侗药血马鞭水提物对吡啶诱导的急性非感染咽喉炎大鼠的保护作用研究
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摘要
[目的]在吡啶所致的急性非感染性咽炎大鼠模型上初步评价侗药血马鞭水提取物对咽喉炎的保护作用。[方法] SD大鼠被随机分为5组,包括吡啶+侗药血马鞭水提物低、高剂量组(500、1 000 mg/kg,灌胃给药)、吡啶+地塞米松组(4 mg/kg,灌胃给药)、正常对照组、模型对照组。造模前,各组大鼠尾静脉注射无菌伊文思蓝溶液(EB),随后大鼠通过采用10%吡啶溶液涂抹咽喉部建立咽喉炎模型,正常对照组涂抹相同体积的无菌生理盐水。造模1 h后处死大鼠,观察大鼠咽部并拍照,同时对咽部组织EB渗出量进行测定。称量各组大鼠主要脏器质量,采用苏木精-伊红(HE)染色法观察各组大鼠咽喉组织病理改变情况。[结果]与正常组大鼠相比,模型组大鼠咽喉组织EB渗出显著增加、炎性细胞浸润明显增多、黏液腺细胞排列分散不规则且部分肥大破裂,并伴有组织出血。与模型组比较,侗药血马鞭水提物高剂量组大鼠和地塞米松组大鼠EB渗出明显降低(P<0.05),组织炎性细胞浸润显著减少。[结论]侗药血马鞭水提物对吡啶所诱导的急性非感染性咽炎大鼠有很好的保护作用。
[Objective] To investigate the protective effects of the water extract of Callicarpa kwangtungensis Chun on acute noninfectious rat pharyngitis induced by pyridine. [Methods] SD rats were randomly divided into five groups, which are include low and high dose of the water extract of Callicarpa kwangtungensis Chun and pyridine groups(500, 1 000 mg/kg, ig), dexamethasone and pyridine group(4 mg/kg, ig), normal control group and pyridine model group. SD rats were injected with Evans Blue via tail vein, followed rats were smeared with 10 % pyridine in the pharynx. The normal control group rats were smeared with the same volume of sterile saline water. After 1 h, these rats were sacrificed, the pharyngeal tissues of rats were photographed and isolated for the detection of EB exudation. The main organs of rats were separated and weighed. The hematoxylin-eosin staining method was used to observe the pathological changes of the pharyngeal tissues of rats. [Results] Compared with the normal control group rats, the pyridine model rats shown several pathological characteristics, a significantly increase of EB exudation and the infiltration of inflammatory cells was observed in the pharyngeal tissues, as well as the irregular arrangement, hypertrophy and rupture of the mucous gland cells were also obtained in the pharyngeal tissues, which was accompanied with bleeding. Compared with the pyridine model group rats, an obviously decrease of EB exudation and the infiltration of inflammatory cells was observed in the pharyngeal tissues of experimental drugs group rats and the positive control dexamethasone group rats. [Conclusion] The water extract of Callicarpa kwangtungensis Chun has a protective effect on acute non-infectious pharyngitis induced by pyridine.
[Objective] To investigate the protective effects of the water extract of Callicarpa kwangtungensis Chun on acute noninfectious rat pharyngitis induced by pyridine. [Methods] SD rats were randomly divided into five groups, which are include low and high dose of the water extract of Callicarpa kwangtungensis Chun and pyridine groups(500, 1 000 mg/kg, ig), dexamethasone and pyridine group(4 mg/kg, ig), normal control group and pyridine model group. SD rats were injected with Evans Blue via tail vein, followed rats were smeared with 10 % pyridine in the pharynx. The normal control group rats were smeared with the same volume of sterile saline water. After 1 h, these rats were sacrificed, the pharyngeal tissues of rats were photographed and isolated for the detection of EB exudation. The main organs of rats were separated and weighed. The hematoxylin-eosin staining method was used to observe the pathological changes of the pharyngeal tissues of rats. [Results] Compared with the normal control group rats, the pyridine model rats shown several pathological characteristics, a significantly increase of EB exudation and the infiltration of inflammatory cells was observed in the pharyngeal tissues, as well as the irregular arrangement, hypertrophy and rupture of the mucous gland cells were also obtained in the pharyngeal tissues, which was accompanied with bleeding. Compared with the pyridine model group rats, an obviously decrease of EB exudation and the infiltration of inflammatory cells was observed in the pharyngeal tissues of experimental drugs group rats and the positive control dexamethasone group rats. [Conclusion] The water extract of Callicarpa kwangtungensis Chun has a protective effect on acute non-infectious pharyngitis induced by pyridine.
引文
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[13]Uemura J,Nagpal R,Zerbinati N,et al.Effect of VBC-1814/7J,a poly-phytocompound,on a non-infectious model of pharyngitis[J].Experimental and therapeutic medicine,2017,13:3075-3080.
[14]Viswanatha GL,Thippeswamy AH,Thippeswamy HC,et al.Ameliorative effect of Koflet formulations against pyridine-induced pharyngitis in rats[J].Toxicology Reports,2014(1):293-299.
[15]Li Y,Zheng B,Tian H,et al.Yupingfeng Powder relieves the immune suppression induced by dexamethasone in mice[J].Journal of ethnopharmacology,2017(200):117-123.
[2]梁彦,丁莎.慢性咽炎试试整体点穴[J].中医健康养生,2017,3(2):55-55.
[3]Somro A,Akram M,Khan M I,et al.Pharyngitis and sore throat:Areview[J].African Journal of Biotechnology,2011,10(33):6190-6197.
[4]梁璐,朴晋华,马建丽.急性和慢性咽炎的发病机制与动物模型建立的研究现状[J].临床医药实践,2012,21(7):540-542.
[5]Di MF,Barucco M,Guerriero F.Diagnosis and treatment of acute pharyngitis/tonsillitis:a preliminary observational study in General Medicine[J].European Review for Medical and Pharmacological Sciences,2016,20(23):4950-4954.
[6]Kannan I,Edwin B,Prasanna V,et al.Aetiology and the use of antibiotics in the case of acute pharyngitis:A review[J].International Journal of Pharmaceutical&Clinical Research,2015,7(4):226-230.
[7]Korb K,Scherer M,Chenot J F.Steroids as adjuvant therapy for acute pharyngitis in ambulatory patients:a systematic review[J].Annals of Family Medicine,2010,8(1):58-63.
[8]国家药典委员会.中华人民共和国药典[M].北京:中国医药科技出版社,2015.
[9]陈冈,付辉政,戴冕,等.侗药血马鞭茎和叶乙醇水提取物的乙酸乙酯溶性部位化学成分研究(Ⅱ)[J].中国现代中药,2017,19(8):1117-1119.
[10]聂韡,朱培林,黄丽莉,等.侗药血马鞭药材的研究进展[J].中国现代中药,2011,13(9):37-39.
[11]封银曼,王停,高志卿,等.急性咽炎动物模型研究[J].中国实验方剂学杂志,2001,7(3):52.
[12]Viswanatha GL,Thippeswamy AH,Rafiq M,et al.Novel experimental model of non-infectious pharyngitis in rats[J].Journal of pharmacological and toxicological methods,2014,69(2):189-195.
[13]Uemura J,Nagpal R,Zerbinati N,et al.Effect of VBC-1814/7J,a poly-phytocompound,on a non-infectious model of pharyngitis[J].Experimental and therapeutic medicine,2017,13:3075-3080.
[14]Viswanatha GL,Thippeswamy AH,Thippeswamy HC,et al.Ameliorative effect of Koflet formulations against pyridine-induced pharyngitis in rats[J].Toxicology Reports,2014(1):293-299.
[15]Li Y,Zheng B,Tian H,et al.Yupingfeng Powder relieves the immune suppression induced by dexamethasone in mice[J].Journal of ethnopharmacology,2017(200):117-123.
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