水稻STN7激酶突变体的光合生理特性分析
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摘要
本实验旨在研究野生型与stn7突变体在光合性能上的差异.实验包括检测种子萌发状况、用脉冲幅度调制成像荧光计IMAG-MINI测量叶绿素含量、用SPAD-502叶绿素仪测量SPAD值以及用LI-6400XT便携式光合仪测量叶绿素荧光动力学参数并用photosynthesis软件拟合光响应曲线及相关参数.结果显示,WT和stn7植株的种子萌发情况和SPAD值几乎无差异,叶绿素荧光动力学参数显示WT的光合性能比stn7植株高,叶绿素a/b和光响应曲线的结果得出WT利用弱光的能力较强,光响应曲线结果还显示高光强下,stn7植株光合速率较高.本文研究发现STN7激酶在调节水稻的光合作用中发挥着重要作用.
In this experiment, it was designed to investigate the differences in photosynthetic performance between wild-type(WT) and stn7 mutant. The seed germination rate of two rice was detected. Besides, chlorophyll content was measured by pulse amplitude modulation imaging fluorometer IMAG-MINI, SPAD-502 chlorophyll meter was used to measured the SPAD value of plants in the seedling stage, and the light response curve of the plants at jointing-booting stage under natural growth was measured by the LI-6400 XT portable photosynthesis system. The results showed that there was almost no difference in seed germination and SPAD value between WT and stn7. The result of chlorophyll fluorescence parameters indicates that the photosynthetic performance of WT is better than stn7. The result of chlorophyll a/b value and light response curve reveals the ability to use low light in WT is stronger. And the result of the light response curve shows that the photosynthetic rate of stn7 is higher than WT in high light. These results attest that the STN7 kinase is important for the photosynthesis of rice.
In this experiment, it was designed to investigate the differences in photosynthetic performance between wild-type(WT) and stn7 mutant. The seed germination rate of two rice was detected. Besides, chlorophyll content was measured by pulse amplitude modulation imaging fluorometer IMAG-MINI, SPAD-502 chlorophyll meter was used to measured the SPAD value of plants in the seedling stage, and the light response curve of the plants at jointing-booting stage under natural growth was measured by the LI-6400 XT portable photosynthesis system. The results showed that there was almost no difference in seed germination and SPAD value between WT and stn7. The result of chlorophyll fluorescence parameters indicates that the photosynthetic performance of WT is better than stn7. The result of chlorophyll a/b value and light response curve reveals the ability to use low light in WT is stronger. And the result of the light response curve shows that the photosynthetic rate of stn7 is higher than WT in high light. These results attest that the STN7 kinase is important for the photosynthesis of rice.
引文
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[14] Betterle N,Poudyal R S,Rosa A,et al.The STN8 kinase-PBCP phosphatase system is responsible for high-ight-induced reversible phosphorylation of the PS Ⅱ inner antenna subunit CP29 in rice [J].Plant J,2017,89:681.
[15] 夏芳,康海岐,侯勇,等.重金属镉对8个水稻品种萌发和出芽生长的影响 [J].四川大学学报:自然科学版,2018,55:407.
[16] 邱念伟,王修顺,杨发斌,等.叶绿素的快速提取与精密测定 [J].植物学报,2016,51:667.
[17] 吕典华,宗学凤,王三根,等.两个水稻叶色突变体的光合特性研究 [J].作物学报,2009,35:2304.
[18] Bellafiore S,Barneche F,Peltier G,et al.State transitions and light adaptation require chloroplast thylakoid protein kinase STN7 [J].Nature,2005,433:892.
[19] Pesaresi P,Hertle A,Pribi M,et al.Optimizing photosynthesis under fluctuating light:The role of the Arabidopsis STN7 kinase [J].Plant Signal Behav,2010,5:21.
[20] Willig A,Shapiguzov A,Goldschmidt-Clermont M,et al.The phosphorylation status of the chloroplast protein kinase STN7 of Arabidopsis affects its turnover [J].Plant Physiol,2011,157:2102.
[21] Pesaresi P,Hertle A,Pribil M,et al.Arabidopsis STN7 Kinase Provides a Link between Short- and Long-Term Photosynthetic Acclimation [J].Plant Cell,2009,21:2402.
[22] Poudyal R S,Nath K,Zulfugarov I S,et al.Production of superoxide from photosystem Ⅱ-light harvesting complex Ⅱ supercomplex in STN8 kinase knock-out rice mutants under photoinhibitory illumination [J].J Photochem Photobiol B,2016,162:240.
[23] Krishna N,Roshan Sharma P,Joon-Seob E,et al.Loss-of-function of OsSTN8 suppresses the photosystem Ⅱ core protein phosphorylation and interferes with the photosystem Ⅱ repair mechanism in rice (Oryza sativa) [J].Plant J,2013,76:675.
[24] Tikkanen M,Nurmi M,Kangasj?rvi S,et al.Core protein phosphorylation facilitates the repair of photodamaged photosystem Ⅱ at high light [J].Biochim Biophys Acta,2008,1777:1432.
[25] Rochaix J D.Role of thylakoid protein kinases in photosynthetic acclimation [J].FEBS Lett,2007,581:2768.
[26] Zhang D Y,Wang X H,Chen Y.Determinant of photosynthesic capacity in rice leaves under ambient air conditions [J].Photosynthetica,2005,43:273.引用本文格式:中文:王秋莹,李雪,杜林方.水稻STN7激酶突变体的光合生理特性分析 [J].四川大学学报:自然科学版,2019,56:558.英文:Wang Q Y,Li X,Du L F.Analysis of photosynthetic physiological characteristics of loss-of-function of STN7 in rice ( Oyrza sativa) [J].J Sichuan Univ:Nat Sci Ed,2019,56:558.
[2] Bennett J.Phosphorylation of chloroplast membrane proteins [J].Nature,1977.269:344.
[3] Fleischmann M M,Rochaix J D.Characterization of mutants with alterations of the phosphorylation site in the D2 photosystem Ⅱ polypeptide of chlamydomonas reinhardtii [J].Plant Physiol,1999,5767:1557.
[4] Kruse O,Nixon P J,Schmid G H,et al.Isolation of state transition mutants of Chlamydomonas reinhardtii by fluorescence video imaging [J].Photosynth Res,1999,61:43.
[5] Depège N,Bellafiore S,Rochaix J D.Role of Chloroplast Protein Kinase Stt7 in LHCII Phosphorylation and State Transition in Chlamydomonas [J].Science,2003,299:1572.
[6] Mullet J E.The amino acid sequence of the polypeptide segment which regulates membrane adhesion (grana stacking) in chloroplasts [J].J Biol Chem,1983,258:9941.
[7] Testi M G,Croce R D L P,Bassi R.A CK2 site is reversibly phosphorylated in the photosystem Ⅱ subunit CP29 [J].FEBS Lett,1996,399:245.
[8] Liu W,Tu W,Liu Y,et al.The N-terminal domain of Lhcb proteins is critical for recognition of the LHCII kinase [J].Biochim Biophys Acta,2015,1857:79.
[9] Rochaix J D.Redox regulation of thylakoid protein kinases and photosynthetic gene expression [J].Antioxid Redox Signal,2013,18:2184.
[10] Trotta A,Suorsa M,Rantala M,et al.Serine and threonine residues of plant STN7 kinase are differentially phosphorylated upon changing light conditions and specifically influence the activity and stability of the kinase [J].Plant J,2016,87:484.
[11] Rintam?ki E,Martinsuo P,Pursiheimo S,et al.Cooperative regulation of light-harvesting complex Ⅱ phosphorylation via the plastoquinol and ferredoxin-thioredoxin system in chloroplasts [J].Proc Natl Acad Sci U S A,2000,97:11644.
[12] Tikkanen M,Piippo M,Suorsa M,et al.State transitions revisited a buffering system for dynamic low light acclimation of Arabidopsis [J].Plant Mol Biol,2006,62:795.
[13] Betterle N,Ballottari M,Baginsky S,et al.High light-dependent phosphorylation of photosystem Ⅱ inner antenna CP29 in monocots is STN7 independent and enhances nonphotochemical quenching [J].Plant Physiol,2015,167:457.
[14] Betterle N,Poudyal R S,Rosa A,et al.The STN8 kinase-PBCP phosphatase system is responsible for high-ight-induced reversible phosphorylation of the PS Ⅱ inner antenna subunit CP29 in rice [J].Plant J,2017,89:681.
[15] 夏芳,康海岐,侯勇,等.重金属镉对8个水稻品种萌发和出芽生长的影响 [J].四川大学学报:自然科学版,2018,55:407.
[16] 邱念伟,王修顺,杨发斌,等.叶绿素的快速提取与精密测定 [J].植物学报,2016,51:667.
[17] 吕典华,宗学凤,王三根,等.两个水稻叶色突变体的光合特性研究 [J].作物学报,2009,35:2304.
[18] Bellafiore S,Barneche F,Peltier G,et al.State transitions and light adaptation require chloroplast thylakoid protein kinase STN7 [J].Nature,2005,433:892.
[19] Pesaresi P,Hertle A,Pribi M,et al.Optimizing photosynthesis under fluctuating light:The role of the Arabidopsis STN7 kinase [J].Plant Signal Behav,2010,5:21.
[20] Willig A,Shapiguzov A,Goldschmidt-Clermont M,et al.The phosphorylation status of the chloroplast protein kinase STN7 of Arabidopsis affects its turnover [J].Plant Physiol,2011,157:2102.
[21] Pesaresi P,Hertle A,Pribil M,et al.Arabidopsis STN7 Kinase Provides a Link between Short- and Long-Term Photosynthetic Acclimation [J].Plant Cell,2009,21:2402.
[22] Poudyal R S,Nath K,Zulfugarov I S,et al.Production of superoxide from photosystem Ⅱ-light harvesting complex Ⅱ supercomplex in STN8 kinase knock-out rice mutants under photoinhibitory illumination [J].J Photochem Photobiol B,2016,162:240.
[23] Krishna N,Roshan Sharma P,Joon-Seob E,et al.Loss-of-function of OsSTN8 suppresses the photosystem Ⅱ core protein phosphorylation and interferes with the photosystem Ⅱ repair mechanism in rice (Oryza sativa) [J].Plant J,2013,76:675.
[24] Tikkanen M,Nurmi M,Kangasj?rvi S,et al.Core protein phosphorylation facilitates the repair of photodamaged photosystem Ⅱ at high light [J].Biochim Biophys Acta,2008,1777:1432.
[25] Rochaix J D.Role of thylakoid protein kinases in photosynthetic acclimation [J].FEBS Lett,2007,581:2768.
[26] Zhang D Y,Wang X H,Chen Y.Determinant of photosynthesic capacity in rice leaves under ambient air conditions [J].Photosynthetica,2005,43:273.引用本文格式:中文:王秋莹,李雪,杜林方.水稻STN7激酶突变体的光合生理特性分析 [J].四川大学学报:自然科学版,2019,56:558.英文:Wang Q Y,Li X,Du L F.Analysis of photosynthetic physiological characteristics of loss-of-function of STN7 in rice ( Oyrza sativa) [J].J Sichuan Univ:Nat Sci Ed,2019,56:558.
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