摘要
目的建立分子印迹固相萃取—高效液相色谱法同时测定辣椒制品中4种苏丹红(苏丹红Ⅰ、Ⅱ、Ⅲ和Ⅳ)的含量。方法改进GB/T19681-2005食品中苏丹红染料的检测方法高效液相色谱法中的试样处理方法,使用Welchrom~?SDH分子印迹固相萃取小柱对多种辣椒制品基质进行净化,并采用Ultimate~?XB-C18(250 mm×4.6 mm, 5μm)色谱柱,以乙腈和水为流动相进行等度洗脱,于500 nm波长下测定。结果苏丹红Ⅰ、Ⅱ、Ⅲ和Ⅳ在0.1~10.0μg/mL范围内线性关系良好,相关系数r~2为0.9995~0.9999,回收率为88.9%~106.6%,检出限为0.01 mg/kg。结论该方法简便、灵敏度高、准确性好,适用于食品中4种苏丹红的定量分析。
Objective To establish a method for simultaneous determination of 4 kinds of Sudan red in chili products by molecular imprinted solid phase extraction-high performance liquid chromatography. Methods GB/T 19681-2005 Method for the detection of Sudan red dyes in food-High performance liquid chromatography was improved, and various chili product substrates were purified using Welchrom~? SDH molecularly imprinted solid phase extraction column. Samples were separated with a Ultimate~? XB-C18(250 mm×4.6 mm, 5 μm) column by isocratic elution using acetonitrile and water as the mobile phase and detected at 500 nm. Results Sudan red I, II, III and IV had a good linear relationship in the range of 0.1-10.0 μg/mL, the correlation coefficients r~2 were 0.9995-0.9999, the recovery rates were 88.9%-106.6%, and the limit of detection was 0.01 mg/kg. Conclusion This method is simple, sensitive, accurate, and it is suitable for the quantitative analysis of 4 kinds of Sudan red in chili products sample at the same time.
引文
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