上皮间质转化相关蛋白在结肠癌及同期肝转移组织中的表达差异
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摘要
目的研究结肠癌伴随肝转移患者原发灶及转移组织中E钙黏素,N黏黏素和波形蛋白的表达差异。方法选取62例结肠癌患者,分为3组:无转移的结肠癌组(n=42),伴肝转移的结肠癌原发灶组(n=20)和肝转移灶组(n=20)。用荧光定量PCR、Western blot和免疫组化法检测E钙黏素,N-钙黏素和波形蛋白的表达,及其与结肠癌临床病理特征的关系。结果伴肝转移组结肠癌较无转移结肠癌组、E-钙黏素表达下调(P<0.01),N-钙黏素和波形蛋白表达上调(P<0.05)。结肠癌原发灶与肝转移灶相比较,E-钙黏素表达平均吸光度值0.10±0.01比0.06±0.02(P<0.01);N钙黏素表达平均吸光度值0.07±0.02比0.09±0.01(P<0.05);波形蛋白表达平均吸光度值0.06±0.01比0.09±0.02(P<0.01)。E钙黏素、N钙黏素和波形蛋白的表达与TNM分期和淋巴结转移均密切相关(P<0.05)。结论 E-钙黏素,N-钙黏素和波形蛋白在结肠癌及伴随肝转移组织的表达差异对其预后可能具有重要意义。
Objective To investigate the expression of E-cadherin, N-cadherin and vimentin between primary colon cancer and synchronous liver metastasis tissue. Methods To examine the expression of E-cadherin, N-cadherin and vimentin by real-time PCR, Western blot and immunohistochemistry in colon cancer without distant metastases(n=42), primary colon cancer tissues with liver metastases(n=20) and liver metastasis tissues(n=20). Furthermore, to analyze its association with pathologic parameter. Results The expression of E-cadherin was down-regulated(P<0.01), and N-cadherin and vimentin were up-regulated(P<0.05) in primary colon cancer with liver metastasis group as compared with colon cancer without metastasis. The absorbance value of the E-cadherin, N-cadherin and vimentin(0.10±0.01 vs 0.06±0.02, 0.07±0.02 vs 0.09±0.01 and 0.06±0.01 vs 0.09±0.02) was significantly different between primary colon cancer and corresponding liver metastasis(P<0.01,P<0.05 and P<0. 01). There exists close correlation between the expression of E-cadherin,N-cadherin and vimentin and TNM stage and lymphynode( P < 0. 05). Conclusions The differential expression of E-cadherin,N-cadherin and vimentin between primary colon cancer and liver metastasis is important for its prognosis.
Objective To investigate the expression of E-cadherin, N-cadherin and vimentin between primary colon cancer and synchronous liver metastasis tissue. Methods To examine the expression of E-cadherin, N-cadherin and vimentin by real-time PCR, Western blot and immunohistochemistry in colon cancer without distant metastases(n=42), primary colon cancer tissues with liver metastases(n=20) and liver metastasis tissues(n=20). Furthermore, to analyze its association with pathologic parameter. Results The expression of E-cadherin was down-regulated(P<0.01), and N-cadherin and vimentin were up-regulated(P<0.05) in primary colon cancer with liver metastasis group as compared with colon cancer without metastasis. The absorbance value of the E-cadherin, N-cadherin and vimentin(0.10±0.01 vs 0.06±0.02, 0.07±0.02 vs 0.09±0.01 and 0.06±0.01 vs 0.09±0.02) was significantly different between primary colon cancer and corresponding liver metastasis(P<0.01,P<0.05 and P<0. 01). There exists close correlation between the expression of E-cadherin,N-cadherin and vimentin and TNM stage and lymphynode( P < 0. 05). Conclusions The differential expression of E-cadherin,N-cadherin and vimentin between primary colon cancer and liver metastasis is important for its prognosis.
引文
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[6]. Hur K, Toiyama Y, Takahashi M, et al. MicroRNA-200c modulates epithelial-to-mesenchymal transition (EMT) in human colorectal cancer metastasis[J]. Gut, 2013,62:1315-1326.
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[8] Tsai JH, Donaher JL, Murphy DA, et al. Spatiotemporal regulation of epithelial-mesenchymal transition is essential for squamous cell carcinoma metastasis[J]. Cancer Cell, 2012,22:725-736.
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