降解乙酰甲胺磷农药的芽孢杆菌分离鉴定与降解性能分析
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摘要
[目的]探究微生物对农药乙酰甲胺磷的生物降解作用。[方法]利用筛选培养基从土壤中分离乙酰甲胺磷降解性菌株,对其中一株降解性能较好的菌株YJ1进行分析,扩增菌株16S r DNA,测序并构建系统演化树,结合生理生化性质,初步确定其分类地位,并检测该菌株对乙酰甲胺磷的降解性能。[结果]乙酰甲胺磷降解菌株YJ1属于芽孢杆菌属,可以在乙酰甲胺磷为唯一碳源的环境中存活;该菌株在乙酰甲胺磷含量为1 200 mg/L的培养基中生长良好;在p H7~10碱性条件下,该菌株在乙酰甲胺磷液体培养基中生长较好,酸性条件不利于其生长;该菌株在72 h对乙酰甲胺磷降解率可达100%,具有很高的乙酰甲胺磷降解活性。[结论]所发现的芽孢杆菌YJ1可用于乙酰甲胺磷的生物降解。
[Objective]Analysis of biodegrading of acephate. [Methods]Acephate-biodegrading strains were isolated and a strain YJ1 with the best biodegradation property was further analyzed. Classification of the strain was based on molecular identification with 16 S r DNA,construction of the phylogenetic tree. The degradation of acephate of the strain was also studied. [Results]The strain YJ1 was identified as Bacillus. The strain can take acephate as the sole carbon source and it grew well in the medium with 1 200 mg/L acephate. The strain grew better in alkaline conditions of p H 7 to 10 than in acidic condition. The acephate degradation rates of the strain were both about 100% in 72 hours. [Conclusion]The strain YJ1 can be used for microbial degradation of acephate.
[Objective]Analysis of biodegrading of acephate. [Methods]Acephate-biodegrading strains were isolated and a strain YJ1 with the best biodegradation property was further analyzed. Classification of the strain was based on molecular identification with 16 S r DNA,construction of the phylogenetic tree. The degradation of acephate of the strain was also studied. [Results]The strain YJ1 was identified as Bacillus. The strain can take acephate as the sole carbon source and it grew well in the medium with 1 200 mg/L acephate. The strain grew better in alkaline conditions of p H 7 to 10 than in acidic condition. The acephate degradation rates of the strain were both about 100% in 72 hours. [Conclusion]The strain YJ1 can be used for microbial degradation of acephate.
引文
[1]贺敏.乙酰甲胺磷及其代谢物甲胺磷在小油菜和土壤中的降解及其水解、光解行为研究[D].长沙:湖南农业大学,2005.
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[12]解开治,徐培智,陈建生,等.乙酰甲胺磷降解菌株XP-3的分离鉴定及其生理特性研究[J].广东农业科学,2008,(8):79
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[14]侯进慧,韩芝洋,侯蕴鑫.小球藻共栖微生物种群多样性分析[J].生物技术,2016,26(4):386-390.
[15]王莉.甲胺磷降解菌株的分离鉴定、降解特性及菌株MAP-1的应用研究[D].南京:南京农业大学,2010.
[16]Ramya S L,Venkatesan T,Murthy K S,et al.Degradation of acephate by Enterobacter asburiae,Bacillus cereus and Pantoea agglomerans isolated from diamondback moth Plutella xylostella(L),a pest of cruciferous crops[J].Journal of Environmental Biology,2016,37(4):611-618.
[17]胡渤洋,王寿南,陈青君,等.4种常见农药的耐受真菌筛选、鉴定及降解作用初探[J].北京农学院学报,2017,32(1):7-14.
[18]龙家茹.乙酰甲胺磷高效降解菌的筛选与鉴定[J].安徽农业科学,2007,35(35):11504-11505.
[19]汤鸣强,尤民生.抗草甘膦酵母菌ZM-1的分离鉴定及其生长降解特性[J].微生物学通报,2010,37(9):1402-1409.
[20]石成春,郭养浩,王大奈,等.草甘膦曲霉生物降解的动力学研究[J].中国环境科学,2005,25(3):361-365.
[2]郑斐能,邓德峰.期待乙酰甲胺磷在我国成长为万吨级大品种[J].中国农药,2009,5(3):8-13.
[3]Chai L K,Wong M H,Mohdtahir N,et al.Degradation and mineralization kinetics of acephate in humid tropic soils of Malaysia[J].Chemosphere,2010,79(4):434.
[4]Loonen H,Lindgren F,Hansen B,et al.Prediction of biodegradability from chemical structure:Modeling of ready biodegradation test data[J].Environmental Toxicology&Chemistry,1999,18(8):1763-1768..
[5]Farag AT,Eweidah MH,El-Okazy AM.Reproductive toxicology of acephate in male mice[J].Reproductive Toxicology,2000,14(5):457.
[6]Kumar V,Upadhyay N,Kumar V,et al.A review on sample preparation and chromatographic determination of acephate and methamidophos in different samples[J].Arabian Journal of Chemistry,2015,8(5):624-631.
[7]Chai LK,Meehua W,Mohdtahir N,et al.Degradation and mineralization kinetics of acephate in humid tropic soils of Malaysia[J].Chemosphere,2010,79(4):434-440.
[8]杨轩,张威,李师翁,等.多环芳烃降解菌的分离鉴定及其生理特性研究[J].环境科学学报,2012,32(5):1033-1040.
[9]Bour M E.Microbial Degradation[M].Encyclopedia of Estuaries.Springer Netherlands,2016:433-433.
[10]于彩虹,张显涛,宋英男,等.2株乙酰甲胺磷降解菌的分离鉴定及降解特性研究[J].环境工程学报,2010,4(11):2623-2630.
[11]黎小军,廖上铁,陈奉英,等.甲胺磷降解细菌的筛选与降解特性研究[J].西南农业学报,2010,23(1):197-200.
[12]解开治,徐培智,陈建生,等.乙酰甲胺磷降解菌株XP-3的分离鉴定及其生理特性研究[J].广东农业科学,2008,(8):79
[13]侯进慧,刘彤,李同祥.产纤维素酶菌株Pantoea ananatis P5的筛选鉴定和活性研究[J].徐州工程学院学报:自然科学版,2013,28(4):80-84.
[14]侯进慧,韩芝洋,侯蕴鑫.小球藻共栖微生物种群多样性分析[J].生物技术,2016,26(4):386-390.
[15]王莉.甲胺磷降解菌株的分离鉴定、降解特性及菌株MAP-1的应用研究[D].南京:南京农业大学,2010.
[16]Ramya S L,Venkatesan T,Murthy K S,et al.Degradation of acephate by Enterobacter asburiae,Bacillus cereus and Pantoea agglomerans isolated from diamondback moth Plutella xylostella(L),a pest of cruciferous crops[J].Journal of Environmental Biology,2016,37(4):611-618.
[17]胡渤洋,王寿南,陈青君,等.4种常见农药的耐受真菌筛选、鉴定及降解作用初探[J].北京农学院学报,2017,32(1):7-14.
[18]龙家茹.乙酰甲胺磷高效降解菌的筛选与鉴定[J].安徽农业科学,2007,35(35):11504-11505.
[19]汤鸣强,尤民生.抗草甘膦酵母菌ZM-1的分离鉴定及其生长降解特性[J].微生物学通报,2010,37(9):1402-1409.
[20]石成春,郭养浩,王大奈,等.草甘膦曲霉生物降解的动力学研究[J].中国环境科学,2005,25(3):361-365.