一株产吲哚乙酸菌的筛选、鉴定与优化
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摘要
为筛选出一株产吲哚乙酸(IAA)能力较好的菌,对采自辽宁省大连市郊区农田的植物根际土壤中的植物根际促生菌进行分离筛选,得到16株有产IAA活性的菌。从中选出产IAA量最高的菌株对其进行鉴定,并通过单因素试验与响应面法优化该菌株产IAA的培养条件。结果表明,该菌为芽孢杆菌属,最适生长条件为培养时间45 h,以0.29%的牛肉膏为碳源,1.00%酵母浸粉为氮源,添加金属离子为0.78%硫酸镁。优化后菌株IAA产量由未优化前的83.65 mg/L提高至106.11 mg/L。
To screen a strain with good indole-3-acetic acid(IAA) producing ability, 16 strains of IAA-producing bacteria were isolated from the rhizosphere soil of plants collected from suburban farmland in Dalian of Liaoning Province. The strain with the highest IAA production was selected and identified. The cultivation conditions of IAA production were optimized by single factor test and response surface methodology. The results showed that the strain was Bacillus sp. The optimum growth conditions were as follows: culture time of 45 h, 0.29% beef extract as carbon source, 1.00% yeast extract as nitrogen source and 0.78% Mg~(2+) as metal ions. The IAA yield increased from 83.65 to 106.11 mg/L after optimization.
To screen a strain with good indole-3-acetic acid(IAA) producing ability, 16 strains of IAA-producing bacteria were isolated from the rhizosphere soil of plants collected from suburban farmland in Dalian of Liaoning Province. The strain with the highest IAA production was selected and identified. The cultivation conditions of IAA production were optimized by single factor test and response surface methodology. The results showed that the strain was Bacillus sp. The optimum growth conditions were as follows: culture time of 45 h, 0.29% beef extract as carbon source, 1.00% yeast extract as nitrogen source and 0.78% Mg~(2+) as metal ions. The IAA yield increased from 83.65 to 106.11 mg/L after optimization.
引文
[1] 甄雪冰.土传病害生防菌株的筛选鉴定及防治效果研究[D].南京:南京林业大学,2009.
[2] ANELISE B,ADRIANA A.Plant growth promoting rhizobacteria (PGPR):their potential as antagonists and biocontrol agents[J].Genetics and Molecular Biology,2012,35(4):1044-1051.
[3] 罗静静.连作棉田PGPR菌株的定殖性能及抗菌活性物质研究[D].乌鲁木齐:新疆农业大学,2015.
[4] 吴翔,甘炳成,谢丽源,等.响应面法优化细菌MY07产IAA液体发酵培养基[J].生物技术,2015,25(2):201-204.
[5] DUBEIKOVSKY A N,MORDUKHOVA E A,KOCHETKOV V V,et al.Growth promoting of blackcurrant softwood cuttings by recombinant strain Pesudomonas fluorescens B-SP53a synthesizing an increased amount of indole-3-acetic acid[J].Soil Biology and Biochemistry,1993,25(9):1277-1281.
[6] 赵柏霞,刘浩强,孙丽娜,等.甜樱桃根际IAA产生菌的筛选、鉴定及最佳产素条件优化[J].中国南方果树,2017,46(3):23-28.
[7] 高君君.植物根际促生菌的筛选及其对番茄青枯病的生防作用[D].扬州:扬州大学,2011.
[8] 吴翔,甘炳成,黄忠乾,等.一株产IAA菌株的筛选、鉴定及培养条件优化[J].四川农业大学学报,2014,32(4):432-435.
[9] MONIS P T,STEVEN G,SAINT C P,et al.Comparis on of SYTO9 and SYBR Green Ⅰ for realtime polymerase chain reaction and investigation of the effect of dye concentration on amplification and DNA melting curve analysis[J].Analytical Biochemistry,2005,340(1):24-34.
[10] VOLKNMR K M,BREMER E.Effects of seed inoculation with a strain of Pseudomonas fluorescens on root growth and activity of wheat in well-watered and drought-stressed glass-fronted rhizotrons[J].Canadian Journal of Plant Sciences,1998,78:545-551.
[2] ANELISE B,ADRIANA A.Plant growth promoting rhizobacteria (PGPR):their potential as antagonists and biocontrol agents[J].Genetics and Molecular Biology,2012,35(4):1044-1051.
[3] 罗静静.连作棉田PGPR菌株的定殖性能及抗菌活性物质研究[D].乌鲁木齐:新疆农业大学,2015.
[4] 吴翔,甘炳成,谢丽源,等.响应面法优化细菌MY07产IAA液体发酵培养基[J].生物技术,2015,25(2):201-204.
[5] DUBEIKOVSKY A N,MORDUKHOVA E A,KOCHETKOV V V,et al.Growth promoting of blackcurrant softwood cuttings by recombinant strain Pesudomonas fluorescens B-SP53a synthesizing an increased amount of indole-3-acetic acid[J].Soil Biology and Biochemistry,1993,25(9):1277-1281.
[6] 赵柏霞,刘浩强,孙丽娜,等.甜樱桃根际IAA产生菌的筛选、鉴定及最佳产素条件优化[J].中国南方果树,2017,46(3):23-28.
[7] 高君君.植物根际促生菌的筛选及其对番茄青枯病的生防作用[D].扬州:扬州大学,2011.
[8] 吴翔,甘炳成,黄忠乾,等.一株产IAA菌株的筛选、鉴定及培养条件优化[J].四川农业大学学报,2014,32(4):432-435.
[9] MONIS P T,STEVEN G,SAINT C P,et al.Comparis on of SYTO9 and SYBR Green Ⅰ for realtime polymerase chain reaction and investigation of the effect of dye concentration on amplification and DNA melting curve analysis[J].Analytical Biochemistry,2005,340(1):24-34.
[10] VOLKNMR K M,BREMER E.Effects of seed inoculation with a strain of Pseudomonas fluorescens on root growth and activity of wheat in well-watered and drought-stressed glass-fronted rhizotrons[J].Canadian Journal of Plant Sciences,1998,78:545-551.