金叶紫穗槐抗寒生理研究（英文）
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摘要
以二年生金叶紫穗槐(Amorpha fruticosa L.cv.Jinye)和紫穗槐(对照)枝条为对象,分别测定自然降温下叶片的电导率和丙二醛、可溶性糖、可溶性蛋白、脯氨酸的含量以及人工低温下的SOD、POD、CAT活性的变化,探讨金叶紫穗槐在低温条件下的适应能力。结果表明,随着自然温度的降低,金叶紫穗槐的丙二醛含量低于对照;渗透调节物质可溶性糖、可溶性蛋白和脯氨酸含量升高,比对照有更强的低温耐受能力;SOD、POD、CAT活性持续升高,高于对照,抵御低温的能力优于对照。不过金叶紫穗槐的相对电导率与对照的差异不显著。
With two-year-old Amorpha fruticosa L. cv. Jinye and A. fruticosa as the research objects, the relative conductivity, MDA content, soluble sugar content, soluble protein content and proline content in leaf were determined during natural drop in temperature, and the SOD, POD and COD activity in leaf were determined under artificial low temperature to explore the adaptability of A. fruticosa L. cv. Jinye to low temperature. The results showed that with the decrease in natural temperature,the content of MDA in A. fruticosa L. cv. Jinye was lower than that in the control,and the contents of osmotic adjustment substances(soluble sugar, soluble protein and proline) increased, indicating that A. fruticosa L. cv. Jinye had a stronger tolerance to low temperature compared with A. fruticosa. The activity of SOD, POD and CAT in A. fruticosa L. cv. Jinye increased continuously, higher than that in the control, indicating that the resisting ability of A. fruticosa L. cv. Jinye against low temperature was superior to that of the control. There was no significant difference in relative conductivity between A. fruticosa L. cv. Jinye and A. fruticosa.
With two-year-old Amorpha fruticosa L. cv. Jinye and A. fruticosa as the research objects, the relative conductivity, MDA content, soluble sugar content, soluble protein content and proline content in leaf were determined during natural drop in temperature, and the SOD, POD and COD activity in leaf were determined under artificial low temperature to explore the adaptability of A. fruticosa L. cv. Jinye to low temperature. The results showed that with the decrease in natural temperature,the content of MDA in A. fruticosa L. cv. Jinye was lower than that in the control,and the contents of osmotic adjustment substances(soluble sugar, soluble protein and proline) increased, indicating that A. fruticosa L. cv. Jinye had a stronger tolerance to low temperature compared with A. fruticosa. The activity of SOD, POD and CAT in A. fruticosa L. cv. Jinye increased continuously, higher than that in the control, indicating that the resisting ability of A. fruticosa L. cv. Jinye against low temperature was superior to that of the control. There was no significant difference in relative conductivity between A. fruticosa L. cv. Jinye and A. fruticosa.
引文
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[16]DONG L(董亮),HE YZ(何永志),WANG YL(王远亮),et al.Research progress on application of superoxide dismutase(SOD)(超氧化物歧化酶(SOD)的应用研究进展)[J].Journal of Agricultural Science and Technology(中国农业科技导报),2013,15(5):53-58.
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[19]ZHANG J(张捷),YANG SM(杨淑梅),WANG L(王玲).Cold resistance of two"Zhuliu"varieties under low temperature stress(低温胁迫下两个“竹柳”品系的抗寒性)[J].Scientia Silvae Sinicae(林业科学),2013,49(9):158-164.
[20]FANG XP(方小平),LI CY(李昌艳),HU GP(胡光平).Studies on the cold resistance of four Magnoliaceae species in Guizhou Province(贵州4种木兰科植物幼苗的抗寒性研究)[J].Forest Research(林业科学研究),2010,23(6):862-865.
[21]HUANG HT(黄海涛),YU JZ(余继忠),WANG XB(王贤波),et al.Study on physiological characters of new shoot in different cold-resistant tea varieties in autumn(不同抗寒性茶树品种秋季新梢的生理特性研究)[J].Acta Agriculturae Zhejiangensis(浙江农业学报),2014,26(4):925-928.
[22]WANG HL(王红亮),CHEN LL(陈丽丽).Effect of low temperature stress on relative conductivity of nine kinds of greening trees(低温胁迫对9种绿化树木相对电导率的影响)[J].Jiangsu Agricultural Sciences(江苏农业科学),2013,41(4):167-169.
[23]CHEN Y(陈钰),GUO AH(郭爱华),YAO YJ(姚月俊),et al.The change of POD enzymes,relative conductivities and soluble protein in flower of almond under cold stress(低温胁迫下杏花器官内POD、相对电导率和可溶性蛋白含量的变化)[J].Journal of Shanxi Agricultural Sciences(山西农业科学),2007,35(3):30-32.
[24]ZHONG Q(仲强),MENG K(康蒙),GUO M(郭明),et al.Leaf frost sensitivity and percentage of electrolyte leakage of the evergreen woody species in Tiantong region,Zhejiang Province(浙江天童常绿木本植物的叶片相对电导率及抗寒性)[J].Journal of East China Normal University(Natural Science)(华东师范大学学报(自然科学版)),2011(4):45-52.
[2]Shanghai Institute of Plant Physiology,Chinese Academy of Sciences(中国科学院上海植物生理研究所).Modern plant physiology experiment guide(现代植物生理学实验指南)[M].Beijing:Science Press(北京:科学出版社),2004.
[3]WANG XK(王学奎).Principles and technology of physiological and biochemical experiments of plants(植物生理生化实验原理和技术)[M].Beijing:Higher Education Press(北京:高等教育出版社),2015.
[4]CHEN LL(陈丽丽),LIN F(蔺芳),ZHANG JY(张家洋).Influence of temperature shock on the physiological indexes of cold resistance of 5 evergreen plants from northern China(温度骤变对5种北方常绿植物抗寒性生理指标的影响)[J].Journal of West China Forestry Science(西部林业科学),2016,45(2):94-98.
[5]WANG N(王宁),YUAN ML(袁美丽),DING B(丁博).The effect of cold stress on the cold resistance physiology of two camphor species(低温胁迫对两种樟树抗寒性生理的影响)[J].Journal of Shandong Agricultural University(Natural Science Edition)(山东农业大学学报(自然科学版)),2016,47(1):37-42.
[6]ZENG W(曾雯),JIN XL(金晓玲),XING W(邢文),et al.Comparison of cold resistance among 9 evergreen hybrid ilex cultivars(9个常绿杂交冬青的抗寒能力比较)[J].Acta Phytophysiologica Sinica(植物生理学报),2016,52(1):55-61.
[7]GAO JC(高京草),WANG HX(王慧霞),LI XX(李西选).Relationship between soluble protein,MDA and jujube tree cold hardiness(可溶性蛋白、丙二醛含量与枣树枝条抗寒性的关系研究)[J].Northern Horticulture(北方园艺),2010(23):18-20.
[8]LIN Y(林艳),GUO WZ(郭伟珍),XU ZH(徐振华),et al.Cold resistance and changes on MDA and soluble sugar of leaves of Ligustrum lucidum Ait in winter(大叶女贞抗寒性及冬季叶片丙二醛和可溶性糖含量的变化)[J].Chinese Agricultural Science Bulletin(中国农学通报),2012,28(25):68-72.
[9]SHI JC(石进朝),LI GL(李桂伶),CHEN XX(陈秀新),et al.Physiological mechanism of Lonicera maachii"Canglvqi"to cold resistance(长绿期金银木耐寒生理机制研究)[J].Acta Botanica BorealiOccidentalia Sinica(西北植物学报),2009,29(1):111-115.
[10]WEI XQ(魏秀清),XU L(许玲),ZHANG XJ(章希娟),et al.Physiological responses of wax apple to low temperatures and its cold resistance(莲雾对低温胁迫的生理响应及抗寒性分析)[J].Journal of Fruit Science(果树学报),2016,33(1):73-80.
[11]YANG YZ(杨玉珍),CHEN G(陈刚),PENG FR(彭方仁),et al.Differences in water and osmoregulation substance contents in Toona sinensis from different provenances under low temperature stress and their correlation to cold tolerance(低温胁迫下不同种源香椿含水量和渗透调节物质含量差异及其与抗寒性的相关性)[J].Journal of Plant Resources and Environment(植物资源与环境学报),2014,23(4):47-54.
[12]LIU DL(刘杜玲),ZHANG BY(张博勇),SUN HM(孙红梅),et al.Comprehensive evaluation on cold resistance of early fruiting walnut cultivars(早实核桃不同品种抗寒性综合评价)[J].Acta Horticulturae Sinica(园艺学报),2015,42(3):545-553.
[13]SHEN XH(申晓慧),JIANG C(姜成),FENG P(冯鹏),et al.Research on changing of several cold resistance physiological indexes of 6 kinds of alfalfa before and after overwintering(6种紫花苜蓿越冬前后几个抗寒生理指标变化研究)[J].Journal of Agriculture(农学学报)0,2015,5(12):94-98.
[14]LI YY(李叶云),SHU XT(舒锡婷),ZHOU YQ(周月琴),et al.Change in physiological characteristics and cold resistances evaluation of three cultivars of Camellia sinensis during natural overwintering period(自然越冬过程中3个茶树品种的生理特性变化及抗寒性评价)[J].Journal of Plant Resources and Environment(植物资源与环境学报),2014,23(3):52-58.
[15]PEI W(裴文),LI P(李鹏),PEI HC(裴海潮),et al.Comparative analysis of physiological index related to cold resistance of nine Magnoliaceae plants under natural and artificial low temperature conditions(低温条件下9种木兰科植物抗寒性研究)[J].Journal of Henan Agricultural Sciences(河南农业科学),2014,43(4):101-105.
[16]DONG L(董亮),HE YZ(何永志),WANG YL(王远亮),et al.Research progress on application of superoxide dismutase(SOD)(超氧化物歧化酶(SOD)的应用研究进展)[J].Journal of Agricultural Science and Technology(中国农业科技导报),2013,15(5):53-58.
[17]LIANG YR(梁艳荣),HU XH(胡晓红),ZHANG YL(张颖力),et al.Progress on physiological function research of plant peroxidase(植物过氧化物酶生理功能研究进展)[J].Journal of Inner Mongolia Agricultural University(内蒙古农业大学学报),2003,24(2):110-113.
[18]CHEN X(陈荀),WANG CL(王春雷),PENG HM(彭宏梅),et al.Research on cold resistance of Syringa pekinensis"Beijing Gold"under temperature stress(低温胁迫下黄花丁香的抗寒性研究)[J].Journal of Northeast Normal University(Natural Science Edition)(东北师大学报(自然科学版)),2014,46(4):122-129.
[19]ZHANG J(张捷),YANG SM(杨淑梅),WANG L(王玲).Cold resistance of two"Zhuliu"varieties under low temperature stress(低温胁迫下两个“竹柳”品系的抗寒性)[J].Scientia Silvae Sinicae(林业科学),2013,49(9):158-164.
[20]FANG XP(方小平),LI CY(李昌艳),HU GP(胡光平).Studies on the cold resistance of four Magnoliaceae species in Guizhou Province(贵州4种木兰科植物幼苗的抗寒性研究)[J].Forest Research(林业科学研究),2010,23(6):862-865.
[21]HUANG HT(黄海涛),YU JZ(余继忠),WANG XB(王贤波),et al.Study on physiological characters of new shoot in different cold-resistant tea varieties in autumn(不同抗寒性茶树品种秋季新梢的生理特性研究)[J].Acta Agriculturae Zhejiangensis(浙江农业学报),2014,26(4):925-928.
[22]WANG HL(王红亮),CHEN LL(陈丽丽).Effect of low temperature stress on relative conductivity of nine kinds of greening trees(低温胁迫对9种绿化树木相对电导率的影响)[J].Jiangsu Agricultural Sciences(江苏农业科学),2013,41(4):167-169.
[23]CHEN Y(陈钰),GUO AH(郭爱华),YAO YJ(姚月俊),et al.The change of POD enzymes,relative conductivities and soluble protein in flower of almond under cold stress(低温胁迫下杏花器官内POD、相对电导率和可溶性蛋白含量的变化)[J].Journal of Shanxi Agricultural Sciences(山西农业科学),2007,35(3):30-32.
[24]ZHONG Q(仲强),MENG K(康蒙),GUO M(郭明),et al.Leaf frost sensitivity and percentage of electrolyte leakage of the evergreen woody species in Tiantong region,Zhejiang Province(浙江天童常绿木本植物的叶片相对电导率及抗寒性)[J].Journal of East China Normal University(Natural Science)(华东师范大学学报(自然科学版)),2011(4):45-52.