黄芩苷体外对耐甲氧西林金黄色葡萄球菌活性影响的研究
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摘要
目的研究从中药黄芩干燥根中提取分离药效成分黄芩苷体外对耐甲氧西林金黄色葡萄球菌活性的影响。方法以琼脂扩散法、液体稀释微孔板法检测黄芩苷对MRSA的抑菌环大小、最低抑菌浓度(MIC)和最低杀菌浓度(MBC);以96孔酶标板结晶紫法检测黄芩苷对MRSA的最小生物膜清除浓度(MBEC)。结果与DMSO比较,黄芩苷与氯霉素(阳性对照药物)对MRSA的抑菌环半径显著增加(P<0.01),与氯霉素比较,黄芩苷对MRSA的抑菌环半径显著缩小(P<0.05);黄芩苷对MRSA的MIC为0.5mg/ml;MBC为1mg/ml;黄芩苷对MRSA的MBEC为0.5mg/ml。结论黄芩苷对MRSA有一定的抗菌、抑菌作用,其效应机制可能与其抑制细菌生物膜的形成有关。
Objective To study the baicalin extracted and purified from dried root of Scutellaria baicalensis who can affect the the activity of Methicillin-resistant Staphylococcus aureus(MRSA) in vitro. Methods Agar diffusion test and broth dilution test were used to assay the size of antibacterial annulus, minimum inhibitory concentration(MIC) and minimum bactericidal concentration(MBC) after the MRSA being treated by baicalin; The crystal violet staining method measured on 96 well Elisa-plate was employed to analyze minimal biofilm eradication concentration(MBEC) after the MRSA being treated by baicalin. Results Compare with DMSO, antibacterial annulus radius of MRSA significantly increased when it was disposed by baicalin and chloramphenicol(positive control drug)(P<0.01). The MIC, MBC and MBEC of baicalin directed against MRSA were 0.5 mg/ml, 1 mg/ml and 0.5 mg/ml, respectively. Conclusion MRSA can be suppressed by baicalin moderately, and the effect mechanism may be related to the inhibition of bacterial biofilm formation.
Objective To study the baicalin extracted and purified from dried root of Scutellaria baicalensis who can affect the the activity of Methicillin-resistant Staphylococcus aureus(MRSA) in vitro. Methods Agar diffusion test and broth dilution test were used to assay the size of antibacterial annulus, minimum inhibitory concentration(MIC) and minimum bactericidal concentration(MBC) after the MRSA being treated by baicalin; The crystal violet staining method measured on 96 well Elisa-plate was employed to analyze minimal biofilm eradication concentration(MBEC) after the MRSA being treated by baicalin. Results Compare with DMSO, antibacterial annulus radius of MRSA significantly increased when it was disposed by baicalin and chloramphenicol(positive control drug)(P<0.01). The MIC, MBC and MBEC of baicalin directed against MRSA were 0.5 mg/ml, 1 mg/ml and 0.5 mg/ml, respectively. Conclusion MRSA can be suppressed by baicalin moderately, and the effect mechanism may be related to the inhibition of bacterial biofilm formation.
引文
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[12] 崔新洁,夏瑾,邵铁娟,等.中药抗耐甲氧西林金黄色葡萄球菌生物膜研究进展[J].中国中医药信息杂志,2017,24(2):132.
[13] 王艺竹,王宏涛,韩雪,等.连花清瘟胶囊水提物对耐甲氧西林金黄色葡萄球菌细菌生物膜的影响[J].中华医院感染学杂志,2015,25(4):727.
[2] 林云万,张旭,李晓宁,等.耐甲氧西林金黄色葡萄球菌的分子分型及耐药性分析[J].现代医院,2017,17(6):898.
[3] 傅若秋,卢来春,李卓恒,等.31种中药单体对耐甲氧西林金黄色葡萄球菌的抗菌作用研究[J].中国药业,2014,23(4):20.
[4] 于倩,林静,祖力卡尔江,等.伊犁黑蜂蜂胶对口腔主要致龋细菌生物膜作用的实验研究[J].华西口腔医学杂志,2015,33(4):344.
[5] 詹铀超,秦笙,陈富,等.黄芩苷抗呼吸道合胞病作用的研究[J].囯际检验医学杂志,2017,38(14):1907.
[6] 冯鑫,施高翔,云云,等.黄芩苷联合氟康唑通过降低cAMP抑制白念珠菌酵母-菌丝形态转化[J].中国真菌学杂志,2016,11(1):8.
[7] 李玲.黄芩苷对牙周炎大鼠牙移动保持阶段VEGF、BMP-2及IL-1β表达的影响[J].中国生化药物杂志,2015,35(3):32.
[8] 岑艳灵,李亚楠,孔晋亮,等.黄芩苷联合美罗培南对小鼠腹腔铜绿假单胞菌早期生物被膜的体内影响[J].中华医院感染学杂志,2017,27(1):39.
[9] 邓志燕,万强.黄芩苷对幽门螺杆菌诱导人胃黏膜上皮GES-1细胞损伤的保护作用及机制[J].中国实验方剂学杂志,2017,23(9):145.
[10] 赵旭,赵璐,严向明,等.细菌生物膜的形成与导管相关性尿路感染的关系[J].中华医院感染学杂志,2017,27(18):4154.
[11] 王宽,解英,汪虹,等.细菌生物膜与慢性创面愈合[J].中华医院感染学杂志,2016,26(19):4554.
[12] 崔新洁,夏瑾,邵铁娟,等.中药抗耐甲氧西林金黄色葡萄球菌生物膜研究进展[J].中国中医药信息杂志,2017,24(2):132.
[13] 王艺竹,王宏涛,韩雪,等.连花清瘟胶囊水提物对耐甲氧西林金黄色葡萄球菌细菌生物膜的影响[J].中华医院感染学杂志,2015,25(4):727.