还原型谷胱甘肽调控shewanella oneidensis MR-1降解亚碲酸盐
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摘要
探讨了生物活性小分子还原型谷胱甘肽(GSH)调控奥奈达湖希瓦氏菌(shewanella oneidensis)MR-1还原亚碲酸盐的特性及机理.结果表明,生物体系中加入0.1,0.4, 1.0mmol/L GSH,与空白对照相比,亚碲酸盐的生物还原效率可分别提高55%、71%和78%,且GSH浓度在0.1～1.0mmol/L范围内与亚碲酸盐的生物还原效率呈正相关.采用单因素实验优化了培养条件,在温度35℃,pH 8.0,GSH浓度为0.4mmol/L的条件下,亚碲酸盐生物还原效率在24h内即可达到97%.采用6种不同呼吸抑制剂实验探讨亚碲酸盐生物还原的电子传递路径,确定GSH在亚碲酸盐生物还原电子传递链上的加速位点为NADH还原酶、甲萘醌和FAD脱氢酶.
The regulated characteristics and mechanism of tellurite bioreduction by bioactive small molecule glutathione(GSH) withShewanella oneidensis MR-1 were investigated in this study. When 0.1, 0.4 and 1.0 mmol/L GSH were added to the bioreduction system, the bioreduction efficiency of the tellurite was increased by 55%, 71% and 78%, respectively, compared with the control. Moreover, the concentration of GSH(0.1～1.0 mmol/L) was positively correlated with the bioreduction efficiency of tellurite. The culture conditions were optimized by single factor experiment. Under the conditions of 35°C, pH 8.0, 0.4 mmol/L GSH, the bioreduction efficiency of tellurite reached 97% within 24 h. In addition, six different respiratory inhibitors were used to investigate the regulated electron transfer pathway of tellurite bioreduction by GSH. It was preliminarily determined that the accelerating sites of GSH in the electron transfer chain were NADH reductase, methylnaphthoquinone and FAD dehydrogenase.
The regulated characteristics and mechanism of tellurite bioreduction by bioactive small molecule glutathione(GSH) withShewanella oneidensis MR-1 were investigated in this study. When 0.1, 0.4 and 1.0 mmol/L GSH were added to the bioreduction system, the bioreduction efficiency of the tellurite was increased by 55%, 71% and 78%, respectively, compared with the control. Moreover, the concentration of GSH(0.1～1.0 mmol/L) was positively correlated with the bioreduction efficiency of tellurite. The culture conditions were optimized by single factor experiment. Under the conditions of 35°C, pH 8.0, 0.4 mmol/L GSH, the bioreduction efficiency of tellurite reached 97% within 24 h. In addition, six different respiratory inhibitors were used to investigate the regulated electron transfer pathway of tellurite bioreduction by GSH. It was preliminarily determined that the accelerating sites of GSH in the electron transfer chain were NADH reductase, methylnaphthoquinone and FAD dehydrogenase.
引文
[1]Ramosruiz A,Sesmamartin J,Sierraalvarez R,et al.Continuous reduction of tellurite to recoverable tellurium nanoparticles using an upflow anaerobic sludge bed(UASB)reactor[J].Water Research,2016,108(1):189-196.
[2]Turner R J,Borghese R,Zannoni D.Microbial processing of tellurium as a tool in biotechnology[J].Biotechnology Advances,2012,30(5):954-963.
[3]Ramosruiz A,Field J A,Wilkening J V,et al.Recovery of Elemental Tellurium Nanoparticles by the Reduction of Tellurium Oxyanions in a Methanogenic Microbial Consortium[J].Environmental Science&Technology,2016,50(3):1492-1500.
[4]胡莉萍,吕京,彭开良,等.碲及其化合物的毒性研究进展[J].毒理学杂志,2002,16(2):120-123.Liping Hu,Jing Lu,Kailiang Peng,et al.Progress in toxicity of ruthenium and its compounds[J].Journal of Toxicology,2002,16(2):120-123.
[5]喻涌泉,黄魏魏,董建江,等.硝基还原假单胞菌吸附重金属镉的机理研究[J].中国环境科学,2017,37(6):2232-2238.Yongquan Yu,Weiwei Huang,Jianjiang Dong,et al.Study on the mechanism of adsorption of heavy metal cadmium by nitro-reducing Pseudomonas[J].China Environmental Science,2017,37(6):2232-2238.
[6]张建梅.重金属废水处理技术研究进展(综述)[J].西安文理学院学报(社会科学版),2003,6(2):55-59.Jianmei Zhang.Research Progress in Treatment Technology of Heavy Metal Wastewater(Review)[J].Journal of Xi\'an University of Arts and Sciences(Social Science Edition),2003,6(2):55-59.
[7]Kim D H,Kanaly R A,Hur H G.Biological accumulation of tellurium nanorod structures via reduction of tellurite by Shewanella oneidensis MR-1[J].Bioresource Technology,2012,125(338):127-131.
[8]Borsetti F,Borghese R,Cappelletti M,et al.Tellurite processing by cells of Rhodobacter capsulatus,involves a periplasmic step where the oxyanion causes a malfunction of the cytochrome c maturation system[J].International Biodeterioration&Biodegradation,2018,130(5):84-90.
[9]Wang X,Liu G,Zhou J,et al.Quinone-mediated reduction of selenite and tellurite by Escherichia coli[J].Bioresource Technology,2011,102(3):3268-3271.
[10]Espinosa-Ortiz E J,Rene E R,Guyot F,et al.Biomineralization of tellurium and selenium-tellurium nanoparticles by the white-rot fungus Phanerochaete chrysosporium[J].International Biodeterioration&Biodegradation,2017,124(2):258-266.
[11]Zee F P V D,Cervantes F J.Impact and application of electron shuttles on the redox(bio)transformation of contaminants:A review[J].Biotechnology Advances,2009,27(3):256-277.
[12]张隆,王竞,周集体,等.酸性偶氮染料还原产物强化偶氮染料生物脱色[J].中国环境科学,2008,28(3):237-241.Long Zhang,Jing Wang,Jiti Zhou,et al.Biodecolorization of azo dyes by acid azo dye reduction products[J].China Environmental Science,2008,28(3):237-241.
[13]Chen M,Wen Q,Gu F,et al.Mussel Chemistry Assembly of a Novel Biosensing Nanoplatform Based on Polydopamine Fluorescent Dot and Its Photophysical Features[J].Chemical Engineering Journal,2018,342(6):331-338.
[14]刘慧,王晓蓉,王为木,等.不同形态锌离子对鲫鱼谷胱甘肽系统的影响[J].中国环境科学,2005,25(2):169-173.Hui Liu,Xiaorong Wang,Weimu Wang,et al.Effects of Different Forms of Zinc Ions on Glutathione System in Carp[J].China Environmental Science,2005,25(2):169-173.
[15]Jozefczak M,Remans T,Vangronsveld J,et al.Glutathione Is a Key Player in Metal-Induced Oxidative Stress Defenses[J].International Journal of Molecular Sciences,2012,13(3):3145-3175.
[16]杜艳影,刘小红,李劲,等.Shewanella oneidensis MR-1对Cr(Ⅵ)的还原及其影响因素[J].中国环境科学,2018,38(7):2740-2745.Yanying Du,Xiaohong Liu,Jin Li,et al.Reduction of Cr(VI)by Shewanella oneidensis MR-1 and its influencing factors[J].China Environmental Science,2018,38(7):2740-2745.
[17]Noctor G,Foyer C H.Ascorbate and Glutathione:Keeping Active Oxygen Under Control[J].Annual Reviews Plant Physiology Plant Molecue Biology,1998,49(49):249-279.
[18]Li D B,Cheng Y Y,Wu C,et al.Selenite reduction by Shewanella oneidensis MR-1is mediated by fumarate reductase in periplasm[J].Scientific Reports,2014,4(6168):3735.
[19]Yu Q,Xi H,Chen B,et al.In vitro,Study on Antagonism Mechanism of Glutathione,Sodium Selenite and Mercuric Chloride[J].Talanta,2017,171(8):262-269.
[20]Yoon J H,Yeo S H,Kim I G,et al.Shewanella marisflavi sp.nov.and Shewanella aquimarina sp.nov.,slightly halophilic organisms isolated from sea water of the yellow sea in Korea[J].International Journal of Systematic and Evolutionary microbiology,2004,54(6):2347-2352.
[21]李俊江,宋圆圆,刘雁滨,等.氧化还原介体催化强化Shewanella oneidensis MR-1还原亚硒酸盐的研究[J].河北科技大学学报,2018,39(1):73-83.Junjiang Li,Yuanyuan Song,Yanbin Liu,et al.Study on the reduction of selenite by Shewanella oneidensis MR-1catalyzed by redox mediator[J].Journal of Hebei University of Science and Technology,2018,39(1):73-83.
[22]贾丹,李卓然,钟志国.pH对新型后置反硝化系统生物脱氮除磷的影响[J].水处理技术,2018,44(5):84-88.Dan Jia,Zhuoran Li,Zhiguo Zhong.Effect of pH on Biological Nitrogen and Phosphorus Removal in a Novel Post-Denitrification System[J].Water Treatment Technology,2018,44(5):84-88.
[23]Leaphart A B,Thompson D K,Huang K,et al.Transcriptome Profiling of Shewanella oneidensis Gene Expression following Exposure to Acidic and Alkaline pH[J].Journal of Bacteriology,2006,188(4):1633-1642.
[24]Rigobello M P,Folda A,Citta A,et al.Interaction of selenite and tellurite with thiol-dependent redox enzymes:Kinetics and mitochondrial implications[J].Free Radical Biology Medicine,2011,50(11):1620-1629.
[25]刘金香.奥奈达希瓦氏菌铀(Ⅵ)还原能力的增强机制及应用基础研究[D].衡阳:南华大学,2015.Jinxiang Liu.Enhancement mechanism and application of uranium(VI)reducing ability of A.sinensis[D].Hengyang:Nanhua University,2015.
[26]Helbig K,Bleuel C,Krauss G J,et al.Glutathione and TransitionMetal Homeostasis in Escherichia coli[J].Journal of Bacteriology,2008,190(15):5431-5438.
[27]Wang X,Liu G,Zhou J,et al.Quinone-mediated reduction of selenite and tellurite by Escherichia coli[J].Bioresource Technology,2011,102(3):3268-3271.
[28]Yamada A,Miyashita M,Inoue K,et al.Extracellular reduction of selenite by a novel marine photosynthetic bacterium[J].Applied Microbiology&Biotechnology,1997,48(3):367-372.
[29]Wo?nica A,Dzirba J,Mańka D,et al.Effects of electron transport inhibitors on iron reduction in Aeromonas hydrophila strain KB1[J].Anaerobe,2003,9(3):125-130.
[30]Wang S,Guo J,Lian J,et al.Rapid start-up of the anammox process by denitrifying granular sludge and the mechanism of the anammox electron transport chain[J].Biochemical Engineering Journal,2016,115(11):101-107.
[31]Ma C,Yang G Q,Lu Q,et al.Anaerobic reduction of humus/Fe(III)and electron transport mechanism of Fontibacter sp.SgZ-2[J].Environmental.Science,2014,35(9):3522-3529.
[32]Lian J,Tian X,Guo J,et al.Effects of resazurin on perchlorate reduction and bioelectricity generation in microbial fuel cells and its catalysing mechanism[J].Biochemical Engineering Journal,2016,114(10):164-172.
[33]Fernandez,V M,Rua M L,Reyes P,Cammack R,Hatchikian E C(2010)Inhibition of Desulfovibrio gigas hydrogenase with copper salts and other metal ions.Eur J Biochem,185:449-454.
[34]Borghese R,Brucale M,Fortunato G,et al.Extracellular production of tellurium nanoparticles by the photosynthetic bacterium Rhodobacter capsulatus[J].Journal of Hazardous Materials,2016,309(Pt A):202-209.
[35]Lampis S,Zonaro E,Bertolini C,et al.Delayed formation of zero-valent selenium nanoparticles by Bacillus mycoides,SeITE01as a consequence of selenite reduction under aerobic conditions[J].Microbial Cell Factories,2014,13(1):35-35.
[2]Turner R J,Borghese R,Zannoni D.Microbial processing of tellurium as a tool in biotechnology[J].Biotechnology Advances,2012,30(5):954-963.
[3]Ramosruiz A,Field J A,Wilkening J V,et al.Recovery of Elemental Tellurium Nanoparticles by the Reduction of Tellurium Oxyanions in a Methanogenic Microbial Consortium[J].Environmental Science&Technology,2016,50(3):1492-1500.
[4]胡莉萍,吕京,彭开良,等.碲及其化合物的毒性研究进展[J].毒理学杂志,2002,16(2):120-123.Liping Hu,Jing Lu,Kailiang Peng,et al.Progress in toxicity of ruthenium and its compounds[J].Journal of Toxicology,2002,16(2):120-123.
[5]喻涌泉,黄魏魏,董建江,等.硝基还原假单胞菌吸附重金属镉的机理研究[J].中国环境科学,2017,37(6):2232-2238.Yongquan Yu,Weiwei Huang,Jianjiang Dong,et al.Study on the mechanism of adsorption of heavy metal cadmium by nitro-reducing Pseudomonas[J].China Environmental Science,2017,37(6):2232-2238.
[6]张建梅.重金属废水处理技术研究进展(综述)[J].西安文理学院学报(社会科学版),2003,6(2):55-59.Jianmei Zhang.Research Progress in Treatment Technology of Heavy Metal Wastewater(Review)[J].Journal of Xi\'an University of Arts and Sciences(Social Science Edition),2003,6(2):55-59.
[7]Kim D H,Kanaly R A,Hur H G.Biological accumulation of tellurium nanorod structures via reduction of tellurite by Shewanella oneidensis MR-1[J].Bioresource Technology,2012,125(338):127-131.
[8]Borsetti F,Borghese R,Cappelletti M,et al.Tellurite processing by cells of Rhodobacter capsulatus,involves a periplasmic step where the oxyanion causes a malfunction of the cytochrome c maturation system[J].International Biodeterioration&Biodegradation,2018,130(5):84-90.
[9]Wang X,Liu G,Zhou J,et al.Quinone-mediated reduction of selenite and tellurite by Escherichia coli[J].Bioresource Technology,2011,102(3):3268-3271.
[10]Espinosa-Ortiz E J,Rene E R,Guyot F,et al.Biomineralization of tellurium and selenium-tellurium nanoparticles by the white-rot fungus Phanerochaete chrysosporium[J].International Biodeterioration&Biodegradation,2017,124(2):258-266.
[11]Zee F P V D,Cervantes F J.Impact and application of electron shuttles on the redox(bio)transformation of contaminants:A review[J].Biotechnology Advances,2009,27(3):256-277.
[12]张隆,王竞,周集体,等.酸性偶氮染料还原产物强化偶氮染料生物脱色[J].中国环境科学,2008,28(3):237-241.Long Zhang,Jing Wang,Jiti Zhou,et al.Biodecolorization of azo dyes by acid azo dye reduction products[J].China Environmental Science,2008,28(3):237-241.
[13]Chen M,Wen Q,Gu F,et al.Mussel Chemistry Assembly of a Novel Biosensing Nanoplatform Based on Polydopamine Fluorescent Dot and Its Photophysical Features[J].Chemical Engineering Journal,2018,342(6):331-338.
[14]刘慧,王晓蓉,王为木,等.不同形态锌离子对鲫鱼谷胱甘肽系统的影响[J].中国环境科学,2005,25(2):169-173.Hui Liu,Xiaorong Wang,Weimu Wang,et al.Effects of Different Forms of Zinc Ions on Glutathione System in Carp[J].China Environmental Science,2005,25(2):169-173.
[15]Jozefczak M,Remans T,Vangronsveld J,et al.Glutathione Is a Key Player in Metal-Induced Oxidative Stress Defenses[J].International Journal of Molecular Sciences,2012,13(3):3145-3175.
[16]杜艳影,刘小红,李劲,等.Shewanella oneidensis MR-1对Cr(Ⅵ)的还原及其影响因素[J].中国环境科学,2018,38(7):2740-2745.Yanying Du,Xiaohong Liu,Jin Li,et al.Reduction of Cr(VI)by Shewanella oneidensis MR-1 and its influencing factors[J].China Environmental Science,2018,38(7):2740-2745.
[17]Noctor G,Foyer C H.Ascorbate and Glutathione:Keeping Active Oxygen Under Control[J].Annual Reviews Plant Physiology Plant Molecue Biology,1998,49(49):249-279.
[18]Li D B,Cheng Y Y,Wu C,et al.Selenite reduction by Shewanella oneidensis MR-1is mediated by fumarate reductase in periplasm[J].Scientific Reports,2014,4(6168):3735.
[19]Yu Q,Xi H,Chen B,et al.In vitro,Study on Antagonism Mechanism of Glutathione,Sodium Selenite and Mercuric Chloride[J].Talanta,2017,171(8):262-269.
[20]Yoon J H,Yeo S H,Kim I G,et al.Shewanella marisflavi sp.nov.and Shewanella aquimarina sp.nov.,slightly halophilic organisms isolated from sea water of the yellow sea in Korea[J].International Journal of Systematic and Evolutionary microbiology,2004,54(6):2347-2352.
[21]李俊江,宋圆圆,刘雁滨,等.氧化还原介体催化强化Shewanella oneidensis MR-1还原亚硒酸盐的研究[J].河北科技大学学报,2018,39(1):73-83.Junjiang Li,Yuanyuan Song,Yanbin Liu,et al.Study on the reduction of selenite by Shewanella oneidensis MR-1catalyzed by redox mediator[J].Journal of Hebei University of Science and Technology,2018,39(1):73-83.
[22]贾丹,李卓然,钟志国.pH对新型后置反硝化系统生物脱氮除磷的影响[J].水处理技术,2018,44(5):84-88.Dan Jia,Zhuoran Li,Zhiguo Zhong.Effect of pH on Biological Nitrogen and Phosphorus Removal in a Novel Post-Denitrification System[J].Water Treatment Technology,2018,44(5):84-88.
[23]Leaphart A B,Thompson D K,Huang K,et al.Transcriptome Profiling of Shewanella oneidensis Gene Expression following Exposure to Acidic and Alkaline pH[J].Journal of Bacteriology,2006,188(4):1633-1642.
[24]Rigobello M P,Folda A,Citta A,et al.Interaction of selenite and tellurite with thiol-dependent redox enzymes:Kinetics and mitochondrial implications[J].Free Radical Biology Medicine,2011,50(11):1620-1629.
[25]刘金香.奥奈达希瓦氏菌铀(Ⅵ)还原能力的增强机制及应用基础研究[D].衡阳:南华大学,2015.Jinxiang Liu.Enhancement mechanism and application of uranium(VI)reducing ability of A.sinensis[D].Hengyang:Nanhua University,2015.
[26]Helbig K,Bleuel C,Krauss G J,et al.Glutathione and TransitionMetal Homeostasis in Escherichia coli[J].Journal of Bacteriology,2008,190(15):5431-5438.
[27]Wang X,Liu G,Zhou J,et al.Quinone-mediated reduction of selenite and tellurite by Escherichia coli[J].Bioresource Technology,2011,102(3):3268-3271.
[28]Yamada A,Miyashita M,Inoue K,et al.Extracellular reduction of selenite by a novel marine photosynthetic bacterium[J].Applied Microbiology&Biotechnology,1997,48(3):367-372.
[29]Wo?nica A,Dzirba J,Mańka D,et al.Effects of electron transport inhibitors on iron reduction in Aeromonas hydrophila strain KB1[J].Anaerobe,2003,9(3):125-130.
[30]Wang S,Guo J,Lian J,et al.Rapid start-up of the anammox process by denitrifying granular sludge and the mechanism of the anammox electron transport chain[J].Biochemical Engineering Journal,2016,115(11):101-107.
[31]Ma C,Yang G Q,Lu Q,et al.Anaerobic reduction of humus/Fe(III)and electron transport mechanism of Fontibacter sp.SgZ-2[J].Environmental.Science,2014,35(9):3522-3529.
[32]Lian J,Tian X,Guo J,et al.Effects of resazurin on perchlorate reduction and bioelectricity generation in microbial fuel cells and its catalysing mechanism[J].Biochemical Engineering Journal,2016,114(10):164-172.
[33]Fernandez,V M,Rua M L,Reyes P,Cammack R,Hatchikian E C(2010)Inhibition of Desulfovibrio gigas hydrogenase with copper salts and other metal ions.Eur J Biochem,185:449-454.
[34]Borghese R,Brucale M,Fortunato G,et al.Extracellular production of tellurium nanoparticles by the photosynthetic bacterium Rhodobacter capsulatus[J].Journal of Hazardous Materials,2016,309(Pt A):202-209.
[35]Lampis S,Zonaro E,Bertolini C,et al.Delayed formation of zero-valent selenium nanoparticles by Bacillus mycoides,SeITE01as a consequence of selenite reduction under aerobic conditions[J].Microbial Cell Factories,2014,13(1):35-35.