近红外光谱技术测定烤烟中NNK的含量
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摘要
选取国内5个省份462个烟叶样品,采用近红外光谱技术建立其4-(N-甲基-N-亚硝胺)-1-(3-吡啶基)-丁酮(NNK)的预测模型,并对模型进行外部检验。结果表明:(1)烟叶中总氮、烟碱、总糖和还原糖的含量与NNK含量呈现显著相关关系。(2)所建立的近红外方法测试烟叶NNK的相对平均误差分别为6. 88%; t配对检验结果表明,样品的预测值与实测值之间无显著性差异。
462 tobacco samples from 5 provinces were chosen for establishing the near infrared models of their NNK contents by near infrared spectrometry( NIS). The external validation was studied by the models. The results showed that the content of NNK in tobacco was significantly correlated with the content of total nitrogen,nicotine,total sugar and reducing sugar. And the average relative errors of predicted values of NNK by the NIS were 6. 88%. The results of T-test showed that there were no significant differences between predicted values and determined values of the samples.
462 tobacco samples from 5 provinces were chosen for establishing the near infrared models of their NNK contents by near infrared spectrometry( NIS). The external validation was studied by the models. The results showed that the content of NNK in tobacco was significantly correlated with the content of total nitrogen,nicotine,total sugar and reducing sugar. And the average relative errors of predicted values of NNK by the NIS were 6. 88%. The results of T-test showed that there were no significant differences between predicted values and determined values of the samples.
引文
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[18]陈霞,金立锋,刘萍萍,等. UPLC-MS/MS法同时测定烟叶中的TSNAs[J].烟草科技,2016,49(3):62-67.
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[20]毛友安,魏新亮,钟科军,等.烟草特有亚硝胺分析方法研究进展[J].烟草科技,2004(7):33-36.
[2]曾凡海,李卫,周冀衡,等.烟草特有亚硝胺(TSNA)的研究进展[J].中国农学通报,2010,26(10):82-86.
[3]刘立全. TSNS释放量与配方叶组成分之间的关系[J].烟草科技,2002(1):29-30.
[4]雷新有,张建斌,白小华,等.烟草特有亚硝胺降解技术研究进展[J].山东化工,2011,40(9):33-36.
[5]李诗平,舒俊生,章存勇,等.亚硝酸盐清除剂阻断烟草特有亚硝胺形成的研究[J].安徽农业科学,2010,38(35):20472-20473.
[6]刘万峰,王元英.烟叶中烟草特有亚硝胺(TSNA)的研究进展[J].中国烟草科学,2002,23(2):11-14.
[7]魏玉玲,宋普球,缪明明.降低烟草特有亚硝胺含量的微波处理方法综述[J].烟草科技,2002(3):18-19.
[8]庞永强,陈再根,侯宏卫,等.抽吸方式对卷烟主流烟气烟草特有亚硝胺释放量的影响[J].烟草科技,2009(2):46-49.
[9]崔韬.气相色谱法分析烟草特有亚硝胺[J].四川烟草通讯,2008,7:28.
[10]张霞,朱东来,韩熠,等. GC-TEA法测定卷烟主流烟气TSNAs前处理方法的改进[J].中国烟草学报,2015,21(3):12-19.
[11]顾文博,周宛虹,陆怡峰,等.气相色谱-三重四极杆串联质谱法测定卷烟烟气中的烟草特有亚硝胺[J].烟草科技,2013,48(10):40-43.
[12]王保兴,汪旭,王玉,等.烤烟中烟草特有亚硝胺的对比[J].烟草科技,2010(11):47-50.
[13] Xia B,Xia Y,Wong J,et al. Quantitative analysis of five tobaccospecific N-nitrosamines in urine by liquid chromatography-atmospheric pressure ionization tandem mass spectrometry[J]. Biomedical Chromatography,2014,28(3):375-384.
[14] Xia Y,Mc Guffey J E,Bhattacharyya S,et al. Analysis of the tobaccospecific nitrosamine 4(-methylnitrosamino)-1-(3-pyridyl)-1-butanol in urine by extraction on a molecularly imprinted polymer column and liquid chromatography/atmospheric pressure ionization tandem mass spectrometry[J]. Anal Chem,2005,77(23):7639-7645.
[15]朱文静,杨俊,刘百战,等. UPLC-MS/M S对卷烟烟气中4种烟草特有亚硝胺的快速测定[J].分析测试学报,2010,29(1):26-30.
[16]李勇,吴名剑,练文柳,等. SPE-LC/MS/MS快速测定卷烟丝中的烟草特有亚硝胺类化合物[J].分析试验室,2007,26(6):65-68.
[17]丁时超,杜文,任建新,等. LC-MS/MS定量分析卷烟中的烟草特有亚硝胺[J].中国烟草学报,2005,11(6):17-22.
[18]陈霞,金立锋,刘萍萍,等. UPLC-MS/MS法同时测定烟叶中的TSNAs[J].烟草科技,2016,49(3):62-67.
[19]唐丽云,丁时超,郭紫明,等.卷烟主流烟气中烟草特有亚硝胺(TSNAs)的LC-MS/MS测定方法研究[J].化学研究与应用,2013(1):111-116
[20]毛友安,魏新亮,钟科军,等.烟草特有亚硝胺分析方法研究进展[J].烟草科技,2004(7):33-36.