接种AM真菌对煤矿区废弃土壤不同磷素形态的影响
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摘要
【目的】本文筛选出优质AM真菌,来提高矿区土壤肥力与磷素有效性。【方法】采用盆栽实验研究了山西省介休市金山坡煤矿矿区复垦土壤中分别接种根内球囊霉(Glomus intraradices,G.i)、摩西球囊霉(Glomus mosseae,G.m)、幼套球囊霉(Glomus etunicatum,G.e)与不接种丛枝菌根真菌(No inoculation arbuscular mycorrhizal fungus,NAMF)对矿区废弃土壤中玉米侵染效应、Olsen-P含量及Hedley磷形态的影响。【结果】接种G.m显著提高了根系丛枝丰富度、侵染率和侵染密度,促进菌丝结构与孢子的形成与生长。同时接种G.m显著提高了土壤Olsen-P、H_2O-P、NaHCO_3-P、NaOH-P含量;可见,接种G.m促进了土壤中不同形态磷的形成,土壤养分活化,提高了土壤的磷素利用率。HCl-P与残渣磷结构稳定,接种AMF对较不接菌处理无明显差异。结合菌根侵染与土壤中有效磷,Hedley磷形态中各组分磷含量,得出在煤矿区废弃土壤中,接种不同的丛枝菌根真菌(Arbuscular mycorrhizal fungus,AMF)对玉米侵染效应与Hedley磷形态中各组分含量的贡献各不相同,接种G.m对玉米侵染效应和Hedley磷形态各组分的贡献最大。【结论】接种摩西球囊霉(Glomus mosseae,G.m)对玉米侵染效应及Olsen-P含量具有显著影响,且接种G.m处理H_2O-Po、NaHCO_3-P、NaOH-P较不接种处理(N.i)差异显著,4个处理对H_2O-Pi无显著差异,而HCl-P与残渣磷不接种处理(N.i)较接种G.m处理差异显著。
【Objective】The present paper aimed to screen out high quality AM fungi and improve soil fertility and phosphorus availability in the mining area.【Method】Through the pot experiment, the inoculation of Glomus intraradices, Glomus mosseae, Glomus etunicatum and No inoculation in the reclaimed soil of Jinshan coal mine in Jiexiu city, Shanxi province were studied, respectively, which affected the content of Olsen-P and the phosphorus form of Hedley in abandoned soil of mining area.【Result】The G.m inoculation significantly increased root Arbuscule richness, infection rate and infection density and promoted the formation and growth of mycelium and spore structure. At the same time, G.m inoculation significantly increased soil Olsen-P, H_2O-P, NaHCO_3-P, NaOH-P content; G.m showed that the inoculation promoted the formation of different forms of phosphorus in soil and improve the soil nutrient activation. The soil phosphorus utilization rate of phosphorus residue, HCl-P and stable structure, with no inoculation of AMF inoculation had no significant difference. The combination of effective phosphorus in soil and mycorrhizal infection and Hedley phosphorus components in phosphorus content obtained in the coal mine waste soil, the inoculation with arbuscular mycorrhizal fungi(arbuscular mycorrhizal, fungus, AMF for each group) had maize infection effect and Hedley phosphorus content in the different contributions, and the inoculation of G.m in the best contribution.【Conclusion】As for Corn infection effect and Olsen-P content, inoculation Glomus mosseae has a significant impact, inoculation Glomus mosseae for H_2O-Po, NaHCO_3-P,NaOH-P have a significant difference, 4 treatments have no significant difference to H_2O-Pi,the treatment of HCl-P and residue phosphorus without inoculation(N.i) was significantly different from that of inoculated G.m.
【Objective】The present paper aimed to screen out high quality AM fungi and improve soil fertility and phosphorus availability in the mining area.【Method】Through the pot experiment, the inoculation of Glomus intraradices, Glomus mosseae, Glomus etunicatum and No inoculation in the reclaimed soil of Jinshan coal mine in Jiexiu city, Shanxi province were studied, respectively, which affected the content of Olsen-P and the phosphorus form of Hedley in abandoned soil of mining area.【Result】The G.m inoculation significantly increased root Arbuscule richness, infection rate and infection density and promoted the formation and growth of mycelium and spore structure. At the same time, G.m inoculation significantly increased soil Olsen-P, H_2O-P, NaHCO_3-P, NaOH-P content; G.m showed that the inoculation promoted the formation of different forms of phosphorus in soil and improve the soil nutrient activation. The soil phosphorus utilization rate of phosphorus residue, HCl-P and stable structure, with no inoculation of AMF inoculation had no significant difference. The combination of effective phosphorus in soil and mycorrhizal infection and Hedley phosphorus components in phosphorus content obtained in the coal mine waste soil, the inoculation with arbuscular mycorrhizal fungi(arbuscular mycorrhizal, fungus, AMF for each group) had maize infection effect and Hedley phosphorus content in the different contributions, and the inoculation of G.m in the best contribution.【Conclusion】As for Corn infection effect and Olsen-P content, inoculation Glomus mosseae has a significant impact, inoculation Glomus mosseae for H_2O-Po, NaHCO_3-P,NaOH-P have a significant difference, 4 treatments have no significant difference to H_2O-Pi,the treatment of HCl-P and residue phosphorus without inoculation(N.i) was significantly different from that of inoculated G.m.
引文
[1]赵陟峰,郭建斌,郭汉清,等. 山西葛铺煤矿矿区土壤水分和结构变化浅析[J]. 水土保持研究,2008,15(3):214-216.
[2]马晓艳,田宇,王寅冬. 煤矿地下开采引发地面塌陷的危险性评估[J]. 山西建筑,36(12):120-121.
[3]刘惠欣, 李富平. 矿区尾矿土壤生物修复技术[J]. 江苏环境科技,2008,21(1):139-141.
[4]刘世亮,骆永明,丁克强,等. 菌根真菌对土壤中有机污染物的修复研究[J]. 地球科学进展,2004,19(2):198-203.
[5]Hirrel M C, Gerdemano J W. Improved growth of onion and dell pepper in saline soilsby two vesicular-arbuscularmycorrhizal fungi[J]. Plant and Soil, 1980, 44: 654-655.
[6]Gardner W K, Barber D A. The acquisition of phosphorusLupinus albusL. Il.l The probablemechanism bywhich phosphorus movement in the soil/root interface is enhanced[J]. Plant and Soil, 1983, 70: 107-124.
[7]Ae N J, Arlhara K,Okada, et al.Phosphorusuptake by pigeon pea and its role in cropping systems of Indian subcontonent[J].Science, 1999, 248: 477-480.
[8]Foshe D N, Classen, Jungk A. Phosphorus efficiency of plant, II Significance of root radius,Roothairs and cation-anion balance for phosphorus in flux in severplant species[J]. Plant and Soil, 2003, 132: 261-272.
[9]Eissenstat D M. Costs and benefits of constructing roots of small diameter[J].Plant Nutrition, 1992, 15: 763-782.
[10]Poss J A, Pond E, Menge J A, et al. Effect of salinity on my corrhizal on ion and tomato in soil with and without additional phosphate[J]. Plant and Soil, 2002, 88: 307-319.
[11]Bowen G. The biology and the physiology of my corrhiza and it's development[J].New Phytologist, 2001, 84: 27-57.
[12]李淑敏,李隆,张福锁.蚕豆/玉米间作接种 AM 真菌与根瘤菌对其吸磷量的影响[J].中国生态农业学报,2005,13(3):136 -139.
[13]Estaun M V. Effect of sodium chloride and mannitolon germination and hyphal growth of vesicular-arbuscular mycorrhizal fungus Glomusmosseae[J]. Agriculture Ecosystems and Environment, 1989, 29: 123-129.
[14]Hayman D S,Mosse B. Plant growth responses to vesicular-arbuscular mycorrhiza[J].New Phytologist, 1972, 71: 41-47.
[15]Ratnayake R T, Leonard R T, Menge J A. Root exudation to supply Of phosphorus and its possible relevance to mycorrhizal formation[J]. New Phytologist, 1998, 81: 543-552.
[16]杨振寅,廖声熙. 丛枝菌根对植物抗性的影响研究进展[J]. 世界林业研究,2005,18(2):26-29.
[17]王发园,林先贵. 丛枝菌根在植物修复重金属污染土壤中的作用[J].生态学报,2007,27(2):793-801.
[18]杜善周,毕银丽,吴王燕,等. 丛枝菌根对矿区环境修复的生态效应[J]. 农业工程学报,2008,24(4):113-116.
[19]陈芬,洪坚平,郝鲜俊,等. 不同培肥处理对采煤塌陷地复垦土壤Hedley P形态的影响[J]. 山西农业科学,2012,40(3):243-245.
[20]CHANG C S,JACKSON L M.Fractionation of soil phosphorus[J].Soil Science, 1957, 84(2): 133-144.
[21]Anon.An evaluation of nh4f as a selective extractant for aluminium phosphate in two soils of the tropics-sciencedirect[J]. s.n..
[22]Anon.Balancing development of major coal bases with available water resources in china through 2020-sciencedirect[J]. s.n..
[23]Eric T. Levy, William H. Schlesinger. A comparison of fractionation methods for forms of phosphorus in soils[J].Biogeochemistry, 1999, 47(1): 25-38.
[24]Eric T. Levy,William H. Schlesinger. A comparison of fractionation methods for forms of phosphorus in soils[M].S.l.:Kluwer Academic Publishers, 1999: 25-38.
[25]COBB A J. How firms shape income inequality: stakeholder power, executive decision making, and the structuring of employment relationships[J]. Acad Manage Rev, 2016, 41(2): 324-348.
[26]向万胜,黄敏,李学垣.土壤磷素的化学组分及其植物有效性[J]. 植物营养与肥料学报,2004,10(6): 663-670.
[27]杜佳佳.土壤磷素分级及植物有效性研究[D]. 南京:南京林业大学,2008: 48-56.
[28]吕家珑,张一平,陶国树,等.23年肥料定位实验0～100 cm土壤剖面中各形态磷之间的关系研究[J].水土保持学报,2003,17(3):48-50.
[29]穆晓慧,李世清,党蕊娟. 黄土高原石灰性土壤不同形态磷组分分布特征[J]. 中国生态农业学报,2008(6):1341-1347.
[30]鲁如坤,时正元,钱乘梁. 土壤积累态磷研究.III几种典型土壤中累积态磷的形态特征及其有效性[J]. 土壤,1997,29(2):57-60.
[31]吴玉梅. 长期定位施肥对蔬菜保护地土壤磷素形态的影响[J]. 中国土壤与肥料,2009(3):39-43.
[32]秦胜金,刘景双,王国平,等. 三江平原湿地土壤磷形态转化动态[J]. 生态学报,2007,27(9):3844-3851.
[33]SuiY B,Thompson M L,Shang C. Fractionation of phosphorus in amollisol amended with biosolids[J]. Soil Sci.Am,1999,63:1174-1180.
[34]杨芳,何园球,李成亮,等. 不同施肥条件下红壤旱地磷素形态及有效性分析[J]. 土壤学报,2006,43(5):793-799.
[35]谢英荷,洪坚平,韩旭,等. 不同磷水平石灰性土壤Hedley磷形态生物有效性的研究[J]. 水土保持学报,2010,24(6):141-144.
[36]ZHANG T Q,MACKENZIE A F,LIANG B C,et al.Soil test phosphorus and phosphorus fractions with long-term phosphorus addition and depletion[J].Soil Science Society of America Journal, 2004, 68(2): 519-528.
[37]张奇春,王光火,冯玉科. 水稻肥料定位试验中土壤各形态磷的变化动态研究[J]. 浙江大学学报(农业与生命科学版),2007(1):82-88.
[2]马晓艳,田宇,王寅冬. 煤矿地下开采引发地面塌陷的危险性评估[J]. 山西建筑,36(12):120-121.
[3]刘惠欣, 李富平. 矿区尾矿土壤生物修复技术[J]. 江苏环境科技,2008,21(1):139-141.
[4]刘世亮,骆永明,丁克强,等. 菌根真菌对土壤中有机污染物的修复研究[J]. 地球科学进展,2004,19(2):198-203.
[5]Hirrel M C, Gerdemano J W. Improved growth of onion and dell pepper in saline soilsby two vesicular-arbuscularmycorrhizal fungi[J]. Plant and Soil, 1980, 44: 654-655.
[6]Gardner W K, Barber D A. The acquisition of phosphorusLupinus albusL. Il.l The probablemechanism bywhich phosphorus movement in the soil/root interface is enhanced[J]. Plant and Soil, 1983, 70: 107-124.
[7]Ae N J, Arlhara K,Okada, et al.Phosphorusuptake by pigeon pea and its role in cropping systems of Indian subcontonent[J].Science, 1999, 248: 477-480.
[8]Foshe D N, Classen, Jungk A. Phosphorus efficiency of plant, II Significance of root radius,Roothairs and cation-anion balance for phosphorus in flux in severplant species[J]. Plant and Soil, 2003, 132: 261-272.
[9]Eissenstat D M. Costs and benefits of constructing roots of small diameter[J].Plant Nutrition, 1992, 15: 763-782.
[10]Poss J A, Pond E, Menge J A, et al. Effect of salinity on my corrhizal on ion and tomato in soil with and without additional phosphate[J]. Plant and Soil, 2002, 88: 307-319.
[11]Bowen G. The biology and the physiology of my corrhiza and it's development[J].New Phytologist, 2001, 84: 27-57.
[12]李淑敏,李隆,张福锁.蚕豆/玉米间作接种 AM 真菌与根瘤菌对其吸磷量的影响[J].中国生态农业学报,2005,13(3):136 -139.
[13]Estaun M V. Effect of sodium chloride and mannitolon germination and hyphal growth of vesicular-arbuscular mycorrhizal fungus Glomusmosseae[J]. Agriculture Ecosystems and Environment, 1989, 29: 123-129.
[14]Hayman D S,Mosse B. Plant growth responses to vesicular-arbuscular mycorrhiza[J].New Phytologist, 1972, 71: 41-47.
[15]Ratnayake R T, Leonard R T, Menge J A. Root exudation to supply Of phosphorus and its possible relevance to mycorrhizal formation[J]. New Phytologist, 1998, 81: 543-552.
[16]杨振寅,廖声熙. 丛枝菌根对植物抗性的影响研究进展[J]. 世界林业研究,2005,18(2):26-29.
[17]王发园,林先贵. 丛枝菌根在植物修复重金属污染土壤中的作用[J].生态学报,2007,27(2):793-801.
[18]杜善周,毕银丽,吴王燕,等. 丛枝菌根对矿区环境修复的生态效应[J]. 农业工程学报,2008,24(4):113-116.
[19]陈芬,洪坚平,郝鲜俊,等. 不同培肥处理对采煤塌陷地复垦土壤Hedley P形态的影响[J]. 山西农业科学,2012,40(3):243-245.
[20]CHANG C S,JACKSON L M.Fractionation of soil phosphorus[J].Soil Science, 1957, 84(2): 133-144.
[21]Anon.An evaluation of nh4f as a selective extractant for aluminium phosphate in two soils of the tropics-sciencedirect[J]. s.n..
[22]Anon.Balancing development of major coal bases with available water resources in china through 2020-sciencedirect[J]. s.n..
[23]Eric T. Levy, William H. Schlesinger. A comparison of fractionation methods for forms of phosphorus in soils[J].Biogeochemistry, 1999, 47(1): 25-38.
[24]Eric T. Levy,William H. Schlesinger. A comparison of fractionation methods for forms of phosphorus in soils[M].S.l.:Kluwer Academic Publishers, 1999: 25-38.
[25]COBB A J. How firms shape income inequality: stakeholder power, executive decision making, and the structuring of employment relationships[J]. Acad Manage Rev, 2016, 41(2): 324-348.
[26]向万胜,黄敏,李学垣.土壤磷素的化学组分及其植物有效性[J]. 植物营养与肥料学报,2004,10(6): 663-670.
[27]杜佳佳.土壤磷素分级及植物有效性研究[D]. 南京:南京林业大学,2008: 48-56.
[28]吕家珑,张一平,陶国树,等.23年肥料定位实验0～100 cm土壤剖面中各形态磷之间的关系研究[J].水土保持学报,2003,17(3):48-50.
[29]穆晓慧,李世清,党蕊娟. 黄土高原石灰性土壤不同形态磷组分分布特征[J]. 中国生态农业学报,2008(6):1341-1347.
[30]鲁如坤,时正元,钱乘梁. 土壤积累态磷研究.III几种典型土壤中累积态磷的形态特征及其有效性[J]. 土壤,1997,29(2):57-60.
[31]吴玉梅. 长期定位施肥对蔬菜保护地土壤磷素形态的影响[J]. 中国土壤与肥料,2009(3):39-43.
[32]秦胜金,刘景双,王国平,等. 三江平原湿地土壤磷形态转化动态[J]. 生态学报,2007,27(9):3844-3851.
[33]SuiY B,Thompson M L,Shang C. Fractionation of phosphorus in amollisol amended with biosolids[J]. Soil Sci.Am,1999,63:1174-1180.
[34]杨芳,何园球,李成亮,等. 不同施肥条件下红壤旱地磷素形态及有效性分析[J]. 土壤学报,2006,43(5):793-799.
[35]谢英荷,洪坚平,韩旭,等. 不同磷水平石灰性土壤Hedley磷形态生物有效性的研究[J]. 水土保持学报,2010,24(6):141-144.
[36]ZHANG T Q,MACKENZIE A F,LIANG B C,et al.Soil test phosphorus and phosphorus fractions with long-term phosphorus addition and depletion[J].Soil Science Society of America Journal, 2004, 68(2): 519-528.
[37]张奇春,王光火,冯玉科. 水稻肥料定位试验中土壤各形态磷的变化动态研究[J]. 浙江大学学报(农业与生命科学版),2007(1):82-88.