青藏高原狭果茶藨子果实提取物成分、抑菌活性及其抗疲劳活性
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摘要
以产自青藏高原的狭果茶藨子果实为原料,分别制备有机酸提取物、乙酸乙酯萃取物、正丁醇萃取物,对其进行抑菌活性和抗疲劳活性研究,以期为狭果茶藨子资源的开发利用提供理论依据。抑菌试验结果表明:狭果茶藨子果实中不同粗提物均具有一定的抑菌作用,当浓度为100 mg/mL时,乙酸乙酯萃取物Ⅱ对白色念珠菌抑制效果最好,抑菌圈直径达20 mm,表现为极敏,粗提物中总有机酸和总黄酮含量分别为(2.30±0.1)、(2.28±0.02) mg/g,抑菌圈试验效果比阳性对照药氨苄西林(10 IU/片)好,对白色念珠菌的MIC、MBC值分别为1.56、3.13 mg/mL,且其抑菌效果随提取物浓度的升高而增强。小鼠负重游泳试验表明:与空白对照组相比,狭果茶藨子果实中乙酸乙酯萃取物Ⅱ各剂量组均能显著延长小鼠负重游泳时间(p<0.05),提高小鼠肌糖原、肝糖原储备能力,增强乳酸脱氢酶活性,同时降低血清尿素氮含量,效果优于红景天阳性对照组,说明狭果茶藨子果实具有良好的抗疲劳活性。
In order to provide a theoretical basis for the exploitation and utilization of Ribes resources,organic acid extract,ethyl acetate extract and n-butanol extract were prepared respectively from the fruits of Ribes stenocarpum Maxim in the Qinghai-Tibet plateau,and their antibacterial activity and anti-fatigue activity were investigated. The results of antibacterial experiments showed that the different crude extracts in the fruits of Ribes stenocarpum Maxim had certain antibacterial effects. When the concentration was 100 mg/mL,ethyl acetate extract II had the best inhibitory effect on C.albicans,and the diameter of the inhibitory circle reached 20 mm(very sensitive),the total organic acid and total flavonoids in the crude extract were(2.30±0.1)and(2.28±0.02) mg/g,respectively. The antibacterial effect was better than the positive control(drug ampicillin,10 IU/tablet),and the MIC and MBC values for C.albicans were 1.56,3.13 mg/mL,respectively. Moreover,its antibacterial effect enhanced with the increase of the concentration of the extraction. In the anti-fatigue activity experiment,the results showed that compared with the blank control group,each dose group of ethyl acetate extract II in the fruits of Ribes stenocarpum Maxim could significantly prolong the swimming time of mice(p<0.05),improve the level of myoglycogen and liver glycogen,and enhance the activity of lactic acid dehydrogenase,at the same time,the serum content of urine nitrogen was reduced,revealing that the fruits of Ribes stenocarpum Maxim had good anti-fatigue activity and the effect was better than Rhodiola positive control group.
In order to provide a theoretical basis for the exploitation and utilization of Ribes resources,organic acid extract,ethyl acetate extract and n-butanol extract were prepared respectively from the fruits of Ribes stenocarpum Maxim in the Qinghai-Tibet plateau,and their antibacterial activity and anti-fatigue activity were investigated. The results of antibacterial experiments showed that the different crude extracts in the fruits of Ribes stenocarpum Maxim had certain antibacterial effects. When the concentration was 100 mg/mL,ethyl acetate extract II had the best inhibitory effect on C.albicans,and the diameter of the inhibitory circle reached 20 mm(very sensitive),the total organic acid and total flavonoids in the crude extract were(2.30±0.1)and(2.28±0.02) mg/g,respectively. The antibacterial effect was better than the positive control(drug ampicillin,10 IU/tablet),and the MIC and MBC values for C.albicans were 1.56,3.13 mg/mL,respectively. Moreover,its antibacterial effect enhanced with the increase of the concentration of the extraction. In the anti-fatigue activity experiment,the results showed that compared with the blank control group,each dose group of ethyl acetate extract II in the fruits of Ribes stenocarpum Maxim could significantly prolong the swimming time of mice(p<0.05),improve the level of myoglycogen and liver glycogen,and enhance the activity of lactic acid dehydrogenase,at the same time,the serum content of urine nitrogen was reduced,revealing that the fruits of Ribes stenocarpum Maxim had good anti-fatigue activity and the effect was better than Rhodiola positive control group.
引文
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[23]陈琦,王尚.鹅不食草总有机酸的提取与分析[J].中国现代药物应用,2010,4(18):10-11.
[24]邱萍,冯煦,单宇,等.分光光度法测定碱蓬中总黄酮、总皂苷的含量[J].食品研究与开发,2015,36(9):54-56.
[25]王虹玲,武婷茹,姜诗文.香蕉皮单宁的提取及其提取物的抑菌抗氧化活性[J].食品与发酵工业,2014,40(11):253-258.
[26]Shan B,Cai Y Z,Brooks J D,et al.Antibacterial properties and major bioactive components of cinnamon stick(Cinnamomum burmannii):Activity against food borne pathogenic bacteria[J].Journal of Agricultural and Food Chemistry,2007,55:5487-5490.
[27]Han L,Meng J,Yang Y,et al.Antioxidant and anti-fatigue activities of seed oil from the berries of three indigenous plants in tibetan plateau[J].Journal of Food and Nutrition Research,2015,7(3):445-457.
[28]马力.高原红景天抗疲劳作用的研究[J].中国应用生理学杂志,2014,30(1):42-47.
[29]王冲,侯娟,芮菁.复方红景天片耐缺氧及抗疲劳作用的实验评价[J].天津药学,2018,30(3):5-8.
[2]李耀阶.青海木本植物志[M].西宁:青海人民出版社,1987:223-237.
[3]王占林,樊光辉,贺永远,等.青海高原茶藨子属树种资源与育苗技术[J].北方园艺,2012(19):43-45.
[4]杨永昌,何廷农,卢生莲,等.藏药志[M].西宁:青海人民出版社,1991:404-406.
[5]江德森,牛佳牧,肖艳.吉林省长白山林区天然绿色食品资源产业化开发及模式研究[J].中国林副特产,2004(1):5-9.
[6]杨秀玲.浅谈青海省茶藨子植物分布现状及开发利用[J].青海农林科技,2011(4):68-70.
[7]陈艳平,许传军,李立柱.欧洲茶藨子属植物育种的目标、成绩与发展趋势综述[J].吉林蔬菜,2006(4):53.
[8]Tits M,Poukens P,Angenot L Y.Proanthocyanidins from Ribes nigrum leaves[J].Bulletin de Liaison-Groupe Polyphenols,1992,16(1):182-185.
[9]周霞.大黄、茶藨子等中草药活性成分的提取分离及生物活性研究[D].贵阳:贵州大学,2006.
[10]师治贤,刘梅,胡凤祖,等.青藏茶藨子种子中的脂肪酸含量分析[J].西北植物学报,2004(1):149-151.
[11]Lu Y,Yeap F L.Polyphenolic constituents of black currant seed residue[J].Food Chemistry,2003,80(1):71-76.
[12]Blech T,Budzikiewicz H,Dill D.β-Carboline alkaloids from Ribes nigrum[J].C:Bioscience,1994,49(9/10):540-544.
[13]张晶晶,马明呈,徐宗才,等.茶藨属植物研究综述[J].青海大学学报,2013,31(3):17-22.
[14]袁凤莲.茶藨子茶的药理作用及加工[J].新疆林业,2011(2):42.
[15]贾健辉,张彦丽,宋宏光.山药东北茶藨子复合饮料的研制[J].保鲜与加工,2012,12(1):36-38.
[16]杜伟伟.香茶藨子叶片中总酚酸、总黄酮提取及生物活性研究[D].哈尔滨:东北林业大学,2016.
[17]邹维娜,王微,徐启江,等.香茶藨子叶片总酚、总黄酮提取及抗氧化性研究[J].食品工业科技,2017,38(2):266-272.
[18]李静,敖亮,刘宇宏,等.五裂茶藨子叶的化学成分及其抗炎活性研究[J].华西药学杂志,2018,33(3):235-238.
[19]李洁,李晓然,宫路路,等.乳酸片球菌发酵液中主要有机酸及其抑菌性研究[J].食品与发酵工业,2014,40(5):124-129.
[20]赵建芬,卢维聪,关萍萍.酢浆草提取物抑菌活性的研究及有机酸组分的分析[J].中国食品添加剂,2017(2):93-98.
[21]程书朋,张焜,武文敬,等.簕菜叶提取物中总黄酮含量及其抑菌活性研究[J].食品研究与开发,2017,38(18):36-39.
[22]康宏玲,杨玉红,康宗利.昆仑雪菊总黄酮的提取及抑菌和抗氧化分析[J].食品科技,2018,43(6):236-241.
[23]陈琦,王尚.鹅不食草总有机酸的提取与分析[J].中国现代药物应用,2010,4(18):10-11.
[24]邱萍,冯煦,单宇,等.分光光度法测定碱蓬中总黄酮、总皂苷的含量[J].食品研究与开发,2015,36(9):54-56.
[25]王虹玲,武婷茹,姜诗文.香蕉皮单宁的提取及其提取物的抑菌抗氧化活性[J].食品与发酵工业,2014,40(11):253-258.
[26]Shan B,Cai Y Z,Brooks J D,et al.Antibacterial properties and major bioactive components of cinnamon stick(Cinnamomum burmannii):Activity against food borne pathogenic bacteria[J].Journal of Agricultural and Food Chemistry,2007,55:5487-5490.
[27]Han L,Meng J,Yang Y,et al.Antioxidant and anti-fatigue activities of seed oil from the berries of three indigenous plants in tibetan plateau[J].Journal of Food and Nutrition Research,2015,7(3):445-457.
[28]马力.高原红景天抗疲劳作用的研究[J].中国应用生理学杂志,2014,30(1):42-47.
[29]王冲,侯娟,芮菁.复方红景天片耐缺氧及抗疲劳作用的实验评价[J].天津药学,2018,30(3):5-8.