威宁绵羊SOCS7和GFAP基因组织表达研究
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摘要
试验旨在检测细胞因子信号传导抑制蛋白7(suppressor of cytokine signaling 7,SOCS7)和胶质纤维酸性蛋白(glial fibrillary acidic protein,GFAP)基因在威宁绵羊不同组织中的表达水平。以6月龄、1周岁和2周岁的威宁绵羊公、母羊为研究对象,采用实时荧光定量PCR测定威宁绵羊不同性别及不同生长阶段心脏、肝脏、脾脏、肺脏、肾脏及脑6个组织中SOCS7和GFAP基因的相对表达量,从mRNA水平上的探究两个基因之间的表达规律。结果显示,SOCS7基因在威宁绵羊不同组织中均有不同程度的表达,其中脾脏中相对表达量最高,其次是肺脏、肾脏、心脏、肝脏和脑组织,随着威宁绵羊年龄的增大,SOCS7基因在组织中的相对表达量呈现小幅度上升的趋势;GFAP基因在威宁绵羊脑组织中相对表达量最高,其余组织中表达量较低,随着年龄增长总体呈现下降趋势。从性别差异来看,威宁绵羊SOCS7基因相对表达量母羊普遍高于公羊,而GFAP基因则是公羊普遍高于母羊,推测SOCS7基因很有可能负向调控GFAP基因的表达。
This study was aimed to detect the expression of suppressor of cytokine signaling 7(SOCS7) and glial fibrillary acidic protein(GFAP) genes in different tissues of Weining sheep.This experiment took male and female Weining sheep aged 6 months,1 year and 2 years as research objects,the relative expression of SOCS7 and GFAP genes in six tissues of heart,liver,spleen,lung,kidney and brain at different sex and growth stages were determined by Real-time quantitative PCR,and the mRNA level expression pattern of SOCS7 and GFAP genes were explored.The results showed that SOCS7 gene was expressed in different tissues of Weining sheep,the expression of spleen was the highest,followed by lung,kidney,heart,liver and brain,and the relative expression of SOCS7 gene in Weining sheep slightly increased with age.The relative expression of GFAP gene was the highest in brain of Weining sheep,while the rest of the tissues showed a low expression level,which showed a downward trend with age.According to the genders difference,the relative expression of SOCS7 gene in female was generally higher than male,while that of GFAP gene in male was generally higher than female,it suggested that SOCS7 gene might negatively regulate the expression of GFAP gene.
This study was aimed to detect the expression of suppressor of cytokine signaling 7(SOCS7) and glial fibrillary acidic protein(GFAP) genes in different tissues of Weining sheep.This experiment took male and female Weining sheep aged 6 months,1 year and 2 years as research objects,the relative expression of SOCS7 and GFAP genes in six tissues of heart,liver,spleen,lung,kidney and brain at different sex and growth stages were determined by Real-time quantitative PCR,and the mRNA level expression pattern of SOCS7 and GFAP genes were explored.The results showed that SOCS7 gene was expressed in different tissues of Weining sheep,the expression of spleen was the highest,followed by lung,kidney,heart,liver and brain,and the relative expression of SOCS7 gene in Weining sheep slightly increased with age.The relative expression of GFAP gene was the highest in brain of Weining sheep,while the rest of the tissues showed a low expression level,which showed a downward trend with age.According to the genders difference,the relative expression of SOCS7 gene in female was generally higher than male,while that of GFAP gene in male was generally higher than female,it suggested that SOCS7 gene might negatively regulate the expression of GFAP gene.
引文
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[10] 阮涌,许厚强,易鸣,等.贵州威宁绵羊生产及屠宰性能测定与分析[J].黑龙江畜牧兽医,2017,19:39-45.RUAN Y,XU H Q,YI M,et al.Measurement and analysis on production and slaughtering characteristics of Guizhou Weining sheep[J].Heilongjiang Animal Science and Veterinary Medicine,2017,19:39-45.(in Chinese)
[11] 王振,申小云,盘道兴,等.威宁绵羊和贵州半细毛羊STAT5b基因部分外显子SNP研究[J].基因组学与应用生物学,2015,34(5):938-944.WANG Z,SHEN X Y,PAN D X,et al.Study on SNP of STAT5b part of the exon in Weining sheep and Guizhou Semi-fine Wool sheep[J].Genomics and Applied Biology,2015,34(5):938-944.(in Chinese)
[12] 易鸣,刘章忠,程朝友,等.威宁绵羊体尺体重和肉用性能测定及其相关分析[J].黑龙江畜牧兽医,2018,2:63-64.YI M,LIU Z Z,CHENG C Y,et al.Measurement and correlation analysis of body weight and meat performance of Weining sheep[J].Heilongjiang Animal Science and Veterinary Medicine,2018,2:63-64.(in Chinese)
[13] KREBA D L,METEALF D,MERSON T D,et al.Development of hydrocephalus in mice lacking SOCS7[J].Proceedings of the National Academy of Sciences of the United States of America,2004,101(43):15446-15451.
[14] WERY M.The suppressor of cytokine signaling (SOCS)-7 interacts with the actin cytoskeleton through vinexin[J].Experimental Cell Research,2004,298(1):239-248.
[15] 张耀荣,孟风艳,李芳莲,等.猪SOCS7基因克隆、组织表达图谱分析及剪接变体鉴定[J].四川大学学报(自然科学版),2015,52(1):210-216.ZHANG Y R,MENG F Y,LI F L,et al.Characterization of SOCS7 (suppressor of cytokine signaling 7) gene in pigs:Cloning,tissue expression,and identification of its alternative splicing variants[J].Journal of Sichuan University (Natural Science Edition),2015,52(1):210-216.(in Chinese)
[16] 张冬玲,肖谦,罗会琼,等.血管紧张素(1-7)对糖尿病大鼠海马GFAP、GDNF表达和认知功能的影响[J].南方医科大学学报,2015,35(5):646-651.ZHANG D L,XIAO Q,LUO H Q,et al.Effects of angiotensin(1-7) on hippocampal expressions of GFAP and GDNF and cognitive function in rats with diabetes mellitus[J].Journal of Southern Medical University,2015,35(5):646-651.(in Chinese)
[17] 牛莉莉,温科奇,熊鹏,等.首发精神分裂症治疗前后血清NGF、BDNF、GFAP与临床症状的相关性研究[J].精神医学杂志,2015,28(1):10-12.NIU L L,WEN K Q,XIONG P,et al.Correlation between pre-and post-treatment serum levels of NGF,BDNF,GFAP and severity of clinical symptoms inpatients with first-episode schizophrenia[J].Journal of Psychiatry,2015,28(1):10-12.(in Chinese)
[18] 汝丽娟,裴海涛,郭云良.BMP-7对大鼠脑缺血/再灌注损伤后nestin和GFAP表达的影响[J].中国药理学通报,2011,27(12):1718-1723.RU L J,PEI H T,GUO Y L.Effect of bone morphogenetic protein-7 on expressions of nestin and GFAP in rats with cerebral ischemia reperfusion injury[J].Chinese Pharmacological Bulletin,2011,27(12):1718-1723.(in Chinese)
[19] 黎天尊,晏怡,刘强,等.细胞凋亡信号调节蛋白1对大鼠脊髓损伤后GFAP、vimentin表达及后肢运动功能的影响[J].南方医科大学学报,2015,35(6):795-800.LI T Z,YAN Y,LIU Q,et al.Effect of suppressing apoptosis signal regulating kinase 1 on GFAP and vimentin expression and hindlimb mobility in rats after spinal cord injury[J].Journal of Southern Medical University,2015,35(6):795-800.(in Chinese)
[20] 陈曦,洪响生,吴慧敏,等.稀有鮈鲫神经发育相关基因的克隆、同源性分析及组织分布[J].生态毒理学报,2016,11(1):173-181.CHEN X,HONG X S,WU H M,et al.Cloning,homology analysis,and distribution of neural development related genes of Chinese rare minnow (Gobiocypris rarus)[J].Asian Journal of Ecotoxicology,2016,11(1):173-181.(in Chinese)
[21] TAGA T,FUKUA S.Role of IL-6 in the neural stem cell differentiation[J].Clinical Reviews in Allergy & Immunology,2005,28(3):249-256.
[22] 崔远武,周震,张琳琳,等.从JAK/STAT信号转导通路探讨益肾化浊方对神经干细胞定向分化的影响[J].中医杂志,2016,57(6):512-515.CUI Y W,ZHOU Z,ZHANG L L,et al.Effects of Yishen Huazhuo Fang on directional differentiation of neural stem cells based on JAK/STAT signal transduction pathway [J].Journal of Traditional Chinese Medicine,2016,57(6):512-515.(in Chinese)
[23] 刘敬贤,夏永智,王富贵,等.IL-1β通过JAK2-STAT3促进大鼠脊髓损伤后胶质瘢痕形成[J].基础医学与临床,2017,37(5):668-675.LIU J X,XIA Y Z,WANG F G,et al.IL-1β promotes glial scar formation after spinal cord injury in rats by JAK2-STAT3[J].Basic & Clinical Medicine,2017,37(5):668-675.(in Chinese)
[2] LARSEN L,R?PKE C.Suppressors of cytokine signalling:SOCS[J].APMIS,2002,110(12):833-844.
[3] KILE B,ALEXANDER W.The suppressors of cytokine signalling (SOCS)[J].Cellular and Molecular Life Sciences,2001,58(11):1627-1635.
[4] KREBS D L,UREN R T,METEALF D,et al.SOCS-6 binds to insulin receptor substrate 4,and mice lacking the SOCS-6 gene exhibit mild growth retardation[J].Molecu-lar and Cellular Biology,2002,22(13):4567-4578.
[5] 王金洲,蒋自立,张强,等.务川黑牛SOCS基因家族表达调控分析[J].基因组学与应用生物学,2017,36(8):3172-3177.WANG J Z,JIANG Z L,ZHANG Q,et al.Expression and regulation analysis of SOCS gene family in Wuchuan Black cattle[J].Genomics and Applied Biology,2017,36(8):3172-3177.(in Chinese)
[6] SMITH D H,CHEN X H,XU B N,et al.Characterization of diffuse axonal pathology and selective hippocampal damage following inertial brain trauma in the pig[J].Journal of Neuropathology and Experimental Neurology,1997,56(7):822-834.
[7] RAJAN P,MEKAY R D.Multiple routes to astrocytic differentiation in the CNS[J].Journal of Neuroscience the Official Journal of the Society for Neuroscience,1998,18(10):3620-3629.
[8] KAHN M A,HUANG C J,CARUSO A,et al.Ciliary neurotrophic factor activates JAK/STAT signal transduction cascade and induces transcriptional expression of glial fibrillary acidic protein in glial cells[J].Journal of Neurochemistry,2010,68(4):1413-1423.
[9] 廖正录.贵州省家畜家禽品种资源动态信息调查报告(Ⅱ)[J].贵州畜牧兽医,1997,3:15-19.LIAO Z L.Investigation report on dynamic information of livestock and poultry variety resources in Guizhou province(Ⅱ)[J].Guizhou Journal of Animal Husbandry & Veterinary Medicine,1997,3:15-19.(in Chinese)
[10] 阮涌,许厚强,易鸣,等.贵州威宁绵羊生产及屠宰性能测定与分析[J].黑龙江畜牧兽医,2017,19:39-45.RUAN Y,XU H Q,YI M,et al.Measurement and analysis on production and slaughtering characteristics of Guizhou Weining sheep[J].Heilongjiang Animal Science and Veterinary Medicine,2017,19:39-45.(in Chinese)
[11] 王振,申小云,盘道兴,等.威宁绵羊和贵州半细毛羊STAT5b基因部分外显子SNP研究[J].基因组学与应用生物学,2015,34(5):938-944.WANG Z,SHEN X Y,PAN D X,et al.Study on SNP of STAT5b part of the exon in Weining sheep and Guizhou Semi-fine Wool sheep[J].Genomics and Applied Biology,2015,34(5):938-944.(in Chinese)
[12] 易鸣,刘章忠,程朝友,等.威宁绵羊体尺体重和肉用性能测定及其相关分析[J].黑龙江畜牧兽医,2018,2:63-64.YI M,LIU Z Z,CHENG C Y,et al.Measurement and correlation analysis of body weight and meat performance of Weining sheep[J].Heilongjiang Animal Science and Veterinary Medicine,2018,2:63-64.(in Chinese)
[13] KREBA D L,METEALF D,MERSON T D,et al.Development of hydrocephalus in mice lacking SOCS7[J].Proceedings of the National Academy of Sciences of the United States of America,2004,101(43):15446-15451.
[14] WERY M.The suppressor of cytokine signaling (SOCS)-7 interacts with the actin cytoskeleton through vinexin[J].Experimental Cell Research,2004,298(1):239-248.
[15] 张耀荣,孟风艳,李芳莲,等.猪SOCS7基因克隆、组织表达图谱分析及剪接变体鉴定[J].四川大学学报(自然科学版),2015,52(1):210-216.ZHANG Y R,MENG F Y,LI F L,et al.Characterization of SOCS7 (suppressor of cytokine signaling 7) gene in pigs:Cloning,tissue expression,and identification of its alternative splicing variants[J].Journal of Sichuan University (Natural Science Edition),2015,52(1):210-216.(in Chinese)
[16] 张冬玲,肖谦,罗会琼,等.血管紧张素(1-7)对糖尿病大鼠海马GFAP、GDNF表达和认知功能的影响[J].南方医科大学学报,2015,35(5):646-651.ZHANG D L,XIAO Q,LUO H Q,et al.Effects of angiotensin(1-7) on hippocampal expressions of GFAP and GDNF and cognitive function in rats with diabetes mellitus[J].Journal of Southern Medical University,2015,35(5):646-651.(in Chinese)
[17] 牛莉莉,温科奇,熊鹏,等.首发精神分裂症治疗前后血清NGF、BDNF、GFAP与临床症状的相关性研究[J].精神医学杂志,2015,28(1):10-12.NIU L L,WEN K Q,XIONG P,et al.Correlation between pre-and post-treatment serum levels of NGF,BDNF,GFAP and severity of clinical symptoms inpatients with first-episode schizophrenia[J].Journal of Psychiatry,2015,28(1):10-12.(in Chinese)
[18] 汝丽娟,裴海涛,郭云良.BMP-7对大鼠脑缺血/再灌注损伤后nestin和GFAP表达的影响[J].中国药理学通报,2011,27(12):1718-1723.RU L J,PEI H T,GUO Y L.Effect of bone morphogenetic protein-7 on expressions of nestin and GFAP in rats with cerebral ischemia reperfusion injury[J].Chinese Pharmacological Bulletin,2011,27(12):1718-1723.(in Chinese)
[19] 黎天尊,晏怡,刘强,等.细胞凋亡信号调节蛋白1对大鼠脊髓损伤后GFAP、vimentin表达及后肢运动功能的影响[J].南方医科大学学报,2015,35(6):795-800.LI T Z,YAN Y,LIU Q,et al.Effect of suppressing apoptosis signal regulating kinase 1 on GFAP and vimentin expression and hindlimb mobility in rats after spinal cord injury[J].Journal of Southern Medical University,2015,35(6):795-800.(in Chinese)
[20] 陈曦,洪响生,吴慧敏,等.稀有鮈鲫神经发育相关基因的克隆、同源性分析及组织分布[J].生态毒理学报,2016,11(1):173-181.CHEN X,HONG X S,WU H M,et al.Cloning,homology analysis,and distribution of neural development related genes of Chinese rare minnow (Gobiocypris rarus)[J].Asian Journal of Ecotoxicology,2016,11(1):173-181.(in Chinese)
[21] TAGA T,FUKUA S.Role of IL-6 in the neural stem cell differentiation[J].Clinical Reviews in Allergy & Immunology,2005,28(3):249-256.
[22] 崔远武,周震,张琳琳,等.从JAK/STAT信号转导通路探讨益肾化浊方对神经干细胞定向分化的影响[J].中医杂志,2016,57(6):512-515.CUI Y W,ZHOU Z,ZHANG L L,et al.Effects of Yishen Huazhuo Fang on directional differentiation of neural stem cells based on JAK/STAT signal transduction pathway [J].Journal of Traditional Chinese Medicine,2016,57(6):512-515.(in Chinese)
[23] 刘敬贤,夏永智,王富贵,等.IL-1β通过JAK2-STAT3促进大鼠脊髓损伤后胶质瘢痕形成[J].基础医学与临床,2017,37(5):668-675.LIU J X,XIA Y Z,WANG F G,et al.IL-1β promotes glial scar formation after spinal cord injury in rats by JAK2-STAT3[J].Basic & Clinical Medicine,2017,37(5):668-675.(in Chinese)
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