LDH影像分级与DKK-1的负相关可能与Wnt信号通路的调控有关
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摘要
本研究选择了100例腰椎间盘突出症(lumbar disk herniation, LDH)患者为观察组,以同期健康体检者20例为对照组,采用Western blotting法检测血清中Dickkopf相关蛋白1 (dickkopf-related protein 1, DKK-1)的表达水平,用ELISA法检测两组血清中DKK-1含量,试图分析血清中DKK-1与LDH影像学分级的相关性及其作用机理。研究结果显示,LDH患者的血清中DKK-1含量水平明显低于正常对照组。Western blotting法检测发现LDH患者影像分级与DKK-1的蛋白水平呈负相关;而ELISA法检测也证实LDH患者影像分级与DKK-1水平呈负相关。LDH患者影像分级与Wnt信号通路相关蛋白β-catenin呈正相关。本研究初步说明,腰椎间盘突出症影像分级与DKK-1水平呈负相关,而该相关性与Wnt信号通路的相关蛋白β-catenin调控有关。
In this study, 100 patients with lumbar disk herniation(LDH) were selected as the observation group,and 20 healthy persons during the same period were taken as the control group. The expression level of dickkopf-related protein 1(DDK-1) in serum was detected by Western blotting method, and the content of DKK-1in serum of the two groups was detected by ELISA. We attempted to analyze the correlation between DKK-1 in serum and LDH image grading and its mechanism of action. The results showed that the serum DKK-1 level of LDH patients was significantly lower than that of the normal control group. Western blotting analysis showed that the image grade of LDH patients was negatively correlated with the protein level of DKK-1. The ELISA test also confirmed that the image grading of LDH patients was negatively correlated with the DKK-1 level, while the image grading of LDH patients was positively correlated with the Wnt signaling pathways-related proteinβ-catenin. Our preliminary study showed that the image grading of lumbar disc herniation might be negatively correlated with the DKK-1 level, which might be related to the the regulation of the protein β-catenin associated with Wnt signaling pathway.
In this study, 100 patients with lumbar disk herniation(LDH) were selected as the observation group,and 20 healthy persons during the same period were taken as the control group. The expression level of dickkopf-related protein 1(DDK-1) in serum was detected by Western blotting method, and the content of DKK-1in serum of the two groups was detected by ELISA. We attempted to analyze the correlation between DKK-1 in serum and LDH image grading and its mechanism of action. The results showed that the serum DKK-1 level of LDH patients was significantly lower than that of the normal control group. Western blotting analysis showed that the image grade of LDH patients was negatively correlated with the protein level of DKK-1. The ELISA test also confirmed that the image grading of LDH patients was negatively correlated with the DKK-1 level, while the image grading of LDH patients was positively correlated with the Wnt signaling pathways-related proteinβ-catenin. Our preliminary study showed that the image grading of lumbar disc herniation might be negatively correlated with the DKK-1 level, which might be related to the the regulation of the protein β-catenin associated with Wnt signaling pathway.
引文
Bafico A.,Liu G.,Yaniv A.,Gazit A.,and Aaronson S.A.,2001,Novel mechanism of Wnt signalling inhibition mediated by Dickkopf-1 interaction with LRP6/Arrow,Nat.Cell Biol.,3(7):683-686
Diarra D.,Stolina M.,Polzer K.,Zwerina J.,Ominsky M.S.,Dwyer D.,Korb A.,Smolen J.,Hoffmann M.,Scheinecker C.,van der Heide D.,Landewe R.,Lacey D.,Richards W.G.,and Schett G.,2007,Dickkopf-1 is a master regulator of joint remodeling,Nature Medicine,13(2):156-163
Dullerud R.,and Nakstad P.H.,1994,Ct changes after conservative treatment for lumbar disk herniation,Acta Radiologica,35(5):415-419
Funck-Brentano T.,Bouaziz W.,Marty C.,Geoffroy V.,Hay E.,and Cohen-Solal M.,2014,Dkk-1-mediated inhibition of Wnt signaling in bone ameliorates osteoarthritis in mice,Arthritis Rheumatol.,66(11):3028-3039
Glinka A.,Wu W.,Delius H.,Monaghan A.P.,Blumenstock C.,and Niehrs C.,1998,Dickkopf-1 is a member of a new family of secreted proteins and functions in head induction,Nature,391(6665):357-362
Li M.,Wu X.H.,Yin G.,and Xie Q.B.,2015,Correlation of RANKL/OPG,dickkopf-1 and bone marrow edema in rheumatoid arthritis with the complaint of knee pain,Sichuan Daxue Xuebao Yixueban,46(2):276-279
Oh H.,Chun C.H.,and Chun J.S.,2012,DKK-1 expression in chondrocytes inhibits experimental osteoarthritic cartilage destruction in mice,Arthritis.Rheum.,64(8):2568-2578
Rachner T.D.,Gobel A.,Benadmehner P.,Hofbauer L.C.,and Rauner M.,2014,Dickkopf-1 as a mediator and novel target in malignant bone disease,Cancer Lett.,346(2):172-177
Zhou F.,Meng S.,Song H.,and Claret F.X.,2013,Dickkopf-1 is a key regulator of myeloma bone disease:opportunities and challenges for therapeutic intervention,Blood Rev.,27(6):261-267
Diarra D.,Stolina M.,Polzer K.,Zwerina J.,Ominsky M.S.,Dwyer D.,Korb A.,Smolen J.,Hoffmann M.,Scheinecker C.,van der Heide D.,Landewe R.,Lacey D.,Richards W.G.,and Schett G.,2007,Dickkopf-1 is a master regulator of joint remodeling,Nature Medicine,13(2):156-163
Dullerud R.,and Nakstad P.H.,1994,Ct changes after conservative treatment for lumbar disk herniation,Acta Radiologica,35(5):415-419
Funck-Brentano T.,Bouaziz W.,Marty C.,Geoffroy V.,Hay E.,and Cohen-Solal M.,2014,Dkk-1-mediated inhibition of Wnt signaling in bone ameliorates osteoarthritis in mice,Arthritis Rheumatol.,66(11):3028-3039
Glinka A.,Wu W.,Delius H.,Monaghan A.P.,Blumenstock C.,and Niehrs C.,1998,Dickkopf-1 is a member of a new family of secreted proteins and functions in head induction,Nature,391(6665):357-362
Li M.,Wu X.H.,Yin G.,and Xie Q.B.,2015,Correlation of RANKL/OPG,dickkopf-1 and bone marrow edema in rheumatoid arthritis with the complaint of knee pain,Sichuan Daxue Xuebao Yixueban,46(2):276-279
Oh H.,Chun C.H.,and Chun J.S.,2012,DKK-1 expression in chondrocytes inhibits experimental osteoarthritic cartilage destruction in mice,Arthritis.Rheum.,64(8):2568-2578
Rachner T.D.,Gobel A.,Benadmehner P.,Hofbauer L.C.,and Rauner M.,2014,Dickkopf-1 as a mediator and novel target in malignant bone disease,Cancer Lett.,346(2):172-177
Zhou F.,Meng S.,Song H.,and Claret F.X.,2013,Dickkopf-1 is a key regulator of myeloma bone disease:opportunities and challenges for therapeutic intervention,Blood Rev.,27(6):261-267