乳腺癌根治术患者miRNA-23a表达水平与术后复发转移的关系
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摘要
目的探讨乳腺癌根治术患者miRNA-23a表达水平与术后复发转移的关系。结果选取2013年3月至2016年3月本院收治的乳腺癌根治术患者160例,依据2年内复发转移情况分为复发组(n=50例)和未发组(n=110例),采用实时荧光定量聚合酶联反应法(RT-PCR)检测miRNA-23a表达水平,分析miRNA-23a表达水平与术后复发转移的关系。结果单因素分析结果显示,复发组和未发组肿瘤类型、直径、TNM分期、淋巴结转移、雌激素受体(ER)和孕激素受体(PR)、miRNA-23a表达水平比较,差异有统计学意义(P<0.05);Logistic回归性分析显示,在校正肿瘤类型、直径、TNM分期、淋巴结转移、ER和PR等因素后,miRNA-23a表达水平是患者术后复发转移的独立影响因素(P<0.05);ROC曲线显示,miRNA-23a表达水平以<1.0评估患者术后复发转移的敏感度、特异度、准确度、曲线下面积为92.00%、96.36%、95.00%、0.882,一致性良好(P<0.05)。结论乳腺癌根治术患者miRNA-23a表达水平与术后复发转移的关系密切,其低表达水平可能提示患者术后复发转移,检测其表达水平可作为评估患者术后复发转移的重要指标。
Objective To discuss the relationship between miRNA-23 aexpression level and postoperative recurrence metastasis in patients undergoing breast cancer radical mastectomy.Methods 160 patients undergoing breast cancer radical mastectomy were selected from March 2013 to March 2016 in our Hospital,according to the recurrence metastasis within 2 years,they were divided into the recurrence group(n=50)and the non recurrence group(n=110),the miRNA-23 aexpression level were detected by real time fluorescence quantitative polymerase chain reaction(RT-PCR),and the relationship between the miRNA-23 aexpression level and the postoperative recurrence metastasis was analyzed.Results The single factor analysis result showed that,the comparison of the tumor type,diameter,TNM staging,lymph node metastasis,estrogen receptor(ER)and progesterone receptor(PR)and miRNA-23 aexpression level in the recurrence group and the non recurrence group,the difference was statistically significant(P<0.05).The Logistic regression analysis showed that,the tumor type,diameter,TNM staging,lymph node metastasis,ER and PR were corrected,the miRNA-23 aexpression level was an independent factor for the postoperative recurrence metastasis of patients(P<0.05).The ROC curve showed that,the sensitivity,specificity,accuracy,area under the curve of miRNA-23 a expression level<1.0 for evaluating the postoperative recurrence metastasis of patients were 92.00%,96.36%,95.00%,0.882,it had good consistency(P<0.05).Conclusion The miRNA-23 aexpression level in patients undergoing breast cancer radical mastectomy is closely related to postoperative recurrence metastasis,its low expression level may indicate the postoperative recurrence and metastasis of patients,the detection of its expression level can be used as the important index for evaluating the postoperative recurrence metastasis of patients.
Objective To discuss the relationship between miRNA-23 aexpression level and postoperative recurrence metastasis in patients undergoing breast cancer radical mastectomy.Methods 160 patients undergoing breast cancer radical mastectomy were selected from March 2013 to March 2016 in our Hospital,according to the recurrence metastasis within 2 years,they were divided into the recurrence group(n=50)and the non recurrence group(n=110),the miRNA-23 aexpression level were detected by real time fluorescence quantitative polymerase chain reaction(RT-PCR),and the relationship between the miRNA-23 aexpression level and the postoperative recurrence metastasis was analyzed.Results The single factor analysis result showed that,the comparison of the tumor type,diameter,TNM staging,lymph node metastasis,estrogen receptor(ER)and progesterone receptor(PR)and miRNA-23 aexpression level in the recurrence group and the non recurrence group,the difference was statistically significant(P<0.05).The Logistic regression analysis showed that,the tumor type,diameter,TNM staging,lymph node metastasis,ER and PR were corrected,the miRNA-23 aexpression level was an independent factor for the postoperative recurrence metastasis of patients(P<0.05).The ROC curve showed that,the sensitivity,specificity,accuracy,area under the curve of miRNA-23 a expression level<1.0 for evaluating the postoperative recurrence metastasis of patients were 92.00%,96.36%,95.00%,0.882,it had good consistency(P<0.05).Conclusion The miRNA-23 aexpression level in patients undergoing breast cancer radical mastectomy is closely related to postoperative recurrence metastasis,its low expression level may indicate the postoperative recurrence and metastasis of patients,the detection of its expression level can be used as the important index for evaluating the postoperative recurrence metastasis of patients.
引文
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[3]Lee J,Galloway R,Grandjean G,et al.Comprehensive two-and three-dimensional RNAi screening identifies PI3Kinhibition as a complement to MEK inhibitor AS703026for combination treatment of triplenegative breast cancer[J].J Cancer,2015,6(12):1306.
[4]聂明月,杨晓葵.miR-23a和miR-27a在卵巢中的生理与病理作用[J].国际生殖健康/计划生育杂志,2014,33(5):367.
[5]周嘉彬,黄少隆,吕钰冰,等.microRNA与cadherin 1基因在乳腺癌侵袭转移中的调控机制[J].生物医学工程与临床,2017,21(4):447.
[6]Zhang R,Li Y,Dong X,et al.miR-363sensitizes cisplatin-induced apoptosis targeting in Mel-1in breast cancer[J].Med Oncol,2014,31(12):347.
[7]De-Santis C,Ma J,Bryan L,et al.Breast cancer statistics,2013[J].CA Cancer J Clin,2014,64(1):52.
[8]陈达丰,周松,张雪惠,等.男性乳腺癌的临床特点和术后生存质量影响因素分析[J].中国实验诊断学,2017,21(10):1696.
[9]Pan Y,Wang R,Zhang F,et al.MicroRNA-130ainhibits cell proliferation,invasion and migration in human breast cancer by targeting the RAB5A[J].Int J Clin Exp Pathol,2015,8(1):384.
[10]陈军.老年女性乳腺癌患者人表皮生长因子受体-2、抑癌基因、雌激素受体和孕激素受体表达情况及临床治疗[J].中国老年学杂志,2016,36(9):2181.
[11]魏宁,王萍,王斐,等.微小RNA-205在甲状腺乳头状癌中的表达及临床意义[J].现代生物医学进展,2016,16(11):2141.
[12]Shaker O,Maher M,Nassar Y,et al.Role of microRNAs-29b-2,-155,-197and-205as diagnostic biomarkers in serum of breast cancer females[J].Gene,2015,560(1):77.
[13]Iwakawa HO,Tomari Y.The functions of microRNAs:mRNAdecay and translational repression[J].Trends Cell Biol,2015,25(11):651.
[14]何嵘,马业罡,刘宏旭.miR-23a/b在非小细胞肺癌的表达及临床意义[J].解剖科学进展,2017,23(1):61.
[15]Zhu Y,Wang D,Wang F,et al.A comprehensive analysis of GA-TA-l-regulated miRNAs reveals miR-23ato be a positive modulator of erythropoiesis[J].Nucleic Acids Res,2013,41(7):4129.
[16]王伟,刘建波,李会荣,等.TWIST和雌激素受体α在乳腺癌组织中的表达及对预后的影响[J].中国现代医学杂志,2015,25(21):11.
[17]Clarice P,Silvia S,Antonella T,et al.Polymorphisms in base excision repair genes:breast cancer risk and individual radiosensitivity[J].World Journal of Clinical Oncology,2014,5(5):874.