理气散结颗粒对大鼠乳腺增生的影响
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摘要
目的研究理气散结颗粒对大鼠乳腺增生的影响。方法 96只大鼠随机分为空白对照组、模型对照组、他莫昔芬组(0. 003 3 g/kg)、小金丸组(0. 200 0 g/kg)、理气散结丸组(1. 957 0 g/kg)及理气散结颗粒低、中、高剂量组(0. 978 5、1. 957 0、3. 914 0 g/kg),每组12只,除空白对照组外各组大鼠肌内注射苯甲酸雌二醇和黄体酮以诱导乳腺增生。给药30 d后取血,ELISA法测定血清雌二醇(E2)、孕酮(P)水平,免疫组化法检测乳腺组织雌激素受体(ER)、孕激素受体(PR)蛋白表达,光镜下观察乳腺组织病理变化。结果与模型对照组比较,理气散结颗粒中剂量组ER蛋白表达显著降低(P<0. 05);高剂量组E2水平显著升高(P<0. 05),ER、PR蛋白表达显著降低(P<0. 05,P<0. 01);低剂量组组织增生明显改善(P<0. 05)。结论理气散结颗粒对大鼠乳腺增生的影响可能与改善乳腺组织中ER、PR蛋白表达有关。
AIM To study the effects of Liqi Sanjie Granules on mammary gland hyperplasia in rats.METHODS Ninety-six rats were randomly divided into blank control group,model control group,tamoxifen group( 0. 003 3 g/kg),Xiaojin Pills group( 0. 200 0 g/kg),Liqi Sanjie Pills group( 1. 957 0 g/kg) and Liqi Sanjie Granules low-dose,medium-dose,high-dose groups( 0. 978 5,1. 957 0,3. 914 0 g/kg),12 rats in each group,the rats in various groups( except for the blank control group) were given intramuscular injection of benzoate estradiol and progesterone to induce mammary gland hyperplasia. Blood collection was made after 30-day administration,serum estradiol( E2) and progesterone( P) levels were determined by ELISA,estrogen receptor( ER)and progesterone receptor( PR) protein expressions in mammary gland tissue were detected by immunohistochemistry,and the pathological changes of mammary gland tissue were observed under light microscope. RESULTS Compared with the model control group,the Liqi Sanjie Granules medium-dose group demonstrated significantly decreased ER protein expression( P<0. 05),the Liqi Sanjie Granules high-dose group exhibited markedly increased E2level( P<0. 05) with obviously reduced ER and PR protein expressions( P<0. 05,P<0. 01),and obviously improved tissue hyperplasia( P<0. 05) was observable in the Liqi Sanjie Granules low-dose group. CONCLUSION The effects of Liqi Sanjie Granules on mammary gland hyperplasia in rats may contribute to improving ER and PR protein expressions.
AIM To study the effects of Liqi Sanjie Granules on mammary gland hyperplasia in rats.METHODS Ninety-six rats were randomly divided into blank control group,model control group,tamoxifen group( 0. 003 3 g/kg),Xiaojin Pills group( 0. 200 0 g/kg),Liqi Sanjie Pills group( 1. 957 0 g/kg) and Liqi Sanjie Granules low-dose,medium-dose,high-dose groups( 0. 978 5,1. 957 0,3. 914 0 g/kg),12 rats in each group,the rats in various groups( except for the blank control group) were given intramuscular injection of benzoate estradiol and progesterone to induce mammary gland hyperplasia. Blood collection was made after 30-day administration,serum estradiol( E2) and progesterone( P) levels were determined by ELISA,estrogen receptor( ER)and progesterone receptor( PR) protein expressions in mammary gland tissue were detected by immunohistochemistry,and the pathological changes of mammary gland tissue were observed under light microscope. RESULTS Compared with the model control group,the Liqi Sanjie Granules medium-dose group demonstrated significantly decreased ER protein expression( P<0. 05),the Liqi Sanjie Granules high-dose group exhibited markedly increased E2level( P<0. 05) with obviously reduced ER and PR protein expressions( P<0. 05,P<0. 01),and obviously improved tissue hyperplasia( P<0. 05) was observable in the Liqi Sanjie Granules low-dose group. CONCLUSION The effects of Liqi Sanjie Granules on mammary gland hyperplasia in rats may contribute to improving ER and PR protein expressions.
引文
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[8]林妮,邱玉文,何贡云,等.荔枝核皂苷对大鼠乳腺增生的作用及对雌激素信号通路的影响[J].中药材,2015,38(4):798-802.
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[11]林妮,吕俊华,潘竞锵,等.荔枝核提取物抑制乳腺增生模型大鼠乳腺组织ER和PR表达及促进乳腺细胞凋亡作用研究[J].中国药房,2010,21(11):975-977.
[12]段好刚,魏玉辉,李波霞,等.疏乳消块丸对大鼠乳腺增生的治疗作用及其机制[J].中国医院药学杂志,2012,32(16):1273-1276.
[13] Lucas J N,Rudmann D G,Credille K M,et al. The rat mammary gland:morphologic changes as an indicator of systemic hormonal perturbations induced by xenobiotics[J]. Toxicol Pathol,2007,35(2):199-207.
[14] Drukker B H. Fibrocystic change of the breast[J]. Clin Obstet Gynecol,1994,37(4):903-915.
[15]周朝晖,刘鸿雁,安晓芳,等.安乳消痛丸对乳腺增生模型大鼠的影响[J].西部中医药,2016,29(4):26-29.
[16] Thakkar J P,Mehta D G. A review of an unfavorable subset of breast cancer:estrogen receptor positive progesterone receptor negative[J]. Oncologist,2011,16(3):276-285.
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[18]王忠超,李敏,张彬,等.蒙药乳腺-Ⅰ号对乳腺增生大鼠抗氧化能力、乳腺组织雌激素受体和孕激素受体表达的影响[J].吉林大学学报(医学版),2012,38(6):1052-1057,1036.
[19]车艺,厉红元. Cyr61的分子病理及与乳腺癌的关系[J].实用临床医药杂志,2009,13(15):15-16,20.
[20]赵玉斌,张少华,杨京华. EphrinA1、ERα和ERβ在乳腺癌中的表达及其意义[J].现代肿瘤医学,2014,22(4):833-836.
[21]周翠娥.以中药为主预防并治愈乳腺癌[J].实用医技杂志,2010,17(5):439-440.
[22]王勇,陈超.归参胶囊对乳腺增生病大鼠激素水平的影响[J].中国临床康复,2005,9(14):1622-1631.
[2]王雄,吴金虎,陈永刚,等.回乳抑增Ⅰ号对实验性乳腺增生大鼠的作用及其机制研究[J].中国实验方剂学杂志,2013,19(13):274-279.
[3]王勇,陈超.归参胶囊对大鼠乳腺增生及脾脏T淋巴细胞亚群的影响[J].中成药,2005,27(11):1297-1301.
[4]乔丽莉.红金消结胶囊联合他莫昔芬治疗绝经后妇女乳腺增生症的疗效观察[J].中国药房, 2013,24(16):1502-1504.
[5]周会,龙子江,陈明,等.抗乳增片对乳腺增生模型大鼠乳腺组织的影响[J].中成药,2008,30(10):1536-1538.
[6]谷丽艳,解静茹,刘齐,等.解毒胶囊对乳腺增生大鼠VEGF和b FGF表达的影响[J].中华中医药学刊,2016,34(7):1559-1562.
[7]吴晓燕,瞿发林,许立.乳康口服液对乳腺增生大鼠作用机制研究[J].中国药师,2016,19(4):647-651.
[8]林妮,邱玉文,何贡云,等.荔枝核皂苷对大鼠乳腺增生的作用及对雌激素信号通路的影响[J].中药材,2015,38(4):798-802.
[9]顾丰华,陈嘉,刘翔,等.鳖甲煎丸治疗乳腺增生的药效研究[J].世界临床药物,2015,36(2):90-95.
[10]胡建平,李珊珊,刘元新.生鸡内金对乳腺增生病大鼠的作用研究[J].实用中西医结合临床,2015,15(12):81-83.
[11]林妮,吕俊华,潘竞锵,等.荔枝核提取物抑制乳腺增生模型大鼠乳腺组织ER和PR表达及促进乳腺细胞凋亡作用研究[J].中国药房,2010,21(11):975-977.
[12]段好刚,魏玉辉,李波霞,等.疏乳消块丸对大鼠乳腺增生的治疗作用及其机制[J].中国医院药学杂志,2012,32(16):1273-1276.
[13] Lucas J N,Rudmann D G,Credille K M,et al. The rat mammary gland:morphologic changes as an indicator of systemic hormonal perturbations induced by xenobiotics[J]. Toxicol Pathol,2007,35(2):199-207.
[14] Drukker B H. Fibrocystic change of the breast[J]. Clin Obstet Gynecol,1994,37(4):903-915.
[15]周朝晖,刘鸿雁,安晓芳,等.安乳消痛丸对乳腺增生模型大鼠的影响[J].西部中医药,2016,29(4):26-29.
[16] Thakkar J P,Mehta D G. A review of an unfavorable subset of breast cancer:estrogen receptor positive progesterone receptor negative[J]. Oncologist,2011,16(3):276-285.
[17]杨素清,王玉玺.消核止痛饮对乳腺增生病性激素调节作用的临床研究[J].中医药信息,2004,21(2):52-54.
[18]王忠超,李敏,张彬,等.蒙药乳腺-Ⅰ号对乳腺增生大鼠抗氧化能力、乳腺组织雌激素受体和孕激素受体表达的影响[J].吉林大学学报(医学版),2012,38(6):1052-1057,1036.
[19]车艺,厉红元. Cyr61的分子病理及与乳腺癌的关系[J].实用临床医药杂志,2009,13(15):15-16,20.
[20]赵玉斌,张少华,杨京华. EphrinA1、ERα和ERβ在乳腺癌中的表达及其意义[J].现代肿瘤医学,2014,22(4):833-836.
[21]周翠娥.以中药为主预防并治愈乳腺癌[J].实用医技杂志,2010,17(5):439-440.
[22]王勇,陈超.归参胶囊对乳腺增生病大鼠激素水平的影响[J].中国临床康复,2005,9(14):1622-1631.