硒化魔芋葡甘寡糖的合成及其抗氧化活性
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摘要
为获得高硒含量的葡甘寡糖,以天然魔芋葡甘聚糖经酶解制备的魔芋葡甘寡糖为原料,合成硒化魔芋葡甘寡糖,采用傅里叶变换红外光谱和核磁共振波谱对其结构进行表征,采用电感耦合等离子体质谱法检测其硒含量;利用体外抗氧化实验研究其抗氧化活性。结果表明:合成的硒化魔芋葡甘寡糖为葡甘寡糖亚硒酸酯,硒含量达5.9 g/kg,远高于富硒土壤种植魔芋提取的天然多糖;硒化魔芋葡甘寡糖对羟自由基具有很好的清除作用,可作为理想的抗氧化有机硒食品原料。
In order to obtain selenium-enriched oligo-glucomannan,selenium-containing konjac oligo-glucomannan was synthesized from konjac oligo-glucomannan,which was prepared by enzymatic hydrolysis of natural konjac glucomannan.The structure of the synthetic product was determined using infrared spectroscopy and nuclear magnetic resonance spectroscopy.The selenium content was measured using inductively coupled plasma mass spectrometry.Antioxidant activity was characterized in vitro.The results showed that selenium-containing konjac oligo-glucomannan was successfully synthesized,and the selenium content was 5.9 g/kg,which was much higher than that of natural konjac oligo-glucomannan.The product had high scavenging capacity against hydroxyl radicals,and could be used as an antioxidant ingredient in functional foods.
In order to obtain selenium-enriched oligo-glucomannan,selenium-containing konjac oligo-glucomannan was synthesized from konjac oligo-glucomannan,which was prepared by enzymatic hydrolysis of natural konjac glucomannan.The structure of the synthetic product was determined using infrared spectroscopy and nuclear magnetic resonance spectroscopy.The selenium content was measured using inductively coupled plasma mass spectrometry.Antioxidant activity was characterized in vitro.The results showed that selenium-containing konjac oligo-glucomannan was successfully synthesized,and the selenium content was 5.9 g/kg,which was much higher than that of natural konjac oligo-glucomannan.The product had high scavenging capacity against hydroxyl radicals,and could be used as an antioxidant ingredient in functional foods.
引文
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[19]尹恒,李悝悝,谭海东,等.一种内切β-1,4-甘露聚糖酶编码基因及其制备与应用:201611133461.1[P].2016-12-10[2018-05-04].
[20]XIAO D,YU S,XIAO J.Antioxidant activities of alkali-soluble polysaccharides from medicinal mushroom Cordyceps taii and its chemical characteristics[J].Biomedical Research,2016,27(1):199-206.
[21]GAO J,ZHANG T,JIN Z,et al.Structural characterisation,physicochemical properties and antioxidant activity of polysaccharide from Lilium lancifolium Thunb[J].Food Chemistry,2015,169:430-438.DOI:10.1016/j.foodchem.2014.08.016.
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[24]RAVENSCROFT N,CESCUTTI P,GAVINI M,et al.Structural analysis of the O-acetylated O-polysaccharide isolated from Salmonella paratyphi A and used for vaccine preparation[J].Carbohydrate Research,2015,404:108-116.DOI:10.1016/j.carres.2014.12.002.
[25]宋玉龙,丘富安,吴秀钦,等.硒化修饰太子参多糖对免疫损伤小鼠的免疫保护作用[J].中国兽医学报,2017,37(11):2163-2167;2180.DOI:10.16303/j.cnki.1005-4545.2017.11.23.
[26]SAKR S,MAHRAN H,NOFAL A.Effect of selenium on carbimazole-induced testicular damage and oxidative stress in albino rats[J].Journal of Trace Elements in Medicine and Biology,2011,25(1):59-66.DOI:10.1016/j.jtemb.2010.07.002.
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[28]李华为,赵素云,刘晓波,等.硒多糖的抗氧化活性及与过氧化氢酶协同作用的研究[J].分析化学研究报告,2010,38(9):1256-1260.DOI:10.3724/SP.J.1096.2010.01256.
[29]王晓梅,顾涛,张忠山.硒化坛紫菜多糖的制备、结构表征和活性研究[J].农业机械,2012(12):113-116.DOI:10.16167/j.cnki.1000-9868.2012.12.040.
[30]宋逍,赵鹏,申婉容,等.款冬花硒多糖的制备及抗氧化性研究[J].食品工业科技,2013,34(13):227-231.DOI:10.13386/j.issn1002-0306.2013.13.023.
[31]商龙臣,吴少魏,张驰,等.南瓜硒多糖的制备表征及活性分析[J].食品科学,2016,37(19):48-53.DOI:10.7506/spkx1002-6630-201619008.
[32]尚德静,崔乔,高林.灵芝硒多糖SeGLP-1抑制小鼠肝腹水癌作用的研究[J].食用菌学报,2001(3):34-38.DOI:10.3969/j.issn.1005-9873.2001.03.007.
[33]SHENG Yu,LIU Guangchen,WANG Manli,et al.A selenium polysaccharide from Platycodon grandiflorum rescues PC12 cell death caused by H2O2 via inhibiting oxidative stress[J].International Journal of Biological Macromolecules,2017,104:393-399.DOI:10.1016/j.ijbiomac.2017.06.052.
[34]LIU Min,YAO Wangjinsong,ZHU Yongfa,et al.Characterization,antioxidant and antiinflammation of mycelia selenium polysaccharides from Hypsizygus marmoreus SK-03[J].Carbohydrate Polymers,2018,201:566-574.DOI:10.1016/j.carbpol.2018.08.099.
[35]YUAN Bo,YANG Xuqin,KOU Meng,et al.Selenylation of polysaccharide from the sweet potato and evaluation of antioxidant,antitumor,and antidiabetic activities[J].Journal of Agricultural and Food Chemistry,2017,65:605-617.DOI:10.1021/acs.jafc.6b04788.
[36]LIAN Kexun,ZHU Xiaoqing,CHEN Jie,et al.Selenylation modification:enhancement of the antioxidant activity of a Glycyrrhiza uralensis polysaccharide[J].Glycoconjugate Journal,2018,35(2):243-253.DOI:10.1007/s10719-018-9817-8.
[2]龙德清,刘传银,朱圣平.魔芋的开发利用与研究进展[J].食品技术,2003(11):18-20.DOI:10.13684/j.cnki.spkj.2003.11.008.
[3]李松林,崔熙,谢学键,等.魔芋属7种植物中魔芋葡甘露聚糖的检测[J].中国中药杂志,1996,21(8):456-458.DOI:10.3321/j.issn:1001-5302.1996.08.003.
[4]谢建华,谢丙清,庞杰,等.魔芋葡甘聚糖与乳清蛋白的相互作用[J].食品科学,2017,38(5):98-103.DOI:10.7506/spkx1002-6630-201705016.
[5]MAEDA M,SHIMAHARA H,SUGIYAMA N.Detailed examination of the branched structure of konjac glucomannan[J].Agricultural and Biological Chemistry,1980,44(2):245-252.DOI:10.1080/00021369.1980.10863939.
[6]WILLIAMS M A K,FOSTER T J,MARTIN D R,et al.A molecular description of the gelation mechanism of konjac mannan[J].Biomacromolecules,2000,1(3):440-450.DOI:10.1021/bm005525y.
[7]KATSURAYA K,OKUYAMA K,HATANAKA K,et al.Constitution of konjac glucomannan:chemical analysis and 13C NMRspectroscopy[J].Carbohydrate Polymers,2003,53(2):183-189.DOI:10.1016/S0144-8617(03)00039-0.
[8]SUWANNAPORN P,THEPWRONG K,TESTER R,et al.Tolerance and nutritional therapy of dietary fibre from konjac glucomannan hydrolysates for patients with inflammatory bowel disease(IBD)[J].Bioactive Carbohydrates and Dietary Fibre,2013,2(2):93-98.DOI:10.1016/j.bcdf.2013.09.005.
[9]ZHENG Q,WU Y,XU H.Effect of dietary oxidized konjac glucomannan on Schizothorax prenanti growth performance,body composition,intestinal morphology and intestinal microflora[J].Fish Physiology and Biochemistry,2015,41:733-743.DOI:10.1007/s10695-015-0042-0.
[10]BIMINGER M,PILAWA S,FLOHE L.Trends in selenium biochemistry[J].Natural Product Reports,2002,19(6):693-718.DOI:10.1039/B205802M.
[11]王磊,杜菲,孙卉,等.人体硒代谢与硒营养研究进展[J].生物技术进展,2015,5(4):285-290.DOI:10.3969/j.issn.2095-2341.2015.04.06.
[12]IPC,DONG Y,GANTHER H.New conceptsinseleniu chemoprevention[J].Cancer and Metastasis Reviews,2002(21):281-289.DOI:10.1023/A:102126302.
[13]XU X M,CARLSON B A,GRIMM T A,et al.Rhesus monkey simian immunodeficiency virus infection as a model for assessing the role of selenium in AIDS[J].Journal of Acquired Immune Deficiency Syndromes,2002,31(5):453-463.DOI:10.1097/00126334-200212150-00001.
[14]TAN J,ZHU W,WANG W,et al.Selenium in soil and endemic diseases in China[J].Science of the Total Environment,2002,284(1):227-235.DOI:10.1016/S0048-9697(01)00889-0.
[15]彭祚全,张欣,牟敏,等.富硒食品含硒量范围标准的研究[J].微量元素与健康研究,2013,30(1):41-43.
[16]卿艳,张立实.硒毒性研究进展[J].预防医学情报杂志,2012,28(3):216-218.
[17]黄峙,郑文杰,郭宝江.含硒生物大分子化合物研究进展[J].海南大学学报(自然科学版),2001(2):169-175.DOI:10.15886/j.cnki.hdxbzkb.2001.02.016.
[18]杨芳,谢娟平,王中兴,等.富硒魔芋精粉葡甘聚糖和硒含量的测定分析[J].陕西农业科学,2011,57(2):61-63.DOI:10.3969/j.issn.0488-5368.2011.02.025.
[19]尹恒,李悝悝,谭海东,等.一种内切β-1,4-甘露聚糖酶编码基因及其制备与应用:201611133461.1[P].2016-12-10[2018-05-04].
[20]XIAO D,YU S,XIAO J.Antioxidant activities of alkali-soluble polysaccharides from medicinal mushroom Cordyceps taii and its chemical characteristics[J].Biomedical Research,2016,27(1):199-206.
[21]GAO J,ZHANG T,JIN Z,et al.Structural characterisation,physicochemical properties and antioxidant activity of polysaccharide from Lilium lancifolium Thunb[J].Food Chemistry,2015,169:430-438.DOI:10.1016/j.foodchem.2014.08.016.
[22]SHENG J,SUN Y.Antioxidant properties of different molecular weight polysaccharides from Athyrium multidentatum(Doll.)Ching[J].Carbohydrate Polymers,2014,108:41-45.DOI:10.1016/j.carbpol.2014.03.011.
[23]MAJDOUB H,MANSOUR M B,CHAUBET F,et al.Anticoagulant activity of a sulfated polysaccharide from the green alga Arthrospira platensis[J].Biochimica et Biophysica Acta,2009,1790(10):1377-1381.DOI:10.1016/j.bbagen.2009.07.013.
[24]RAVENSCROFT N,CESCUTTI P,GAVINI M,et al.Structural analysis of the O-acetylated O-polysaccharide isolated from Salmonella paratyphi A and used for vaccine preparation[J].Carbohydrate Research,2015,404:108-116.DOI:10.1016/j.carres.2014.12.002.
[25]宋玉龙,丘富安,吴秀钦,等.硒化修饰太子参多糖对免疫损伤小鼠的免疫保护作用[J].中国兽医学报,2017,37(11):2163-2167;2180.DOI:10.16303/j.cnki.1005-4545.2017.11.23.
[26]SAKR S,MAHRAN H,NOFAL A.Effect of selenium on carbimazole-induced testicular damage and oxidative stress in albino rats[J].Journal of Trace Elements in Medicine and Biology,2011,25(1):59-66.DOI:10.1016/j.jtemb.2010.07.002.
[27]高玉杰,吕海涛.浒苔硒多糖的制备及其活性研究[J].化学与生物工程,2012,29(5):40-45.DOI:10.3969/j.issn.1672-5425.2012.05.012.
[28]李华为,赵素云,刘晓波,等.硒多糖的抗氧化活性及与过氧化氢酶协同作用的研究[J].分析化学研究报告,2010,38(9):1256-1260.DOI:10.3724/SP.J.1096.2010.01256.
[29]王晓梅,顾涛,张忠山.硒化坛紫菜多糖的制备、结构表征和活性研究[J].农业机械,2012(12):113-116.DOI:10.16167/j.cnki.1000-9868.2012.12.040.
[30]宋逍,赵鹏,申婉容,等.款冬花硒多糖的制备及抗氧化性研究[J].食品工业科技,2013,34(13):227-231.DOI:10.13386/j.issn1002-0306.2013.13.023.
[31]商龙臣,吴少魏,张驰,等.南瓜硒多糖的制备表征及活性分析[J].食品科学,2016,37(19):48-53.DOI:10.7506/spkx1002-6630-201619008.
[32]尚德静,崔乔,高林.灵芝硒多糖SeGLP-1抑制小鼠肝腹水癌作用的研究[J].食用菌学报,2001(3):34-38.DOI:10.3969/j.issn.1005-9873.2001.03.007.
[33]SHENG Yu,LIU Guangchen,WANG Manli,et al.A selenium polysaccharide from Platycodon grandiflorum rescues PC12 cell death caused by H2O2 via inhibiting oxidative stress[J].International Journal of Biological Macromolecules,2017,104:393-399.DOI:10.1016/j.ijbiomac.2017.06.052.
[34]LIU Min,YAO Wangjinsong,ZHU Yongfa,et al.Characterization,antioxidant and antiinflammation of mycelia selenium polysaccharides from Hypsizygus marmoreus SK-03[J].Carbohydrate Polymers,2018,201:566-574.DOI:10.1016/j.carbpol.2018.08.099.
[35]YUAN Bo,YANG Xuqin,KOU Meng,et al.Selenylation of polysaccharide from the sweet potato and evaluation of antioxidant,antitumor,and antidiabetic activities[J].Journal of Agricultural and Food Chemistry,2017,65:605-617.DOI:10.1021/acs.jafc.6b04788.
[36]LIAN Kexun,ZHU Xiaoqing,CHEN Jie,et al.Selenylation modification:enhancement of the antioxidant activity of a Glycyrrhiza uralensis polysaccharide[J].Glycoconjugate Journal,2018,35(2):243-253.DOI:10.1007/s10719-018-9817-8.