卵巢癌患者外周血Treg细胞和Th1、Th2细胞因子的变化及其与临床病理特征的关系
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摘要
目的:探讨卵巢癌患者外周血中Treg细胞占T细胞的百分比以及Th1细胞和Th2细胞因子与卵巢癌临床病理特征的关系。方法:选择我院妇科收治经腹腔镜卵巢癌根治术治疗并经病理确诊的卵巢癌患者60例,设为恶性组;另选择同期医院收治的卵巢良性疾患60例(良性组)和健康体检妇女60例(对照组)。检测各组外周血中Treg细胞和Th1、Th2细胞因子的变化;分析不同临床分期和组织学分型病例Treg细胞和Th1、Th2细胞因子的水平。结果:对照组和良性组IL-2、IL-4、IL-10、INF-γ、TNF-α浓度以及Treg细胞占外周血CD4~+T细胞的比例差异无统计学意义(P均> 0.05)。与对照组和良性组相比,恶性组IL-2、INF-γ、TNF-α水平明显降低,IL-4、IL-10水平以及Treg细胞比例均明显升高(P均<0.05)。随着卵巢癌临床分期的提高,INF-γ、TNF-α水平逐渐降低(P <0.05),但IL-2水平差异无统计学意义; IL-4和IL-10水平及Treg细胞所占比例逐渐升高(P均<0.05)。与黏液性和其他组织学分型相比,浆液性卵巢癌IL-4、IL-10及TNF-α水平明显增高(P均<0.05)。结论:不同临床分期及组织学分型的卵巢癌患者外周血中Th1细胞和Th2细胞因子表达均失衡;随临床分期升高,恶性卵巢癌患者Treg细胞百分比升高。
Objective: To investigate the percentage of Treg cells in T cells in peripheral blood of ovarian cancer patients and the expression levels of Th1 cells and Th2 cytokines,and analyze the relationship between the above indicators and the clinicopathological characteristics of ovarian cancer. Methods:A total of 60 patients were chosen as malignant group with ovarian cancer who had been treated by laparoscopic ovarian cancer radical surgery and pathologically confirmed. In addition,60 cases of benign ovarian diseases(benign group) and 60 cases of healthy women(control group) admitted by the hospital during the same period were enrolled. Changes of Treg cells and Th1 and Th2 cytokines in peripheral blood of each group were determined. The levels of Treg cells and Th1 and Th2 cytokines in different clinical stages and histological type were analyzed. Results: Between the control group and the benign group,there was no significant difference in the levels of cytokines IL-2,IL-4,IL-10,INF-γ,TNF-α and the proportion of Treg cells in peripheral blood CD4~+T cells(P > 0.05). Compared with the control group and the benign group,the level of IL-2 in the malignant group was significantly decreased(P < 0.05),while the levels of IL-2,INF-γ and TNF-α in the malignant group were lower than those in the control group and the benign group(P < 0.05),while the levels of IL-4,IL-10 and the percentage of Treg cells were higher than those in the control group and the benign group(P < 0.05). With the increasing of clinical stage of ovarian cancer,the INF-γ,TNF-α levels gradually decreased(P < 0.05),but the magnitude of IL-2 changes did not reach statistical significance. The expression levels of IL-4 and IL-10 gradually increased,and the proportion of Treg cells also increased(P < 0.05). Compared with mucinous property type and other histological type,IL-4,IL-10 and TNF-α levels of serious ovarian cancer were significantly increased(P < 0.05). Conclusion: The levels of both Th1 and Th2 cell cytokines in peripheral blood of patients with ovarian cancer at different clinical pathology and clinical stage is unbalanced.With the progression of clinical stage,the percentage of Treg cells in patients with malignant ovarian cancer is increased.
Objective: To investigate the percentage of Treg cells in T cells in peripheral blood of ovarian cancer patients and the expression levels of Th1 cells and Th2 cytokines,and analyze the relationship between the above indicators and the clinicopathological characteristics of ovarian cancer. Methods:A total of 60 patients were chosen as malignant group with ovarian cancer who had been treated by laparoscopic ovarian cancer radical surgery and pathologically confirmed. In addition,60 cases of benign ovarian diseases(benign group) and 60 cases of healthy women(control group) admitted by the hospital during the same period were enrolled. Changes of Treg cells and Th1 and Th2 cytokines in peripheral blood of each group were determined. The levels of Treg cells and Th1 and Th2 cytokines in different clinical stages and histological type were analyzed. Results: Between the control group and the benign group,there was no significant difference in the levels of cytokines IL-2,IL-4,IL-10,INF-γ,TNF-α and the proportion of Treg cells in peripheral blood CD4~+T cells(P > 0.05). Compared with the control group and the benign group,the level of IL-2 in the malignant group was significantly decreased(P < 0.05),while the levels of IL-2,INF-γ and TNF-α in the malignant group were lower than those in the control group and the benign group(P < 0.05),while the levels of IL-4,IL-10 and the percentage of Treg cells were higher than those in the control group and the benign group(P < 0.05). With the increasing of clinical stage of ovarian cancer,the INF-γ,TNF-α levels gradually decreased(P < 0.05),but the magnitude of IL-2 changes did not reach statistical significance. The expression levels of IL-4 and IL-10 gradually increased,and the proportion of Treg cells also increased(P < 0.05). Compared with mucinous property type and other histological type,IL-4,IL-10 and TNF-α levels of serious ovarian cancer were significantly increased(P < 0.05). Conclusion: The levels of both Th1 and Th2 cell cytokines in peripheral blood of patients with ovarian cancer at different clinical pathology and clinical stage is unbalanced.With the progression of clinical stage,the percentage of Treg cells in patients with malignant ovarian cancer is increased.
引文
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[12]王婧瑶.卵巢癌患者髓源性抑制细胞、调节性T细胞及其相关细胞因子的表达及临床意义[D].天津:天津医科大学,2015.
[13]符婕,陈慧娟,栾莹,等.血浆miR相关指标及Th1Th2类细胞因子与卵巢癌的关系研究[J].实用癌症杂志,2017,32(5):742-744.
[14]彭蓉蓉,王胜军.肺癌患者外周血中髓源性抑制细胞的比例及其意义[J].江苏大学学报(医学版),2019,29(1):54-57.
[15]梁晶.肺癌患者外周血Treg细胞与IL-10、TGF-β检测及其临床意义[J].临床肺科杂志,2015,20(11):1980-1983.
[16]谭玉洁,谢春霞,张惠,等.子宫颈癌患者Treg及Th细胞因子测定的临床意义[J].检验医学与临床,2016,13(9):1174-1176.
[17]毛柳.卵巢癌腹水及外周血CD4+CD25+Treg/CD4+T细胞含量及其意义分析[J].中国妇幼保健,2016,31(22):4871-4874.
[2]孙洁,杨素芬,白图门,等.卵巢癌患者腹水及外周血CD4+CD25+调节性T细胞含量及抑制功能的研究[J].中国性科学,2016,25(2):17-19.
[3]Gao Y,Foster R,Yang X,et al.Up-regulation of CD44 in the development of metastasis,recurrence and drug resistance of ovarian cancer[J].Oncotarget,2015,6(11):9313-9326.
[4]张秀丽,仝莹莹,张莲,等.肿瘤免疫治疗新进展及面临挑战[J].癌症进展,2016,14(2):129-133.
[5]叶华,吴琼,郭蒙,等.半枝莲多糖对C26荷瘤小鼠血清Th1/Th2亚群细胞因子的影响[J].癌症进展,2013,11(1):68-70,81.
[6]曾英,李伟娟,章文娟,等.壮骨止痛胶囊对去卵巢骨质疏松大鼠骨组织Treg/Th17平衡的调节作用[J].北京中医药大学学报,2016,39(7):555-561.
[7]王丽华,王亮亮,张競,等.卵巢癌患者外周血Th1/Th2及Treg/Th17细胞平衡关系[J].南方医科大学学报,2017,37(8):1066-1070.
[8]莫婧,谭广萍,韦露薇,等.CD4+T细胞亚群Th1/Th2和Th17/Treg在卵巢癌中的分布及意义[J].中国老年学杂志,2018,38(23):5703-5705.
[9]崔彭华,李志艳,张玉娟,等.卵巢癌患者外周血免疫指标及炎性因子的检测及临床意义[J].中国计划生育学杂志,2018,26(11):1098-1101.
[10]王俞,崔书中.恶性肿瘤患者的免疫功能状态及免疫治疗研究进展[J].中国肿瘤临床,2014,41(13):876-879.
[11]王明雪,殷杰,张立刚,等.艾迪注射液抗肿瘤作用及其对Th1/Th2免疫调节机制探讨[J].广州中医药大学学报,2017,34(2):299-302.
[12]王婧瑶.卵巢癌患者髓源性抑制细胞、调节性T细胞及其相关细胞因子的表达及临床意义[D].天津:天津医科大学,2015.
[13]符婕,陈慧娟,栾莹,等.血浆miR相关指标及Th1Th2类细胞因子与卵巢癌的关系研究[J].实用癌症杂志,2017,32(5):742-744.
[14]彭蓉蓉,王胜军.肺癌患者外周血中髓源性抑制细胞的比例及其意义[J].江苏大学学报(医学版),2019,29(1):54-57.
[15]梁晶.肺癌患者外周血Treg细胞与IL-10、TGF-β检测及其临床意义[J].临床肺科杂志,2015,20(11):1980-1983.
[16]谭玉洁,谢春霞,张惠,等.子宫颈癌患者Treg及Th细胞因子测定的临床意义[J].检验医学与临床,2016,13(9):1174-1176.
[17]毛柳.卵巢癌腹水及外周血CD4+CD25+Treg/CD4+T细胞含量及其意义分析[J].中国妇幼保健,2016,31(22):4871-4874.
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