加味四君子对宫颈癌大鼠模型癌组织中Caspase-3、Caspase-7、Survivin表达的干预作用
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摘要
目的:观察加味四君子汤对宫颈癌模型癌组织Caspase-3、Caspase-7、Survivin表达的影响,以探明加味四君子汤治疗宫颈癌的机制。方法:将30只Wistar雌性大鼠随机分为空白组、模型组及干预组,其中空白组10只,模型组及干预组各9只。其中空白组大鼠不接受造模及干预,模型组大鼠仅接受模型制备,干预组大鼠接受模型制备及加味四君子汤灌胃,连续治疗8周,疗程结束后比较各组宫颈癌病灶大小,同时用免疫组化法检测各组宫颈癌组织Caspase-3、Caspase-7、Survivin的水平表达变化。结果:1)空白组未出现宫颈癌病灶,模型组病灶体积(1 573. 48±11. 24) mm3,干预组病灶体积(683. 23±4. 81) mm3,各组宫颈癌病灶大小差异有统计学意义(P <0. 05),干预组明显较模型组小。2) 2组接受造模术大鼠Caspase-3、Caspase-7浓度均有不同程度下调,Survivin表达升高,其中干预组大鼠经过为期8周的干预后Caspase-3、Caspase-7浓度上调,Survivin浓度下降,干预组改善明显优于模型组,差异有统计学意义(P <0. 05)。3)经过相关分析显示,癌灶体积与Caspase-3、Caspase-7呈负相关关系,与Survivin有正相关关系。结论:加味四君子可明显缩小宫颈癌病灶体积,其作用机制可能与调节Caspase-3、Caspase-7、Survivin表达有关。
Objective: To observe the impact of Modified Sijunzi Decoction on the expression of Caspase-3,Caspase-7,Survivin in the cervical cancer model tissue,and to explore the mechanism of modified Sijunzi Decoction in the treatment of cerical cancer.Methods: A total of 30 Wistar female rats were randomly divided into blank group,model group and intervention group,10 in each group. The blank group rats was not modeled and intervened,and the model group only accepted model preparation,the intervention group accepted model preparation and modified Sijunzi Decoction lavage,which continued for 8 weeks. A comparison was made of the cervical cancer lesion size of these groups after treatment,at the same time the immunohistochemical method was used to detect the expression level changes of Caspase-3,Caspase-7 and Survivin in each cervical cancer tissue. Results: 1) The blank group did not have cervical lesion,the cervical lesion volume of the model group were( 1573. 48 ± 11. 24) mm3,the cervical lesion volume of the intervention group were( 683. 23 ± 4. 81) mm3. The difference in cervical lesion size had statistical significance( P < 0. 05),and the intervention group was significantly smaller compared with the model group. 2) The concentration of Caspase-3 and Caspase-7 in 2 groups was lower in different extent,and the expression of Survivin increased. After 8 weeks of intervention,the concentration of Caspase 3 and Caspase-7 in the intervention group increased and the concentration of Survivin decreased. The improvement of the intervention group was obviously better than that of the model group,and the difference was statistically significant( P < 0. 05). 3) The correlation analysis showed that cancer volume were negatively related to Caspase-3 and Caspase-7,but with positive correlation to Survivin. Conclusion: Modified Sijunzi Decoction can obviously reduce cervical lesion volume,and its mechanism may be related to regulation of the expression of Caspase-3,Caspase-7 and Survivin.
Objective: To observe the impact of Modified Sijunzi Decoction on the expression of Caspase-3,Caspase-7,Survivin in the cervical cancer model tissue,and to explore the mechanism of modified Sijunzi Decoction in the treatment of cerical cancer.Methods: A total of 30 Wistar female rats were randomly divided into blank group,model group and intervention group,10 in each group. The blank group rats was not modeled and intervened,and the model group only accepted model preparation,the intervention group accepted model preparation and modified Sijunzi Decoction lavage,which continued for 8 weeks. A comparison was made of the cervical cancer lesion size of these groups after treatment,at the same time the immunohistochemical method was used to detect the expression level changes of Caspase-3,Caspase-7 and Survivin in each cervical cancer tissue. Results: 1) The blank group did not have cervical lesion,the cervical lesion volume of the model group were( 1573. 48 ± 11. 24) mm3,the cervical lesion volume of the intervention group were( 683. 23 ± 4. 81) mm3. The difference in cervical lesion size had statistical significance( P < 0. 05),and the intervention group was significantly smaller compared with the model group. 2) The concentration of Caspase-3 and Caspase-7 in 2 groups was lower in different extent,and the expression of Survivin increased. After 8 weeks of intervention,the concentration of Caspase 3 and Caspase-7 in the intervention group increased and the concentration of Survivin decreased. The improvement of the intervention group was obviously better than that of the model group,and the difference was statistically significant( P < 0. 05). 3) The correlation analysis showed that cancer volume were negatively related to Caspase-3 and Caspase-7,but with positive correlation to Survivin. Conclusion: Modified Sijunzi Decoction can obviously reduce cervical lesion volume,and its mechanism may be related to regulation of the expression of Caspase-3,Caspase-7 and Survivin.
引文
[1]刘倩,王玮.浅谈宫颈癌临床治疗新进展[J].实用医学杂志,2018,34(1):5-7.
[2]郑玲英,赵银鹰.四君子汤抗肿瘤药理研究[J].实用中西医结合临床,2010,10(6):93-94.
[3]林乐铭.加味四君子汤治疗大肠癌化疗后不良反应的临床研究[D].广州:广州中医药大学,2014.
[4]禹雯琦,孙珏,周诣,杜锦芳,石晓兰.四君子汤及加味方治疗恶性肿瘤的临床及基础研究进展[J].上海中医药大学学报,2017,31(5):95-101.
[5]高岿然,史铁梅,邸晶,等.宫颈癌移植瘤大鼠动物模型的建立[J].中国组织工程研究与临床康复,2008,12(28):5494-5498.
[6]崔英,胥爱辉,蔡文娥,等.HPV致官颈癌的分子机制研究[J].现代胖瘤学,2008,16(1):143-145.
[7]简小兰,蒋益兰,曾普华.蒋益兰教授论治宫颈癌学术经验拾菁[J].湖南中医药大学学报,2015,35(7):27-29.
[8]冯骁,田聆,黄倩.Caspase-3基因表达调控研究进展[J].生命科学,2014,26(9):936-942.
[9]杨斌,陈赛英,史佃云,等.宫颈癌术前介入化疗前后组织中Survivin、Caspase-3和Caspase-7的表达变化及意义[J].临床肿瘤学杂志,2009,14(1):39-42.
[10]夏丽,薛秀珍.NF-κBp65,c-IAP2,Caspase-3在宫颈癌中的表达及意义[J].现代妇产科进展,2011,20(5):353-357.
[11]赵亚丽,王聪.苦参消癥汤对宫颈癌大鼠模型癌组织中Caspase-3、Caspase-7、Survivin表达的干预作用[J].陕西中医,2018,39(5):550-552,560.
[12]鲁艳明,孟丽荣,银铎,等.沉默survivin基因表达对人宫颈癌hela细胞恶性行为的影响[J].解剖科学进展,2011,17(3):259-263.
[13]高艳,曹建平,全桂凤,等.苦参碱对大鼠宫颈癌组织中Survivin、Caspase-3和Caspase-7表达的影响[J].现代生物医学进展,2015,15(23):4471-4473.
[14]Pereira C,Lopes-Rodrigues V,Coutinho I,et al.Potential small-molecule activators of caspase-7 identified using yeast-based caspase-3and-7 screening assays[J].Eur J Pharm Sci,2014,54:8-16.
[15]Tran LT,Tran LT,Bui TC,et al.Risk factors for high-risk and multitype Human Papillomavirus infections among women in Ho Chi Minh City,Vietnam:a cross-sectional study[J].BMC WomensHealth,2015,15(1):172.
[16]Mwaka AD,Okello ES,Wabinga H,et al.Symptomatic presentation with cervical cancer in Uganda:a qualitative study assessing the pathways to diagnosis in a low-income country[J].BMC Womens Health,2015,15:15.
[17]Shiju C,Arish D,Bhuvanesh N,et al.Synthesis,characterization,and biological evaluation of Schiff base-platinum(II)complexes[J].Spectrochim Acta A Mol Biomol Spectrosc,2015,145:213-222.
[18]Dang YP,Yuan XY,Tian R,et al.Curcumin improves the paclitaxel-induced apoptosis of HPV-positive human cervical cancer cells via the NF-κB-p53-caspase-3 pathway[J].Exp Ther Med,2015,9(4):1470-1476.
[19]Naumovic T,Miljus D,Djoric M,et al.Mortality from cervical cancer in Serbia in the period 1991-2011[J].J BUON,2015,20(1):231-234.
[20]Chen CH,Chung CY,Wang LH,et al.Risk of cancer among HIV-infected patients from a population-based nested case-control study:implications for cancer prevention[J].BMC Cancer,2015,15:133.
[2]郑玲英,赵银鹰.四君子汤抗肿瘤药理研究[J].实用中西医结合临床,2010,10(6):93-94.
[3]林乐铭.加味四君子汤治疗大肠癌化疗后不良反应的临床研究[D].广州:广州中医药大学,2014.
[4]禹雯琦,孙珏,周诣,杜锦芳,石晓兰.四君子汤及加味方治疗恶性肿瘤的临床及基础研究进展[J].上海中医药大学学报,2017,31(5):95-101.
[5]高岿然,史铁梅,邸晶,等.宫颈癌移植瘤大鼠动物模型的建立[J].中国组织工程研究与临床康复,2008,12(28):5494-5498.
[6]崔英,胥爱辉,蔡文娥,等.HPV致官颈癌的分子机制研究[J].现代胖瘤学,2008,16(1):143-145.
[7]简小兰,蒋益兰,曾普华.蒋益兰教授论治宫颈癌学术经验拾菁[J].湖南中医药大学学报,2015,35(7):27-29.
[8]冯骁,田聆,黄倩.Caspase-3基因表达调控研究进展[J].生命科学,2014,26(9):936-942.
[9]杨斌,陈赛英,史佃云,等.宫颈癌术前介入化疗前后组织中Survivin、Caspase-3和Caspase-7的表达变化及意义[J].临床肿瘤学杂志,2009,14(1):39-42.
[10]夏丽,薛秀珍.NF-κBp65,c-IAP2,Caspase-3在宫颈癌中的表达及意义[J].现代妇产科进展,2011,20(5):353-357.
[11]赵亚丽,王聪.苦参消癥汤对宫颈癌大鼠模型癌组织中Caspase-3、Caspase-7、Survivin表达的干预作用[J].陕西中医,2018,39(5):550-552,560.
[12]鲁艳明,孟丽荣,银铎,等.沉默survivin基因表达对人宫颈癌hela细胞恶性行为的影响[J].解剖科学进展,2011,17(3):259-263.
[13]高艳,曹建平,全桂凤,等.苦参碱对大鼠宫颈癌组织中Survivin、Caspase-3和Caspase-7表达的影响[J].现代生物医学进展,2015,15(23):4471-4473.
[14]Pereira C,Lopes-Rodrigues V,Coutinho I,et al.Potential small-molecule activators of caspase-7 identified using yeast-based caspase-3and-7 screening assays[J].Eur J Pharm Sci,2014,54:8-16.
[15]Tran LT,Tran LT,Bui TC,et al.Risk factors for high-risk and multitype Human Papillomavirus infections among women in Ho Chi Minh City,Vietnam:a cross-sectional study[J].BMC WomensHealth,2015,15(1):172.
[16]Mwaka AD,Okello ES,Wabinga H,et al.Symptomatic presentation with cervical cancer in Uganda:a qualitative study assessing the pathways to diagnosis in a low-income country[J].BMC Womens Health,2015,15:15.
[17]Shiju C,Arish D,Bhuvanesh N,et al.Synthesis,characterization,and biological evaluation of Schiff base-platinum(II)complexes[J].Spectrochim Acta A Mol Biomol Spectrosc,2015,145:213-222.
[18]Dang YP,Yuan XY,Tian R,et al.Curcumin improves the paclitaxel-induced apoptosis of HPV-positive human cervical cancer cells via the NF-κB-p53-caspase-3 pathway[J].Exp Ther Med,2015,9(4):1470-1476.
[19]Naumovic T,Miljus D,Djoric M,et al.Mortality from cervical cancer in Serbia in the period 1991-2011[J].J BUON,2015,20(1):231-234.
[20]Chen CH,Chung CY,Wang LH,et al.Risk of cancer among HIV-infected patients from a population-based nested case-control study:implications for cancer prevention[J].BMC Cancer,2015,15:133.