油用牡丹籽粕低聚茋类化合物大孔树脂富集与抑菌活性
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摘要
以牡丹籽粕粗提物为原料,用大孔吸附树脂富集低聚茋类化合物,并借助抑菌圈试验研究纯化后低聚茋类化合物的抑菌活性。结果表明,HPD-100型大孔吸附树脂比AB-8更适合牡丹籽粕中低聚茋类化合物的纯化。最佳吸附工艺为:上样液质量浓度为1.15 mg/m L,上样流速为2 BV/h,上样量为100 m L;最佳洗脱工艺为:乙醇体积分数为60%,洗脱体积为8 BV,流速为3 BV/h。在最优试验条件下,牡丹籽粕中低聚茋类化合物的质量分数可达39.52%。抑菌活性试验表明,经树脂富集后的低聚茋类化合物抑菌活性良好。该研究结果可为高效利用油用牡丹资源提供理论基础。
In this paper, the crude extracts of peony seed were used as raw materials, and macroporous adsorption resin was used to enrich and purify the oligostilbenes in the peony seed cake. The inhibitory effect of oligostilbene extracts before and after enrichment on bacteria was studied. The results showed that compared with AB-8 macroporous adsorption resin, HPD-100 was more suitable for the purification of oligostilbenes from peony seed cake. The best adsorption process was: liquid mass concentration was 1.15 mg/m L, the sample flow rate was 2 BV/h, and the sample volume was 100 m L. The best elution procedure was: ethanol volume fraction 60%, elution volume 8 BV, and flow rate 3 BV/h. Under the optimal conditions, the peony seed cake in the mass fraction of low poly stilbene compouds could reach 39.52%. The antibacterial experiment results showed that the oligostilbenes compounds after the resin enrichment had good antibacterial activity. The research results lay a theoretical foundation for the effective utilization of oil peony resources.
In this paper, the crude extracts of peony seed were used as raw materials, and macroporous adsorption resin was used to enrich and purify the oligostilbenes in the peony seed cake. The inhibitory effect of oligostilbene extracts before and after enrichment on bacteria was studied. The results showed that compared with AB-8 macroporous adsorption resin, HPD-100 was more suitable for the purification of oligostilbenes from peony seed cake. The best adsorption process was: liquid mass concentration was 1.15 mg/m L, the sample flow rate was 2 BV/h, and the sample volume was 100 m L. The best elution procedure was: ethanol volume fraction 60%, elution volume 8 BV, and flow rate 3 BV/h. Under the optimal conditions, the peony seed cake in the mass fraction of low poly stilbene compouds could reach 39.52%. The antibacterial experiment results showed that the oligostilbenes compounds after the resin enrichment had good antibacterial activity. The research results lay a theoretical foundation for the effective utilization of oil peony resources.
引文
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[14]伍好.肉鸡源性肠杆菌科细菌中耐药基因、整合子及ISCR1元件的研究[D].济南:山东大学,2016.
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[16]申少斐,裴燕妮,张丽,等.油用牡丹籽仁中茋类化合物超声波提取及其抑菌活性研究[J].山西农业科学,2018,46(5):742-747.
[17]黄燕秋,谢志茹,朴胜华,等.大孔吸附树脂在中药纯化中的应用进展[J].广东化工,2018,45(8):130-132.
[2]韩长志,左安建,刘云霞.油用牡丹研究进展[J].北方园艺,2016(20):194-197.
[3]陈景震,李培旺,张良波.湖南油用牡丹籽油的理化性质及脂肪酸组分分析[J].经济林研究,2015,33(4):119-121.
[4]张喜雨,周军,晁燕,等.四种天然食用植物油脂的脂肪酸成分分析[J].湖南林业科技,2015(3):69-71.
[5]王顺利,任秀霞,薛璟祺,等.牡丹籽油成分、功效及加工工艺的研究进展[J].中国粮油学报,2016,31(3):139-146.
[6]牛亚琪.油用牡丹籽榨油副产物低聚茋类和单萜苷类化合物提取与纯化研究[D].洛阳:河南科技大学,2015.
[7]田潇.凤丹籽化学成分和抗肿瘤活性研究[D].西安:西北大学,2017.
[8]刘普,牛亚琪,邓瑞雪,等.紫斑牡丹籽粕低聚芪类成分研究[J].中国药学杂志,2014,49(12):1018-1021.
[9]沈瑞.闪式提取在中药中的应用进展[J].中药材,2015,38(7):1540-1542.
[10]刘普,李小方,王永威,等.油用牡丹籽粕中低聚茋类含量测定方法研究[J].中国粮油学报,2016,31(4):138-141,146.
[11]刘普,许艺凡,李小方,等.油用牡丹籽壳低聚茋类化合物富集及对牡丹籽油抗氧化作用研究[J].中国粮油学报,2017,32(6):84-90.
[12] LIU P,LI X F,GAO J Y,et al. Two new resveratrol trimers with antibacterial activities from seed cake of Paeonia rockii[J]. Chemistry of Natural Compounds,2017,53(1):51-55.
[13]袁红宇,欧宁,朱璇,等.大孔树脂纯化买麻藤总茋化合物的工艺研究[J].中成药,2010,32(11):1992-1995.
[14]伍好.肉鸡源性肠杆菌科细菌中耐药基因、整合子及ISCR1元件的研究[D].济南:山东大学,2016.
[15]翟梅枝,问小强,刘枫,等.核桃属植物内生真菌的分离及其抑菌活性研究[J].西北林学院学报,2009(3):144-147.
[16]申少斐,裴燕妮,张丽,等.油用牡丹籽仁中茋类化合物超声波提取及其抑菌活性研究[J].山西农业科学,2018,46(5):742-747.
[17]黄燕秋,谢志茹,朴胜华,等.大孔吸附树脂在中药纯化中的应用进展[J].广东化工,2018,45(8):130-132.