不同类型茶园土壤细菌多样性及群落结构研究
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摘要
分别选取有机茶园(OTP)、无公害茶园(NPTP)和普通茶园(CTP)的茶树根际土壤,利用Illumina Miseq高通量测序技术研究其细菌群落多样性、结构和组成,并分析茶园土壤理化性质与细菌群落结构的相关性。结果表明:有机茶园土壤的细菌群落多样性指数(Chao1)显著高于无公害和普通茶园土壤;3种茶园土壤细菌群落结构存在显著性差异,变形菌门(Proteobacteria)、酸杆菌门(Acidobacteria)和绿弯菌门(Chloroflexi)在不同茶园土壤中都属于优势菌群,但有机茶园土壤的细菌群落组成在门和属水平上与无公害和普通茶园存在显著差异;土壤有效磷(AP)、总磷(TP)和pH值是影响茶园土壤细菌群落结构的关键理化因子。
Illumina Miseq high-throughput sequencing technology was used to study the soil bacterial community diversity,structure and composition of organic tea plantation(OTP),non-polluted tea plantation(NPTP) and common tea plantation(CTP).Alpha-diversity index(Chao1) of OTP was significantly higher than that of NPTP and CTP.PCoA results showed the clear separation of bacterial communities structure in the three tea plantations soils.The results of relative bacterial abundance showed that Proteobacteria,Acidobacteria and Chloroflexi were most dominant bacteria in all tea plantation soil samples under three different agricultural management modes.The bacterial community structure of OTP was significantly different with that of NPTP and CTP in phylum and genus level.AP,TP and pH values were the key soil factors to influence the bacterial communities structure in tea plantation soils.The bacterial diversity,community structure and composition of different tea plantation soils were elaborated in this study,which provided a theoretical basis for the further studies on exploring and explaining the characteristics of microorganisms in tea plantation soils under different facility management regimes.
Illumina Miseq high-throughput sequencing technology was used to study the soil bacterial community diversity,structure and composition of organic tea plantation(OTP),non-polluted tea plantation(NPTP) and common tea plantation(CTP).Alpha-diversity index(Chao1) of OTP was significantly higher than that of NPTP and CTP.PCoA results showed the clear separation of bacterial communities structure in the three tea plantations soils.The results of relative bacterial abundance showed that Proteobacteria,Acidobacteria and Chloroflexi were most dominant bacteria in all tea plantation soil samples under three different agricultural management modes.The bacterial community structure of OTP was significantly different with that of NPTP and CTP in phylum and genus level.AP,TP and pH values were the key soil factors to influence the bacterial communities structure in tea plantation soils.The bacterial diversity,community structure and composition of different tea plantation soils were elaborated in this study,which provided a theoretical basis for the further studies on exploring and explaining the characteristics of microorganisms in tea plantation soils under different facility management regimes.
引文
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[32]杨君,周卫军,杨威,等.茶树根际与非根际土壤磷形态变化特征[J].水土保持通报,2013,33(4):216-220.
[33]Han,W.,Kemmitt,S.,Brookes,P.Soil microbial biomass and activity in Chinese tea gardens of varying stand age and productivity.Soil Biol.Biochem,2007 39:1468-1478.
[34]Han,S.H.,An,J.Y.,Hwang,J.,et al.The effects of organic manure and chemical fertilizer on the growth and nutrient concentrations of yellow poplar(Liriodendron tulipifera Lin.)in a nursery system[J].For Sci Technol,2016,3:137-143.
[35]Muktamar,Z.,Sudjatmiko,S.,Fahrurrozi,F.,et al.M.Soil Chemical Improvement under Application of Liquid Organic Fertilizer in Closed Agriculture System[J].Int J Agric Technol,2017,13:1715-1727
[36]Heinze S,Raupp J,Joergensen R G.Effects of fertilizer and spatial heterogeneity in soil pH on microbial biomass indices in a long-term field trial of organic agriculture[J].Plant and Soil,2010,328(1-2):203-215.
[37]施瑶,王忠强,张心昱,等.氮磷添加对内蒙古温带典型草原土壤微生物群落结构的影响[J].生态学报,2014,34(17):4943-4949.
[38]Cho,S.J.,Kim,M.H.,Lee,Y.O.Effect of pH on soil bacterial diversity[J].J Environ Prot Ecol,2016,40:10.
[39]Zhalnina,K.,Dias,R.,Quadros,P.D.d.,Davis-Richardson,A.,Camargo,F.A.O.,Clark,I.M.,P.McGrath,S.,Hirsch,P.R.,Triplett,E.W.Soil pH determines microbial diversity and composition in the park grass experiment[J].Mol Ecol,2015,69:395-406.
[40]Liu,J.,Sui,Y.,Yu,Z.,et al.High throughput sequencing analysis of biogeographical distribution of bacterial communities in the black soils of northeast china[J].Soil Biology and Biochemistry,2014,(70):113-122.
[2]Eisenhauer N.,Scheu S.,A.J.Bacterial diversity stabilizes community productivity[J].PloS one,2012,7:e34517.
[3]Adesemoye,AO.,Kloepper JW.Plant-microbes interactions in enhanced fertilizer-use efficiency[J].Appl Microbiol Biotechnol,2009,85:1-12.
[4]Jetiyanonal,K.,Plianbangchang,P.Potential of Bacillus cereus strain RS87 for partial replacement of chemical fertilisers in the production of Thai rice cultivars[J].J Sci Food Agric,2012,92:1080-1085.
[5]Richardson,A.E.,Barea,J.-M.,McNeill,A.M.,et al.Acquisition of phosphorus and nitrogen in the rhizosphere and plant growth promotion by microorganisms[J].Plant Soil,2009,321:305-339.
[6]宿晓琳,李英滨,杨波,等.植物多样性组成对亚热带森林土壤微生物群落的影响[J].生态学杂志,2018,37(8):29-36.
[7]侯夫云,赵兵,王庆美,等.分子生物学技术在甘薯中的应用[J].分子植物育种,2006(s1):119-122.
[8]许玲,吴魁,魏伶俐,等.基于分子生物学技术的作物种质资源创新研究现状及发展策略[J].江苏农业科学,2017,45(23):11-14.
[9]Mago,T.,Salzberg,S.L.FLASH:fast length adjustment o f s h o r t r e a d s t o i m p r o v e g e n o m e a s s e m b l i e s[J].Bioinformatics,2011,27:2957-2963.
[10]Kong,Y.Btrim:a fast,lightweight adapter and quality trimming program for next-generation sequencing technologies[J].Genomics,2011,98:152-153.
[11]Edgar,R.C.UPARSE:highly accurate OTU sequences from microbial amplicon reads[J].Nat Methods,2013,10:996.
[12]Schloss,P.D.,Westcott,S.L.,Thomas Ryabin,J.R.H.,et al.Introducing mothur:open-source,platform-independent,community-supported software for describing and comparing microbial communities[J].Appl Microbiol Biotechnol,2009,75:7537-7541.
[13]Chao,A.Nonparametric Estimation of the Number of Classes in a Population[J].Scand J Stat,1984,11:265-270.
[14]Lozupone,C.,Hamady,M.,Knight,R.UniFrac--an online tool for comparing microbial community diversity in a phylogenetic context[J].BMC bioinformatics,2006(7):371.
[15]袁红朝,秦红灵,刘守龙,等.长期施肥对红壤性水稻土细菌群落结构和数量的影响[J].中国农业科学,2011,44(22):4610-4617.
[16]徐永刚,宇万太,马强,等.长期不同施肥制度对潮棕壤微生物生物量碳、氮及细菌群落结构的影响[J].应用生态学报,2010,21(8):2078-2085.
[17]吕宁,周光海,陈云,等.滴施生物药剂对棉花生长、黄萎病防治及土壤微生物数量的影响[J].西北农业学报,2018,27(7):138-146.
[18]张丽荣,马建华,杜玉宁.不同生物制剂对黄瓜土壤微生物数量及发病率和产量的影响[J].北方园艺,2012(21):115-117.
[19]张仕颖,夏运生,肖炜,等.除草剂丁草胺对高产水稻土微生物群落功能多样性的影响[J].生态环境学报,2013,22(5):815-819.
[20]张雯雯,徐军,董丰收,等.苄嘧磺隆对水稻田土壤微生物群落功能多样性的影响[J].农业环境科学学报,2014,33(9):1749-1754.
[21]Faoro,H.,Alves,A.C.,Souza,E.M.,et al.Influence of soil characteristics on the diversity of bacteria in the Southern Brazilian Atlantic Forest[J].Appl Microbiol Biotechnol,2010,76:4744-4749.
[22]Janssen,P.H.Identifying the dominant soil bacterial taxa in libraries of 16S rRNA and 16S rRNA genes[J].Appl Microbiol Biotechnol,2010,72:1719-1728.
[23]Li,Y.C.,Li,Z.,Li,Z.W.,et al.Variations of rhizosphere bacterial communities in tea(Camellia sinensis L.)continuous cropping soil by high-throughput pyrosequencing approach[J].Journal of applied microbiology,2016,121:787-799.
[24]You,J.,Das,A.,Dolan,E.M.,et al.Ammonia-oxidizing archaea involved in nitrogen removal[J].Water research,2009,43:1801-1809.
[25]Martiny,A.C.,Albrechtsen,H.-J.,Arvin,E.et al.Identification of Bacteria in Biofilm and Bulk Water Samples from a Nonchlorinated Model Drinking Water Distribution System:Detection of a Large Nitrite-Oxidizing Population Associated with Nitrospira spp[J].Appl Microbiol Biotechnol,2005,71:8611-8617.
[26]Diacono,M.,Montemurro,F.Long-term effects of organic amendments on soil fertility.A review[J].Agron Sustain Dev,2010,30:401-422.
[27]Manna,M.C.,Swarup,A.,Wanjari,R.H.,et al.Long-term fertilization,manure and liming effects on soil organic matter and crop yields[J].Soil Tillage Res,2007,94:397-409.
[28]Sapp,M.,Harrison,M.,Hany,et al.Comparing the effect of digestate and chemical fertiliser on soil bacteria[J].Appl Soil Ecol,2015,86:1-9.
[29]张敬业,张文菊,徐明岗,等.长期施肥下红壤有机碳及其颗粒组分对不同施肥模式的响应[J].植物营养与肥料学报,2012,18(4):869-876.
[30]陈贵,赵国华,张红梅,等.长期施用有机肥对水稻产量和氮磷养分利用效率的影响[J].中国土壤与肥料,2017(1):92-97.
[31]吴志丹,尤志明,江福英,等.配施有机肥对茶园土壤性状及茶叶产质量的影响[J].土壤,2015,47(05):874-879.
[32]杨君,周卫军,杨威,等.茶树根际与非根际土壤磷形态变化特征[J].水土保持通报,2013,33(4):216-220.
[33]Han,W.,Kemmitt,S.,Brookes,P.Soil microbial biomass and activity in Chinese tea gardens of varying stand age and productivity.Soil Biol.Biochem,2007 39:1468-1478.
[34]Han,S.H.,An,J.Y.,Hwang,J.,et al.The effects of organic manure and chemical fertilizer on the growth and nutrient concentrations of yellow poplar(Liriodendron tulipifera Lin.)in a nursery system[J].For Sci Technol,2016,3:137-143.
[35]Muktamar,Z.,Sudjatmiko,S.,Fahrurrozi,F.,et al.M.Soil Chemical Improvement under Application of Liquid Organic Fertilizer in Closed Agriculture System[J].Int J Agric Technol,2017,13:1715-1727
[36]Heinze S,Raupp J,Joergensen R G.Effects of fertilizer and spatial heterogeneity in soil pH on microbial biomass indices in a long-term field trial of organic agriculture[J].Plant and Soil,2010,328(1-2):203-215.
[37]施瑶,王忠强,张心昱,等.氮磷添加对内蒙古温带典型草原土壤微生物群落结构的影响[J].生态学报,2014,34(17):4943-4949.
[38]Cho,S.J.,Kim,M.H.,Lee,Y.O.Effect of pH on soil bacterial diversity[J].J Environ Prot Ecol,2016,40:10.
[39]Zhalnina,K.,Dias,R.,Quadros,P.D.d.,Davis-Richardson,A.,Camargo,F.A.O.,Clark,I.M.,P.McGrath,S.,Hirsch,P.R.,Triplett,E.W.Soil pH determines microbial diversity and composition in the park grass experiment[J].Mol Ecol,2015,69:395-406.
[40]Liu,J.,Sui,Y.,Yu,Z.,et al.High throughput sequencing analysis of biogeographical distribution of bacterial communities in the black soils of northeast china[J].Soil Biology and Biochemistry,2014,(70):113-122.