野生白及资源遗传多样性的SRAP分析
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摘要
目的对不同来源的野生白及遗传多样性进行分析,为白及种质的选育、保护和开发提供参考。方法以全国不同地区的50份野生白及样品为材料,利用序列相关多态性扩增(SRAP)分子标记技术进行遗传多样性分析,使用UPGMA法进行白及亲缘关系的聚类分析。结果利用筛选出的15对SRAP引物组合,50份样品扩增获得共117个条带,平均每个引物产生7.8个条带,其中多态性条带106条,多态性比率为90.60%。聚类结果表明供试50份白及样品的遗传相似系数为0.59～0.87,以遗传系数0.66为基准可以分成6大类。遗传距离最近的2份紫花白及分别来自云南丽江市2号和湖北京山县2号,其遗传距离与地理距离无直接关联。结论野生白及资源间存在较丰富的遗传多样性,且地理距离与遗传距离并无直接关联,SRAP技术可为白及的资源保护、品种选育提供理论依据,为白及后续开发利用提供参考。
Objective The genetic diversity of wild Bletilla striata from different sources was analyzed in order to provide the reference value for breeding, protection and development of B. striata germplasm in the future. Methods Genetic diversity of 50 wild B. striatasamples was identified by SRAP molecular marker technique, and the genetic relationship was analyzed by UPGMA method. Results A total of 117 bands were amplified from 50 samples by using 15 SRAP primer combinations, with an average of 7.8 bands per primer. Among them, 106 bands were polymorphic, with a polymorphism rate of 90.60%. The clustering results showed that the genetic similarity coefficient of 50 B. striata samples was 0.59—0.87, which could be divided into six categories based on the genetic coefficient of 0.66. The genetic distance of two pieces of B.striata from No.2 Lijiang, Yunnan and No.2 Jingshan County, Hubei was not directly related to geographical distance. Conclusion There are abundant genetic diversity among wild B. striata resources, and there is no direct relationship between geographical distance and genetic distance. SRAP technology can provide theoretical basis for the protection of B. striata resources and variety breeding, which provides reference for the subsequent development and utilization of B. striata.
Objective The genetic diversity of wild Bletilla striata from different sources was analyzed in order to provide the reference value for breeding, protection and development of B. striata germplasm in the future. Methods Genetic diversity of 50 wild B. striatasamples was identified by SRAP molecular marker technique, and the genetic relationship was analyzed by UPGMA method. Results A total of 117 bands were amplified from 50 samples by using 15 SRAP primer combinations, with an average of 7.8 bands per primer. Among them, 106 bands were polymorphic, with a polymorphism rate of 90.60%. The clustering results showed that the genetic similarity coefficient of 50 B. striata samples was 0.59—0.87, which could be divided into six categories based on the genetic coefficient of 0.66. The genetic distance of two pieces of B.striata from No.2 Lijiang, Yunnan and No.2 Jingshan County, Hubei was not directly related to geographical distance. Conclusion There are abundant genetic diversity among wild B. striata resources, and there is no direct relationship between geographical distance and genetic distance. SRAP technology can provide theoretical basis for the protection of B. striata resources and variety breeding, which provides reference for the subsequent development and utilization of B. striata.
引文
[1]刘芳.浅谈中药白及的药理作用[J].医学信息,2014(4):371.
[2]刘光斌,黄忠,黄长干,等.天然植物白及胶的功能及在化妆品中的应用[J].日用化学品科学,2005,28(8):22-24.
[3]周涛,江维克,魏升华,等.野生白及的资源调查和利用现状分析[A]//中华中医药学会第九届中药鉴定学术会议论文集--祝贺中华中医药学会中药鉴定分会成立二十周年[C].中华中医药学会中药鉴定分会,2008.
[4]李伟平,何良艳,丁志山.白及的应用及资源现状[J].中华中医药学刊,2012,30(1):158-160.
[5]余磊磊,周京龙,高中南,等.野生白及再生体系的建立及抗性筛选[J].江苏农业科学,2017,45(1):43-46.
[6]胡峻.白及的资源学研究[A]//中国商品学会.第四届中国中药商品学术大会暨中药鉴定学科教学改革与教材建设研讨会论文集[C].中国商品学会:中国商品学会,2015.
[7]黎君,杨恒,周天华.白及SSR引物筛选及群体遗传多样性研究[J].西北植物学报,2016,36(7):1343-1350.
[8]梁小玉,季杨,白史且,等.基于SRAP分子标记构建的菊苣遗传连锁图谱[J].草业学报,2015,24(5):153-158.
[9]Li G,Quiros C F.Sequence related amplified polymorpgism(SRAP)a new marker system based on a simple PCR reaction:Its application to mapping and gene tagging in Brassica[J].Theor Appl Genet,2001,103(7):455-461.
[10]李怀志,刘士辉,沈若刚,等.铁皮石斛种质资源遗传多样性的SRAP分析及指纹图谱构建[J].安徽农业科学,2017,45(14):126-128.
[11]孙宇龙,侯北伟,耿丽霞,等.基于SRAP标记的白及遗传多样性和遗传结构评价[J].药学学报,2016,51(1):147-152.
[12]朱英,刘永翔,黄永会,等.白及属种质资源的SRAP标记分析[J].贵州农业科学,2012,40(9):10-13.
[13]仲艳.枇杷(Eriobotrya LindI)核基因组DNA提取方法的改良及其SSR初步分析[D].苏州:苏州大学,2010.
[14]寇小霞,代培红,罗淑萍,等.大赖草基因组DNA提取方法比较分析[J].湖北农业科学,2015,54(17):4324-4327.
[15]蒋瑞彬,徐旭栋,蓝小明,等.野生白及SRAP-PCR反应体系的优化[J].中华中医药学刊,2013,31(2):353-355.
[16]和志娇,吕丽芬,杨丽云,等.白及种质资源遗传多样性的ISSR分析[J].西南农业学报,2008,21(4):1081-1085.
[17]曹琦,王学平.药用白及的生物学特性及其保护[J].安徽农业科学,2015,43(18):175-176.
[18]石晶.白及属植物资源与利用[D].海口:海南大学,2010.
[19]徐中志,谭敬菊,陈翠,等.不同白及种源分析评价与利用研究[J].江西农业学报,2011,23(1):90-92.
[20]Zhang Y X,Sun J,Zhang X R,et al.Analysis on genetic diversity and genetic basis of the main sesame cultivars released in China[J].Agri Sci China,2011,10(4):509-518.
[21]周洁,王忠华.DNA分子标记技术在药用植物上的应用[J].科技创新导报,2011,20(4):41-43.
[22]张妙彬,潘丽晶,范干群,等.富含多糖的转基因石斛基因组DNA提取方法[J].分子植物育种,2009,7(1):209-214.
[2]刘光斌,黄忠,黄长干,等.天然植物白及胶的功能及在化妆品中的应用[J].日用化学品科学,2005,28(8):22-24.
[3]周涛,江维克,魏升华,等.野生白及的资源调查和利用现状分析[A]//中华中医药学会第九届中药鉴定学术会议论文集--祝贺中华中医药学会中药鉴定分会成立二十周年[C].中华中医药学会中药鉴定分会,2008.
[4]李伟平,何良艳,丁志山.白及的应用及资源现状[J].中华中医药学刊,2012,30(1):158-160.
[5]余磊磊,周京龙,高中南,等.野生白及再生体系的建立及抗性筛选[J].江苏农业科学,2017,45(1):43-46.
[6]胡峻.白及的资源学研究[A]//中国商品学会.第四届中国中药商品学术大会暨中药鉴定学科教学改革与教材建设研讨会论文集[C].中国商品学会:中国商品学会,2015.
[7]黎君,杨恒,周天华.白及SSR引物筛选及群体遗传多样性研究[J].西北植物学报,2016,36(7):1343-1350.
[8]梁小玉,季杨,白史且,等.基于SRAP分子标记构建的菊苣遗传连锁图谱[J].草业学报,2015,24(5):153-158.
[9]Li G,Quiros C F.Sequence related amplified polymorpgism(SRAP)a new marker system based on a simple PCR reaction:Its application to mapping and gene tagging in Brassica[J].Theor Appl Genet,2001,103(7):455-461.
[10]李怀志,刘士辉,沈若刚,等.铁皮石斛种质资源遗传多样性的SRAP分析及指纹图谱构建[J].安徽农业科学,2017,45(14):126-128.
[11]孙宇龙,侯北伟,耿丽霞,等.基于SRAP标记的白及遗传多样性和遗传结构评价[J].药学学报,2016,51(1):147-152.
[12]朱英,刘永翔,黄永会,等.白及属种质资源的SRAP标记分析[J].贵州农业科学,2012,40(9):10-13.
[13]仲艳.枇杷(Eriobotrya LindI)核基因组DNA提取方法的改良及其SSR初步分析[D].苏州:苏州大学,2010.
[14]寇小霞,代培红,罗淑萍,等.大赖草基因组DNA提取方法比较分析[J].湖北农业科学,2015,54(17):4324-4327.
[15]蒋瑞彬,徐旭栋,蓝小明,等.野生白及SRAP-PCR反应体系的优化[J].中华中医药学刊,2013,31(2):353-355.
[16]和志娇,吕丽芬,杨丽云,等.白及种质资源遗传多样性的ISSR分析[J].西南农业学报,2008,21(4):1081-1085.
[17]曹琦,王学平.药用白及的生物学特性及其保护[J].安徽农业科学,2015,43(18):175-176.
[18]石晶.白及属植物资源与利用[D].海口:海南大学,2010.
[19]徐中志,谭敬菊,陈翠,等.不同白及种源分析评价与利用研究[J].江西农业学报,2011,23(1):90-92.
[20]Zhang Y X,Sun J,Zhang X R,et al.Analysis on genetic diversity and genetic basis of the main sesame cultivars released in China[J].Agri Sci China,2011,10(4):509-518.
[21]周洁,王忠华.DNA分子标记技术在药用植物上的应用[J].科技创新导报,2011,20(4):41-43.
[22]张妙彬,潘丽晶,范干群,等.富含多糖的转基因石斛基因组DNA提取方法[J].分子植物育种,2009,7(1):209-214.