低蛋白质饲粮补充缬氨酸对肉鸡生长性能、屠宰性能和血清指标的影响

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英文篇名：Effects of Valine Supplementation in Low-Protein Diets on Growth Performance,Slaughter Performance and Serum Indices of Broilers 作者：陈将 ; 刘国华 ; PIRZADO ; Shoaib ; Ahmed ; 陈志敏 ; 郑爱娟 ; 常文环 ; 蔡辉益 ; 董晓丽 英文作者：CHEN Jiang;LIU Guohua;PIRZADO Shoaib Ahmed;CHEN Zhimin;ZHENG Aijuan;CHANG Wenhuan;CAI Huiyi;DONG Xiaoli;Key Open Laboratory of Feed Biotechnology of Ministry of Agriculture,Feed Research Institute,Chinese Academy of Agricultural Sciences;National Engineering Research Center for Biological Feed Development;Xijie (Shanghai) Trading Co.,Ltd.; 关键词：肉鸡 ; 缬氨酸 ; 生长性能 ; 血清指标 ; 血清游离氨基酸 英文关键词：broilers;;valine;;growth performance;;serum biochemical indices;;serum free amino acids 中文刊名：DWYX 英文刊名：Chinese Journal of Animal Nutrition 机构：中国农业科学院饲料研究所农业部饲料生物技术重点开放实验室;生物饲料开发国家工程研究中心;希杰(上海)商贸有限公司; 出版日期：2019-01-29 14:41 出版单位：动物营养学报 年：2019 期：v.31 基金：现代农业产业技术体系专项资金(CARS-41) 语种：中文; 页：DWYX201904017 页数：9 CN：04 ISSN：11-5461/S 分类号：141-149

摘要

本试验旨在研究低蛋白质饲粮补充缬氨酸(Val)对肉鸡生长性能、屠宰性能和血清指标的影响。选取300只1日龄爱拔益加肉鸡,随机分成5个组(Ⅰ～Ⅴ组),每组6个重复,每个重复10只鸡。试验各组饲粮赖氨酸(Lys)和粗蛋白质水平前期(1～21日龄)分别为1.17%和19.55%,后期(22～42日龄)分别为1.01%和17.67%;Ⅰ～Ⅴ组饲粮前期和后期Val/Lys值分别为68%、73%、78%、83%和88%。试验期42 d。结果表明:1)低蛋白质饲粮补充Val能显著提高1～42日龄肉鸡末体重和平均日增重(ADG)(P <0. 05),显著降低料重比(F/G)(P <0. 05),以ADG和F/G为效应指标进行二次曲线方程拟合表明,饲粮Val/Lys推荐值分别为80. 07%和81.03%。2)低蛋白质饲粮补充Val对肉鸡屠宰率、全净膛率、胸肌率、腿肌率和腹脂率均无显著影响(P>0.05)。3)与Ⅰ组相比,Ⅱ～Ⅴ组肉鸡血清中总蛋白、白蛋白、球蛋白含量以及总超氧化物歧化酶和过氧化氢酶活性均无显著差异(P> 0.05),但血清尿酸含量有所降低(0.05

0.05)。与Ⅰ组和Ⅱ组相比,Ⅲ组和Ⅳ组肉鸡血清中丙二醛含量显著降低(P<0.05)。低蛋白质饲粮补充Val对肉鸡血清游离氨基酸含量均无显著影响(P>0.05)。由此可见,低蛋白质饲粮补充Val能提高肉鸡生长性能和抗氧化能力。在本试验条件下,肉鸡获得最大ADG和最小F/G的饲粮Val/Lys推荐值分别为80.07%和81.03%。
        The objective of this study was to investigate the effects of valine( Val) supplementation in low-protein diets on growth performance,slaughter performance and serum indices of broilers. A total of 300 one-day-old Arbor Acres broilers were randomly divided into 5 groups( groups Ⅰ to Ⅴ) with 6 replicates per group and 10 chickens per replicate. The recommended levels of crude protein in diets for broilers in the starter( 1 to 21 days of age) and grower( 22 to 42 days of age) phases were 19. 55% and 17. 67%,respectively. The dietary lysine( Lys) levels were 1.17% and 1.01% for the starter and grower phases,respectively. Dietary Val/Lys ratios were supplied with 68%,73%,78%,83% and 88% for the starter and grower phases,respectively. The experiment lasted for 42 days. The results showed as follows: 1) the Val supplementation in low-protein diets significantly increased the final body weight and average daily gain( P < 0.05),but significantly decreased the feed/gain ratio( F/G)( P<0.05) of broilers from 1 to 42 days of age. When analyzed by quadratic regression model,the optimal Val/Lys ratios for ADG and F/G were 80. 07% and 81. 03%,respectively. 2) The Val supplementation in low-protein diets had no significantly different effects on slaughter rate,whole net carcass rate,breast muscle rate,leg muscle rate and abdominal fat rate of broilers( P>0.05). 3) Compared with group Ⅰ,the serum contents of total protein,albumin,globulin and the serum activates of total superoxide dismutase and catalase of broilers in groups Ⅱ to Ⅴ were not significantly different( P > 0. 05),but the serum uric acid content was decreased( 0.05 0. 05).The serum malondialdehyde content of broilers in groups Ⅲ and Ⅳ was significantly lower than that in groups Ⅰand Ⅱ( P> 0. 05). The Val supplementation in low-protein diets had no significantly different effects on serum free amino acid contents of broilers( P>0.05). These results indicate that the Val supplementation in low-protein diets can improve the growth performance and antioxidant capacity of broilers. The recommended Val/Lys ratios to achieve maximum ADG and minimum F/G are 80.07% and 81.03%,respectively.

引文

[1]MACK S,BERCOVICI D,DE GROOTE G,et al.Ideal amino acid profile and dietary lysine specification for broiler chickens of 20 to 40 days of age[J].British Poultry Science,1999,40(2):257-265.
    [2]BAKER D H,BATAL A B,PARR T M,et al.Ideal ratio(relative to lysine)of tryptophan,threonine,isoleucine,and valine for chicks during the second and third weeks posthatch[J].Poultry Science,2002,81(4):485-494.
    [3]徐琪寿.氨基酸药理学研究进展[J].氨基酸和生物资源,1996,18(1):30-32.
    [4]李根.饲粮中添加缬氨酸对泌乳母猪生产性能、血浆和乳汁生化指标的影响[D].硕士学位论文.广州:华南农业大学,2016:21.
    [5]杨小波.低蛋白日粮对肉仔鸡生长性能、血液生化指标和养分利用率的影响[D].硕士学位论文.沈阳:沈阳农业大学,2016:37-38.
    [6]PINCHASOV Y,MENDONCA C X,JENSEN L S.Broiler chick response to low protein diets supplemented with synthetic amino acids[J].Poultry Science,1990,69(11).1950-1955.
    [7]段晓翔.低蛋白日粮蛋氨酸水平对肉鸡氮代谢及肌肉生长相关基因表达的影响[D].硕士学位论文.长春:吉林大学,2015:12-13.
    [8]TAVERNARI F C,LELIS G R,VIEIRA R A,et al.Valine needs in starting and growing Cobb(500)broilers[J].Poultry Science,2013,92(1):151-157.
    [9]NASCIMENTO G R,MURAKAMI A E,OSPINA-RO-JAS I C,et al.Digestible valine requirements in lowprotein diets for broilers chicks[J].Brazilian Journal of Poultry Science,2016,18(3):381-386.
    [10]CORZO A,DOZIERⅢW A,KIDD M T.Valine nutrient recommendations for Ross×Ross 308 broilers[J].Poultry Science,2008,87(2):335-338.
    [11]BERRES J,VIEIRA S L,FAVERO A,et al.Digestible valine requirements in high protein diets for broilers from twenty-one to forty-two days of age[J].Animal Feed Science and Technology,2011,165(1/2):120-124.
    [12]刘绪同.生长肥育猪低氮排放日粮标准回肠可消化缬氨酸与赖氨酸适宜比例及对采食量调控的研究[D].博士学位论文.北京:中国农业大学,2016:18-19.
    [13]CORZO A,DOZIERⅢW A,LOARⅡR E,et al.Dietary limitation of isoleucine and valine in diets based on maize,soybean meal,and meat and bone meal for broiler chickens[J].British Poultry Science,2010,51(4):558-563.
    [14]DUARTE K F,JUNQUEIRA O M,DE FARIADOMINGUES C H,et al.Digestible valine requirements for broilers from 22 and 42 days old[J].Acta Scientiarum.Animal Sciences,2014,36(2):151-156.
    [15]陈熠.日粮中添加缬氨酸对哺乳母猪繁殖性能及血液和乳中相关指标的影响[D].硕士学位论文.长沙:湖南农业大学,2009:21.
    [16]AZZAM M M M,DONG X Y,XIE P,et al.The effect of supplemental L-threonine on laying performance,serum free amino acids,and immune function of laying hens under high-temperature and high-humidity environmental climates[J].The Journal of Applied Poultry Research,2011,20(3):361-370.
    [17]代腊,顾林英,朱巧明,等.饲粮缬氨酸水平对蛋鸡生产性能、蛋品质及血清生化指标的影响[J].动物营养学报,2012,24(4):654-660.
    [18]王勇.缬氨酸对高产哺乳母猪生产性能、免疫机能及氮利用率的影响研究[D].硕士学位论文.杭州:浙江大学,2010:53.
    [19]RICHERT B T,TOKACH M D,GOODBAND R D,et al.The effect of dietary lysine and valine fed during lactation on sow and litter performance[J].Journal of Animal Science,1997,75(7):1853-1860.
    [20]LENNARZ W J,LANE M D.Encyclopedia of biological chemistry[M].2nd ed.Amsterdam:Elsevier,2013:244-249.
    [21]ZHENG X C,WU Q J,SONG Z H,et al.Effects of Oridonin on growth performance and oxidative stress in broilers challenged with lipopolysaccharide[J].Poultry Science,2016,95(10):2281-2289.
    [22]GHISELLI A,SERAFINI M,NATELLA F,et al.Total antioxidant capacity as a tool to assess redox status:critical view and experimental data[J].Free Radical Biology and Medicine,2000,29(11):1106-1114.
    [23]ADIBI S A,MERCER D W.Protein digestion in human intestine as reflected in luminal,mucosal,and plasma amino acid concentrations after meals[J].Journal of Clinical Investigation,1973,52(7):1586-1594.
    [24]管武太.理想氨基酸模式提高猪生产性能的机理[D].博士学位论文.北京:中国农业大学,1997.
    [25]REN J B,ZHAO G Y,LI Y X,et al.Influence of dietary lysine level on whole-body protein turnover,plasma IGF-Ⅰ,GH and insulin concentration in growing pigs[J].Livestock Science,2007,110(1/2):126-132.
    [26]WILTAFSKY M K,PFAFFL M W,ROTH F X.The effects of branched-chain amino acid interactions on growth performance,blood metabolites,enzyme kinetics and transcriptomics in weaned pigs[J].British Journal of Nutrition,2010,103(7):964-976.