血清IL-15、IL-17测定在判断肺炎支原体肺炎严重程度中的作用
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摘要
目的:探讨肺炎支原体肺炎(MPP)患儿血清白细胞介素(IL)-15、IL-17在判断病情严重程度中的作用。方法:选取2015年1月至2016年12月陕西省咸阳市第一人民医院儿科收治的MPP患儿265例,根据病情严重程度分为MPP组228例,重症MPP(SMPP)组37例,以健康儿童100例为对照组,采用酶联免疫吸附试验(ELISA)法检测并比较3组血清IL-15、IL-17水平。结果:MPP组和SMPP组急性期血清IL-15、IL-17水平均高于对照组(均P<0.05);MPP组恢复期血清IL-15水平高于对照组(P<0.05);SMPP组急性期血清IL-15、IL-17水平均高于MPP组(均P<0.05);SMPP组恢复期血清IL-17水平高于对照组(P<0.05)。Pearson相关分析显示,MPP严重程度与血清IL-15、IL-17水平呈正相关关系(r=0.34,0.27,P<0.05)。结论:MPP患儿血清IL-15、IL-17水平高于正常儿童,并随着病情加重而增高。
Objective:To investigate the role of serum interleukin(IL)-15 and IL-17 levels in evaluating the severity of Mycoplasma pneumoniae pneumonia(MPP).Methods:A total of 265 children diagnosed with MPP in our hospital from January 2015 to December 2016 were selected,and were randomly divided into MPP group(n=228)and severe MPP(SMPP)group(n=37)according to the severity of the disease.One hundred healthy children were selected as a control group.The levels of IL-15 and IL-17 were detected by enzyme linked immunosorbent assay(ELISA).Results:The levels of IL-15 and IL-17 in MPP and SMPP groups were significantly higher than those in the control group(P<0.05).The IL-15 level of MPP patients during convalescence was higher than that of controls(P<0.05).Serum levels of IL-15 and IL-17 at the acute phase in SMPP group were significantly higher than those in the MPP group(P<0.05).The IL-17 level of SMPP patients at the recovery phase was significantly higher than those of controls(P<0.05).Pearson correlation analysis showed that the severity of MPP was positively correlated with serum IL-15 and IL-17 levels(P<0.05).Conclusion:The serum levels of IL-15 and IL-17 in MPP children were significantly higher than those of healthy children,and were increased during disease progression.
Objective:To investigate the role of serum interleukin(IL)-15 and IL-17 levels in evaluating the severity of Mycoplasma pneumoniae pneumonia(MPP).Methods:A total of 265 children diagnosed with MPP in our hospital from January 2015 to December 2016 were selected,and were randomly divided into MPP group(n=228)and severe MPP(SMPP)group(n=37)according to the severity of the disease.One hundred healthy children were selected as a control group.The levels of IL-15 and IL-17 were detected by enzyme linked immunosorbent assay(ELISA).Results:The levels of IL-15 and IL-17 in MPP and SMPP groups were significantly higher than those in the control group(P<0.05).The IL-15 level of MPP patients during convalescence was higher than that of controls(P<0.05).Serum levels of IL-15 and IL-17 at the acute phase in SMPP group were significantly higher than those in the MPP group(P<0.05).The IL-17 level of SMPP patients at the recovery phase was significantly higher than those of controls(P<0.05).Pearson correlation analysis showed that the severity of MPP was positively correlated with serum IL-15 and IL-17 levels(P<0.05).Conclusion:The serum levels of IL-15 and IL-17 in MPP children were significantly higher than those of healthy children,and were increased during disease progression.
引文
[1]IZUMIKAWA K,IZUMIKAWA K,TAKAZONO T,et al.Clinical features,risk factors and treatment of fulminant Mycoplasma pneumoniae pneumonia:a review of the Japanese literature[J].Infect Chemother,2014,20(3):181-185.
[2]王有鹏,景伟超,谭杰军.儿童支原体肺炎IFN-γ、IL-4水平中西医研究现状[J].中医药学报,2014,42(4):133-135.
[3]KAWAI Y,MIYASHITA N,KUBO M,et al.Therapeutic efficacy of macrolides,minocycline,and tosufloxacin against macrolide-resistant Mycoplasma pneumoniae pneumonia in pediatric patients[J].Antimicrob A-gents Chemother,2013,57(5):2252-2258.
[4]ZHOU Y,ZHANG Y,SHENG Y,et al.More complications occur in macrolide-resistant than in macrolidesensitive Mycoplasma pneumoniae pneumonia[J].Antimicrob Agents Chemother,2014,58(2):1034-1038.
[5]唐涛.白细胞介素(IL)-6及IL-8在儿童肺炎支原体肺炎中的变化和临床意义[J].当代医学,2012,18(2):73-74.
[6]姚蔚峦.肺炎支原体肺炎血清白介素-10、肿瘤坏死因子-α、D2-聚体的表达及意义[J].预防医学论坛,2017,23(1):49-51.
[7]刘淑民.肺炎支原体肺炎患儿血清白介素17和白介素23表达的意义[J].临床肺科杂志,2015,20(9):1676-1678.
[8]李玉红.Clara细胞分泌蛋白、白介素17在支原体肺炎患儿血清中表达及意义[J].临床儿科杂志,2014,32(10):948-950.
[9]张群威,任志宏.肺炎支原体肺炎患儿外周血IL-10/IL-17表达与肺功能变化的相关性[J].安徽医药,2016,20(4):762-763.
[10]赵久龄,王玉水.清肺利气通络颗粒对肺炎支原体肺炎儿童血清IL-23、IL-17水平的影响[J].云南中医中药杂志,2017,38(7):16-20.
[11]彭燕.血清IL-6、hs-CRP浓度测定在小儿肺炎支原体肺炎治疗中的临床意义[J].中国医药导报,2011,8(35):97-98.
[12]蔡惠东,郁志伟,张滨.儿童肺炎支原体肺炎血清TNF-α、IL-6、IL-8和IL-10水平测定及临床意义[J].海南医学院学报,2014,20(11):1593-1595.
[13]JIANG W,YAN Y,JI W,et al.Clinical significance of different bacterial load of Mycoplasma pneumoniae in patients with Mycoplasma pneumoniae pneumonia[J].Braz J Infect Dis,2014,18(2):124-128.
[14]WANG M,WANG Y,YAN Y,et al.Clinical and laboratory profiles of refractory Mycoplasma pneumoniae pneumonia in children[J].Infect Dis,2014,29:18-23.
[15]邓加忠,佘刚,何川.小儿肺炎支原体感染的研究进展[J].中国处方药,2015,13(6):31-32.
[16]JIANG W,QIAN L,LIANG H,et al.Relationships between the varied ciliated respiratory epithelium abnormalities and severity of Mycoplasma pneumoniae pneumonia[J].Scand J Infect Dis,2014,46(7):486-492.
[17]王冬梅,姜采荣,王茹,等.肺炎支原体肺炎患儿血清白介素-23/白介素-17的表达[J].临床儿科杂志,2013,31(10):933-936.
[18]鲍芳,曲久鑫,刘振嘉,等.儿童大环内酯耐药支原体肺炎的临床特点及治疗转归[J].中华结核和呼吸杂志,2013,36(10):756-761.
[19]谭洪珍.探究TNF-α、IL-13在支原体肺炎儿童血中的表达及临床意义[J].中国妇幼健康研究,2015,26(4):828-830.
[2]王有鹏,景伟超,谭杰军.儿童支原体肺炎IFN-γ、IL-4水平中西医研究现状[J].中医药学报,2014,42(4):133-135.
[3]KAWAI Y,MIYASHITA N,KUBO M,et al.Therapeutic efficacy of macrolides,minocycline,and tosufloxacin against macrolide-resistant Mycoplasma pneumoniae pneumonia in pediatric patients[J].Antimicrob A-gents Chemother,2013,57(5):2252-2258.
[4]ZHOU Y,ZHANG Y,SHENG Y,et al.More complications occur in macrolide-resistant than in macrolidesensitive Mycoplasma pneumoniae pneumonia[J].Antimicrob Agents Chemother,2014,58(2):1034-1038.
[5]唐涛.白细胞介素(IL)-6及IL-8在儿童肺炎支原体肺炎中的变化和临床意义[J].当代医学,2012,18(2):73-74.
[6]姚蔚峦.肺炎支原体肺炎血清白介素-10、肿瘤坏死因子-α、D2-聚体的表达及意义[J].预防医学论坛,2017,23(1):49-51.
[7]刘淑民.肺炎支原体肺炎患儿血清白介素17和白介素23表达的意义[J].临床肺科杂志,2015,20(9):1676-1678.
[8]李玉红.Clara细胞分泌蛋白、白介素17在支原体肺炎患儿血清中表达及意义[J].临床儿科杂志,2014,32(10):948-950.
[9]张群威,任志宏.肺炎支原体肺炎患儿外周血IL-10/IL-17表达与肺功能变化的相关性[J].安徽医药,2016,20(4):762-763.
[10]赵久龄,王玉水.清肺利气通络颗粒对肺炎支原体肺炎儿童血清IL-23、IL-17水平的影响[J].云南中医中药杂志,2017,38(7):16-20.
[11]彭燕.血清IL-6、hs-CRP浓度测定在小儿肺炎支原体肺炎治疗中的临床意义[J].中国医药导报,2011,8(35):97-98.
[12]蔡惠东,郁志伟,张滨.儿童肺炎支原体肺炎血清TNF-α、IL-6、IL-8和IL-10水平测定及临床意义[J].海南医学院学报,2014,20(11):1593-1595.
[13]JIANG W,YAN Y,JI W,et al.Clinical significance of different bacterial load of Mycoplasma pneumoniae in patients with Mycoplasma pneumoniae pneumonia[J].Braz J Infect Dis,2014,18(2):124-128.
[14]WANG M,WANG Y,YAN Y,et al.Clinical and laboratory profiles of refractory Mycoplasma pneumoniae pneumonia in children[J].Infect Dis,2014,29:18-23.
[15]邓加忠,佘刚,何川.小儿肺炎支原体感染的研究进展[J].中国处方药,2015,13(6):31-32.
[16]JIANG W,QIAN L,LIANG H,et al.Relationships between the varied ciliated respiratory epithelium abnormalities and severity of Mycoplasma pneumoniae pneumonia[J].Scand J Infect Dis,2014,46(7):486-492.
[17]王冬梅,姜采荣,王茹,等.肺炎支原体肺炎患儿血清白介素-23/白介素-17的表达[J].临床儿科杂志,2013,31(10):933-936.
[18]鲍芳,曲久鑫,刘振嘉,等.儿童大环内酯耐药支原体肺炎的临床特点及治疗转归[J].中华结核和呼吸杂志,2013,36(10):756-761.
[19]谭洪珍.探究TNF-α、IL-13在支原体肺炎儿童血中的表达及临床意义[J].中国妇幼健康研究,2015,26(4):828-830.