水文浮标法在黏菌原生质流测速中的应用
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摘要
黏菌显型原质团可以作为研究胞质环流的模式材料,其大型、具有稳定的外质和多泡流动的内质结构,使得对其原生质流的观察、测速变得十分方便。文中描述了一种借助水文浮标测定黏菌显型原质团原生质流流速的方法,通过随机测量流经菌脉的9个气泡,获得气泡流速为25. 83～289. 05μm/s,9个气泡的平均流速为190. 01μm/s,这个数值可以认定为这段菌脉原生质流的流速。这种方法有助于对胞质环流进行定量研究。
Phaneroplasmodium of myxomycetes can be served as a model material for studies on cyclosis. Because of its stabilized ectoplasm,bubbly motile endoplasm and large size,the observation and measurement of the velocity of its protoplasmic streaming became easier. Hydrological buoy method to measure the velocity of streaming was described in this paper. The results showed that the streaming velocity among 9 bubbles selected randomly varied from 25. 83 μm/s to 289. 05 μm/s,and the average velocity was 190. 01 μm/s.
Phaneroplasmodium of myxomycetes can be served as a model material for studies on cyclosis. Because of its stabilized ectoplasm,bubbly motile endoplasm and large size,the observation and measurement of the velocity of its protoplasmic streaming became easier. Hydrological buoy method to measure the velocity of streaming was described in this paper. The results showed that the streaming velocity among 9 bubbles selected randomly varied from 25. 83 μm/s to 289. 05 μm/s,and the average velocity was 190. 01 μm/s.
引文
[1] Kamiya. The protoplasmic flow of the myxomycete plasmodium as revealed by a volumetric analysis[J]. Protoplasma,1950,39:344-357.
[2] Kuroda K,Kamiya N. Active movement of Nitella chloroplasts in vitro[M]∥Proceeding Japan Academy. Osaka:Academic Press,1975:774-777.
[3] Shimmen T. Dependency of cytoplasmic streaming on intracellular ATP and Mg2+concentrations[J]. Cell Structure and Function,1978,3:113-121.
[4] Tominaga Y,Tazawa M. Reversible inhibition of cytoplasmic streaming by intracellular Ca2+in tonoplast-free cells of Chara australis[J]. Protoplasma,1981,109:103-111.
[5] Shimmen T,Tazawa M. Cytoplasmic streaming in the cell model of Nitella[J]. Protoplasma,1982,112:101-106.
[6] Nothnagel E A,Barak L S,Sanger J W,et al. Fluorescence studies on modes of cytochalasin B and phallotoxin action on cytoplasmic streaming in Chara[J]. The Journal of Cell Biology,1981,88:364-372.
[7]蒋敏.几种观察细胞质运动的材料[J].生物学通报,1988,7:43.
[8]刘箭,王秀珍,陈大东.植物微管特效解聚剂APM对紫露草雄蕊毛细胞胞质环流的影响[J].植物生理学报,1992,18(4):348-354.
[9] Alexopoulos C J. Gross morphology of the plasmodium and its possible significance in the relationships among the myxomycetes[J]. Mycologia,1960,52:1-20.
[10]王晓丽,李艳双,李玉.几种黏菌显型原质团培养研究[J].吉林农业大学学报,2005,27(2):140-143.
[11]王晓丽,李玉.几种黏菌原生质团蛋白质和DNA含量的测定[J].吉林农业大学学报,2005,27(4):366-368.
[12] Wohlfarth-Bottermann K E,Fleischer M. Cycling aggregation patterns of cytoplasmic F-actin coordinated with oscillating tension force generation[J]. Cell Tissue Research,1976,165:327-344.
[13]吕冬霞,蔚荣海,李景鹏,等.淡黄绒泡菌和全白绒泡菌原生质流向的观察[J].菌物学报,2012,31(6):952-955.
[14]刘建军,何志江,李白羽,等.图像法水面流速检测方法简介[J].水文,2003,23(6):53-58,61.
[15]张振,徐立中,樊棠怀,等.河流水面成像测速方法的比测试验研究[J].水利信息化,2014(5):31-41.
[16] Tominaga M,Kimura A,Yokota E,et al. Cytoplasmic streaming velocity as a plant size determinant[J]. Developmental Cell,2013(27):345-352.
[17]蒋世翠,张波,李艳双,等.基于GLME/GC-MS分析黄柄钙皮菌的挥发性成分[J].菌物研究,2017,15(4):255-258,270.
[18]高扬,闫淑珍,王高伟,等.河南宝天曼自然保护区黏菌初报[J].菌物研究,2018,16(3):170-181.
[19]秦立羽玮,秦问敏.微孢子虫的进化起源与系统分类现状[J].菌物研究,2018,16(2):106-114.
[20]杨天雪,李晨,李艳双,等.4种黏菌黏变形体运动中的形态变化[J].吉林农业大学学报,2018,40(2):178-184.
[21]李晨,王晓丽,王晓丽,等.几种黏菌黏变形体形态及运动特征[J].菌物学报,2013,32(5):913-918.
[2] Kuroda K,Kamiya N. Active movement of Nitella chloroplasts in vitro[M]∥Proceeding Japan Academy. Osaka:Academic Press,1975:774-777.
[3] Shimmen T. Dependency of cytoplasmic streaming on intracellular ATP and Mg2+concentrations[J]. Cell Structure and Function,1978,3:113-121.
[4] Tominaga Y,Tazawa M. Reversible inhibition of cytoplasmic streaming by intracellular Ca2+in tonoplast-free cells of Chara australis[J]. Protoplasma,1981,109:103-111.
[5] Shimmen T,Tazawa M. Cytoplasmic streaming in the cell model of Nitella[J]. Protoplasma,1982,112:101-106.
[6] Nothnagel E A,Barak L S,Sanger J W,et al. Fluorescence studies on modes of cytochalasin B and phallotoxin action on cytoplasmic streaming in Chara[J]. The Journal of Cell Biology,1981,88:364-372.
[7]蒋敏.几种观察细胞质运动的材料[J].生物学通报,1988,7:43.
[8]刘箭,王秀珍,陈大东.植物微管特效解聚剂APM对紫露草雄蕊毛细胞胞质环流的影响[J].植物生理学报,1992,18(4):348-354.
[9] Alexopoulos C J. Gross morphology of the plasmodium and its possible significance in the relationships among the myxomycetes[J]. Mycologia,1960,52:1-20.
[10]王晓丽,李艳双,李玉.几种黏菌显型原质团培养研究[J].吉林农业大学学报,2005,27(2):140-143.
[11]王晓丽,李玉.几种黏菌原生质团蛋白质和DNA含量的测定[J].吉林农业大学学报,2005,27(4):366-368.
[12] Wohlfarth-Bottermann K E,Fleischer M. Cycling aggregation patterns of cytoplasmic F-actin coordinated with oscillating tension force generation[J]. Cell Tissue Research,1976,165:327-344.
[13]吕冬霞,蔚荣海,李景鹏,等.淡黄绒泡菌和全白绒泡菌原生质流向的观察[J].菌物学报,2012,31(6):952-955.
[14]刘建军,何志江,李白羽,等.图像法水面流速检测方法简介[J].水文,2003,23(6):53-58,61.
[15]张振,徐立中,樊棠怀,等.河流水面成像测速方法的比测试验研究[J].水利信息化,2014(5):31-41.
[16] Tominaga M,Kimura A,Yokota E,et al. Cytoplasmic streaming velocity as a plant size determinant[J]. Developmental Cell,2013(27):345-352.
[17]蒋世翠,张波,李艳双,等.基于GLME/GC-MS分析黄柄钙皮菌的挥发性成分[J].菌物研究,2017,15(4):255-258,270.
[18]高扬,闫淑珍,王高伟,等.河南宝天曼自然保护区黏菌初报[J].菌物研究,2018,16(3):170-181.
[19]秦立羽玮,秦问敏.微孢子虫的进化起源与系统分类现状[J].菌物研究,2018,16(2):106-114.
[20]杨天雪,李晨,李艳双,等.4种黏菌黏变形体运动中的形态变化[J].吉林农业大学学报,2018,40(2):178-184.
[21]李晨,王晓丽,王晓丽,等.几种黏菌黏变形体形态及运动特征[J].菌物学报,2013,32(5):913-918.