HPLC法同时测定桂灵片中6种生物碱的含量
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摘要
目的:建立HPLC法同时测定桂灵片中6种生物碱的含量。方法:采用Agilent Eclipse Plus-C18色谱柱(4. 6 mm×250mm,4. 6μm),以乙腈(A)-0. 1%磷酸(B)为流动相,梯度洗脱,流速1. 0 m L·min-1,测定波长:吗啡、盐酸麻黄碱、盐酸伪麻黄碱、盐酸甲基麻黄碱、磷酸可待因210 nm;盐酸罂粟碱251 nm。结果:吗啡、盐酸麻黄碱、盐酸伪麻黄碱、盐酸甲基麻黄碱、磷酸可待因和盐酸罂粟碱进样量分别在0. 001 3～0. 13μg(r=0. 999 9),0. 002 5～0. 25μg(r=0. 999 9),0. 002 5～0. 25μg(r=0. 999 9),0. 005 4～0. 27μg(r=0. 999 9),0. 002 6～0. 26μg(r=0. 999 9),0. 000 5～0. 11μg(r=0. 999 9)范围内呈良好线性关系,平均回收率(n=6)分别为101. 6%、100. 2%、95. 3%、99. 2%、103. 5%、100. 2%。结论:该方法可以用于桂灵片的质量控制。
Objective: To establish an HPLC method for simultaneous determination of six alkaloids in Guiling tablets,and to analyze the results by principal component analysis. Methods: Separation was performed on a Agilent Eclipse Plus-C18 column( 4. 6 mm × 250 mm,5 μm). And the mobile phase consisted of acetonitrile( A) and 0. 1% phosphoric acid( B) with gradient elution at a flow rate of1. 0 mL·min-1. The detection wavelength was 210 nm for morphine、ephedrine、pseudoephedrine、methylephedrine hydrochloride、codeine and 251 nm for papaverine. Results: Morphine、ephedrine、pseudoephedrine、methylephedrine hydrochloride、codeine and papaverine showed good linearity in the ranges of 0. 001 3 ～ 0. 13 μg( r = 0. 999 9),0. 002 5 ～ 0. 25 μg( r = 0. 999 9),0. 002 5 ～ 0. 25 μg( r =0. 999 9),0. 005 4 ～ 0. 27 μg( r = 0. 999 9),0. 002 6 ～ 0. 26 μg( r = 0. 999 9),0. 000 5 ～ 0. 11 μg( r = 0. 999 9). The average recoveries( n = 6) were 101. 6% 、100. 2% 、95. 3% 、99. 2% 、103. 5% 、100. 2%. Principal component analysis extracted two principal components,and explained 91. 7% of the group differences,which better reflected the difference between groups. Conclusion: The method is applicable for the quality control of Guiling tablets.
Objective: To establish an HPLC method for simultaneous determination of six alkaloids in Guiling tablets,and to analyze the results by principal component analysis. Methods: Separation was performed on a Agilent Eclipse Plus-C18 column( 4. 6 mm × 250 mm,5 μm). And the mobile phase consisted of acetonitrile( A) and 0. 1% phosphoric acid( B) with gradient elution at a flow rate of1. 0 mL·min-1. The detection wavelength was 210 nm for morphine、ephedrine、pseudoephedrine、methylephedrine hydrochloride、codeine and 251 nm for papaverine. Results: Morphine、ephedrine、pseudoephedrine、methylephedrine hydrochloride、codeine and papaverine showed good linearity in the ranges of 0. 001 3 ～ 0. 13 μg( r = 0. 999 9),0. 002 5 ～ 0. 25 μg( r = 0. 999 9),0. 002 5 ～ 0. 25 μg( r =0. 999 9),0. 005 4 ～ 0. 27 μg( r = 0. 999 9),0. 002 6 ～ 0. 26 μg( r = 0. 999 9),0. 000 5 ～ 0. 11 μg( r = 0. 999 9). The average recoveries( n = 6) were 101. 6% 、100. 2% 、95. 3% 、99. 2% 、103. 5% 、100. 2%. Principal component analysis extracted two principal components,and explained 91. 7% of the group differences,which better reflected the difference between groups. Conclusion: The method is applicable for the quality control of Guiling tablets.
引文
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[7]李翔,刘皈阳,马建丽,等.HPLC法测定麻杏口服液中盐酸麻黄碱和盐酸伪麻黄碱的含量[J].药物分析杂志,2014,34(1):190-192.
[8]刘文炜,高玉琼,刘建华,等.RP-HPLC法同时测定哮喘片中吗啡、盐酸麻黄碱、磷酸可待因的含量[J].药物分析杂志,2009,29(5):731-734.
[9]黎春彤,李翔,马建丽,等.HPLC同时测定防风通圣丸中盐酸麻黄碱和盐酸伪麻黄碱的含量[J].药物分析杂志,2016,36(1):176-180.
[10]刘永利,袁浩,李冬梅,等.UPLC法测定罂粟壳中3种生物碱类成分含量[J].中药材,2011,34(9):1386-1388.
[11]刘红,魏柳珍.食品中罂粟壳成分残留检测技术及前处理技术研究进展[J].药物分析杂志,2017,37(10):1747-1753.
[12]李纯,栗建明,顾利红.RPLC-MS/MS同时测定罂粟壳药材中5种生物碱的含量[J].今日药学,2018,28(3):152-161.
[13]刘新豫,司晓萍,唐辉,等.RP-HPLC法测定热感赛比斯坦颗粒中3种生物碱成分的含量[J].药物分析杂志,2017,37(5):897-902.
[14]杨德斌,仝燕,马振山,等.HPLC法同时测定小儿平喘祛痰颗粒中盐酸麻黄碱、盐酸伪麻黄碱、苦杏仁苷含量[J].中国中药杂志,2013,38(5):687-690.
[15]李冬梅,庄爱爱,苏建,等.麻杏止咳糖浆含量测定方法研究[J].中国药业,2017,26(12):27-29.
[2]丁丽丽,施松善,崔健,等.麻黄化学成分与药理作用研究进展[J].中国中药杂志,2006,31(20):1661-1664.
[3]何遂庆,施心建.含罂粟壳中成药质量标准分析[J].浙江中医药大学学报,2006,30(4):422-423.
[4]虞金宝,吕武清,李晶,等.桂灵胶囊中麻黄碱含量测定研究[J].江西中医学报,2004,16(2):60-61.
[5]周春菊,唐勇.高效液相色谱法测定桂灵胶囊中盐酸麻黄碱的含量[J].中南药学,2008,6(2):205-207.
[6]倪亚娜,赵俊华,赵俊秋.桂灵丸鉴别方法的研究[J].中国医药指南,2012,10(33):80-81.
[7]李翔,刘皈阳,马建丽,等.HPLC法测定麻杏口服液中盐酸麻黄碱和盐酸伪麻黄碱的含量[J].药物分析杂志,2014,34(1):190-192.
[8]刘文炜,高玉琼,刘建华,等.RP-HPLC法同时测定哮喘片中吗啡、盐酸麻黄碱、磷酸可待因的含量[J].药物分析杂志,2009,29(5):731-734.
[9]黎春彤,李翔,马建丽,等.HPLC同时测定防风通圣丸中盐酸麻黄碱和盐酸伪麻黄碱的含量[J].药物分析杂志,2016,36(1):176-180.
[10]刘永利,袁浩,李冬梅,等.UPLC法测定罂粟壳中3种生物碱类成分含量[J].中药材,2011,34(9):1386-1388.
[11]刘红,魏柳珍.食品中罂粟壳成分残留检测技术及前处理技术研究进展[J].药物分析杂志,2017,37(10):1747-1753.
[12]李纯,栗建明,顾利红.RPLC-MS/MS同时测定罂粟壳药材中5种生物碱的含量[J].今日药学,2018,28(3):152-161.
[13]刘新豫,司晓萍,唐辉,等.RP-HPLC法测定热感赛比斯坦颗粒中3种生物碱成分的含量[J].药物分析杂志,2017,37(5):897-902.
[14]杨德斌,仝燕,马振山,等.HPLC法同时测定小儿平喘祛痰颗粒中盐酸麻黄碱、盐酸伪麻黄碱、苦杏仁苷含量[J].中国中药杂志,2013,38(5):687-690.
[15]李冬梅,庄爱爱,苏建,等.麻杏止咳糖浆含量测定方法研究[J].中国药业,2017,26(12):27-29.