不同标本类型C反应蛋白检测结果的一致性分析
|
摘要
目的探讨PENTRA MS CRP分析仪检测全血标本与TBA2000FR全自动生化分析仪检测血浆、血清标本C反应蛋白(CRP)结果的可比性及相关性。方法随机留取2017年5-7月东部战区总医院(原八二医院)93例门诊及住院患者EDTA-K2抗凝血与非抗凝血标本(57例抗凝标本,36例血清标本),分别采用PENTRA MS CRP分析仪检测全血标本CRP浓度,TBA2000FR全自动生化分析仪检测血浆与血清CRP浓度。根据全血CRP浓度,将受试者分为低值组(<5μg/mL)、中值组(5～20μg/mL)和高值组(>20μg/mL),分别对全血CRP浓度与血浆、血清CRP浓度进行比对分析。结果在低值组、中值组及高值组中,全血CRP与血浆CRP之间(r值分别为0.941、0.974、0.964),全血CRP与血清CRP之间(r值分别为0.995、0.966、0.996)均呈良好的相关性,差异均有统计学意义(P<0.05),其中全血CRP与血清CRP之间差异在临床可接受范围内。结论血浆标本不适于直接在TBA2000FR全自动生化分析仪检测,全血标本与血清标本分别在2个检测系统的检验结果具有一致性。
Objective To investigate the comparability and correlation of whole blood,plasma and serum C-reactive protein(CRP)in PENTRA MS CRP analyzer and TBA2000 FR automatic biochemical analyzer.Methods 93 EDTA-K2 anticoagulant and non-anticoagulant specimens from outpatients and inpatients in our hospital were randomly collected(57 anticoagulant specimens,36 serum specimens).The CRP concentration of whole blood specimen was detected by PENTRA MS CRP analyzer,The CRP concentration of plasma and serum specimens was detected by TBA2000 FR automatic biochemical analyzer. The subjects divided into the low value(<5 μg/mL),median value(5-20 μg/mL)and high value(>20 μg/mL)group according to the CRP concentration of whole blood. The whole blood,plasma and serum CRP concentration was compared and analyzed.Results There was a good correlation between plasma CRP and whole blood CRP(R values were 0.941,0.974,0.964 respectively)and between serum CRP and whole blood CRP(R values were 0.995,0.966,0.996 respectively)in low value group,middle value group and high value group,and there were significant differences(P<0.05). The bias of the whole blood CRP and serum CRP is within the clinical acceptable range.Conclusion Plasma samples are not suitable for direct detection by TBA2000 FR automatic biochemical analyzer. The results of whole blood samples and serum samples are consistent in the two detecting systems.
Objective To investigate the comparability and correlation of whole blood,plasma and serum C-reactive protein(CRP)in PENTRA MS CRP analyzer and TBA2000 FR automatic biochemical analyzer.Methods 93 EDTA-K2 anticoagulant and non-anticoagulant specimens from outpatients and inpatients in our hospital were randomly collected(57 anticoagulant specimens,36 serum specimens).The CRP concentration of whole blood specimen was detected by PENTRA MS CRP analyzer,The CRP concentration of plasma and serum specimens was detected by TBA2000 FR automatic biochemical analyzer. The subjects divided into the low value(<5 μg/mL),median value(5-20 μg/mL)and high value(>20 μg/mL)group according to the CRP concentration of whole blood. The whole blood,plasma and serum CRP concentration was compared and analyzed.Results There was a good correlation between plasma CRP and whole blood CRP(R values were 0.941,0.974,0.964 respectively)and between serum CRP and whole blood CRP(R values were 0.995,0.966,0.996 respectively)in low value group,middle value group and high value group,and there were significant differences(P<0.05). The bias of the whole blood CRP and serum CRP is within the clinical acceptable range.Conclusion Plasma samples are not suitable for direct detection by TBA2000 FR automatic biochemical analyzer. The results of whole blood samples and serum samples are consistent in the two detecting systems.
引文
[1]叶应妩,王毓三,申子瑜.全国临床检验操作规程[M].3版.南京:东南大学出版社,2006:590-592.
[2]诸兴明,陈阳,杜宇平,等.急性脑梗死患者血清胱抑素C和C反应蛋白水平变化的研究[J].东南国防医药,2014,16(3):275-277.
[3]陈丽萍,陈岩,张强,等.瑞舒伐他汀序贯治疗对急性冠脉综合征患者血清缺血修饰清蛋白、C反应蛋白、髓过氧化物酶的影响[J].东南国防医药,2015,17(3):272-274,277.
[4]廖春明.CRP检测在血液相关疾病中的临床意义[J].临床合理用药杂志,2017,10(4):34-35.
[5]张晓浩,段作伟,刘德志,等.血清C反应蛋白和基底节区脑出血早期神经功能恶化的相关性分析[J].医学研究生学报,2014,27(12):1277-1280.
[6]周爱国,苏勇,陆奎英.迈瑞BC-5390全自动血液分析仪检测C反应蛋白的性能研究[J].医疗卫生装备,2016,37(12):104-107.
[7]方伟,何艳群,焦炳欣.2种检测系统C反应蛋白结果测定的比较[J].国际检验医学杂志,2016,937(18):2618-2619.
[8]郭平,王剑飚,陈骊婷,等.关于血细胞分析仪CRP一体机临床应用性能的研究[J].检验医学与临床,2016(9):1216-1219.
[9]王立京.免疫透射比浊法与干式免疫荧光法检测C反应蛋白的结果差异[J].国际检验医学杂志,2015,36(16):2406-2407.
[10]劳丽芬.小儿感染性疾病中C-反应蛋白的诊断价值分析[J].中国医学创新,2013,10(3):61-62.
[11]曾凤群,丘仲柳,利雯秀,等.全血与血浆标本CRP检测的对比分析[J].中外医学研究,2016,14(20):4-6.
[12]张晓阳,杨志兵,杨春显.两种方法检测C反应蛋白结果的可比性分析[J].国际检验医学杂志,2014,35(9):1214-1215.
[13]华关民,唐荣德,梁剑宁,等.血清CRP与hs-CRP检测值比较与相关性分析[J].中国医学创新,2014,11(3):32-34.
[14]汪怀周,陈燕,岳展伊,等.C反应蛋白测定两种检测系统间结果的比较[J].现代检验医学杂志,2016,31(3):127-130.
[15]裴兵.全血C反应蛋白检测影响因素的校正方法及临床应用[J].国际检验医学杂志,2013,34(2):192-194.
[16]肖光军,刘艳婷,范婵,等.红细胞压积对全血C-反应蛋白检测结果的影响[J].现代临床医学,2017,43(6):438-441.
[17]杜婷,藏小晴.i-CHROMA分析仪测定CRP及hs-CRP两种抗凝剂应用比较[J].中国保健营养,2013,23(5):2737.
[18]易鹏.两种方法检测CRP和HSCRP的结果对比[J].华夏医学,2015,28(4):95-98.
[2]诸兴明,陈阳,杜宇平,等.急性脑梗死患者血清胱抑素C和C反应蛋白水平变化的研究[J].东南国防医药,2014,16(3):275-277.
[3]陈丽萍,陈岩,张强,等.瑞舒伐他汀序贯治疗对急性冠脉综合征患者血清缺血修饰清蛋白、C反应蛋白、髓过氧化物酶的影响[J].东南国防医药,2015,17(3):272-274,277.
[4]廖春明.CRP检测在血液相关疾病中的临床意义[J].临床合理用药杂志,2017,10(4):34-35.
[5]张晓浩,段作伟,刘德志,等.血清C反应蛋白和基底节区脑出血早期神经功能恶化的相关性分析[J].医学研究生学报,2014,27(12):1277-1280.
[6]周爱国,苏勇,陆奎英.迈瑞BC-5390全自动血液分析仪检测C反应蛋白的性能研究[J].医疗卫生装备,2016,37(12):104-107.
[7]方伟,何艳群,焦炳欣.2种检测系统C反应蛋白结果测定的比较[J].国际检验医学杂志,2016,937(18):2618-2619.
[8]郭平,王剑飚,陈骊婷,等.关于血细胞分析仪CRP一体机临床应用性能的研究[J].检验医学与临床,2016(9):1216-1219.
[9]王立京.免疫透射比浊法与干式免疫荧光法检测C反应蛋白的结果差异[J].国际检验医学杂志,2015,36(16):2406-2407.
[10]劳丽芬.小儿感染性疾病中C-反应蛋白的诊断价值分析[J].中国医学创新,2013,10(3):61-62.
[11]曾凤群,丘仲柳,利雯秀,等.全血与血浆标本CRP检测的对比分析[J].中外医学研究,2016,14(20):4-6.
[12]张晓阳,杨志兵,杨春显.两种方法检测C反应蛋白结果的可比性分析[J].国际检验医学杂志,2014,35(9):1214-1215.
[13]华关民,唐荣德,梁剑宁,等.血清CRP与hs-CRP检测值比较与相关性分析[J].中国医学创新,2014,11(3):32-34.
[14]汪怀周,陈燕,岳展伊,等.C反应蛋白测定两种检测系统间结果的比较[J].现代检验医学杂志,2016,31(3):127-130.
[15]裴兵.全血C反应蛋白检测影响因素的校正方法及临床应用[J].国际检验医学杂志,2013,34(2):192-194.
[16]肖光军,刘艳婷,范婵,等.红细胞压积对全血C-反应蛋白检测结果的影响[J].现代临床医学,2017,43(6):438-441.
[17]杜婷,藏小晴.i-CHROMA分析仪测定CRP及hs-CRP两种抗凝剂应用比较[J].中国保健营养,2013,23(5):2737.
[18]易鹏.两种方法检测CRP和HSCRP的结果对比[J].华夏医学,2015,28(4):95-98.