结构生物学研究热点
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摘要
<正>以蛋白质为代表的生物大分子是生命体内各种生理功能的主要执行者,而蛋白质的功能通常由其三维结构决定。因此,解析生物大分子的三维结构对理解其功能,进而理解其参与生命过程的机制,最终实现对生命过程的有效调控有着非常重要的意义。结构生物学是生物物理学的一个重要分支。该学科主要应用物
引文
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[13]QI S,PANG Y,HU Q,et al.Crystal structure of the Caenorhabditis elegans apoptosome reveals an octameric assembly of CED-4[J].Cell,2010,141(3):446-457.
[14]ZHANG P,WANG J,SHI Y.Structure and mechanism of the S component of a bacterial ECF transporter[J].Nature,2010,468(7324):717-720.
[15]ZHAN X,YAN C,ZHANG X,et al.Structure of a human catalytic step I spliceosome[J].Science,2018,359(6375):537-545.
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[17]QIU X,LEI Y,YANG P,et al.Structural basis for neutralization of Japanese encephalitis virus by two potent therapeutic antibodies[J].Nat Microbiol,2018,3(3):287-294.
[18]SHEN H,LI Z,JIANG Y,et al.Structural basis for the modulation of voltage-gated sodium channels by animal toxins[J].Science,2018,362(6412):eaau2596.
[19]WU J,YAN Z,LI Z,et al.Structure of the voltage-gated calcium channel Cav1.1 complex[J].Science,2015,350(6267):aad2395.
[20]LIU S,XU L,GUAN F,et al.Cryo-EM structure of the humanα5β3 GABAAreceptor[J].Cell Res,2018,28(9):958-961.
[2]HITE R K,MACKINNON R.Structural titration of Slo2.2,a Na+-dependent K+channel[J].Cell,2017,168(3):390-399.e11.
[3]RUAN Y,KAO K,LEFEBVRE S,et al.Structural titration of receptor ion channel GLIC gating by HS-AFM[J].Proc Natl Acad Sci U S A,2018,115(41):10333-10338.
[4]YANG F,CUI Y,WANG K,et al.Thermosensitive TRP channel pore turret is part of the temperature activation pathway[J].Proc Natl Acad Sci U S A,2010,107(15):7083-7088.
[5]YANG F,XIAO X,LEE B H,et al.The conformational wave in capsaicin activation of transient receptor potential vanilloid1 ion channel[J].Nat Commun,2018,9(1):2879.
[6]GAO Y,CAO E,JULIUS D,et al.TRPV1 structures in nanodiscs reveal mechanisms of ligand and lipid action[J].Nature,2016,534(7607):347-351.
[7]BECK M,BAUMEISTER W.Cryo-electron tomography:can it reveal the molecular sociology of cells in atomic detail?[J].Trends Cell Biol,2016,26(11):825-837.
[8]KUMAR A,KUHN L T,BALBACH J.In-cell NMR:analysis of protein-small molecule interactions,metabolic processes,and protein phosphorylation[J/OL].Int J Mol Sci,2019,20(2):pii:E378.
[9]SANTOS R,URSU O,GAULTON A,et al.A comprehensive map of molecular drug targets[J].Nat Rev Drug Discov,2017,16(1):19-34.
[10]CONGREVE M,OSWALD C,MARSHALL F H.Applying structure-based drug design approaches to allosteric modulators of GPCRs[J].Trends Pharmacol Sci,2017,38(9):837-847.
[11]WHITEHEAD T A,CHEVALIER A,SONG Y,et al.Optimization of affinity,specificity and function of designed influenza inhibitors using deep sequencing[J].Nat Biotechnol,2012,30(6):543-548.
[12]SILVA D A,YU S,ULGE U Y,et al.De novo design of potent and selective mimics of IL-2 and IL-15[J].Nature,2019,565(7738):186-191.
[13]QI S,PANG Y,HU Q,et al.Crystal structure of the Caenorhabditis elegans apoptosome reveals an octameric assembly of CED-4[J].Cell,2010,141(3):446-457.
[14]ZHANG P,WANG J,SHI Y.Structure and mechanism of the S component of a bacterial ECF transporter[J].Nature,2010,468(7324):717-720.
[15]ZHAN X,YAN C,ZHANG X,et al.Structure of a human catalytic step I spliceosome[J].Science,2018,359(6375):537-545.
[16]YUAN S,WANG J,ZHU D,et al.Cryo-EM structure of a herpesvirus capsid at 3.1?[J].Science,2018,360(6384).
[17]QIU X,LEI Y,YANG P,et al.Structural basis for neutralization of Japanese encephalitis virus by two potent therapeutic antibodies[J].Nat Microbiol,2018,3(3):287-294.
[18]SHEN H,LI Z,JIANG Y,et al.Structural basis for the modulation of voltage-gated sodium channels by animal toxins[J].Science,2018,362(6412):eaau2596.
[19]WU J,YAN Z,LI Z,et al.Structure of the voltage-gated calcium channel Cav1.1 complex[J].Science,2015,350(6267):aad2395.
[20]LIU S,XU L,GUAN F,et al.Cryo-EM structure of the humanα5β3 GABAAreceptor[J].Cell Res,2018,28(9):958-961.