维药卡克乃其露剂对实验性大鼠慢性前列腺炎的保护作用
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摘要
目的观察维药卡克乃其露剂对非细菌性前列腺炎大鼠模型的保护作用并探讨其治疗慢性前列腺炎的作用机制。方法本研究采用腹部皮下多点注射大鼠前列腺组织抗原的方法复制非细菌性前列腺炎大鼠模型,40只Wistar大鼠随机分为5组,通过检测大鼠体重、前列腺湿重、白细胞和卵磷脂数目、和对IL-8,IL-10,TNF-α等细胞因子的影响,通过病理组织切片评价维药卡克乃其的抗前列腺炎的作用。结果与模型组比较,维药卡克乃其均有使白细胞数明显的降低而卵磷脂小体数目生升高(P<0.05~0.01)。ELISA结果显示,维药卡克乃其露剂中、高剂量组抑制了大鼠动物血清中IL-8、TNF-α水平(P<0.05~0.01),但模型组与给药组低,中剂量组IL-10水平没有明显的差异(P>0.05)而高剂量组IL-10水平明显的高于模型组(P<0.05),病理组织学研究结果显示维药卡克乃其露剂高剂量组有效的缓解大鼠前列腺炎症状态。结论维药卡克乃其露剂能够明显减轻模型大鼠前列腺自身免疫性炎症反应。
Objective To evaluate the anti-prostatitis effects of Erki Kaknaq on Chronic non bacterial prostatitis by observing which effects on Rat model. Methods 40 male Wistar rats were assigned randomly into five groups. All groups were made experimental autoimmune prostatitis rat model by injecting SC purified prostate protein with FCA except normal group. The anti-prostatitis effect and mechanism of Erki Kaknaq were evaluated by prostate weight,density of lecithin corpuscles(DLCC),white blood cell count(WBC),and prostatic histo-morphological parameters and by measuring IL-8,IL-10 and TNF-a level by ELISA kit using experimental model in rats Results compared with model group,the WBC number significantly decreased while DLCC were significantly increases in middle and high dosage of Erki Kaknaq(P<0.05~0.01).ELISA analysis show that compared to Model group middle and high dosage of Erki Kaknaq inhibited the IL-8、TNF-α level(P <0.05 ~0.01).but there is no significant difference in the IL-10 level between Model group and low and middle Erki Kaknaq treatment group but with high dosage group(P<0.05).Conclusion Erki Kaknaq demonstrated a protective effects on prostatitis rat model by ameliorating inflammation.
Objective To evaluate the anti-prostatitis effects of Erki Kaknaq on Chronic non bacterial prostatitis by observing which effects on Rat model. Methods 40 male Wistar rats were assigned randomly into five groups. All groups were made experimental autoimmune prostatitis rat model by injecting SC purified prostate protein with FCA except normal group. The anti-prostatitis effect and mechanism of Erki Kaknaq were evaluated by prostate weight,density of lecithin corpuscles(DLCC),white blood cell count(WBC),and prostatic histo-morphological parameters and by measuring IL-8,IL-10 and TNF-a level by ELISA kit using experimental model in rats Results compared with model group,the WBC number significantly decreased while DLCC were significantly increases in middle and high dosage of Erki Kaknaq(P<0.05~0.01).ELISA analysis show that compared to Model group middle and high dosage of Erki Kaknaq inhibited the IL-8、TNF-α level(P <0.05 ~0.01).but there is no significant difference in the IL-10 level between Model group and low and middle Erki Kaknaq treatment group but with high dosage group(P<0.05).Conclusion Erki Kaknaq demonstrated a protective effects on prostatitis rat model by ameliorating inflammation.
引文
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[13]黄志洪,袁少英,覃湛.慢性非细菌性前列腺炎患者前列腺液TNF-a、IL-8的检测及临床意义[J].中国中医药导报,2009,6(14)22-24.
[14]Mao XX,Miao GX,Yu HL,Liu CJ.Advances of studies on Zihuadiding(Herba Violae Philippicae)[J].Chengde Yi Xue Yuan Xue Bao 2010,27(3):302-305.
[15]Song Guohong,Zhang Qiumei,Pang Baozhen,Effects of different Chinese herbal prescriptions on cytokines in autoimmune prostatitis rats[J].Journal of Traditional Chinese Medicine.2015,35(2):211-217.
[16]Miller,L.J,Fischer,K A.,Goralnick,S.J et al.Interleukin-10 levels in seminal plasma:implications for chronic prostatitis-chronic pelvic pain syndrome[J].The Journal of Urology.2002,167,753-756.
[17]李宏军,商学军,黄宇烽.IL-10、IL-8在慢性前列腺炎中的改变及意义[J].中华男科学杂志,2004,10(7):486-48.
[18]洪晓华,刘建勋.对试验性非细菌性前列腺炎炎症疼痛及利尿动物模型的作用[J].中国实验方剂学杂志,2010,16(12):105-109.
[19]斯拉甫·艾白,买尼沙·买买提,阿里甫·恩提,等.维药新药治疗慢性前列腺炎的临床研究指导原则[J].中药药理与临床,2015;31(3).
[2]Shinsuke Takechi,Masayoshi Yokoyama,Nozomu Tanji,et al.Nonbacterial prostatitis caused by partial urethral obstruction in the rat[J].Urol Res.1999,27:346-350.
[3]Schneider H,Ludwing M,Hossain HM,Diemer T,et al.the 2001 Giessen Cohort study on patients with prostatitis syndrome an evaluation of inflammatory status and search for microorganism 10 years after a first analysis[J].Andologia.2003,35:258-265.
[4]Shaun Wen,Hue Lee,Kah Hay Yuen,et al.Acupuncture and immune function in chronic prostatitis/chronic pelvic pain syndrome:A randomized,controlled study[J].Complementary Therapies in Medicine.2014,22,965.
[5]Chuang S,Huang CC,Lin HC,et al.Chronic Prostatitis and depressive disorder:A three year population based study Journal of Affective Disorders.2011,134:404-409.
[6]Steenkamp V,Gouws M.C Gulumian M,Elgorashi,et al.Studies on antibacterial anti-inflammatory and antioxidant activity of herbal remedies used in the treatment of benign prostatic hyperplasia and prostatitis[J].Journal of Ethnopharmacology.2006,103:71-75.
[7]武海燕,索全伶,李俊.药用植物酸浆的研究进展[J].内蒙古石油化工.2007,2:5-9.
[8]Diana V.Kouiavskaia,Scott Southwood,Carla A.Berard,et al.T-Cell Recognition of Prostatic Peptides in Men With Chronic Prostatitis/Chronic Pelvic Pain Syndrome[J].The Jornal of Urology.2009,182,2483-2489.
[9]Nickel JC.Treatment of chronic prostatitis/chronic pelvic pain syndrome[J].International Journal of Antimicrobial Agents,2008.31,112-116.
[10]Persson BE,Ronquist G.Evidence for a mechanisticassociation between nonbacterial prostatitis and levels of urateand creatinine in expressed prostatic secretion[J].J Urol.1996,155:958.
[11]Motrich RD,Maccioni M,Molina R et al.Presence of INFgamma-secreting lymphocytes specific to prostate antigens in a group of chronic prostatitis patients[J].Clin Immunol.2005,116(2):149-157.
[12]Hochreiter WW,Weidner W.Prostatitis-a frequently unrecognized disease[J].Ther Umsch,2006,63(2):117-121.
[13]黄志洪,袁少英,覃湛.慢性非细菌性前列腺炎患者前列腺液TNF-a、IL-8的检测及临床意义[J].中国中医药导报,2009,6(14)22-24.
[14]Mao XX,Miao GX,Yu HL,Liu CJ.Advances of studies on Zihuadiding(Herba Violae Philippicae)[J].Chengde Yi Xue Yuan Xue Bao 2010,27(3):302-305.
[15]Song Guohong,Zhang Qiumei,Pang Baozhen,Effects of different Chinese herbal prescriptions on cytokines in autoimmune prostatitis rats[J].Journal of Traditional Chinese Medicine.2015,35(2):211-217.
[16]Miller,L.J,Fischer,K A.,Goralnick,S.J et al.Interleukin-10 levels in seminal plasma:implications for chronic prostatitis-chronic pelvic pain syndrome[J].The Journal of Urology.2002,167,753-756.
[17]李宏军,商学军,黄宇烽.IL-10、IL-8在慢性前列腺炎中的改变及意义[J].中华男科学杂志,2004,10(7):486-48.
[18]洪晓华,刘建勋.对试验性非细菌性前列腺炎炎症疼痛及利尿动物模型的作用[J].中国实验方剂学杂志,2010,16(12):105-109.
[19]斯拉甫·艾白,买尼沙·买买提,阿里甫·恩提,等.维药新药治疗慢性前列腺炎的临床研究指导原则[J].中药药理与临床,2015;31(3).