猪囊尾蚴低分子质量抗原基因Ag1V1蛋白原核表达条件的优化
摘要
<正>猪囊尾蚴病是由猪带绦虫(Taenia solium)幼虫引起的一种重要的人畜共患寄生虫病[1]。目前,囊尾蚴诊断中影像学诊断效果较好,但价格昂贵[2],免疫学诊断方法成本低于影像学诊断,适于在感染地区推广应用。囊尾蚴病的免疫学诊断方法主要包括抗体检测和抗原检测。无论抗原检测还是抗体检测均需要筛选敏感性好、特异性强的抗原。本研究在成功克隆猪
引文
[1]李海龙,景志忠,姜悦平,等.猪囊尾蚴T24基因的克隆与表达[J].中国兽医科学,2006,36(07):547-551.
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[2]FERRER E,BONAY P,CUEVAS M F,et al.Molecular cloning and characterisation of Ts8B1,Ts8B2 andTs8B3,three new members of the Taenia solium metacestode 8 kDa diagnostic antigen family[J].Mol Biochem Parasitol,2007,152:90-100.
[3]王秋霞,张美英,张改平,等.猪囊尾蚴排泄分泌抗原Ts8B1蛋白原核表达条件的优化[J].华北农学报,2009,24(3):59-63.
[4]WOYSKI D,CUPP-VICKERY J R.Enhanced expression of cytochrome P450s from lac-based plasmids using lactose as the inducer[J].Arch Biochem Biophys,2001,388(2):276-280.
[5]孔建强,王伟,杜冠华,等.SARS冠状病毒未知功能小蛋白大肠杆菌表达及条件优化[J].药学学报,2007,2(9):1000-1006.