NUSAP1在膀胱尿路上皮癌中的表达及对预后评估的价值
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摘要
目的:检测核仁和纺锤体相关蛋白1(NUSAP1)在膀胱尿路上皮癌中的表达及对预后评估的价值。方法:采用免疫组化方法检测NUSAP1在84例膀胱尿路上皮癌组织及10例正常膀胱上皮组织中的表达情况,并分析其表达与临床病理特征的关系;采用Western blot检测NUSAP1蛋白在T24和SV-HUC-1细胞系中的表达;采用单因素和多因素COX回归分析评价NUSAP1在膀胱尿路上皮癌中的预后价值。结果:免疫组织化学结果显示NUSAP1在膀胱尿路上皮癌中的阳性表达率为72. 62%,在正常膀胱上皮组织中的阳性表达率为10. 00%,差异有统计学意义(P <0. 05)。NUSAP1在膀胱尿路上皮癌中的高表达与患者性别、年龄、肿瘤数目均无关(P> 0. 05),而与肿瘤复发、病理分级、临床分期、转移有关(P <0. 05)。NUSAP1蛋白在T24细胞系中的表达明显高于SV-HUC-1细胞系,差异有统计学意义(P <0. 05)。NUSAP1表达、临床分期和转移均为膀胱尿路上皮癌的独立预测因素。此外,Kaplan-Meier曲线表明,NUSAP1阳性表达的膀胱尿路上皮癌患者无进展生存时间(PFS)明显少于NUSAP1阴性表达者。结论:NUSAP1在膀胱尿路上皮癌中呈现高表达,且与肿瘤复发、病理分级、临床分期、转移有关,NUSAP1是膀胱尿路上皮癌独立危险因素,其高表达提示预后较差,具有一定的临床价值。
Objective: To detect the expression and prognosis of nucleolar and spindle-associated protein 1( NUSAP1) in bladder urothelial carcinoma. Methods: The expression of NUSAP1 in 84 cases of bladder urothelial carcinoma and 10 cases of normal bladder epithelium were detected by immunohistochemistry,and the relationship between NUSAP1 expression and clinicopathological features was analyzed. Western blot method detected NUSAP1 protein expression on T24 and SV-HUC-1 cell line. The prognostic value of NUSAP1 in bladder urothelial carcinoma was evaluated by COX regression analysis. Results: The positive rate of NUSAP1 expression in bladder urothelial carcinoma was 7 2. 6 2 %,and that in normal bladder epithelium was 10. 00%. The difference was statistically significant( P < 0. 05). The high expression of NUSAP1 in bladder urothelial carcinoma was not correlated with gender,age and tumor number( P > 0. 05),but correlated with tumor recurrence,pathological grade,clinical stage and metastasis( P< 0. 05). The expression of NUSAP1 protein in T24 cell line was significantly higher than that in SV-HUC-1 cell line( P < 0. 05). NUSAP1 expression,clinical stage and metastasis were independent predictors of bladder urothelial carcinoma. In addition,Kaplan-Meier curves showed that progression-free survival( PFS) in NUSAP1-positive bladder urothelial carcinoma patients was significantly less than that in NUSAP1-negative bladder urothelial carcinoma patients. Conclusion: NUSAP1 is highly expressed in bladder urothelial carcinoma,and is related to tumor recurrence,pathological grade,clinical stage and metastasis. NUSAP1 is an independent risk factor for bladder urothelial carcinoma. Its high expression suggests poor prognosis and has certain clinical value.
Objective: To detect the expression and prognosis of nucleolar and spindle-associated protein 1( NUSAP1) in bladder urothelial carcinoma. Methods: The expression of NUSAP1 in 84 cases of bladder urothelial carcinoma and 10 cases of normal bladder epithelium were detected by immunohistochemistry,and the relationship between NUSAP1 expression and clinicopathological features was analyzed. Western blot method detected NUSAP1 protein expression on T24 and SV-HUC-1 cell line. The prognostic value of NUSAP1 in bladder urothelial carcinoma was evaluated by COX regression analysis. Results: The positive rate of NUSAP1 expression in bladder urothelial carcinoma was 7 2. 6 2 %,and that in normal bladder epithelium was 10. 00%. The difference was statistically significant( P < 0. 05). The high expression of NUSAP1 in bladder urothelial carcinoma was not correlated with gender,age and tumor number( P > 0. 05),but correlated with tumor recurrence,pathological grade,clinical stage and metastasis( P< 0. 05). The expression of NUSAP1 protein in T24 cell line was significantly higher than that in SV-HUC-1 cell line( P < 0. 05). NUSAP1 expression,clinical stage and metastasis were independent predictors of bladder urothelial carcinoma. In addition,Kaplan-Meier curves showed that progression-free survival( PFS) in NUSAP1-positive bladder urothelial carcinoma patients was significantly less than that in NUSAP1-negative bladder urothelial carcinoma patients. Conclusion: NUSAP1 is highly expressed in bladder urothelial carcinoma,and is related to tumor recurrence,pathological grade,clinical stage and metastasis. NUSAP1 is an independent risk factor for bladder urothelial carcinoma. Its high expression suggests poor prognosis and has certain clinical value.
引文
[1] Sun FH. Expression and significance of Rab25,E-cadherin and Twist in transitional cell carcinoma of bladder[D]. Shandong University,2015.[孙发海. Rab25、E-cadherin和Twist在膀胱移行细胞癌中的表达及意义[D].山东大学,2015.]
[2] Liu Z,Guan C,Lu C,et al. High NUSAP1 expression predicts poor prognosis in colon cancer[J]. Pathology-Research and Practice,2018,214(7):968-973.
[3] Kretschmer C,Sternerkock A,Siedentopf F,et al. Identification of early molecular markers for breast cancer[J]. Molecular Cancer,2011,10(1):15.
[4] Fang L,Zhang M,Chen L,et al. Downregulation of nucleolar and spindle-associated protein 1 expression suppresses cell migration,proliferation and invasion in renal cell carcinoma[J]. Oncology Reports,2016,36(3):1506.
[5] Han G,Wei Z,Cui H,et al. NUSAP1 gene silencing inhibits cell proliferation,migration and invasion through inhibiting DNMT1gene expression in human colorectal cancer[J]. Experimental Cell Research,2018,367(2):216-221.
[6] Gordon CA,Gong X,Ganesh D,et al. NUSAP1 promotes invasion and metastasis of prostate cancer[J]. Oncotarget,2017,8(18):29935-29950.
[7] Chen L,Yang L,Qiao F,et al. High levels of nucleolar spindleassociated protein and reduced levels of BRCA1 expression predict poor prognosis in triple-negative breast cancer[J]. PLo S One,2015,10(10):e0140572.
[8] Chen B,Li Y,Wei YY,et al. The expression and clinical significance of NUSAP1 in non-small cell lung cancer[J]. International Journal of Respiratory Medicine,2017,37(22):1681-1684.[陈波,李悦,位云艳,等.非小细胞肺癌中NUSAP1的表达及临床意义[J].国际呼吸杂志,2017,37(22):1681-1684.]
[9] Xue YP,Ji XY,Yang L,et al. Correlation between nucleolar spindle-associated proteins and malignant progression and prognosis of human glioblastoma[J]. Chinese Journal of Medicine,2018,98(5):340-345.[薛彦平,季晓燕,杨麟,等.核仁纺锤体相关蛋白与人脑胶质母细胞瘤恶性进展和预后的相关性分析[J].中华医学杂志,2018,98(5):340-345.]
[10] Hussain S,Sbnascimento B. The nucleolar RNA methyltransferase Misu(NSun2)is required for mitotic spindle stability[J]. Journal of Cell Biology,2009,186(1):27-40.
[11] Vanden BA,Raemaekers T,Denayer S,et al. Nu SAP is essential for chromatin-induced spindle formation during early embryogenesis[J]. Journal of Cell Science,2010,123(Pt 19):3244-3255.
[12] Gay O,Gilquin B,Nakamura F,et al. RefilinB(FAM101B)targets filamin A to organize perinuclear actin networks and regulates nuclear shape[J]. Proceedings of the National Academy of Sciences of the United States of America,2011,108(28):11464-11469.
[13] Nakazawa M,Kyprianou N. Epithelial-mesenchymal-transition regulators in prostate cancer:Androgens and beyond[J]. Journal of Steroid Biochemistry&Molecular Biology,2017,166:84-90.
[14] Zhang M,Yang DH,Liu X,et al. Expression of Nusap1 protein in hepatocellular carcinoma and its clinical significance[J]. Journal of Central South University(Medical Edition),2013,38(9):876-881.[张猛,杨定华,刘萧,等. Nusap1蛋白在肝癌中的表达及其临床意义[J].中南大学学报(医学版),2013,38(9):876-881.]
[15] Wu X,Xu B,Yang C,et al. Nucleolar and spindle associated protein 1 promotes the aggressiveness of astrocytoma by activating the Hedgehog signaling pathway[J]. Journal of Experimental&Clinical Cancer Research,2017,36(1):127.
[16] Okamoto A,Higo M,Shiiba M,et al. Down-regulation of nucleolar and spindle-associated protein 1(NUSAP1)expression suppresses tumor and cell proliferation and enhances anti-tumor effect of paclitaxel in oral squamous cell carcinoma[J]. PLo S One,2015,10(11):e0142252.
[17] Qian Z,Li Y,Ma J,et al. Prognostic value of NUSAP1 in progression and expansion of glioblastoma multiforme[J]. Journal of Neuro-Oncology,2018(2):1-10.
[2] Liu Z,Guan C,Lu C,et al. High NUSAP1 expression predicts poor prognosis in colon cancer[J]. Pathology-Research and Practice,2018,214(7):968-973.
[3] Kretschmer C,Sternerkock A,Siedentopf F,et al. Identification of early molecular markers for breast cancer[J]. Molecular Cancer,2011,10(1):15.
[4] Fang L,Zhang M,Chen L,et al. Downregulation of nucleolar and spindle-associated protein 1 expression suppresses cell migration,proliferation and invasion in renal cell carcinoma[J]. Oncology Reports,2016,36(3):1506.
[5] Han G,Wei Z,Cui H,et al. NUSAP1 gene silencing inhibits cell proliferation,migration and invasion through inhibiting DNMT1gene expression in human colorectal cancer[J]. Experimental Cell Research,2018,367(2):216-221.
[6] Gordon CA,Gong X,Ganesh D,et al. NUSAP1 promotes invasion and metastasis of prostate cancer[J]. Oncotarget,2017,8(18):29935-29950.
[7] Chen L,Yang L,Qiao F,et al. High levels of nucleolar spindleassociated protein and reduced levels of BRCA1 expression predict poor prognosis in triple-negative breast cancer[J]. PLo S One,2015,10(10):e0140572.
[8] Chen B,Li Y,Wei YY,et al. The expression and clinical significance of NUSAP1 in non-small cell lung cancer[J]. International Journal of Respiratory Medicine,2017,37(22):1681-1684.[陈波,李悦,位云艳,等.非小细胞肺癌中NUSAP1的表达及临床意义[J].国际呼吸杂志,2017,37(22):1681-1684.]
[9] Xue YP,Ji XY,Yang L,et al. Correlation between nucleolar spindle-associated proteins and malignant progression and prognosis of human glioblastoma[J]. Chinese Journal of Medicine,2018,98(5):340-345.[薛彦平,季晓燕,杨麟,等.核仁纺锤体相关蛋白与人脑胶质母细胞瘤恶性进展和预后的相关性分析[J].中华医学杂志,2018,98(5):340-345.]
[10] Hussain S,Sbnascimento B. The nucleolar RNA methyltransferase Misu(NSun2)is required for mitotic spindle stability[J]. Journal of Cell Biology,2009,186(1):27-40.
[11] Vanden BA,Raemaekers T,Denayer S,et al. Nu SAP is essential for chromatin-induced spindle formation during early embryogenesis[J]. Journal of Cell Science,2010,123(Pt 19):3244-3255.
[12] Gay O,Gilquin B,Nakamura F,et al. RefilinB(FAM101B)targets filamin A to organize perinuclear actin networks and regulates nuclear shape[J]. Proceedings of the National Academy of Sciences of the United States of America,2011,108(28):11464-11469.
[13] Nakazawa M,Kyprianou N. Epithelial-mesenchymal-transition regulators in prostate cancer:Androgens and beyond[J]. Journal of Steroid Biochemistry&Molecular Biology,2017,166:84-90.
[14] Zhang M,Yang DH,Liu X,et al. Expression of Nusap1 protein in hepatocellular carcinoma and its clinical significance[J]. Journal of Central South University(Medical Edition),2013,38(9):876-881.[张猛,杨定华,刘萧,等. Nusap1蛋白在肝癌中的表达及其临床意义[J].中南大学学报(医学版),2013,38(9):876-881.]
[15] Wu X,Xu B,Yang C,et al. Nucleolar and spindle associated protein 1 promotes the aggressiveness of astrocytoma by activating the Hedgehog signaling pathway[J]. Journal of Experimental&Clinical Cancer Research,2017,36(1):127.
[16] Okamoto A,Higo M,Shiiba M,et al. Down-regulation of nucleolar and spindle-associated protein 1(NUSAP1)expression suppresses tumor and cell proliferation and enhances anti-tumor effect of paclitaxel in oral squamous cell carcinoma[J]. PLo S One,2015,10(11):e0142252.
[17] Qian Z,Li Y,Ma J,et al. Prognostic value of NUSAP1 in progression and expansion of glioblastoma multiforme[J]. Journal of Neuro-Oncology,2018(2):1-10.
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