甲状腺癌超声造影参数与病灶内增殖、侵袭、自噬基因表达的相关性

详细信息    查看全文 | 推荐本文 |

英文篇名：Correlation of contrast-enhanced ultrasonography parameters of thyroid cancer with the expression of proliferation,invasion and autophagy genes in the lesions 作者：蒙秋凤 英文作者：MENG Qiu-feng;B-Ultrasound Room,the Second People's Hospital of Guigang in Guangxi Province; 关键词：甲状腺结节 ; 超声造影 ; 增殖 ; 侵袭 ; 自噬 英文关键词：Thyroid nodule;;Contrast-enhanced ultrasonography;;Proliferation;;invasion;;Autophagy 中文刊名：HNYY 英文刊名：Journal of Hainan Medical University 机构：广西贵港市第二人民医院B超室; 出版日期：2018-07-02 18:22 出版单位：海南医学院学报 年：2018 期：v.24;No.214 基金：贵港市科学技术项目(2016-J-3-13-01)~~ 语种：中文; 页：HNYY201815020 页数：4 CN：15 ISSN：46-1049/R 分类号：82-85

摘要

目的:探讨甲状腺癌超声造影参数与病灶内增殖、侵袭、自噬基因表达的相关性。方法:收集2014年12月～2017年1月在本院接受甲状腺结节切除术的患者218例,根据病理结果分为甲状腺腺瘤组129例、甲状腺癌组89例。回顾其术前甲状腺结节超声造影参数水平,对比两组肿瘤组织中增殖、侵袭、自噬基因的表达量。采用Pearson检验评估甲状腺癌性结节超声造影参数水平与癌细胞恶性生物学行为的相关关系。结果:甲状腺癌组患者PI、TTP、MTT的水平低于甲状腺腺瘤组患者;甲状腺癌组肿瘤组织中PDCD4、PP4R1mRNA的表达量低于甲状腺腺瘤组,TPX2、Twist1mRNA的表达量高于甲状腺腺瘤组;甲状腺癌组肿瘤组织中ADAM9、BCORL1、CXCR4 mRNA的表达量高于甲状腺腺瘤组,Notch-1、ST7L mRNA的表达量低于甲状腺腺瘤组;甲状腺癌组肿瘤组织中Beclin1、LC3、PTEN mRNA的表达量均显著低于甲状腺腺瘤组。Pearson检验发现,甲状腺癌超声造影参数与增殖、侵袭、自噬基因的表达量直接相关。结论:超声造影可用于鉴别甲状腺良恶性结节,且具体造影参数水平可反映肿瘤恶性程度。
        Objective:To explore the correlation of contrast-enhanced ultrasonography parameters of thyroid cancer with the expression of proliferation,invasion and autophagy genes in the lesions.Methods:A total of 218 patients who underwent thyroid nodule resection in our hospital between December 2014 and January 2017 were collected and divided into the thyroid adenoma group(n=129)and the thyroid cancer group(n=89)according to the pathological results.The preoperative CEUS parameter levels of thyroid cancer were reviewed,and the expression levels of proliferation,invasion and autophagy genes in tumor tissues were compared between the two groups.Pearson test was used to evaluate the correlation between the CEUS parameter levels of cancerous thyroid nodule and the malignant biological behaviors of cancer cells.Results:PI,TTP and MTT levels in thyroid cancer group were lower than those in thyroid adenoma group;PDCD4 and PP4 R1 mRNA expression in tumor tissues of thyroid cancer group were lower than those of thyroid adenoma group whereas TPX2 and Twist1 mRNA expression were higher than those of thyroid adenoma group;ADAM9,BCORL1 and CXCR4 mRNA expression in tumor tissues of thyroid cancer group were higher than those of thyroid adenoma group whereas Notch-1 and ST7 L mRNA expression were lower than those of thyroid adenoma group;Beclin1,LC3 and PTEN mRNA expression in tumor tissues of thyroid cancer group were significantly lower than those of thyroid adenoma group(P<0.05).Pearson test showed that the contrast-enhanced ultrasonography parameters of thyroid cancer were directly correlated with the expression of proliferation,invasion and autophagy genes.Conclusion:Contrast-enhanced ultrasonography can be used to identify benign and malignant thyroid nodules,and the specific CEUS parameter levels can reflect the tumor malignancy.

引文

1 Voss RK,Grubbs EG.Response to letter:"medullary thyroid carcinoma in MEN2A:ATA moderate-or high-risk ret mutations do not predict disease aggressiveness"[J].J Clin Endocrinol Metab,2017,102(9):3559.
    2 Cote GJ,Evers C,Hu MI,et al.Prognostic significance of circulating RET M918T mutated tumor DNA in patients with advanced medullary thyroid carcinoma[J].J Clin Endocrinol Metab,2017,102(9):3591-3599.
    3 Li F,Wang Y,Bai B,et al.Advantages of routine ultrasound combined with contrast-enhanced ultrasound in diagnosing papillary thyroid carcinoma[J].Ultrasound Q,2017,33(3):213-218.
    4 Liu Y,Liu H,Qian CL,et al.Utility of quantitative contrastenhanced ultrasound for the prediction of extracapsular extension in papillary thyroid carcinoma[J].Sci Rep,2017,7(1):1472.
    5 Yuan Z,Quan J,Yunxiao Z,et al.Association between realtime contrast-enhanced ultrasound characteristics and thyroid carcinoma size[J].Mol Clin Oncol,2015,3(4):743-746.
    6 Müller-Peltzer K,Rübenthaler J,Reiser M,et al.Contrast-enhanced ultrasound(CEUS)of the liver:Critical evaluation of use in clinical routine diagnostics[J].Radiologe,2017,57(5):348-355.
    7 Zhang YZ,Xu T,Gong HY,et al.Application of high-resolution ultrasound,real-time elastography,and contrast-enhanced ultrasound in differentiating solid thyroid nodules[J].Medicine(Baltimore),2016,95(45):e5329.
    8 Wiesinger I,Kroiss E,Zausig N,et al.Analysis of arterial dynamic micro-vascularization with contrast-enhanced ultrasound(CEUS)in thyroid lesions using external perfusion software:First results[J].Clin Hemorheol Microcirc,2016,64(4):747-755.
    9 Zhang Y,Zhou P,Tian SM,et al.Usefulness of combined use of contrast-enhanced ultrasound and TI-RADS classification for the differentiation of benign from malignant lesions of thyroid nodules[J].Eur Radiol,2017,27(4):1527-1536.
    10 Wu Q,Wang Y,Li Y,et al.Diagnostic value of contrast-enhanced ultrasound in solid thyroid nodules with and without enhancement[J].Endocrine,2016,53(2):480-488.
    11 Pennelli G,Galuppini F,Barollo S,et al.The PDCD4/miR-21pathway in medullary thyroid carcinoma[J].Hum Pathol,2015,46(1):50-57.
    12 Yang X,Liu G,Xiao H,et al.TPX2overexpression in medullary thyroid carcinoma mediates TT cell proliferation[J].Pathol Oncol Res[J].2014,20(3):641-648.
    13 Lv N,Shan Z,Gao Y,et al.Twist1regulates the epithelialmesenchymal transition via the NF-κB pathway in papillary thyroid carcinoma[J].Endocrine,2016,51(3):469-477.
    14 Mato E,Gonzlez C,Moral A,et al.ABCG2/BCRP gene expression is related to epithelial-mesenchymal transition inducer genes in a papillary thyroid carcinoma cell line(TPC-1)[J].JMol Endocrinol,2014,52(3):289-300.
    15 Fu H,Ma C,Guan W,et al.Expression of Notch 1receptor associated with tumor aggressiveness in papillary thyroid carcinoma[J].Onco Targets Ther,2016,9(1):1519-1523.
    16 Zhang H,Teng X,Liu Z,et al.Gene expression profile analyze the molecular mechanism of CXCR7regulating papillary thyroid carcinoma growth and metastasis[J].J Exp Clin Cancer Res,2015,12(34):16.
    17 Smallridge RC,Chindris AM,Asmann YW,et al.RNA sequencing identifies multiple fusion transcripts,differentially expressed genes,and reduced expression of immune function genes in BRAF(V600E)mutant vs BRAF wild-type papillary thyroid carcinoma[J].J Clin Endocrinol Metab,2014,99(2):E338-E347.
    18 Yang M,Bai L,Yu W,et al.Expression of autophagy-associated proteins in papillary thyroid carcinoma[J].Oncol Lett,2017,14(1):411-415.
    19 Zhang L,Cheng X,Gao Y,et al.Apigenin induces autophagic cell death in human papillary thyroid carcinoma BCPAP cells[J].Food Funct,2015,6(11):3464-3472.
    20 Sadow PM,Dias-Santagata D,Zheng Z,et al.Identification of insertions in PTEN and TP53in anaplastic thyroid carcinoma with angiogenic brain metastasis[J].Endocr Relat Cancer,2015,22(6):L23-L28.
    21 Beg S,Siraj AK,Jehan Z,et al.PTEN loss is associated with follicular variant of Middle Eastern papillary thyroid carcinoma[J].Br J Cancer,2015,112(12):1938-1943.