蛋白质组学在细胞信号通路研究中的应用
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摘要
细胞中各种信号转导与生物学过程密切相关,而蛋白质在信号传导过程中起着至关重要的作用。蛋白质组学从整体水平上研究蛋白质组,可系统地研究生物体生理生化以及与疾病发生发展相关的功能性蛋白的表达,是研究细胞信号通路的有效方法之一。目前,蛋白质组学技术已经应用于各种信号通路研究中,并取得了诸多进展。本文综述了蛋白质组学在肝脏疾病、肿瘤、病原微生物致病机制以及机体代谢相关通路的研究等方面的应用,以期为蛋白质组学在相关领域的进一步应用研究提供参考。
Various signal transduction pathways in cells are closely related to the biological processes,while the proteins play an important role in the process of signal transductions. Proteomics, which is one of the effective methods for the study of cell signal pathways,can conduct proteomic analysis systematically as well as explore the expression of functional proteins related to the physiological characteristics in organism and in the initiation and progression of diseases. Nowadays,proteomics has been successfully applied in the studies of many kinds of signal pathways. In this paper,proteomic study in signal pathways related to liver disease,tumors,pathogenic mechanism of pathogens and metabolism are reviewed,in order to provide a reference for future research and applications of proteomics in the related fields.
Various signal transduction pathways in cells are closely related to the biological processes,while the proteins play an important role in the process of signal transductions. Proteomics, which is one of the effective methods for the study of cell signal pathways,can conduct proteomic analysis systematically as well as explore the expression of functional proteins related to the physiological characteristics in organism and in the initiation and progression of diseases. Nowadays,proteomics has been successfully applied in the studies of many kinds of signal pathways. In this paper,proteomic study in signal pathways related to liver disease,tumors,pathogenic mechanism of pathogens and metabolism are reviewed,in order to provide a reference for future research and applications of proteomics in the related fields.
引文
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[3]Yang XX,Wang K,Chen XJ,et al.Function and regulation of MAVS,the mitochondrial antiviral signaling protein in innate immunity[J].Prog Biochem Biophys(生物化学与生物物理进展),2013,40(5):397-405.
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[8]Farber GK.New approaches to rational drug design[J].Pharm Ther,1999,84(3):327-332.
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[10]Sohn J,Do KA,Liu S,et al.Functional proteomics characterization of residual triple-negative breast cancer after standard neoadjuvant chemotherapy[J].Ann Oncol,2013,24(10):2522-2526.
[11]Guo CY,Zhan KH.Research progress and application in technology of proteome[J].J Yunnan Agric Univ(云南农业大学学报),2010,25(4):583-591.
[12]Chen Y,Xu Y,Ying HJ,et al.Research progress of affinity chromatography[J].Ion Exc Adsorp(离子交换与吸附),2001,17(3):276-280.
[13]Zhen Y,Shi JS.Application of mass spectrometry in proteomics studies[J].J Nanjing Forestry Univ(Nat Sci)(南京林业大学学报),2011,35(1):103-108.
[14]Nesvizhskii AI,Vitek O,Aebersold R.Analysis and validation of proteomic data generated by tandem mass spectrometry[J].Nat Methods,2007,4(10):787-797.
[15]Deng JS,Zhao F,Yu XY,et al.Identification of the protective role of DJ-1 in hypoglycemicastrocyte injury using proteomics[J].J Proteome Res,2015,14:2839-2848.
[16]Cheng TL,Chen JH,Wang PK,et al.Quantitative proteomics analysis reveals that S-nitrosoglutathione reductase(GSNOR)and nitric oxide signaling enhance poplar defense against chilling stress[J].Planta,2015,242:1361-1390.
[17]Mok L,Wynne JW,Ford K,et al.Proteomic analysis of pteropusalecto kidney cells in response to the viral mimic,Poly I:C[J].Proteome Sci,2015,13(25):2-11.
[18]Xia QC,Zeng R,Cao XJ,et al.Protein Chemistry and Proteomics(蛋白质化学与蛋白质组学)[M].Beijing:Science Publishing Company,2004:467-470.
[19]Shan Q,Lou XM,Xiao T,et al.Cancer/testis antigen microarray to screen autoantibody biomarkers of non-small cell lung cancer[J].Cancer Lett,2013,328(1):160-167.
[20]Xiao XY,Wei XP,He DC.Applicationof protein microarraytechnique in screening of serum lung cancer marker proteins[J].Chin Sci(Series C)[中国科学(C辑)],2003,33(4):323-328.
[21]Fields S,Song OK.A novel genetic system to detect protein-protein interactions[J].Nature,1989,340(6230):245-246.
[22]Liu Z,Shi L,Tang Q,et al.Genome-wide identification and transcriptional expression analysis of mitogen-activated protein kinase and mitogen-activated protein kinase kinase genes in Capsicum annuum[J].Frout Plant Sci,2015,6:780.
[23]Park SY,Choi HK,Seo JS,et al.DNAJB1 negatively regulates MIG6 to promote epidermal growth factor receptor signaling[J].Biochim Biophy Acta,2015,1853(10):2722-2730.
[24]Hadweh P,Habelhah H,Kieff E,et al.The PP4R1 subunit of protein phosphatase PP4 targets TRAF2 and TRAF6 to mediate inhibition of NF-κB activation[J].Cell Sig,2014,26(12):2730-2737.
[25]Wang XW,Guo TY,Peng F,et al.Proteomic and functional profiles of a follicle-stimulating hormone positive human nonfunctional pituitary adenoma[J].Electrophoresis,2015,36(11/12):1289-1304.
[26]Wu Z,Wang L,Guo ZG,et al.Experimental study on differences in clivuschordoma bone invasion:an i TRAQ-based quantitative proteomic analysis[J].PLo S ONE,2015,10(3):e011952.
[27]Peng XC,Gong FM,Chen Y,et al.Proteomics identification of PGAM1 as a potential therapeutic target for urothelial bladder cancer[J].J Proteomics,2016,132:85-92.
[28]Li LD,Sun HF,Liu XX,et al.Down-regulation of NDUFB9 promotes breast cancer cell proliferation,metastasis by mediating mitochondrial metabolism[J].PLo S ONE,2015,10(12):e0144441.
[29]Zanivan S,Meves A,Behrendt K,et al.In vivo SILAC-based proteomics reveals phosphoproteome changes during mouse skin carcinogenesis[J].Cell Rep,2013,3(2):552-566.
[30]Ying Q,Ansong E,Diamond AM,et al.Quantitative proteomic analysis reveals that anti-cancer effects of selenium-binding protein 1 in vivo are associated with metabolic pathways[J].PLo S ONE,2015,10(5):e0126285.
[31]Lin YY,Xie GB,Xia J,et al.TBMS1 exerts its cytotoxicity in NCI-H460 lung cancer cells through nucleolar stress-induced p53/MDM2-dependent mechanism,a quantitative proteomics study[J].Biochim Biophy Acta,2016,1864(2):204-210.
[32]Ger M,Kaupinis A,Ceniene AN,et al.Quantitative proteomic analysis of anticancer drug RH1 resistance inliver carcinoma[J].BBA-Proteins Proteom,2016(1864):219-232.
[33]Diamond DL,Proll SC,Jacobs JM,et al.Proteomics:applying proteomic technologies to the study of liver function and disease[J].Hepatology,2006,44(2):299-308.
[34]Vildhede A,Wis'n iewski JR,Norén A,et al.Comparative proteomic analysis of human liver tissue and isolated hepatocytes with a focus on proteins determining drug exposure[J].J Proteome Res,2015,14(8):3305-3314.
[35]Liu Y,Dai M,Bi Y,et al.Active smoking,passive smoking,and risk of nonalcoholic fatty disease(NAFLD):a population-based study in china[J].J Epidemiol,2013,23(2):115-121.
[36]Calvert VS,Collantes R,Lilariny H,et al.A systems biology approach to the pathogenesis of obesity-related nonalcoholic fatty liver disease using reverse phase protein microarrays for multiplexed cell signaling analysis[J].Hepatology,2007,46(1):166-172.
[37]Thomas A,Stevens AP,Klein MS,et al.Early changes in the liver-soluble proteome from mice fed a nonalcoholic steatohepatitis inducing diet[J].Proteomics,2012,12(9):1437-1451.
[38]Murakami Y,Yasuda T,Saigo K,et al.Comprehensive analysis of microRNA expression patterns in hepatocellular carcinoma and non-tumorous tissues[J].Oncogene,2006,25(17):2537-2545.
[39]Yang F,Yin YX,Wang F,et al.miR-17-5p promotes migration of human hepatocellular carcinoma cells through the p38 mitogenactivated protein kinase-heat shock protein 27 pathway[J].Hepatology,2010,51(5):1614-1623.
[40]Xu L,Chen S,Bergan RC.MAPKAPK2 and HSP27 are downstream effectors of p38 MAP kinase-mediated matrix metalloproteinase type 2 activation and cell invasion in human prostate cancer[J].Oncogene,2006,25(21):2987-2998.
[41]Wang SH,Huang X,Lao HM,et al.RN181 suppresses hepatocellular carcinoma growth byinhibition of the ERK/MAPK pathway[J].Hepatology,2011,53(6):1932-1942.
[42]Borlak J,Singh P,Gazzana G.Proteome mapping of epidermal growth factor induced hepatocellular carcinomas identifies novel cell metabolism targets and mitogen activated protein kinase signaling events[J].BMC Genomics,2015,16(1):1312-1341.
[43]Xing XH,Huang Y,Wang S,et al.Comparative analysis of primary hepatocellular carcinoma with single and multiple lesions by i TRAQ-based quantitative proteomics[J].J Proteomics,2015,128:262-271.
[44]Lovet JM,Bruix J.Molecular targeted therapies in hepatocellular carcinoma[J].Hepatology,2008,48(4):1312-1327.
[45]Li YH,Tai CSW,Xue JY,et al.Proteomic study of pyrrolizidine alkaloid-Induced hepatic sinusoidal obstruction syndrome in rats[J].Chem Res Toxicol,2015,28(9):1715-1727.
[46]Liu Na,Yang HL,Wang P,et al.Functional proteomic analysis revels that thee thanol extract of Annona muricata L.induces liver cancer cell apoptosis through endoplasmic reticulum stress pathway[J].J Ethnopharmacol,2016,189:210-217.
[47]Ma ZJ,Wang XX,Su G,et al.Proteomic analysis of apoptosis induction by lariciresinol in human Hep G2 cells[J].Chem Biol Interact,2016,256:209-219.
[48]Guo ZG,Liu XJ,Li ML,et al.Differential urinary glycoproteome analysis of type 2 diabetic nephropathy using 2D-LC-MS/MS and i TRAQ quantification[J].J Transl Med,2015,13(1):371.
[49]Arrabal S,Lucena MA,Canduelaet MJ,et al.Pharmacological blockade of cannabinoid CB1 receptors in diet-induced obesity regulates mitochondrial dihydrolipoamide dehydrogenase in muscle[J].PLo S ONE,2015,10(12):e0145244.
[50]Chiu CW,Chen HM,Wu TT,et al.Differential proteomics of monosodium urate crystalsinduced inflammatory response in dissected murine air pouch membranes by i TRAQ technology[J].Proteomics,2015,15(19):3338-3348.
[51]Ahmad S,Kumar KA,Basak T,et al.PPAR signaling pathway is a key modulator of liver proteome in pups born to vitamin B12deficient rats[J].Protemics,2013,91:297-308.
[52]Ferre P.The biology of peroxisome proliferator-activated receptors:relationship with lipid metabolism and insulin sensitivity[J].Diabetes,2004,53(suppl 1):S43-50.
[53]Miller I,Serchi T,Cambier S,et al.Hexabromocyclododecane(HBCD)induced changes in the liver proteome of eu-and hypothyroid female rats[J].Toxicol Lett,2016,245:40-51.
[54]Ilavenil S,Al-Dhabi NA,Srigopalram S,et al.Acetaminophen induced hepatotoxicity in Wistar rats—A proteomic approach[J].Mol,2016,21(2):161.
[55]Kim SW,Park TJ,Chaudhari HN,et al.Hepatic proteome and its network response to supplementation of an anti-obesity herbal mixture in diet-induced obese mice[J].Biotechnol Bioproce Eng,2015,20(4):775-793.
[56]Liu Q,Zhao XP,Lu XY,et al.Proteomic study on usnic-acidinduced hepatotoxicity in rats[J].J Agric Food Chem,2012,60:7312-7317.
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