青海省6种高寒禾本科牧草的耐盐性
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摘要
本研究通过对6种禾本科高寒牧草同德贫花鹅观草(Roegneria pauciflora Tongde)、草原看麦娘(Alopecuruspratensis)、大颖草(Roegneria grandiglumis)、无芒雀麦(Bromus inermis)、扁穗冰草(Agropyron cristatum)和同德短芒披碱草(Elymus breviaristatus Tongde)萌发期及幼苗期分别进行不同浓度的NaCl溶液处理,测定其萌发期的发芽率和幼苗期的脯氨酸含量、叶绿素含量、丙二醛(MDA)含量、可溶性糖含量以及游离脯氨酸(PRO)含量。结果表明,随着NaCl浓度的增加,6种禾本科牧草叶片中的脯氨酸含量和MDA含量随之增加,而叶绿素和可溶性糖含量呈先升后降的变化。运用隶属函数对6种禾本科牧草萌发期及幼苗期的耐盐性进行综合评价分析。6种禾本科牧草的耐盐性依次为无芒雀麦>草原看麦娘>大颖草>同德短芒披碱草>扁穗冰草>同德贫花鹅观草。
Stress caused by different NaCl concentrations, germination proportion chlorophyll content, proline content, MDA content, and soluble sugar content were measured during the germination and seedling period of six grass species. As NaCl concentrations increased, proline content and MDA content in leaves of all six grass species increased. Chlorophyll and soluble sugar content first decreased and then increased as NaCl concentrations increased. The membership function was used to evaluate the antisalinity of six grass species in germination and seedling periods. Results revealed the following order of salinity tolerance for the six grasses(from largest to smallest): Bromus inermis > Alopecurus pratensis > Roegneria grandiglumis > Elymus breviaristatus Tongde > Agropyron cristatum > Roegneria pauciflora Tongde.
Stress caused by different NaCl concentrations, germination proportion chlorophyll content, proline content, MDA content, and soluble sugar content were measured during the germination and seedling period of six grass species. As NaCl concentrations increased, proline content and MDA content in leaves of all six grass species increased. Chlorophyll and soluble sugar content first decreased and then increased as NaCl concentrations increased. The membership function was used to evaluate the antisalinity of six grass species in germination and seedling periods. Results revealed the following order of salinity tolerance for the six grasses(from largest to smallest): Bromus inermis > Alopecurus pratensis > Roegneria grandiglumis > Elymus breviaristatus Tongde > Agropyron cristatum > Roegneria pauciflora Tongde.
引文
[1]VEERANAGAMALLAIAH G,CHANDRAOBULREDDY P,JYOTHSNAKUMARI G,SUDHAKAR C.Glutamine synthetase expression and pyrrotine-5-carboxylate reductase activity influence proline accumulation in two cultivars of foxtail millet(Setaria italica L.)with differential salt sensitivity.Environmental and Experimental Botany,2007,60:239-244.
[2]BARTELS D,SUNKAR R.Drought and salt tolerance in plants.Critical Reviews in Plant Sciences,2005,24:23-58.
[3]乔枫,罗桂花,耿贵工.蚕豆幼苗对NaCI和NaHCO3胁迫的生理响应.安徽农业大学学报,2011,38(5):783-787.QIAO F,LUO G H,GEN G G.Physiological responses of vicia faba seedlings to NaCl and NaHCO3 stress.Journal of Anhui Agricultural University,2011,38(5):783-787.
[4]MELONI D A,OLIVA M A,MARTINEZ C A.Photosynthesis and activity of superoxide dismutase,peroxidese and glutathione reductase in cotton under salt stress.Environmental and Experimental Botany,2003,49:69-76.
[5]陈惠哲,NATALIA LADATKO,朱德峰,林贤青,张玉屏,孙宗修.盐胁迫下水稻苗期Na+和K+吸收与分配规律的初步研究.植物生态学报,2007,31(5):937-945.CHEN H Z,NATALIA L,ZHU D F,LIN X Q,ZHANG Y P,SUN Z X.Absorption and diatribution of Na+and K+in rice seedline under salt stress.Journal of Plant Ecology,2007,31(5):937-945.
[6]乔旭,黄爱军,褚贵新.植物对盐分胁迫的响应及其耐盐机理研究进展.新疆农业科学,2011,48(11):2089-2094.QIAO X,HUANG A J,CHU G X.Research progress in the effects of salt stress on plant and the mechanism of plant resistance.Agriculture Science of Xinjiang,2011,48(11):2089-2094.
[7]沈艳,谢应忠.牧草抗旱性和耐盐性研究进展.宁夏农学院学报,2004,25(1):65-69.SHEN Y,XIE Y Z.The progress of studies on forage grass drought resistance and salt tolerance.Journal of Ningxia Agricultural College,2004,25(1):65-69.
[8]余玲,王彦荣,孙建华.野大麦种子萌发条件及抗逆性研究.草业学报,1999,8(1):50-57.YU L,WANG Y R,SUN J H.Studies on germination condition and stress resistance of Hordeum brevisubulatum seeds.Journal of Grass Industry,1999,8(1):50-57.
[9]毛培春,王勇.不同禾本科牧草材料种子萌发的耐盐性试验.内蒙古农业大学学报(自然科学版),2004(2):115-118.MAO P C,WANG Y.Experiment on salt resistance in seed germination stage of six grass varieties.Journal of Inner Mongolia Agricultural University(Natural Science Edition),2004(2):115-118.
[10]王晓龙,李红,闫利军,米福贵,于洁,贾振宇,杨曌,杨伟光.5种禾本科牧草种子萌发及幼苗耐盐性鉴定.种子,2016(8):27-31.WANG X L,LI H,YAN L J,MI F G,YU J,JIA Z Y,YANG Z,YANG W G.Salt-tolerance of seed germination and seedling growth for five grass species.Seeds,2016(8):27-31.
[11]李京蓉,周学斌,马真,刘泽华,石国玺,王文颖,张中华,郭美玲,姚步青,张春辉,马丽,周华坤.6种高寒牧区禾本科牧草抗旱性研究与评价.草地学报,2018,26(3):659-665.LI J R,ZHOU X B,MA Z,LIU Z H,SHI G X,WANG W Y,ZHANG Z H,GUO M L,YAO B Q,ZHANG C C,MA L,ZHOU HK.Research and evalution on drought resistance of six grasses in high-cold pastoral area.Acta Agrestia Sinica,2018,26(3):659-665.
[12]朱教君,李智辉,康宏樟,范业展.聚乙二醇模拟水分胁迫对沙地樟子松种子萌发影响研究.应用生态学报,2005,16(5):801-804.ZHU J J,LI Z H,KANG H Z,FAN Y Z.Effects of polyethylene glycol(PEG)-simulated drought stress on Pinus sylvestris var.Mongolica seed germination on sandy land.Journal of Applied Ecology,2005,16(5):801-804.
[13]施积炎,丁贵杰.水分胁迫对不同种源马尾松种子发芽的影响.山地农业生物学报,2000,19(5):32-337.SHI J Y,DING G J.The effect of water stress on germination of Masson Pine seeds from different provenance.Journal of Mountain Agricultural Biology,2000,19(5):32-337.
[14]冯淑华,陈雅君.干旱对草地早熟禾种子萌发的影响.草地与草坪,2006(1):70-71.FENG S H,CHEN Y J.The influence of drought on seeds'germination ability of Poa pratensis cultivars.Grassland and Lawn,2006(1):70-71.
[15]张志良,瞿伟菁.植物生理学实验指导.3版.北京:高等教育出版社,2005.ZHANG Z L,QU W J.Plant Physiology Experiment Guidance.3rd Edition.Beijing:Higher Education Press,2005.
[16]武永军,何国强,史艳茹,梁宗锁.不同pH值缓冲液处理下蚕豆叶片相对含水量、脯氨酸及丙二醛含量的变化.干旱地区农业研究,2009,27(6):169-172.WU Y J,HE G Q,SHI Y R,LIANG Z S.The relative water content,proline and malondialdehyde content of broad bean leaves were treated with different pH buffer.Agricultural Research in Arid Regions,2009,27(6):169-172.
[17]罗志娜,赵桂琴,刘欢.24个燕麦品种种子萌发耐盐性综合评价.草原与草坪,2012,32(1):34-38.LUO Z N,ZHAO G Q,LIU H.24 oat varieties of seed germination of salt resistance comprehensive evaluation.Grassland and Lawn,2012,32(1):34-38.
[18]王晓龙.五种禾本科牧草生物学特性、农艺性状及抗逆性研究.呼和浩特:内蒙古农业大学硕士学位论文,2014.WANG X L.Evaluation of the biologic traits,agronomic characters and stress tolerance for five forage grasses.Master Thesis.Hohhot:Inner Mongolia Agricultural University,2014.
[19]武俊英,刘景辉,翟利剑,李倩,李立军.不同品种燕麦种子萌发和幼苗生长的耐盐性.生态学杂志,2009,28(10):1960-1965.WU J Y,LIU J H,ZHAI L J,LI Q,LI L J.Salt-tolerance of seed germination and seedling growth of different oat varieties.Ecology Journal,2009,28(10):1960-1965.
[20]史燕山,骆建霞,黄家珍,叶军,魏巍.盐胁迫对7种草本地被植物种子萌发的影响.天津农学院学报,2007,14(4):1-4.SHI Y S,LUO J X,HUANG J Z,YE J,WEI E.Effects of salt stress on seed germination of seven herb ground cover plants.Journal of Tianjin Agricultural College,2007,14(4):1-4.
[21]LI M,WANG G X.Effect of drought Stress on activities of cell defense enzymes and lipid peroxidation in glycyrrhiza uralensis seedlings.Acta Ecoloigica Sinica,2002,22(4):503-507.
[22]陈莎莎,姚世响,袁军文,兰海燕.盐生植物灰绿藜对NaCl和NaHCO3胁迫的生理响应.新疆农业科学,2010,47(5):882-887.CHEN S S,YAO S X,YUAN J W,LAN H Y.Physiological responses of halophyte Chenopodium glaucum L.to NaCl and NaHCO3 stresses.Xinjiang Agricultural Sciences,2010,47(5):882-887.
[23]邓敏捷,张晓申,范国强,赵振利,董焱鹏,魏振.四倍体泡桐对盐胁迫生理响应的差异.中南林业科技大学学报,2013,33(11):42-46.DENG M J,ZHANG X S,FAN G Q,ZHAO Z L,DONG Y P,WEI Z.Comparative studies on physiological responses to salt stress in tetraploid paulownia plants.Journal of Central South University of Forestry and Technology,2013,33(11):42-46.
[24]洪森荣,尹明华.红芽芋驯化苗对盐胁迫的光合及生理响应.西北植物学报,2013,33(12):2499-2506.HONG S R,YIN M H.Photosynthetic and physiological responses of red bud taro transplantating seedlings under salt stress.Journal of Northwest Plants,2013,33(12):2499-2506.
[25]廖岩,陈桂珠.三种红树植物对盐胁迫的生理适应.生态学报,2007,27(6):2208-2214.LIAO Y,CHEN G Z.Research on physiological adaptability of three mangrove species to salt stress.Journal of Ecology,2007,27(6):2208-2214.
[26]张丽.平欧杂种榛抗盐碱生理机制研究及其耐盐性评价.北京:中国林业科学研究院,2015.ZHANG L.Study on the Physiological Mechanism of the Hybrid Hazel and Salinity Tolerance.Beijing:Chinese Academy of Forestry Sciences,2015.
[2]BARTELS D,SUNKAR R.Drought and salt tolerance in plants.Critical Reviews in Plant Sciences,2005,24:23-58.
[3]乔枫,罗桂花,耿贵工.蚕豆幼苗对NaCI和NaHCO3胁迫的生理响应.安徽农业大学学报,2011,38(5):783-787.QIAO F,LUO G H,GEN G G.Physiological responses of vicia faba seedlings to NaCl and NaHCO3 stress.Journal of Anhui Agricultural University,2011,38(5):783-787.
[4]MELONI D A,OLIVA M A,MARTINEZ C A.Photosynthesis and activity of superoxide dismutase,peroxidese and glutathione reductase in cotton under salt stress.Environmental and Experimental Botany,2003,49:69-76.
[5]陈惠哲,NATALIA LADATKO,朱德峰,林贤青,张玉屏,孙宗修.盐胁迫下水稻苗期Na+和K+吸收与分配规律的初步研究.植物生态学报,2007,31(5):937-945.CHEN H Z,NATALIA L,ZHU D F,LIN X Q,ZHANG Y P,SUN Z X.Absorption and diatribution of Na+and K+in rice seedline under salt stress.Journal of Plant Ecology,2007,31(5):937-945.
[6]乔旭,黄爱军,褚贵新.植物对盐分胁迫的响应及其耐盐机理研究进展.新疆农业科学,2011,48(11):2089-2094.QIAO X,HUANG A J,CHU G X.Research progress in the effects of salt stress on plant and the mechanism of plant resistance.Agriculture Science of Xinjiang,2011,48(11):2089-2094.
[7]沈艳,谢应忠.牧草抗旱性和耐盐性研究进展.宁夏农学院学报,2004,25(1):65-69.SHEN Y,XIE Y Z.The progress of studies on forage grass drought resistance and salt tolerance.Journal of Ningxia Agricultural College,2004,25(1):65-69.
[8]余玲,王彦荣,孙建华.野大麦种子萌发条件及抗逆性研究.草业学报,1999,8(1):50-57.YU L,WANG Y R,SUN J H.Studies on germination condition and stress resistance of Hordeum brevisubulatum seeds.Journal of Grass Industry,1999,8(1):50-57.
[9]毛培春,王勇.不同禾本科牧草材料种子萌发的耐盐性试验.内蒙古农业大学学报(自然科学版),2004(2):115-118.MAO P C,WANG Y.Experiment on salt resistance in seed germination stage of six grass varieties.Journal of Inner Mongolia Agricultural University(Natural Science Edition),2004(2):115-118.
[10]王晓龙,李红,闫利军,米福贵,于洁,贾振宇,杨曌,杨伟光.5种禾本科牧草种子萌发及幼苗耐盐性鉴定.种子,2016(8):27-31.WANG X L,LI H,YAN L J,MI F G,YU J,JIA Z Y,YANG Z,YANG W G.Salt-tolerance of seed germination and seedling growth for five grass species.Seeds,2016(8):27-31.
[11]李京蓉,周学斌,马真,刘泽华,石国玺,王文颖,张中华,郭美玲,姚步青,张春辉,马丽,周华坤.6种高寒牧区禾本科牧草抗旱性研究与评价.草地学报,2018,26(3):659-665.LI J R,ZHOU X B,MA Z,LIU Z H,SHI G X,WANG W Y,ZHANG Z H,GUO M L,YAO B Q,ZHANG C C,MA L,ZHOU HK.Research and evalution on drought resistance of six grasses in high-cold pastoral area.Acta Agrestia Sinica,2018,26(3):659-665.
[12]朱教君,李智辉,康宏樟,范业展.聚乙二醇模拟水分胁迫对沙地樟子松种子萌发影响研究.应用生态学报,2005,16(5):801-804.ZHU J J,LI Z H,KANG H Z,FAN Y Z.Effects of polyethylene glycol(PEG)-simulated drought stress on Pinus sylvestris var.Mongolica seed germination on sandy land.Journal of Applied Ecology,2005,16(5):801-804.
[13]施积炎,丁贵杰.水分胁迫对不同种源马尾松种子发芽的影响.山地农业生物学报,2000,19(5):32-337.SHI J Y,DING G J.The effect of water stress on germination of Masson Pine seeds from different provenance.Journal of Mountain Agricultural Biology,2000,19(5):32-337.
[14]冯淑华,陈雅君.干旱对草地早熟禾种子萌发的影响.草地与草坪,2006(1):70-71.FENG S H,CHEN Y J.The influence of drought on seeds'germination ability of Poa pratensis cultivars.Grassland and Lawn,2006(1):70-71.
[15]张志良,瞿伟菁.植物生理学实验指导.3版.北京:高等教育出版社,2005.ZHANG Z L,QU W J.Plant Physiology Experiment Guidance.3rd Edition.Beijing:Higher Education Press,2005.
[16]武永军,何国强,史艳茹,梁宗锁.不同pH值缓冲液处理下蚕豆叶片相对含水量、脯氨酸及丙二醛含量的变化.干旱地区农业研究,2009,27(6):169-172.WU Y J,HE G Q,SHI Y R,LIANG Z S.The relative water content,proline and malondialdehyde content of broad bean leaves were treated with different pH buffer.Agricultural Research in Arid Regions,2009,27(6):169-172.
[17]罗志娜,赵桂琴,刘欢.24个燕麦品种种子萌发耐盐性综合评价.草原与草坪,2012,32(1):34-38.LUO Z N,ZHAO G Q,LIU H.24 oat varieties of seed germination of salt resistance comprehensive evaluation.Grassland and Lawn,2012,32(1):34-38.
[18]王晓龙.五种禾本科牧草生物学特性、农艺性状及抗逆性研究.呼和浩特:内蒙古农业大学硕士学位论文,2014.WANG X L.Evaluation of the biologic traits,agronomic characters and stress tolerance for five forage grasses.Master Thesis.Hohhot:Inner Mongolia Agricultural University,2014.
[19]武俊英,刘景辉,翟利剑,李倩,李立军.不同品种燕麦种子萌发和幼苗生长的耐盐性.生态学杂志,2009,28(10):1960-1965.WU J Y,LIU J H,ZHAI L J,LI Q,LI L J.Salt-tolerance of seed germination and seedling growth of different oat varieties.Ecology Journal,2009,28(10):1960-1965.
[20]史燕山,骆建霞,黄家珍,叶军,魏巍.盐胁迫对7种草本地被植物种子萌发的影响.天津农学院学报,2007,14(4):1-4.SHI Y S,LUO J X,HUANG J Z,YE J,WEI E.Effects of salt stress on seed germination of seven herb ground cover plants.Journal of Tianjin Agricultural College,2007,14(4):1-4.
[21]LI M,WANG G X.Effect of drought Stress on activities of cell defense enzymes and lipid peroxidation in glycyrrhiza uralensis seedlings.Acta Ecoloigica Sinica,2002,22(4):503-507.
[22]陈莎莎,姚世响,袁军文,兰海燕.盐生植物灰绿藜对NaCl和NaHCO3胁迫的生理响应.新疆农业科学,2010,47(5):882-887.CHEN S S,YAO S X,YUAN J W,LAN H Y.Physiological responses of halophyte Chenopodium glaucum L.to NaCl and NaHCO3 stresses.Xinjiang Agricultural Sciences,2010,47(5):882-887.
[23]邓敏捷,张晓申,范国强,赵振利,董焱鹏,魏振.四倍体泡桐对盐胁迫生理响应的差异.中南林业科技大学学报,2013,33(11):42-46.DENG M J,ZHANG X S,FAN G Q,ZHAO Z L,DONG Y P,WEI Z.Comparative studies on physiological responses to salt stress in tetraploid paulownia plants.Journal of Central South University of Forestry and Technology,2013,33(11):42-46.
[24]洪森荣,尹明华.红芽芋驯化苗对盐胁迫的光合及生理响应.西北植物学报,2013,33(12):2499-2506.HONG S R,YIN M H.Photosynthetic and physiological responses of red bud taro transplantating seedlings under salt stress.Journal of Northwest Plants,2013,33(12):2499-2506.
[25]廖岩,陈桂珠.三种红树植物对盐胁迫的生理适应.生态学报,2007,27(6):2208-2214.LIAO Y,CHEN G Z.Research on physiological adaptability of three mangrove species to salt stress.Journal of Ecology,2007,27(6):2208-2214.
[26]张丽.平欧杂种榛抗盐碱生理机制研究及其耐盐性评价.北京:中国林业科学研究院,2015.ZHANG L.Study on the Physiological Mechanism of the Hybrid Hazel and Salinity Tolerance.Beijing:Chinese Academy of Forestry Sciences,2015.
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