健,等.红景天多糖对藏猪精液冻后品质及精子基因组DNA甲基化的影响[J].中国兽医学报,2017,37(7):1385-1388,1393.
[25]孙艳青,李青旺,耿果霞,等.葡萄籽原花青素对猪精液冷冻保存效果的研究[J].家畜生态学报,2016,37(5):46-51.
[26]Gadani B,Bucci D,Spinaci M,et al.Resveratrol and Epigallocatechin-3-gallate addition to thawed boar sperm improves in vitro fertilization[J].Theriogenology,2017,90:88.
[27]Lee E,Kim D.Effects of Astaxanthin on miniature pig sperm cryopreservation[J].Biomed Res Int,2018,4:6784591.
[28]Li J W,Parrilla I,Ortega M D,et al.Post-thaw boar sperm motility is affected by prolonged storage of sperm in liquid nitrogen.Aretrospective study[J].Cryobiology,2017,80:119-125.
[29]Byrne G P,Lonergan P,Wade M,et al.Effect of freezing rate of ram spermatozoa on subsequent fertility in vivo and in vitro[J].Anim Reprod Sci,2000,62(4):265-275.
[30]Johnson L A,Weitze K F,Fiser P,et al.Storage of boar semen[J].Anim Reprod Sci,2000,62(1-3):143-172.
[31]Yeste M.State-of-the-art of boar sperm preservation in liquid and frozen state[J].Anim Reprod,2017,14(1):69-81.
[32]Pinho R O,Lima D M A,Shiomi H H,et al.Effect of different cryoprotectants on the viability of frozen/thawed semen from boars of the Piau breed[J].Anim Reprod Sci,2014,146(3-4):187-192.
[33]张利坤,井辛宇,白腾,等.抗氧化剂对家畜精液冷冻保存效果的影响研究进展[J].畜牧兽医杂志,2018(5):37-41.
[34]Chatterjee S,Lamirande E D,Gagnon C.Cryopreservation alters membrane sulfhydryl status of bull spermatozoa:Protection by oxidized glutathione[J].Mol Reprod Dev,2001,60(4):498-506.
[35]Trzcin′Ska M,Bry?a M.Apoptotic-like changes of boar spermatozoa in freezing media supplemented with different antioxidants[J].Pol J Vet Sci,2015,18(3):473-480.
[36]Allai L,Benmoula A,Maia M D S,et al.Supplementation of ram semen extender to improve seminal quality and fertility rate[J].Anim Reprod Sci,2018:192.
[37]Iida I,Ikeda K.Studies on deep-freezing of boar semen.II.Effect of various rapid freezing rates on survival of boar spermatozoa[J].Jap J Zootech Sci,1966,37:417.
[38]Roca J,Rodríguezmartínez H,Vázquez J M,et al.Strategies to improve the fertility of frozen-thawed boar semen for artificial insemination[J].Soc Reprod Fertil Suppl,2006,62:261-275.
[39]Pursel V G.Proceddure for the preservation of boar spermatozoa by freezing[J].USDA ARS Bull,1971,44(227):1-5.
[40]Graham E F.Preliminary report procedure and rationale for freezing boar semen[J].AI Digest,1971,19:12-14.
[41]Park H K,Kim S H,Kim K J,et al.Studies on the frozen boar semen.I.Studies on the development of diluents for freezing of boar semen[J].Korean J Anim Sci,1977,19:260-266.
[42]Milovanov V K,Baranov F A,Qhil'tsova L S,et al.Developing methods for freezing boar semen[J].Zhivotnovodstvo,1974,3:66-71.
[43]Yeste M.State-of-the-art of boar sperm preservation in liquid and frozen state[J].Anim Reprod,2017,14(1):69-81.
[44]Zeng C,Tang K,He L,et al.Effects of glycerol on apoptotic signaling pathways during boar spermatozoa cryopreservation[J].Cryobiology,2014,68(3):395-404.
[45]李大吉.不同甘油浓度对冻融猪精液质量参数的影响[J].科技视界,2012(30):58.
[46]潘红梅,张亮,蓝静,等.不同双糖组合对猪精子冷冻保存效果的影响[J].中国农学通报,2015,31(2):35-39.
[47]Gómez-Fernández J,Gómez-Izquierdo E,Tomás C,et al.Effect of different monosaccharides and disaccharides on boar sperm quality after cryopreservation[J].Anim Reprod Sci,2012,133(1-2):109-116.
[48]Zech N H,Lejeune B,Zech H,et al.Vitrification of hatching and hatched human blastocysts:effect of an opening in the zona pellucida before vitrification[J].Reprod Biomed Online,2005,11(3):355-361.
[49]Checura C M,Seidel G E.Effect of macromolecules in solutions for vitrification of mature bovine oocytes[J].Theriogenology,2007,67(5):919-930.
[50]Clarke R N,Johnson L A.Effect of liquid storage and cryopreservation of boar spermatozoa on acrosomal integrity and the penetration of zona-free hamster ova in vitro[J].Mol Reprod Dev,1987,16(3):193-204.
[51]李林林.猪精液5 mL细管冷冻保存技术研究[D].雅安:四川农业大学,2006.
[52]Eriksson B M,Petersson H,Rodriguez-Martinez H.Field fertility with exported boar semen frozen in the new flatpack container[J].Theriogenology,2002,58(6):1065-1079.
[53]Waide Y.Fertility and survival of frozen boar spermatozoa stored in alminum packaging containers[J].Jarq Jap Agr Res Q,1975,9:115-119.
[54]Barbas J P,Mascarenhas R D.Cryopreservation of domestic animal sperm cells[J].Cell Tissue Bank,2009,10(1):49-62.
[55]殷方芝.猪精液5 mL大管冷冻保存方法的建立及优化[D].南京:南京农业大学,2008.
[56]Bwanga C O,Braganca M M,Einarsson S,et al.Cryopreservation of boar semen in mini-and maxi-straws[J].Transbound Emerg Dis,1990,37(1-10):651-658.
[57]刘德玉,杨尚雪,黄明光,等.猪精液冷冻保存的剂型及品种效应[J].湖北农业科学,2014,53(20):4917-4919.
[58]薛琦.细管类型和距液氮面高度对猪精液冷冻保存效果的影响[J].国外畜牧学(猪与禽),2018,38(4):57-60.
[59]周鑫,张曼玲,赵丽华,等.不同冷冻方法和解冻液对猪精液冷冻效率的影响[J].内蒙古大学学报:自然科学版,2013,44(1):80-86.
[60]梁鸿斌,姚学军,李维华,等.冷冻速率和解冻温度对猪精液冷冻效果的影响[J].中国畜牧兽医,2012,39(11):157-160.
[61]查星琴,肖晶,成文敏,等.版纳微型猪近交系精子冷冻保存研究[J].中国畜牧兽医,2017,44(1):167-172.
[2]Polge C,Salamon S,Wilmut I.Fertilizing capacity of frozen boar semen following surgical insemination[J].Vet Rec,1970,87:424-429.
[3]Pursel V G,Johnson L A.Freezing of boar spermatozoa:fertilizing capacity with concentrated semen and a new thawing procedure[J].J Anim Sci,1975,40(1):99-102.
[4]Westendorf P,Richter L,Treu H.Deep freezing of boar sperma.Laboratory and insemination results using the Hülsenberger paillete method[J].Deut Tierarztl Woch,1975,82(7):261.
[5]Almlid T,Johnson L A.Effects of glycerol concentration,equilibration time and temperature of glycerol addition on postthaw viability of boar spermatozoa frozen in straws 1[J].JAnim Sci,1988,66(11):2899-2905.
[6]Fiser P S,Fairfull R W.Combined effect of glycerol concentration and cooling velocity on motility and acrosomal integrity of boar spermatozoa frozen in 0.5 ml straws[J].Mol Reprod Dev,1990,25(2):123-129.
[7]Córdova A,Ducolomb Y,Jiménez I,et al.In vitro fertilizing capacity of frozen-thawed boar semen[J].Theriogenology,1997,47(7):1309-1317.
[8]Petrounkina A M,Harrison R A,Petzoldt R,et al.Cyclical changes in sperm volume during in vitro incubation under capacitating conditions:a novel boar semen characteristic[J].J Reprod Fert,2000,118(2):283.
[9]Chanapiwat P,Kaeoket K,Tummaruk P.Cryopreservation of boar semen by egg yolk-based extenders containing lactose or fructose is better than sorbitol[J].J Vet Med Sci,2012,74(3):351-354.
[10]Okazaki T,Shimada M.New strategies of boar sperm cryopreservation:development of novel freezing and thawing methods with a focus on the roles of seminal plasma[J].Anim Sci J,2012,83(9):623-629.
[11]Tomás C,Gómez‐Fernández J,Gómez‐Izquierdo E,et al.Addition of cholesterol‐loaded cyclodextrins to the thawing extender:effects on boar sperm quality[J].Reprod Domest Anim,2014,49(3):427-432.
[12]Silva C G,Cunha E R,Blume G R,et al.Cryopreservation of boar sperm comparing different cryoprotectants associated in media based on powdered coconut water,lactose and trehalose[J].Cryobiology,2015,70(2):90-94.
[13]Giaretta E,Estrada E,Bucci D,et al.Combining reduced glutathione and ascorbic acid has supplementary beneficial effects on boar sperm cryotolerance[J].Theriogenology,2015,83(3):399-407.
[14]Arraztoa C C,Miragaya M H,Chaves M G,et al.Porcine sperm vitrification I:cryoloops method[J].Andrologia,2017,49(7):e12706.
[15]Arraztoa C C,Miragaya M H,Chaves M G,et al.Porcine sperm vitrification II:Spheres method[J].Andrologia,2017,49(8):e12738.
[16]吴石坚,许典新.猪冷冻精液人工授精的推广与应用(1981-1988)[J].中国畜牧杂志,1990,26(4):22-24.
[17]殷方芝,戴建军,吴华莉,等.猪精液5 mL大管冷冻方法的初步研究[J].中国兽医学报,2009,29(4):519-522.
[18]郭堂玉,凌泽继,韦明宇,等.不同冷冻保护液和解冻温度对猪精液冷冻效果的影响[J].中国兽医学报,2010,30(7):1000-1004.
[19]张渭斌,孙世铎,刘丑生,等.猪0.5 mL细管冷冻精液的人工授精试验[J].西北农林科技大学学报:自然科学版,2011,39(10):47-52.
[20]陈明明,王开林,杨林昆,等.-80℃超低温冰箱冷冻保存猪精液的初步研究[J].黑龙江动物繁殖,2016,24(5):5-8.
[21]高秀丽,党燕娜,翟晓红,等.猪精液冷冻保护剂的研究进展[J].畜牧兽医杂志,2016,35(2):62-63.
[22]刘博洋.右旋糖酐、卡拉胶和羧甲基壳聚糖对猪精液冷冻保存效果的研究[D].杨凌:西北农林科技大学,2017.
[23]王捷,耿果霞,杨丽,等.芝麻酚对猪精液冷冻保存效果的影响[J].家畜生态学报,2018,39(6):57-61.
[24]任子利,宋天增,张健,等.红景天多糖对藏猪精液冻后品质及精子基因组DNA甲基化的影响[J].中国兽医学报,2017,37(7):1385-1388,1393.
[25]孙艳青,李青旺,耿果霞,等.葡萄籽原花青素对猪精液冷冻保存效果的研究[J].家畜生态学报,2016,37(5):46-51.
[26]Gadani B,Bucci D,Spinaci M,et al.Resveratrol and Epigallocatechin-3-gallate addition to thawed boar sperm improves in vitro fertilization[J].Theriogenology,2017,90:88.
[27]Lee E,Kim D.Effects of Astaxanthin on miniature pig sperm cryopreservation[J].Biomed Res Int,2018,4:6784591.
[28]Li J W,Parrilla I,Ortega M D,et al.Post-thaw boar sperm motility is affected by prolonged storage of sperm in liquid nitrogen.Aretrospective study[J].Cryobiology,2017,80:119-125.
[29]Byrne G P,Lonergan P,Wade M,et al.Effect of freezing rate of ram spermatozoa on subsequent fertility in vivo and in vitro[J].Anim Reprod Sci,2000,62(4):265-275.
[30]Johnson L A,Weitze K F,Fiser P,et al.Storage of boar semen[J].Anim Reprod Sci,2000,62(1-3):143-172.
[31]Yeste M.State-of-the-art of boar sperm preservation in liquid and frozen state[J].Anim Reprod,2017,14(1):69-81.
[32]Pinho R O,Lima D M A,Shiomi H H,et al.Effect of different cryoprotectants on the viability of frozen/thawed semen from boars of the Piau breed[J].Anim Reprod Sci,2014,146(3-4):187-192.
[33]张利坤,井辛宇,白腾,等.抗氧化剂对家畜精液冷冻保存效果的影响研究进展[J].畜牧兽医杂志,2018(5):37-41.
[34]Chatterjee S,Lamirande E D,Gagnon C.Cryopreservation alters membrane sulfhydryl status of bull spermatozoa:Protection by oxidized glutathione[J].Mol Reprod Dev,2001,60(4):498-506.
[35]Trzcin′Ska M,Bry?a M.Apoptotic-like changes of boar spermatozoa in freezing media supplemented with different antioxidants[J].Pol J Vet Sci,2015,18(3):473-480.
[36]Allai L,Benmoula A,Maia M D S,et al.Supplementation of ram semen extender to improve seminal quality and fertility rate[J].Anim Reprod Sci,2018:192.
[37]Iida I,Ikeda K.Studies on deep-freezing of boar semen.II.Effect of various rapid freezing rates on survival of boar spermatozoa[J].Jap J Zootech Sci,1966,37:417.
[38]Roca J,Rodríguezmartínez H,Vázquez J M,et al.Strategies to improve the fertility of frozen-thawed boar semen for artificial insemination[J].Soc Reprod Fertil Suppl,2006,62:261-275.
[39]Pursel V G.Proceddure for the preservation of boar spermatozoa by freezing[J].USDA ARS Bull,1971,44(227):1-5.
[40]Graham E F.Preliminary report procedure and rationale for freezing boar semen[J].AI Digest,1971,19:12-14.
[41]Park H K,Kim S H,Kim K J,et al.Studies on the frozen boar semen.I.Studies on the development of diluents for freezing of boar semen[J].Korean J Anim Sci,1977,19:260-266.
[42]Milovanov V K,Baranov F A,Qhil'tsova L S,et al.Developing methods for freezing boar semen[J].Zhivotnovodstvo,1974,3:66-71.
[43]Yeste M.State-of-the-art of boar sperm preservation in liquid and frozen state[J].Anim Reprod,2017,14(1):69-81.
[44]Zeng C,Tang K,He L,et al.Effects of glycerol on apoptotic signaling pathways during boar spermatozoa cryopreservation[J].Cryobiology,2014,68(3):395-404.
[45]李大吉.不同甘油浓度对冻融猪精液质量参数的影响[J].科技视界,2012(30):58.
[46]潘红梅,张亮,蓝静,等.不同双糖组合对猪精子冷冻保存效果的影响[J].中国农学通报,2015,31(2):35-39.
[47]Gómez-Fernández J,Gómez-Izquierdo E,Tomás C,et al.Effect of different monosaccharides and disaccharides on boar sperm quality after cryopreservation[J].Anim Reprod Sci,2012,133(1-2):109-116.
[48]Zech N H,Lejeune B,Zech H,et al.Vitrification of hatching and hatched human blastocysts:effect of an opening in the zona pellucida before vitrification[J].Reprod Biomed Online,2005,11(3):355-361.
[49]Checura C M,Seidel G E.Effect of macromolecules in solutions for vitrification of mature bovine oocytes[J].Theriogenology,2007,67(5):919-930.
[50]Clarke R N,Johnson L A.Effect of liquid storage and cryopreservation of boar spermatozoa on acrosomal integrity and the penetration of zona-free hamster ova in vitro[J].Mol Reprod Dev,1987,16(3):193-204.
[51]李林林.猪精液5 mL细管冷冻保存技术研究[D].雅安:四川农业大学,2006.
[52]Eriksson B M,Petersson H,Rodriguez-Martinez H.Field fertility with exported boar semen frozen in the new flatpack container[J].Theriogenology,2002,58(6):1065-1079.
[53]Waide Y.Fertility and survival of frozen boar spermatozoa stored in alminum packaging containers[J].Jarq Jap Agr Res Q,1975,9:115-119.
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