西芹鲜根及根际区物浸提液处理后黄瓜枯萎病菌落直径及其分泌的3种细胞壁降解酶活性的变化
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摘要
为了探讨西芹鲜根及根际区物浸提液对黄瓜枯萎病菌的生理生化作用,在培养基条件下,将西芹鲜根及根际区物浸提液(蒸馏水、80%丙酮、80%乙醇)和黄瓜枯萎病菌进行共培养,144 h后测定黄瓜枯萎病菌菌落直径以及分泌的3种细胞壁降解酶活性。结果表明,共培养144h之后,鲜根及根际区物浸提液处理可以抑制菌落直径和CWDEs活性,鲜根丙酮浸提液处理的抑制作用最佳,菌落直径较丙酮对照下降了31.16%,CWDEs活性分别较对照下降了43.87%、13.05%和27.19%。说明西芹鲜根及根际区物浸提液中含有的物质能够抑制黄瓜枯萎病菌的生长,并降低其分泌的细胞壁降解酶活性,并且表现为鲜根浸提液效果优于根际区物浸提液,丙酮浸提液效果优于乙醇浸提液优于蒸馏水浸提液。
For investigate the effects of extracts in the parsley fresh root and root soil on physiological and biochemical aspects of Fusarium oxysporum f. sp. cucumebrium. Studied the changes of colony diameter and the activities of three cell wall degrading enzymes after 144 h of co-culture between the extracts( distilled water、80% acetone and 80% ethanol) of parsley fresh root and root soil with Fusarium oxysporium f. sp. cucumerinum in the medium. The results showed that after 144 h of co-culture,after treated with the extracts,colony diameter and CWDEs,activities were all less-than the contrasts,and the acetone extract of fresh root treatment was the best one,the colony diameter was lower 31. 16% than the acetone control,the CWDEs,activities were decreased by 43.87% 、13. 05% and 27. 19% than the acetone control. So the substances of the different extracts of parsley fresh root and root soil were inhibited the colony diameter and the activity of cell wall degrading enzyme. Fresh root extracts caused more distinct changes than root soil extracts,the most significant effects were noted by acetone extracts,followed by ethanol extracts and distilled water extracts.
For investigate the effects of extracts in the parsley fresh root and root soil on physiological and biochemical aspects of Fusarium oxysporum f. sp. cucumebrium. Studied the changes of colony diameter and the activities of three cell wall degrading enzymes after 144 h of co-culture between the extracts( distilled water、80% acetone and 80% ethanol) of parsley fresh root and root soil with Fusarium oxysporium f. sp. cucumerinum in the medium. The results showed that after 144 h of co-culture,after treated with the extracts,colony diameter and CWDEs,activities were all less-than the contrasts,and the acetone extract of fresh root treatment was the best one,the colony diameter was lower 31. 16% than the acetone control,the CWDEs,activities were decreased by 43.87% 、13. 05% and 27. 19% than the acetone control. So the substances of the different extracts of parsley fresh root and root soil were inhibited the colony diameter and the activity of cell wall degrading enzyme. Fresh root extracts caused more distinct changes than root soil extracts,the most significant effects were noted by acetone extracts,followed by ethanol extracts and distilled water extracts.
引文
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[6]魏彦梅,李蕾,陈磊,等.西芹鲜根及根际区物浸提液对黄瓜枯萎病菌化感作用机理的研究——浸提液处理后黄瓜叶片内防御酶活性的变化[J].内蒙古农业大学学报(自然科学版),2012,33(2):37-43.
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[10]徐宁,王超,魏珉,等.大葱根系分泌物对黄瓜种子萌芽和枯萎病病原菌的化感作用及其GC–MS分析[J].园艺学报,2012,39(8):1511-1520.
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[12]马志伟,陈慧,蒙美莲,等.玉米、小麦、大豆根系水溶性分泌物对马铃薯枯萎病菌的影响[J].中国马铃薯,2016,30(4):235-239.
[13]Sowbhagya HB,Srinivas P,Krishnamurthy N.Effect of enzymes on extraction of volatiles from celery seeds.[J].Food Chemistry,2010,120:230-234.
[14]Cardin L,Vincenot L,Balesdent M H.First report of Pilidium concavum on Bergenia crassifolia in France[J].Plant Disease,2009,93(5):548.
[15]许春景,吴玉星,戴青春,等.苹果树腐烂病多聚半乳糖醛酸基因Vmpg7和Vmpg8的功能[J].中国农业科学,2016,49(8):1489-1498.
[16]倪璇,姜雪,李雅乾,等.玉米弯孢叶斑病菌ATMT突变株构建及致病力分析[J].中国生物工程杂志,2016,36(1):23-28.
[17]杨志敏,毕阳,李永才,等.马铃薯干腐病菌侵染过程中切片组织细胞壁降解酶的变化[J].中国农业科学,2012,45(1):127-134.
[18]Zhao S,Du C M,Tian C Y.Suppression of Fusarium oxysporum and induced resistance of plants involved in the biocontrol of Cucumber Fusarium wilt by Streptomyces bikiniensis HD-087[J].World Journal of Microbiology and Biotrchnology,2012,28:2919-2927.
[19]Kikot GE,Hours RA,Alconada TM.Contribution of cell wall degrading enzymes to pathogenesis of?Fusarium graminearum:a review[J].Journal of Basic Microbiology,2009,49(3):231-240.
[20]高晓敏,马立国,郝静,等.西芹根物质四次酮层物对黄瓜枯萎病菌细胞壁降解酶活性及镰刀菌酸含量的影响[J].生态学杂志,2014,33(12):3388-3394.
[2]赵丽娅,李文庆,唐龙翔,等.有机肥对黄瓜枯萎病的防治效果及防病机理研究[J].土壤学报,2015,52(6):1383-1391.
[3]赵帅,杜春梅,田长彦.黄瓜枯萎病综合防治研究进展[J].中国农学通报,2014,30(7):254-259.
[4]张波,云兴福.西芹干根提取物对黄瓜枯萎病菌化感作用的研究[J].内蒙古农业大学学报(自然科学版),2010,31(1):136-140.
[5]曹阳.西芹腐根及腐根际区物浸提液处理对黄瓜枯萎病菌化感作用的研究[D].呼和浩特:内蒙古农业大学.2009.
[6]魏彦梅,李蕾,陈磊,等.西芹鲜根及根际区物浸提液对黄瓜枯萎病菌化感作用机理的研究——浸提液处理后黄瓜叶片内防御酶活性的变化[J].内蒙古农业大学学报(自然科学版),2012,33(2):37-43.
[7]陈磊,李蕾,项鹏宇,等.西芹挥发物对黄瓜枯萎病菌的化感作用[J].生态学杂志,2012,31(4):877-881.
[8]Rice EL.Allelopathy.2nd edition[M].New York:Academic Press,1984.
[9]庄敬华,杨长成,唐树戈,等.几种设施蔬菜根系浸提液对甜瓜的化感作用[J].种子,2009,28(11):94-96.
[10]徐宁,王超,魏珉,等.大葱根系分泌物对黄瓜种子萌芽和枯萎病病原菌的化感作用及其GC–MS分析[J].园艺学报,2012,39(8):1511-1520.
[11]周俐利.荞麦根系分泌液对甘蓝根肿病的抑制效果及其抑病机理研究[D].重庆市:西南大学,2017.
[12]马志伟,陈慧,蒙美莲,等.玉米、小麦、大豆根系水溶性分泌物对马铃薯枯萎病菌的影响[J].中国马铃薯,2016,30(4):235-239.
[13]Sowbhagya HB,Srinivas P,Krishnamurthy N.Effect of enzymes on extraction of volatiles from celery seeds.[J].Food Chemistry,2010,120:230-234.
[14]Cardin L,Vincenot L,Balesdent M H.First report of Pilidium concavum on Bergenia crassifolia in France[J].Plant Disease,2009,93(5):548.
[15]许春景,吴玉星,戴青春,等.苹果树腐烂病多聚半乳糖醛酸基因Vmpg7和Vmpg8的功能[J].中国农业科学,2016,49(8):1489-1498.
[16]倪璇,姜雪,李雅乾,等.玉米弯孢叶斑病菌ATMT突变株构建及致病力分析[J].中国生物工程杂志,2016,36(1):23-28.
[17]杨志敏,毕阳,李永才,等.马铃薯干腐病菌侵染过程中切片组织细胞壁降解酶的变化[J].中国农业科学,2012,45(1):127-134.
[18]Zhao S,Du C M,Tian C Y.Suppression of Fusarium oxysporum and induced resistance of plants involved in the biocontrol of Cucumber Fusarium wilt by Streptomyces bikiniensis HD-087[J].World Journal of Microbiology and Biotrchnology,2012,28:2919-2927.
[19]Kikot GE,Hours RA,Alconada TM.Contribution of cell wall degrading enzymes to pathogenesis of?Fusarium graminearum:a review[J].Journal of Basic Microbiology,2009,49(3):231-240.
[20]高晓敏,马立国,郝静,等.西芹根物质四次酮层物对黄瓜枯萎病菌细胞壁降解酶活性及镰刀菌酸含量的影响[J].生态学杂志,2014,33(12):3388-3394.