白内障动物模型的研究进展
|
摘要
本文综述了近20年来国内外有关先天性、创伤性、人工诱导性和细胞水平白内障动物模型的建立方法、特点及相关机制研究,总结了各类型白内障动物模型构建的评价指标,提出了建立晶状体永生化细胞系,研究白内障发病过程晶状体细胞中基因表达水平的变化,以及建立与人类较相似的白内障树鼩模型的研究设想。
This paper summarized the method for establishing animal models of congenital,traumatic, artificially induced and cellular-level cataract that have been studied at domestic and abroad in nearly resent 20 years. The characteristics, evaluation criteria and related mechanisms of each model were also described. The paper also proposesed a method for studying the expression of gene in lens cells that in different state of cataract by established immortalized lens cell lines, and an assumption that setting up tree shrew model for cataract that are similar to human cataract.
This paper summarized the method for establishing animal models of congenital,traumatic, artificially induced and cellular-level cataract that have been studied at domestic and abroad in nearly resent 20 years. The characteristics, evaluation criteria and related mechanisms of each model were also described. The paper also proposesed a method for studying the expression of gene in lens cells that in different state of cataract by established immortalized lens cell lines, and an assumption that setting up tree shrew model for cataract that are similar to human cataract.
引文
[1]Shiels A,Hejtmancik JF.Molecular Genetics of Cataract[J].Prog Mol Biol Transl Sci,2015,134:203-218.
[2]World Health Organization.Global Data on Visual Impairments 2010[R].2012.
[3]韦茹倩,蓝正荣.白内障治疗的研究进展[J].当代医学,2013,19(7):14-16.
[4]Graw J,Jung M,L?ster J,et al.Mutation in the b A3/A1-crystallin encoding gene cryba1 causes a dominant cataract in the mouse[J].Genomics,1999,62(1):67-73
[5]Bu L,Yan S,Jin M,et al.The gamma S-crystallin gene is mutated in autosomal recessive cataract in mouse[J].Genomics,2002,80(1):38-44.
[6]Yuan L,Yao H,Xu Y,et al.CRISPR/Cas9-mediated mutation ofαA-Crystallin gene induces congenital cataracts in rabbits[J].Invest Ophthalmol Vis Sci,2017,58(6):BIO34-BIO41.
[7]Yamashita S,Furumoto K,Nobukiyo A,et al.Mapping of A gene responsible for cataract formation and its modifier in the UPL rat[J].Invest Ophthalmol Vis Sci,2002,43(10):3153-3159.
[8]Kondo T,Nagai H,Kawashima T,et al.Hereditary and histologic characteristics of the CF1/b cac mouse cataract model[J].Comp Med,2014,64(5):360-368.
[9]Kondo T,Nakamori T,Nagai H,et al.A novel spontaneous mutation of BCAR3 results in extrusion cataracts in CF#1mouse strain[J].Mamm Genome,2016,27(9-10):451-459.
[10]Wignes JA,Goldman JW,Weihl CC,et al.p62 expression and autophagy in alpha B-crystallin R120G mutant knock-in mouse model of hereditary cataract[J].Exp Eye Res,2013,115:263-273.
[11]Chauss D,Brennan LA,Bakina O,et al.IntegrinαVβ5-mediated removal of apoptotic cell debris by the eye lens and its inhibition by UV light exposure[J].J Biol Chem,2015,290(51):30253-30266.
[12]Liu WJ,Ye L,Huang WF,et al.p62 links the autophagy pathway and the ubiqutin-proteasome system upon ubiquitinated protein degradation[J].Cell Mol Biol Lett,2016,21:29.
[13]Moscat J,Karin M,Diaz-Meco MT.p62 in cancer:signaling adaptor beyond autophagy[J].Cell,2016,167(3):606-609.
[14]Chai P,Ni H,Zhang H,et al.The evolving functions of autophagy in ocular health:a double-edged sword[J].Int J Biol Sci,2016,12(11):1332-1340.
[15]Xiao W,Chen X,Li W,et al.Quantitative analysis of injuryinduced anterior subcapsular cataract in the mouse:a model of lens epithelial cells proliferation and epithelial-mesenchymal transition[J].Sci Rep,2015,5:8362.
[16]Kwon JW,Chung YW,Choi JA,et al.Comparison of postoperative corneal changes between dry eye and non-dry eye in a murine cataract surgery model[J].Int J Ophthalmol,2016,9(2):218-224.
[17]Galichanin K,Talebizadeh N,S?derberg P.Characterization of molecular mechanisms of in vivo UVR induced cataract[J].J Vis Exp,2012,(69):e4016.
[18]Mody VC,Kakar M,Sderberg PG,et al.High lenticular tolerance to ultraviolet radiation-B by pigmented guinea-pig;application of a safety limit strategy for UVR-induced cataract[J].Acta Ophthalmol,2012,90(3):226-230.
[19]Ishikawa Y,Hashizume K,Kishimoto S,et al.Effect of vitamin C depletion on UVR-B induced cataract in SMP30/GNL knockout mice[J].Exp Eye Res,2012,94(1):85-89.
[20]De Stefano I,Giardullo P,Tanno B,et al.Nonlinear radiationinduced cataract using the radiosensitive Ptch1(+/-)mouse model[J].Radiat Res,2016,186(3):315-321.
[21]De Stefano I,Tanno B,Giardullo P,et al.The Patched 1 tumorsuppressor gene protects the mouse lens from spontaneous and radiation-induced cataract[J].Am J Pathol,2015,185(1):85-95.
[22]Zhang J,Yan H,Lou MF,et al.Does oxidative stress play any role in diabetic cataract formation-Re-evaluation using a thioltransferase gene knockout mouse model[J].Exp Eye Res,2017,161:36-42.
[23]Ji L,Li C,Shen N,et al.A simple and stable galactosemic cataract model for rats[J].Int J Clin Exp Med,2015,8(8):12874-81.
[24]Patil MA,Suryanarayana P,Putcha UK,et al.Evaluation of neonatal streptozotocin induced diabetic rat model for the development of cataract[J].Oxid Med Cell Longev,2014:463264.
[25]Hao LN,He SZ,Shen YH,et al.Protective effects of puerarin on lens epithelial cells in rat diabetic cataract[J].Zhonghua Yan Ke Za Zhi,2011,47(4):320-326.
[26]Wang F,Ma J,Han F,et al.DL-3-n-butylphthalide delays the onset and progression of diabetic cataract by inhibiting oxidative stress in rat diabetic model[J].Sci Rep,2016,6:19396.
[27]Lee SM,Jeong EM,Jeong J,et al.Cysteamine prevents the development of lens opacity in a rat model of selenite-induced cataract[J].Invest Ophthalmol Vis Sci,2012,53(3):1452-1459.
[28]韩笑,王欣玲,吴迪,等.SUMO蛋白在体外培养的人晶状体上皮细胞内的表达及对氧化应激的调控作用[J].中国医科大学学报,2015,44(3):193-198.
[29]刘珍珍,李平华,陶树新,等.过氧化氢诱导体外损伤性白内障模型的构建及晶状体上皮细胞的凋亡[J].中国组织工程研究与临床康复,2011,15(28):5197-5200.
[30]Sampath S,Mc Lean LA,Buono C,et al.The use of rat lens explant cultures to study the mechanism of drug-induced cataractogenesis[J].Toxicol Sci,2012,126(1):128-39.
[31]Li G,Luna C,Navarro ID,et al.Resveratrol prevention of oxidative stress damage to lens epithelial cell cultures is mediated by forkhead box O activity[J].Invest Ophthalmol Vis Sci,2011,52(7):4395-4401.
[32]Du Y,Guo D,Wu Q,et al.Protective effects of appropriate Zn(2+)levels against UVB radiation-induced damage in human lens epithelial cells in vitro[J].J Biol Inorg Chem,2016,21(2):213-226.
[33]张栋彦,鲁建华,张文芳.高脂血症对大鼠半乳糖性白内障的影响[J].兰州大学学报:医学版,2010,36(1):17-19+23.
[34]刘会彬,李琪,李英卓,等.年龄相关性白内障血清微量元素与抗氧化酶的变化[J].河北医药,2015,37(7):1048-1050.
[35]祁磊,林媛,徐国兴,等.石决明对大鼠白内障晶状体中SOD和GSH及GSH-PX的影响[J].国际眼科杂志,2011,11(12):2085-2087.
[36]Kado M,Ishiko S,Yoshida A,et al.Lenticular autofluorescence in normal tree shrews[J].Ophthalmic Res,1996,28(3):160-164.
[2]World Health Organization.Global Data on Visual Impairments 2010[R].2012.
[3]韦茹倩,蓝正荣.白内障治疗的研究进展[J].当代医学,2013,19(7):14-16.
[4]Graw J,Jung M,L?ster J,et al.Mutation in the b A3/A1-crystallin encoding gene cryba1 causes a dominant cataract in the mouse[J].Genomics,1999,62(1):67-73
[5]Bu L,Yan S,Jin M,et al.The gamma S-crystallin gene is mutated in autosomal recessive cataract in mouse[J].Genomics,2002,80(1):38-44.
[6]Yuan L,Yao H,Xu Y,et al.CRISPR/Cas9-mediated mutation ofαA-Crystallin gene induces congenital cataracts in rabbits[J].Invest Ophthalmol Vis Sci,2017,58(6):BIO34-BIO41.
[7]Yamashita S,Furumoto K,Nobukiyo A,et al.Mapping of A gene responsible for cataract formation and its modifier in the UPL rat[J].Invest Ophthalmol Vis Sci,2002,43(10):3153-3159.
[8]Kondo T,Nagai H,Kawashima T,et al.Hereditary and histologic characteristics of the CF1/b cac mouse cataract model[J].Comp Med,2014,64(5):360-368.
[9]Kondo T,Nakamori T,Nagai H,et al.A novel spontaneous mutation of BCAR3 results in extrusion cataracts in CF#1mouse strain[J].Mamm Genome,2016,27(9-10):451-459.
[10]Wignes JA,Goldman JW,Weihl CC,et al.p62 expression and autophagy in alpha B-crystallin R120G mutant knock-in mouse model of hereditary cataract[J].Exp Eye Res,2013,115:263-273.
[11]Chauss D,Brennan LA,Bakina O,et al.IntegrinαVβ5-mediated removal of apoptotic cell debris by the eye lens and its inhibition by UV light exposure[J].J Biol Chem,2015,290(51):30253-30266.
[12]Liu WJ,Ye L,Huang WF,et al.p62 links the autophagy pathway and the ubiqutin-proteasome system upon ubiquitinated protein degradation[J].Cell Mol Biol Lett,2016,21:29.
[13]Moscat J,Karin M,Diaz-Meco MT.p62 in cancer:signaling adaptor beyond autophagy[J].Cell,2016,167(3):606-609.
[14]Chai P,Ni H,Zhang H,et al.The evolving functions of autophagy in ocular health:a double-edged sword[J].Int J Biol Sci,2016,12(11):1332-1340.
[15]Xiao W,Chen X,Li W,et al.Quantitative analysis of injuryinduced anterior subcapsular cataract in the mouse:a model of lens epithelial cells proliferation and epithelial-mesenchymal transition[J].Sci Rep,2015,5:8362.
[16]Kwon JW,Chung YW,Choi JA,et al.Comparison of postoperative corneal changes between dry eye and non-dry eye in a murine cataract surgery model[J].Int J Ophthalmol,2016,9(2):218-224.
[17]Galichanin K,Talebizadeh N,S?derberg P.Characterization of molecular mechanisms of in vivo UVR induced cataract[J].J Vis Exp,2012,(69):e4016.
[18]Mody VC,Kakar M,Sderberg PG,et al.High lenticular tolerance to ultraviolet radiation-B by pigmented guinea-pig;application of a safety limit strategy for UVR-induced cataract[J].Acta Ophthalmol,2012,90(3):226-230.
[19]Ishikawa Y,Hashizume K,Kishimoto S,et al.Effect of vitamin C depletion on UVR-B induced cataract in SMP30/GNL knockout mice[J].Exp Eye Res,2012,94(1):85-89.
[20]De Stefano I,Giardullo P,Tanno B,et al.Nonlinear radiationinduced cataract using the radiosensitive Ptch1(+/-)mouse model[J].Radiat Res,2016,186(3):315-321.
[21]De Stefano I,Tanno B,Giardullo P,et al.The Patched 1 tumorsuppressor gene protects the mouse lens from spontaneous and radiation-induced cataract[J].Am J Pathol,2015,185(1):85-95.
[22]Zhang J,Yan H,Lou MF,et al.Does oxidative stress play any role in diabetic cataract formation-Re-evaluation using a thioltransferase gene knockout mouse model[J].Exp Eye Res,2017,161:36-42.
[23]Ji L,Li C,Shen N,et al.A simple and stable galactosemic cataract model for rats[J].Int J Clin Exp Med,2015,8(8):12874-81.
[24]Patil MA,Suryanarayana P,Putcha UK,et al.Evaluation of neonatal streptozotocin induced diabetic rat model for the development of cataract[J].Oxid Med Cell Longev,2014:463264.
[25]Hao LN,He SZ,Shen YH,et al.Protective effects of puerarin on lens epithelial cells in rat diabetic cataract[J].Zhonghua Yan Ke Za Zhi,2011,47(4):320-326.
[26]Wang F,Ma J,Han F,et al.DL-3-n-butylphthalide delays the onset and progression of diabetic cataract by inhibiting oxidative stress in rat diabetic model[J].Sci Rep,2016,6:19396.
[27]Lee SM,Jeong EM,Jeong J,et al.Cysteamine prevents the development of lens opacity in a rat model of selenite-induced cataract[J].Invest Ophthalmol Vis Sci,2012,53(3):1452-1459.
[28]韩笑,王欣玲,吴迪,等.SUMO蛋白在体外培养的人晶状体上皮细胞内的表达及对氧化应激的调控作用[J].中国医科大学学报,2015,44(3):193-198.
[29]刘珍珍,李平华,陶树新,等.过氧化氢诱导体外损伤性白内障模型的构建及晶状体上皮细胞的凋亡[J].中国组织工程研究与临床康复,2011,15(28):5197-5200.
[30]Sampath S,Mc Lean LA,Buono C,et al.The use of rat lens explant cultures to study the mechanism of drug-induced cataractogenesis[J].Toxicol Sci,2012,126(1):128-39.
[31]Li G,Luna C,Navarro ID,et al.Resveratrol prevention of oxidative stress damage to lens epithelial cell cultures is mediated by forkhead box O activity[J].Invest Ophthalmol Vis Sci,2011,52(7):4395-4401.
[32]Du Y,Guo D,Wu Q,et al.Protective effects of appropriate Zn(2+)levels against UVB radiation-induced damage in human lens epithelial cells in vitro[J].J Biol Inorg Chem,2016,21(2):213-226.
[33]张栋彦,鲁建华,张文芳.高脂血症对大鼠半乳糖性白内障的影响[J].兰州大学学报:医学版,2010,36(1):17-19+23.
[34]刘会彬,李琪,李英卓,等.年龄相关性白内障血清微量元素与抗氧化酶的变化[J].河北医药,2015,37(7):1048-1050.
[35]祁磊,林媛,徐国兴,等.石决明对大鼠白内障晶状体中SOD和GSH及GSH-PX的影响[J].国际眼科杂志,2011,11(12):2085-2087.
[36]Kado M,Ishiko S,Yoshida A,et al.Lenticular autofluorescence in normal tree shrews[J].Ophthalmic Res,1996,28(3):160-164.