非洲紫罗兰叶片外植体诱导分化研究
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摘要
为了探索非洲紫罗兰叶片外植体适宜的诱导体系,对叶片离体条件下快速繁殖技术进行了研究。结果表明:改良MS培养基诱导率高于未改良的MS培养基,最优诱导培养基组合为MS改良+6-BA2.0mg·L-1+IBA0.2mg·L-1+GA31.0mg·L-1,诱导频率可达100%,芽增殖近10倍,17d即可出芽,大大缩短了育苗周期,提高了扩繁效率。
In order to explore suitable induction system of Saintpaulia ionanthaleaf explant,the rapid propagation technology of leaf in vitro was studied.The results showed that the induction rate of modified MS medium was higher than common MS medium,the optimal medium was improved by MS+6-BA2.0 mg·L-1+IBA0.2mg·L-1+GA31.0mg·L-1,induction frequency could reach to 100%,proliferation of bud was nearly 10 times than common medium,produced buds after inducing 17 days,greatly shortened the cycle of seedling,and improved the efficiency of propagation.
In order to explore suitable induction system of Saintpaulia ionanthaleaf explant,the rapid propagation technology of leaf in vitro was studied.The results showed that the induction rate of modified MS medium was higher than common MS medium,the optimal medium was improved by MS+6-BA2.0 mg·L-1+IBA0.2mg·L-1+GA31.0mg·L-1,induction frequency could reach to 100%,proliferation of bud was nearly 10 times than common medium,produced buds after inducing 17 days,greatly shortened the cycle of seedling,and improved the efficiency of propagation.
引文
[1]谢雄辉,谢怡青,吴炯光.非洲紫罗兰的组织培养和快速繁殖[J].农业科技通讯,2006(8):53.
[2]龚宇舟,夏明,雷洁琼,等.我国非洲紫罗兰组织培养研究进展[J].现代农业科技,2013(2):169,180.
[3]纪昭辉.非洲紫罗兰的组织培养和快速繁殖初报[J].吉林林业科技,2003(8):5,8.
[4]张晓军.非洲紫罗兰组织离体培养及快速繁殖[J].东北林业大学学报,2004,32(2):107-108.
[5]刘爱华.非洲紫罗兰的组织培养[J].农业与技术,2005,25(5):119-122.
[6]谢雄辉.非洲紫罗兰的组织培养及快速繁殖[J].农业科技通讯,2006(8):53.
[7]龚宇舟,刘清波,雷洁琼,等.非洲紫罗兰愈伤组织诱导及植株再生[J].农业工程,2013,3(3):120-122.
[2]龚宇舟,夏明,雷洁琼,等.我国非洲紫罗兰组织培养研究进展[J].现代农业科技,2013(2):169,180.
[3]纪昭辉.非洲紫罗兰的组织培养和快速繁殖初报[J].吉林林业科技,2003(8):5,8.
[4]张晓军.非洲紫罗兰组织离体培养及快速繁殖[J].东北林业大学学报,2004,32(2):107-108.
[5]刘爱华.非洲紫罗兰的组织培养[J].农业与技术,2005,25(5):119-122.
[6]谢雄辉.非洲紫罗兰的组织培养及快速繁殖[J].农业科技通讯,2006(8):53.
[7]龚宇舟,刘清波,雷洁琼,等.非洲紫罗兰愈伤组织诱导及植株再生[J].农业工程,2013,3(3):120-122.