麻黄定喘汤和鹿茸大补汤的急性毒性研究
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摘要
目的:研究麻黄定喘汤和鹿茸大补汤对昆明小鼠灌胃的急性毒性,并评价其安全性。方法:选取昆明鼠种50只,雄雌各半,灌胃给药。通过预实验结果,半数致死量(LD50)足够大,无明确值,采用临床成人药量的100倍,麻黄定喘汤给药组采用23 000 mg·kg~(-1),鹿茸大补汤给药组采用26 000 mg·kg~(-1),并设置生理盐水对照组,按照小鼠体质量10 ml·kg~(-1)的剂量灌胃给药,连续观察7日,并记录小鼠体态、精神状况、体质量、摄食量等。观察7日后,用颈椎脱臼法将小鼠处死,解剖观察,取脑、肝、脾、肺、肾脏器称重,计算脏器系数。对小鼠组织进行生化检查,计算谷草转氨酶活力(U/gprot)和谷丙转氨酶活力(U/gprot)。将麻黄定喘汤给药组和鹿茸大补汤给药组所得数据与生理盐水对照组进行比较。结果:经口急性毒性实验未见动物有中毒症状,无动物死亡,外观表现正常。通过统计处理,所得数据与对照组比较,无统计学意义(P>0.05)。结论:麻黄定喘汤和鹿茸大补汤安全性较高,临床中可以适量使用。
Objective: To study the acute toxicity of Mahuang Dingchuan Decoction and Lurong Dabu Decoction in mice to evaluate their safety. Methods: 50 Kunming mice were given medications through gavage administration. LD_(50) was too large to caculate in the preliminary experiment. 100 times dose of clinical adult dosage was applied to mice,i. e. for Mahuang Dingchuan Decoction,it was 23 000 mg·kg~(-1); for Lurong Dabu Decoction,it was 26 000 mg·kg~(-1); the control group was given normal saline with dose of 10 ml·kg~(-1). Body status,mental state,body mass,and food intake were observed in mice for continuous 7 days. After 7 days of observation,the mice were sacrificed by cervical dislocation method. Samples of brain,liver,spleen,lung,and kidney were weighed to calculate organ coefficient. The tissues of mice were examined by biochemical detection to measure the activities of glutamic-oxalacetic transaminase and glutamic-pyruvic transaminase. The data were compared in the three groups. Results: The acute toxicity test showed no symptoms of poisoning,no animal death,and the mice showed normal appearance. There were no significant differences among three groups( P > 0. 05). Conclusion: Mahuang Dingchuan Decoction and Lurong Dabu Decoction have high safety,which can be both applied in clinic with right amount.
Objective: To study the acute toxicity of Mahuang Dingchuan Decoction and Lurong Dabu Decoction in mice to evaluate their safety. Methods: 50 Kunming mice were given medications through gavage administration. LD_(50) was too large to caculate in the preliminary experiment. 100 times dose of clinical adult dosage was applied to mice,i. e. for Mahuang Dingchuan Decoction,it was 23 000 mg·kg~(-1); for Lurong Dabu Decoction,it was 26 000 mg·kg~(-1); the control group was given normal saline with dose of 10 ml·kg~(-1). Body status,mental state,body mass,and food intake were observed in mice for continuous 7 days. After 7 days of observation,the mice were sacrificed by cervical dislocation method. Samples of brain,liver,spleen,lung,and kidney were weighed to calculate organ coefficient. The tissues of mice were examined by biochemical detection to measure the activities of glutamic-oxalacetic transaminase and glutamic-pyruvic transaminase. The data were compared in the three groups. Results: The acute toxicity test showed no symptoms of poisoning,no animal death,and the mice showed normal appearance. There were no significant differences among three groups( P > 0. 05). Conclusion: Mahuang Dingchuan Decoction and Lurong Dabu Decoction have high safety,which can be both applied in clinic with right amount.
引文
[1]张梦婷,张嘉丽,任阳阳.麻黄的研究进展[J].世界中医药,2016,11(9):1917-1921.
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[11]杨旭,王琦越,付知勤.外用制剂芎桃二乌止痛酊急性毒性实验研究[J].辽宁中医药大学学报,2017,19(1):58-60.
[12]张秀梅,袁海鹰,李桂华.中药与中医的关系以及中药在现代医学中的地位[J].求医问药,2013,11(2):328-329.
[13]刘世杰,魏文静,胡书平.朝医药与中医药的比较[J].中央民族大学学报(自然科学版),2012,21(4):56-61.
[14]叶吉明,龚汉明.中药毒性的探讨[J].中国实用医药,2011,6(1):249-251.
[15]李京玉,冯晓纯,郑明昱.朝医加味麻黄定喘汤在小鼠哮喘模型中对ERK信号通路的影响[J].中华中医药杂志,2013,28(12):3744-3747.
[16]金燕,杨今实,郑明昱.朝医鹿茸大补汤对哮喘小鼠气道炎性反应的影响[J].中华中医药杂志,2016,31(9):3490-3493.
[2]谭晓梅,郭阳,余林中.麻黄-杏仁药对配伍及不同配比对急性毒性及平喘作用的影响[J].中药药理与临床,2013,29(1):82-84.
[3]赵岩,妙志岩,郜玉钢.人参茸芝胶囊的急性毒性实验研究[J].食品安全质量检测学报,2015,6(2):614-618.
[4]隋海霞,高芃,耿桂英.鹿茸人参制剂的毒理学安全性评价[J].现代预防医学,2007,34(23):4448-4449.
[5]肖经纬,李斌,张星.急性经口毒性评价试验方法的研究进展[J].国外医学(卫生学分册),2007,34(2):84-88.
[6]林伊莉,陈丹,曾华平.百花含片的急性毒性试验[J].福建中医药,2016,47(1):24-25.
[7]朱智德,蒋林,罗宇东.六味葛蓝降脂片对小鼠急性经口毒性及遗传毒性的试验研究[J].中国当代医药,2016,23(12):4-7.
[8]于葆华,丁晓云,盛国荣.退银汤急性毒性试验[J].中医药导报,2016,22(1):43-45.
[9]古丽娜·巴克,李海英.松萝酸对小鼠急性毒性试验研究[J].饲料研究,2016,39(7):51-53.
[10]杜正彩,郝二伟,黄庆.复方绞股蓝益智颗粒急性毒性与慢性毒性实验研究[J].当代医学,2012,18(19):1-3.
[11]杨旭,王琦越,付知勤.外用制剂芎桃二乌止痛酊急性毒性实验研究[J].辽宁中医药大学学报,2017,19(1):58-60.
[12]张秀梅,袁海鹰,李桂华.中药与中医的关系以及中药在现代医学中的地位[J].求医问药,2013,11(2):328-329.
[13]刘世杰,魏文静,胡书平.朝医药与中医药的比较[J].中央民族大学学报(自然科学版),2012,21(4):56-61.
[14]叶吉明,龚汉明.中药毒性的探讨[J].中国实用医药,2011,6(1):249-251.
[15]李京玉,冯晓纯,郑明昱.朝医加味麻黄定喘汤在小鼠哮喘模型中对ERK信号通路的影响[J].中华中医药杂志,2013,28(12):3744-3747.
[16]金燕,杨今实,郑明昱.朝医鹿茸大补汤对哮喘小鼠气道炎性反应的影响[J].中华中医药杂志,2016,31(9):3490-3493.
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