海州香薷离体再生技术研究

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英文篇名：Regeneration Technology of Elsholtzia Haichouensis Sun in vitro 作者：康薇 ; 刘远河 英文作者：KANG Wei;LIU Yuan-he;Hubei Key Laboratory of Mine Environmental Pollution Control & Remediation; 关键词：海州香薷 ; 组培育苗 ; 植物修复 ; 铜污染土壤 英文关键词：Elsholtzia haichouensis Sun;;tisse culture and seeding growth;;phytoremediation;;copper contaminated soil 中文刊名：HBHG 英文刊名：Chemistry & Bioengineering 机构：矿区环境污染控制与修复湖北省重点实验室; 出版日期：2016-06-13 10:11 出版单位：化学与生物工程 年：2016 期：v.33;No.233 基金：湖北省科技厅公益性科技研究项目(2012DCA23001);; 湖北省高等学校科技创新团队项目(T201317) 语种：中文; 页：HBHG201606013 页数：4 CN：06 ISSN：42-1710/TQ 分类号：60-62+66

摘要

为探讨适合海州香薷的组培育苗技术,解决生产实践中海州香薷种苗供应不足的问题,选择大冶铜绿山矿区生长的野生海州香薷为试材,通过外植体选择、激素配比优化、愈伤组织诱导、不定芽分化和生根培养等步骤,建立了海州香薷的离体再生体系。结果表明,以海州香薷带芽节段为外植体,愈伤组织诱导的最适培养基为MS+0.5mg·L~(-1) 6-BA+0.5mg·L~(-1) NAA+25g·L~(-1)蔗糖+6.5g·L~(-1)琼脂+3.2mg·L~(-1) CuSO_4,愈伤组织分化不定芽的最适培养基为MS+0.5 mg·L~(-1) 6-BA+0.1 mg·L~(-1) NAA+30g·L~(-1)蔗糖+7.0g·L~(-1)琼脂+3.2 mg·L~(-1)CuSO_4,在此条件下,愈伤组织诱导率最高可达98.3%,不定芽分化率最高可达100%,单个愈伤组织不定芽分化数平均达7.5个。将不定芽置于MS+0.5 mg·L~(-1) 6-BA+0.1 mg·L~(-1) NAA+30g·L~(-1)蔗糖+7.0g·L~(-1)琼脂+3.2mg·L~(-1) CuSO_4上很容易生根,生根组培苗在装有铜污染土壤的培养盆中成活率可达100%。
        In order to make up for the shortage of production practice in Elsholtzia haichouensis Sun seeding,tissue cultural technique has been studied.In the paper,we choose the experimental material of Elsholtzia haichouensis Sun which grows in Daye Tonglu Mountain,Hubei,China,and establish its regeneration systemin vitro by choosing explants,regulating the ratio of plant hormone,inducing the callus,differentiating the adventitious bud and rooting culture.The results show that using bud segments as explants,the best medium of callus induction and the bud differentiation are as follows:MS+6-BA 0.5mg·L~(-1)+NAA 0.5mg·L~(-1)+sucrose 25g·L~(-1)+agar 6.5g·L~(-1)+CuSO_43.2mg·L~(-1),MS+6-BA 0.5mg·L~(-1)+NAA 0.1mg·L~(-1)+sucrose 30g·L~(-1)+agar 7.0g·L~(-1)+CuSO_43.2mg·L~(-1),respectively.Under above conditions,callus induction rate and adventitious bud differentiation rate reach up to 98.3% and 100%,respectively.The average quantity of buds producing from the signal callus is 7.5.The medium component of MS+6-BA 0.5 mg·L~(-1)+NAA 0.1mg·L~(-1)+sucrose 30g·L~(-1)+agar 7.0g·L~(-1)+CuSO_43.2mg·L~(-1) is beneficial to buds′rooting.The survival rate of rooting buds could reach 100%in cultivating basin with the copper contaminated soils.

引文

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