不同钼积累水平大豆品种苗期碳氮代谢生理差异
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摘要
为探究不同钼积累能力的大豆品种对缺钼条件下生理代谢的差异,以品种1301(钼高积累)和品种1304(钼低积累)为材料,在营养液培养下研究了两个品种光合碳代谢以及氮代谢生理差异。结果表明:缺钼降低了大豆叶片光合碳代谢能力。缺钼条件下,品种1301叶片光合速率可溶性糖、叶绿素a、叶绿素b以及叶绿素a+b含量显著高于1304,表明1301具有较高的光合碳代谢能力。缺钼也降低了根中氮代谢能力。缺钼条件下,品种1301中硝酸还原酶活性和铵态氮含量均显著高于品种1304,表明1301具有较强的氮代谢能力。以上结果表明大豆钼高积累品种能累积较多的钼,且表现出较高的碳氮代谢能力。因此认为培育大豆钼高积累品种是提高大豆生理代谢能力的一种经济有效的措施。
To explore the differences of Mo utilization efficient between high Mo accumulation and low Mo accumulation cultivars,we analyzed the physiological differences of photosynthetic carbon and nitrogen metabolism between high-Mo-accumulation and low-Mo-accumulation soybean cultivars. The results indicated that chlorophyll content,Pn,soluble sugar and shoot dry matter decreased under Mo deficient condition. Under Mo deficient condition,the chlorophyll content,Pn,soluble sugar in 1301 were significantly higher than those in 1304.These results suggested that 1301( Mo-efficient cultivar) exhibited higher carbon metabolism ability. Meanwhile,the nitrate content and nitrate/ammonium ratio increased under Mo deficient condition,and NR,GOGAT,GS activity and NH4+-N content decreased under Mo deficient condition. Under Mo deficient condition,NR activity was significantly higher in 1301 than that in 1304,which indicated 1301 exhibited a higher nitrogen assimilation ability than 1304. These results indicated that the high-Mo-accumulation soybean cultivars accumulated high Mo which enhanced the high carbon and nitrogen utilization ability. Thus,breeding high Mo accumulation soybean cultivars could efficiently improve Mo utilization of soybean.
To explore the differences of Mo utilization efficient between high Mo accumulation and low Mo accumulation cultivars,we analyzed the physiological differences of photosynthetic carbon and nitrogen metabolism between high-Mo-accumulation and low-Mo-accumulation soybean cultivars. The results indicated that chlorophyll content,Pn,soluble sugar and shoot dry matter decreased under Mo deficient condition. Under Mo deficient condition,the chlorophyll content,Pn,soluble sugar in 1301 were significantly higher than those in 1304.These results suggested that 1301( Mo-efficient cultivar) exhibited higher carbon metabolism ability. Meanwhile,the nitrate content and nitrate/ammonium ratio increased under Mo deficient condition,and NR,GOGAT,GS activity and NH4+-N content decreased under Mo deficient condition. Under Mo deficient condition,NR activity was significantly higher in 1301 than that in 1304,which indicated 1301 exhibited a higher nitrogen assimilation ability than 1304. These results indicated that the high-Mo-accumulation soybean cultivars accumulated high Mo which enhanced the high carbon and nitrogen utilization ability. Thus,breeding high Mo accumulation soybean cultivars could efficiently improve Mo utilization of soybean.
引文
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[20]秦世玉,孙学成,胡承孝,等.硫硒钨对钼酸盐吸收及钼酸盐转运子的影响[J].华北农学报,2015,30(5):232-238.
[21]李合生.植物生理生化实验原理和技术[M].北京:高等教育出版社,2000. 195-197.
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[23] Commichau F M,Forchhammer K,Stulke J. Regulatorylinks between carbon and nitrogen metabolism[J]. CurrOpin Microbiol,2006,9(2):167-172.
[24] Li P W,Yang L,Li T Y. Effects of molybdenum on ni-trogen metabolism of sugarcane[J]. Sugar Tech,2007,9(1):36-42.
[25]门中华,李生秀.钼对冬小麦硝态氮代谢的影响[J].植物营养与肥料学报,2005,11(2):205-210.
[26]刘利,张蕊,杨超,等.叶面喷施钼肥对草莓幼苗氮代谢关键酶活性与15N吸收、分配及利用的影响[J].植物生理学报,2016(7):1 035-1 044.
[27]秦亚光,王留兴,樊青霞,等.施钼对烤烟硝酸盐和亚硝酸盐含量的影响[J].河南农业科学,2008,37(7):54-56.
[2] Schwarz G,Mendel R R. Molybdenum cofactor biosyn-thesis and molybdenum enzymes[J]. Annu Rev Plant Bi-ol,2006,57(1):623-647.
[3] Kovacs B,Puskaspreszner A,Huzsvai L,et al. Effect ofmolybdenum treatment on molybdenum concentration andnitrate reduction in maize seedlings[J]. Plant PhysiolBioch,2015,96:38-44.
[4] Tischner R. Nitrate uptake and reduction in higher andlower plants[J]. Plant Cell Environ,2000,23(10):1 005-1 024.
[5] Liu H,Hu C,Sun X,et al. Interactive effects of molyb-denum and phosphorus fertilizers on photosynthetic char-acteristics of seedlings and grain yield of Brassica napus[J]. Plant Soil,2010,326:345-353.
[6]刘红恩,胡承孝,聂兆君,等.酸性黄棕壤中钼磷配施对甘蓝型油菜苗期碳氮代谢的影响[J].中国油料作物学报,2012,34(1):62-68.
[7]许嘉阳,胡承孝,孙学成.烤烟施钼对含钼酶和碳氮代谢相关酶基因表达的影响[J].植物生理学报,2016(7):1 052-1 058.
[8]李章海,宋泽民,黄刚,等.缺钼烟田施钼对烟草光合作用和氮代谢及烟叶品质的影响[J].烟草科技,2008(11):56-58.
[9]许振柱,周广胜.植物氮代谢及其环境调节研究进展[J].应用生态学报,2004,15(3):511-516.
[10] Oaks A,Hirel B. Nitrogen metabolism in roots[J]. An-nu Rev Plant Biol,1985,36(1):345-365.
[11] Li L,Wei X,Ling L,et al. Effect of exogenously ap-plied molybdenum on its absorption and nitrate metabo-lism in strawberry seedlings[J]. Plant Physiol Bioch,2017,115:200-211.
[12]喻敏,胡承孝,王运华.缺钼对冬小麦不同品种叶绿素含量和叶绿体超微结构的影响[J].华中农业大学学报,2005,24(5):465-469.
[13]庞静,胡承孝,王运华,等.钼对黄棕壤上冬小麦碳代谢的影响[J].华中农业大学学报,2001,20(1):33-35.
[14]秦世玉,孙学成,胡承孝,等.钼肥对甘蓝型油菜薹期碳氮代谢的影响[J].华北农学报,2016,31(4):227-232.
[15]刘鹏,杨玉爱.钼、硼对大豆氮代谢的影响[J].植物营养与肥料学报,1999,5(4):347-351.
[16]刘鹏,杨玉爱.钼、硼对大豆光合效率的影响[J].植物营养与肥料学报,2003,9(4):456-461.
[17]李俊,张春雷,秦岭,等.不同磷效率基因型油菜对低磷胁迫的生理响应[J].中国油料作物学报,2010,32(2):222-228.
[18]王振华,潘媛,杨露,等.两种不同硼效率甘蓝型油菜在低硼胁迫下抗氧化力差异的研究[EB/OL].北京:中国科技论文在线[2011-05-20].
[19]徐守俊,孙学成,胡承孝,等.不同大豆品种钼富集因素的主成分分析和聚类分析[J].华北农学报,2016,31(S1):399-404.
[20]秦世玉,孙学成,胡承孝,等.硫硒钨对钼酸盐吸收及钼酸盐转运子的影响[J].华北农学报,2015,30(5):232-238.
[21]李合生.植物生理生化实验原理和技术[M].北京:高等教育出版社,2000. 195-197.
[22]孙学成.钼提高冬小麦抗寒力的生理基础及分子机制[D].武汉:华中农业大学,2006.
[23] Commichau F M,Forchhammer K,Stulke J. Regulatorylinks between carbon and nitrogen metabolism[J]. CurrOpin Microbiol,2006,9(2):167-172.
[24] Li P W,Yang L,Li T Y. Effects of molybdenum on ni-trogen metabolism of sugarcane[J]. Sugar Tech,2007,9(1):36-42.
[25]门中华,李生秀.钼对冬小麦硝态氮代谢的影响[J].植物营养与肥料学报,2005,11(2):205-210.
[26]刘利,张蕊,杨超,等.叶面喷施钼肥对草莓幼苗氮代谢关键酶活性与15N吸收、分配及利用的影响[J].植物生理学报,2016(7):1 035-1 044.
[27]秦亚光,王留兴,樊青霞,等.施钼对烤烟硝酸盐和亚硝酸盐含量的影响[J].河南农业科学,2008,37(7):54-56.