黄芪多糖对实验性牙周炎骨吸收的影响
|
摘要
目的分析黄芪多糖对实验性牙周炎大鼠模型骨吸收的影响,评估黄芪多糖对牙槽骨炎性吸收的保护作用。方法SD大鼠随机分为对照组、模型组、黄芪多糖(100、200、500 mg/kg)组。除对照组外,其余各组大鼠采用引菌法制备右上磨牙牙周炎模型,黄芪多糖组分别ig给予黄芪多糖100、200、500mg/kg,对照组和模型组ig给予等量生理盐水。4周后处死大鼠,测量右上牙槽骨吸收状况及血清中白细胞介素-1β(IL-1β)、IL-6、肿瘤坏死因子-α(TNF-α)、核因子-κB受体活化因子配体(RANKL)、骨保护素(OPG)水平,同时测量血清总抗氧化态(TOS)、总还原态(TAS)水平,并计算氧化应激指数(OSI)。结果随黄芪多糖剂量的增加,大鼠牙槽骨吸收量、TOS、OSI明显下降,TAS、RANKL、RANKL/OPG明显上升,差异显著(P<0.05);各组IL-1β、IL-6、TNF-α、OPG水平差异无显著性(P>0.05)。结论黄芪多糖可通过下调OSI及RANKL/OPG抑制牙周炎性骨吸收。
Objective To analyze the effect of astragalus polysacharin(APC) on bone resorption in experimental periodontitis,and evaluate its protective function of inflammatory resorption of alveolar bone in periodontits.Methods SD rats were randomly divided into five equal groups:contorol group,model group,and APC low [LD:100 mg/(kg·d)],medium [MD:200 mg/(kg·d)],and high dose [HD:500 mg/(kg·d)] treatment groups.The periodontitis models were established through Porphyromonas gingivalis attracting.APC [LD:100 mg/(kg·d);MD:200 mg/(kg·d);HD:500 mg/(kg·d)] gavage was given to treatment groups,and the same amount of normal saline was given to control and model groups.The rats executed after four weeks,their CEJ-ABC distance(CAD) and expression of IL-1β,IL-6,TNF-α,TOS,TAS,RANKL,OPG in their serum was evaluated,and the oxidative stress index(OSI) was calculated.Results As APC amount increased,CAD,TOS,and OSI levels were declined significantly;While TAS,RANKL,RANKL/OPG levels were improved significantly;There was no significantly change on IL-1β,IL-6,TNF-α,and OPG levesl among groups.Conclusion APC prevents alveolar bone from oxidative stress and inflammatory damage by down-regulating OSI and RANKL/OPG.
Objective To analyze the effect of astragalus polysacharin(APC) on bone resorption in experimental periodontitis,and evaluate its protective function of inflammatory resorption of alveolar bone in periodontits.Methods SD rats were randomly divided into five equal groups:contorol group,model group,and APC low [LD:100 mg/(kg·d)],medium [MD:200 mg/(kg·d)],and high dose [HD:500 mg/(kg·d)] treatment groups.The periodontitis models were established through Porphyromonas gingivalis attracting.APC [LD:100 mg/(kg·d);MD:200 mg/(kg·d);HD:500 mg/(kg·d)] gavage was given to treatment groups,and the same amount of normal saline was given to control and model groups.The rats executed after four weeks,their CEJ-ABC distance(CAD) and expression of IL-1β,IL-6,TNF-α,TOS,TAS,RANKL,OPG in their serum was evaluated,and the oxidative stress index(OSI) was calculated.Results As APC amount increased,CAD,TOS,and OSI levels were declined significantly;While TAS,RANKL,RANKL/OPG levels were improved significantly;There was no significantly change on IL-1β,IL-6,TNF-α,and OPG levesl among groups.Conclusion APC prevents alveolar bone from oxidative stress and inflammatory damage by down-regulating OSI and RANKL/OPG.
引文
[1]Hajishengallis G,Moutsopoulos N M.Role of bacteria in leukocyte adhesion deficiency-associated periodontitis[J].Microb Pathog,2016,94(5):21-26.
[2]Yu Y H,Chasman D I,Buring J E,et al.Cardiovascular risks associated with incident and prevalent periodontal disease[J].J Clin Periodontol,2015,42(1):21-28.
[3]李珍一,杨关林,闫承慧,等.黄芪多糖应用的相关研究进展[J].辽宁中医杂志,2016,43(7):1553-1555.
[4]张莹,王蕾,杜梦楠.注射用黄芪多糖治疗肿瘤的临床研究进展[J].药物评价研究,2016,39(6):1092-1094.
[5]刘豫蓉,张朝良,孔祥丽,等.黄芪多糖对成骨细胞增殖分化和结构的影响[J].国际口腔医学杂志,2010,37(2):133-137.
[6]伍志伟,刘丹,薛娜,等.黄芪多糖对低氧环境中BMSCs成骨分化的影响[J].中国细胞生物学学报,2017,39(6):732-738.
[7]刘珂,邱炳勋,邹利,等.黄芪及其有效成分对内皮细胞及其连接的保护作用及机制研究进展[J].中草药,2016,47(21):3912-3917.
[8]Pierrefite-Carle V,Santucci-Darmanin S,Breuil V,et al.Autophagy in bone:Self-eating to stay in balance[J].Ageing Res Rev,2015,24(2):206-217.
[9]Hienz S A,Paliwal S,Ivanovski S.Mechanisms of bone resorption in periodontitis[J].J Immunol Res,2015,2015:615486.
[10]孔祥鹤,牛银波,王婷梅,等.黄芪多糖对大鼠原代成骨细胞的影响及其机制研究[J].中草药,2011,42(10):2065-2069.
[11]Moon J S,Kim S H,Oh S H,et al.Relaxin augments BMP-2-induced osteoblast differentiation and bone formation[J].J Bone Miner Res,2014,29(7):1586-1596.
[12]Zhang Y D,Zhao S C,Zhu Z S,et al.Cx43-and Smad-mediated TGF-β/BMP signaling pathway promotes cartilage differentiation of bone marrow mesenchymal stem cells and inhibits osteoblast differentiation[J].Cell Physiol Biochem,2017,42(4):1277-1293.
[13]Katakawa Y,Funaba M,Murakami M.Smad8/9 is regulated through the BMP pathway[J].J Cell Biochem,2016,117(8):1788-1796.
[14]Lu D G,Qu J N,Lei J L,et al.LPS-stimulated inflammation inhibits BMP-9-induced osteoblastic differentiation through crosstalk between BMP/MAPKand Smad signaling[J].Exp Cell Res,2016,341(1):54-60.
[15]Walsh M C,Choi Y.Biology of the RANKL-RANK-OPGsystem in immunity,bone,and beyond[J].Front Immunol,2014,doi:10.3389/fimmu.2014.00511.
[16]Takegami N,Akeda K,Yamada J,et al.RANK/RANKL/OPG system in the intervertebral disc[J].Arthritis Res Ther,2017,doi:org/10.1186/s13075-017-1332-y.
[17]Zhang H W,Peng L,Li W B,et al.The role of RANKL/RANK/OPG system in the canine model of hip periprosthetic infection osteolysis[J].Int J Artif Organs,2017,39(12):619-624.
[18]Huo J,Sun X.Effect of Astragalus polysaccharides on ovariectomy-induced osteoporosis in mice[J].Genet Mol Res,2016,doi:10.4238/gmr15049169.
[19]Oktay S,Chukkapalli S S,Rivera-Kweh M F,et al.Periodontitis in rats induces systemic oxidative stress that is controlled by bone-targeted antiresorptives[J].JPeriodontol,2015,86(1):137-145.
[20]Zhang D Y,Pan Y,Zhang C,et al.Wnt/β-catenin signaling induces the aging of mesenchymal stem cells through promoting the ROS production[J].Mol Cell Biochem,2013,374(1/2):13-20.
[21]Monturiol-Gross L,Flores-Díaz M,Pineda-Padilla M J,et al.Clostridium perfringens phospholipase C induced ROS production and cytotoxicity require PKC,MEK1and NFκB activation[J].PLoS One,2014,9(1):e86475.
[2]Yu Y H,Chasman D I,Buring J E,et al.Cardiovascular risks associated with incident and prevalent periodontal disease[J].J Clin Periodontol,2015,42(1):21-28.
[3]李珍一,杨关林,闫承慧,等.黄芪多糖应用的相关研究进展[J].辽宁中医杂志,2016,43(7):1553-1555.
[4]张莹,王蕾,杜梦楠.注射用黄芪多糖治疗肿瘤的临床研究进展[J].药物评价研究,2016,39(6):1092-1094.
[5]刘豫蓉,张朝良,孔祥丽,等.黄芪多糖对成骨细胞增殖分化和结构的影响[J].国际口腔医学杂志,2010,37(2):133-137.
[6]伍志伟,刘丹,薛娜,等.黄芪多糖对低氧环境中BMSCs成骨分化的影响[J].中国细胞生物学学报,2017,39(6):732-738.
[7]刘珂,邱炳勋,邹利,等.黄芪及其有效成分对内皮细胞及其连接的保护作用及机制研究进展[J].中草药,2016,47(21):3912-3917.
[8]Pierrefite-Carle V,Santucci-Darmanin S,Breuil V,et al.Autophagy in bone:Self-eating to stay in balance[J].Ageing Res Rev,2015,24(2):206-217.
[9]Hienz S A,Paliwal S,Ivanovski S.Mechanisms of bone resorption in periodontitis[J].J Immunol Res,2015,2015:615486.
[10]孔祥鹤,牛银波,王婷梅,等.黄芪多糖对大鼠原代成骨细胞的影响及其机制研究[J].中草药,2011,42(10):2065-2069.
[11]Moon J S,Kim S H,Oh S H,et al.Relaxin augments BMP-2-induced osteoblast differentiation and bone formation[J].J Bone Miner Res,2014,29(7):1586-1596.
[12]Zhang Y D,Zhao S C,Zhu Z S,et al.Cx43-and Smad-mediated TGF-β/BMP signaling pathway promotes cartilage differentiation of bone marrow mesenchymal stem cells and inhibits osteoblast differentiation[J].Cell Physiol Biochem,2017,42(4):1277-1293.
[13]Katakawa Y,Funaba M,Murakami M.Smad8/9 is regulated through the BMP pathway[J].J Cell Biochem,2016,117(8):1788-1796.
[14]Lu D G,Qu J N,Lei J L,et al.LPS-stimulated inflammation inhibits BMP-9-induced osteoblastic differentiation through crosstalk between BMP/MAPKand Smad signaling[J].Exp Cell Res,2016,341(1):54-60.
[15]Walsh M C,Choi Y.Biology of the RANKL-RANK-OPGsystem in immunity,bone,and beyond[J].Front Immunol,2014,doi:10.3389/fimmu.2014.00511.
[16]Takegami N,Akeda K,Yamada J,et al.RANK/RANKL/OPG system in the intervertebral disc[J].Arthritis Res Ther,2017,doi:org/10.1186/s13075-017-1332-y.
[17]Zhang H W,Peng L,Li W B,et al.The role of RANKL/RANK/OPG system in the canine model of hip periprosthetic infection osteolysis[J].Int J Artif Organs,2017,39(12):619-624.
[18]Huo J,Sun X.Effect of Astragalus polysaccharides on ovariectomy-induced osteoporosis in mice[J].Genet Mol Res,2016,doi:10.4238/gmr15049169.
[19]Oktay S,Chukkapalli S S,Rivera-Kweh M F,et al.Periodontitis in rats induces systemic oxidative stress that is controlled by bone-targeted antiresorptives[J].JPeriodontol,2015,86(1):137-145.
[20]Zhang D Y,Pan Y,Zhang C,et al.Wnt/β-catenin signaling induces the aging of mesenchymal stem cells through promoting the ROS production[J].Mol Cell Biochem,2013,374(1/2):13-20.
[21]Monturiol-Gross L,Flores-Díaz M,Pineda-Padilla M J,et al.Clostridium perfringens phospholipase C induced ROS production and cytotoxicity require PKC,MEK1and NFκB activation[J].PLoS One,2014,9(1):e86475.