双波长HPLC法测定止痒地霜中地塞米松和羟苯乙酯的含量
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摘要
目的建立双波长HPLC法同时测定止痒地霜中地塞米松磷酸钠和羟苯乙酯的含量。方法采用RP-HPLC法,以Wondasil C_(18)(4.6 mm×250 mm,5μm)为色谱柱,以三乙胺溶液-甲醇-乙腈(52:43:5)为流动相;检测波长为242 nm,流速为1.0 ml/min。结果羟苯乙酯和地塞米松磷酸酯分别在21.42～64.26μg/ml(r=1.0000)和20.84～62.53μg/ml(r=0.9999)范围内有良好的线性关系,平均回收率分别为99.1%(RSD=0.41%)和99.0%(RSD=0.72%)。结论该法简便、准确、重复性好,可用于止痒地霜的质量控制。
Objective To establish a double wavelength HPLC method for determination of dexamethasone sodium phosphate and ethylparaben in Zhiyang dishuang cream.Methods The HPLC method was adopted with the Wondasil C_(18)(4.6 mm×250 mm,5 μm) and triethylamine solution-methanol-acetonitrile(52∶43∶5) as the mobile phase at the rate of 1 ml/min.The detection wavelength was 242 nm.Results The linear ranges of ethylparaben and dexamethasone phosphate were 21.42-64.26 μg/ml(r=1.0000)and 20.84-62.53μg/ml(r=0.9999)respectively.The average recoveries of the three contents were 99.1% and 99.0%,while RSD were 0.41% and 0.72%.Conclusion The method is simple,accurate,reproducible and suitable for quality control of Zhiyang dishuang cream.
Objective To establish a double wavelength HPLC method for determination of dexamethasone sodium phosphate and ethylparaben in Zhiyang dishuang cream.Methods The HPLC method was adopted with the Wondasil C_(18)(4.6 mm×250 mm,5 μm) and triethylamine solution-methanol-acetonitrile(52∶43∶5) as the mobile phase at the rate of 1 ml/min.The detection wavelength was 242 nm.Results The linear ranges of ethylparaben and dexamethasone phosphate were 21.42-64.26 μg/ml(r=1.0000)and 20.84-62.53μg/ml(r=0.9999)respectively.The average recoveries of the three contents were 99.1% and 99.0%,while RSD were 0.41% and 0.72%.Conclusion The method is simple,accurate,reproducible and suitable for quality control of Zhiyang dishuang cream.
引文
[1] 杨雪,马虹英,刘博,等.高效液相色谱法测定地塞米松磷酸钠乳膏中地塞米松磷酸钠含量[J].中南药学,2018,16(4):537-540.
[2] 汪艳,项伟.HPLC法测定羟苯乙酯的含量[J].今日药学,2010,20(6):33-34.
[3] 石蓉,龙海燕,赵勇.顶空气相色谱法测定羟苯乙酯中甲醇、乙醇残留量[J].中南药学,2017,15(7):960-963.
[4] 郑荣,茹歌,王柯.高效液相色谱-串联质谱法测定化妆品中16种防腐剂[J].中国卫生检验杂志,2016,26(19) :2758-2761.
[5] 湛雯,徐进,孙芳,等.高效液相色谱法测定自制苯海拉明乳膏中羟苯乙酯的含量[J].安徽医药,2016,20( 9):1655-1658.
[6] 李中东,张云轩,郭燕萍,等.羟苯酯类防腐剂的不良反应[J].中国药物警戒,2012,9(5) :303-305.
[7] 任韡,白林,蔡乐,等.HPLC法测定倍他米松尿素乳膏中倍他米松和羟苯乙酯的含量[J].解放军药学学报,2014,30(6):527-529.
[8] 罗艳,涂晓琴,钟庆元,等.HPLC测定12 种口服液类保健食品中的6种防腐剂[J].华西药学杂志,2015,30((3):331-333.
[9] 夏方亮,宋磊,李奕婧.高效液相色谱法测定硫唑酚乳膏中甲硝唑、己烯雌酚和羟苯乙酯的含量[J].中南药学,2015,13(12):1307-1310.
[10] 王尧,刘筱雪,吴憩.胃蛋白酶合剂中羟苯乙酯的含量测定[J].抗感染药学,2016,13(4):740-742.
[11] 张艳,杨颖.高效液相色谱法测定诺氟沙星滴眼液中羟苯甲酯、羟苯乙酯、羟苯丙酯含量[J].中国药业,2017,26(21):20-22.
[12] 李丹清,余驰,曹艳.高效液相色谱法测定氯化钾口服液中羟苯乙酯的含量[J].食品安全质量检测学报,2018,9(12):3188-3191.
[13] 林文鑫,孙逸威,管章委,等.硝酸咪康唑乳膏中9种抑菌剂的HPLC法测定[J].中国医药工业杂志,2018,49(1):103-106.
[14] 夏方亮,王玉林,李奕婧,等.HPLC法测定20种滴眼液中的9种常用抑菌剂[J].中国医药工业杂志,2018,49(2):234-238.
[15] 国家药典委员会.中华人民共和国药典2015年版二部[S].北京:中国医药科技出版社,2015:339-341.
[2] 汪艳,项伟.HPLC法测定羟苯乙酯的含量[J].今日药学,2010,20(6):33-34.
[3] 石蓉,龙海燕,赵勇.顶空气相色谱法测定羟苯乙酯中甲醇、乙醇残留量[J].中南药学,2017,15(7):960-963.
[4] 郑荣,茹歌,王柯.高效液相色谱-串联质谱法测定化妆品中16种防腐剂[J].中国卫生检验杂志,2016,26(19) :2758-2761.
[5] 湛雯,徐进,孙芳,等.高效液相色谱法测定自制苯海拉明乳膏中羟苯乙酯的含量[J].安徽医药,2016,20( 9):1655-1658.
[6] 李中东,张云轩,郭燕萍,等.羟苯酯类防腐剂的不良反应[J].中国药物警戒,2012,9(5) :303-305.
[7] 任韡,白林,蔡乐,等.HPLC法测定倍他米松尿素乳膏中倍他米松和羟苯乙酯的含量[J].解放军药学学报,2014,30(6):527-529.
[8] 罗艳,涂晓琴,钟庆元,等.HPLC测定12 种口服液类保健食品中的6种防腐剂[J].华西药学杂志,2015,30((3):331-333.
[9] 夏方亮,宋磊,李奕婧.高效液相色谱法测定硫唑酚乳膏中甲硝唑、己烯雌酚和羟苯乙酯的含量[J].中南药学,2015,13(12):1307-1310.
[10] 王尧,刘筱雪,吴憩.胃蛋白酶合剂中羟苯乙酯的含量测定[J].抗感染药学,2016,13(4):740-742.
[11] 张艳,杨颖.高效液相色谱法测定诺氟沙星滴眼液中羟苯甲酯、羟苯乙酯、羟苯丙酯含量[J].中国药业,2017,26(21):20-22.
[12] 李丹清,余驰,曹艳.高效液相色谱法测定氯化钾口服液中羟苯乙酯的含量[J].食品安全质量检测学报,2018,9(12):3188-3191.
[13] 林文鑫,孙逸威,管章委,等.硝酸咪康唑乳膏中9种抑菌剂的HPLC法测定[J].中国医药工业杂志,2018,49(1):103-106.
[14] 夏方亮,王玉林,李奕婧,等.HPLC法测定20种滴眼液中的9种常用抑菌剂[J].中国医药工业杂志,2018,49(2):234-238.
[15] 国家药典委员会.中华人民共和国药典2015年版二部[S].北京:中国医药科技出版社,2015:339-341.