血必净注射液通过调控p38 MAPK/NF-κB通路对百草枯中毒大鼠急性肺损伤保护作用的研究
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摘要
目的观察血必净注射液通过调控p38 MAPK/NF-κB通路对百草枯中毒大鼠急性肺损伤的保护作用。方法选取SPF级Wistar雄性大鼠,分为5组,除正常组外,其他各组大鼠一次性灌胃百草枯(PQ)溶液染毒。染毒1 h后血必净低、中、高剂量组大鼠分别经尾巴静脉注射含生药0.43、0.86、1.72 g/kg的血必净注射液2 mL,模型组和正常组大鼠注射等剂量0.9%氯化钠注射液。观察大鼠饮食、活动、病理状况,肺组织Bax、Bcl-2和p38-MAPK蛋白及NF-κB及IκB mRNA相对表达量。结果与正常组相比,模型组大鼠肺组织内Bax及Bcl-2蛋白表达上升;与模型组相比,血必净中、高剂量组大鼠肺组织内Bax蛋白表达下降,Bcl-2蛋白表达上升,差异有统计学意义(P <0.05);与正常组相比,模型组大鼠NF-κB m RNA、p38-MAPK蛋白表达上升,IκB mRNA表达下降;和模型组相比,血必净中、高剂量组大鼠NF-κB m RNA、p38-MAPK蛋白表达下降,IκB mRNA表达上升,差异均有统计学意义(P <0.05)。结论血必净注射液通过调控p38 MAPK/NF-κB通路,降低PQ中毒大鼠炎症反应程度,对受损肺组织起到保护作用。
Objective: To analyze the protective effect of Xuebijing Injection on acute lung injury induced by paraquat poisoning in rats by regulating p38 MAPK/NF-κB pathway. Methods: SPF-class Wistar male rats were divided into five groups. Except the normal group,rats in other groups were exposed to paraquat(PQ) solution once. One hour after the exposure,the rats in the low,medium and high doses of Xuebijing Injection groups were injected intravenously with 2 ml of Xuebijing Injection,and the rats in the model group and the normal group were injected with the same amount of normal saline. The diet,activity and pathological conditions of rats were observed. The relative expressions of Bax,Bcl-2 and p38-MAPK proteins,NF-κB and IB m RNA in lung tissues were observed. Results: Compared with the normal group,the expression of Bax and Bcl-2 protein in lung tissue of the model group rats increased. Compared with the model group,the expression of Bax protein in the middle and high dose groups of Xuebijing Injection decreased,and the expression of Bcl-2 protein increased,with statistical significance(P < 0.05);compared with the normal group,the expression of NF-κB mRNA and p38-MAPK protein in the model group increased,and the expression of IκB mRNA decreased. Compared with the model group,the expressions of NF-κB mRNA and p38-MAPK protein in Xuebijing Injection medium and high dose group were decreased,and the expression of IκB mRNA was increased,with statistical significance(P < 0.05).Conclusion: Xuebijing Injection can reduce the degree of inflammatory reaction in rats with PQ poisoning by regulating p38-MAPK/NF-κB pathway,and protect the damaged lung tissue.
Objective: To analyze the protective effect of Xuebijing Injection on acute lung injury induced by paraquat poisoning in rats by regulating p38 MAPK/NF-κB pathway. Methods: SPF-class Wistar male rats were divided into five groups. Except the normal group,rats in other groups were exposed to paraquat(PQ) solution once. One hour after the exposure,the rats in the low,medium and high doses of Xuebijing Injection groups were injected intravenously with 2 ml of Xuebijing Injection,and the rats in the model group and the normal group were injected with the same amount of normal saline. The diet,activity and pathological conditions of rats were observed. The relative expressions of Bax,Bcl-2 and p38-MAPK proteins,NF-κB and IB m RNA in lung tissues were observed. Results: Compared with the normal group,the expression of Bax and Bcl-2 protein in lung tissue of the model group rats increased. Compared with the model group,the expression of Bax protein in the middle and high dose groups of Xuebijing Injection decreased,and the expression of Bcl-2 protein increased,with statistical significance(P < 0.05);compared with the normal group,the expression of NF-κB mRNA and p38-MAPK protein in the model group increased,and the expression of IκB mRNA decreased. Compared with the model group,the expressions of NF-κB mRNA and p38-MAPK protein in Xuebijing Injection medium and high dose group were decreased,and the expression of IκB mRNA was increased,with statistical significance(P < 0.05).Conclusion: Xuebijing Injection can reduce the degree of inflammatory reaction in rats with PQ poisoning by regulating p38-MAPK/NF-κB pathway,and protect the damaged lung tissue.
引文
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[10]赵光举,李声琴,洪广亮,等.白藜芦醇对百草枯中毒小鼠肺损伤的干预及机制[J].中华危重病急救医学,2016,3(1):33-37.
[11]Xiong LL,Tan Y,Ma HY,et al.Administration of SB239063,a potent p38 MAPK inhibitor,alleviates acute lung injury induced by intestinal ischemia reperfusion in rats associated with AQP4 downregulation[J].International Immunopharmacology,2016,38(16):54-60.
[12]曹玉芳,王洪武,苏醒,等.二烯丙基硫化物对百草枯中毒急性肺损伤大鼠的抗氧化作用[J].中国药理学与毒理学杂志,2016,30(5):526-531.
[13]何谦,熊骥,曹钰,等.EGCG通过减少线粒体DNA水平减轻百草枯导致急性肺损伤的实验研究[J].西部医学,2016,28(6):759-762.
[14]曾诚,方辰,张剑锋,等.百草枯诱导急性肺损伤大鼠和肽素水平变化及甘草酸二铵对其影响[J].中国急救医学,2016,36(12):1111-1115.
[15]赵光举,李声琴,洪广亮,等.白藜芦醇对百草枯中毒小鼠肺损伤的干预及机制[J].中华危重病急救医学,2016,3(1):33-37.
[2]林飞,王甜甜,何义,等.P38 MAPK信号通路在急性肺损伤中作用的研究进展[J].重庆医学,2017,14(35):5023-5025.
[3]索冬卫,陈炅,孙秋虹,等.消退素D1对百草枯致急性肺损伤大鼠肺泡表面活性物质与巨噬细胞的影响[J].中国现代医学杂志,2016,5(3):23-26.
[4]武国艳,温宇英,白祥琰,等.硫辛酸通过Nrf2-ARE信号通路对百草枯中毒大鼠急性肺损伤保护作用的研究[J].中华急诊医学杂志,2017,26(7):94-98.
[5]范红斌,陈俊良,杨欣颖,等.亚甲蓝对百草枯所致大鼠急性肺损伤的抗氧化作用[J].卫生研究,2016,45(6):968-972.
[6]Xiong LL,Tan Y,Ma HY,et al.Administration of SB239063,a potent p38 MAPK inhibitor,alleviates acute lung injury induced by intestinal ischemia reperfusion in rats associated with AQP4 downregulation[J].International Immunopharmacology,2016,38(16):54-60.
[7]曹玉芳,王洪武,苏醒,等.二烯丙基硫化物对百草枯中毒急性肺损伤大鼠的抗氧化作用[J].中国药理学与毒理学杂志,2016,30(5):526-531.
[8]何谦,熊骥,曹钰,等.EGCG通过减少线粒体DNA水平减轻百草枯导致急性肺损伤的实验研究[J].西部医学,2016,28(6):759-762.
[9]曾诚,方辰,张剑锋,等.百草枯诱导急性肺损伤大鼠和肽素水平变化及甘草酸二铵对其影响[J].中国急救医学,2016,36(12):1111-1115.
[10]赵光举,李声琴,洪广亮,等.白藜芦醇对百草枯中毒小鼠肺损伤的干预及机制[J].中华危重病急救医学,2016,3(1):33-37.
[11]Xiong LL,Tan Y,Ma HY,et al.Administration of SB239063,a potent p38 MAPK inhibitor,alleviates acute lung injury induced by intestinal ischemia reperfusion in rats associated with AQP4 downregulation[J].International Immunopharmacology,2016,38(16):54-60.
[12]曹玉芳,王洪武,苏醒,等.二烯丙基硫化物对百草枯中毒急性肺损伤大鼠的抗氧化作用[J].中国药理学与毒理学杂志,2016,30(5):526-531.
[13]何谦,熊骥,曹钰,等.EGCG通过减少线粒体DNA水平减轻百草枯导致急性肺损伤的实验研究[J].西部医学,2016,28(6):759-762.
[14]曾诚,方辰,张剑锋,等.百草枯诱导急性肺损伤大鼠和肽素水平变化及甘草酸二铵对其影响[J].中国急救医学,2016,36(12):1111-1115.
[15]赵光举,李声琴,洪广亮,等.白藜芦醇对百草枯中毒小鼠肺损伤的干预及机制[J].中华危重病急救医学,2016,3(1):33-37.