Rpf对印染废水中VBNC菌复苏活化的研究
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摘要
用已达国家二级标准的印染废水,活化分离培养反应器中的VBNC优势细菌,设计处理组(添加Rpf)与对照组(未添加Rpf)进行对比试验,确认Rpf对VBNC菌的复苏活化效果。实验得到4种菌株,经16S rRNA基因测序鉴定,优势菌种为:一般可培养细菌的近缘种类为芽孢杆菌;经Rpf复苏活化的细菌有芽孢杆菌、寡养单胞菌、沃氏葡萄球菌和苏云金芽孢杆菌等细菌,证实利用Rpf能够分离获得一些处于VBNC状态的菌种。经查阅可知这些近缘菌种具有降解苯胺、吡虫啉、杂环农药等机能。
The printing and dyeing wastewater sample that has reached the national second discharge standard was activated and separated the VBNC bacteria. One wastewater sample with Rpf was designed as treatment group. The other sample without RPF was regarded as the control group. The two samples were compared to example the activation effects of Rpf VBNC. Four strains were obtained. The 16 S ribosomal DNA identification has found that the dominant strain was bacillus. Besides this,stenotrophomonas,staphylococcus warneri,bacillus thuringiensis and other strains were activated,which have proved that Rpf could separate and obtain some strains in the VBNC state.The reference showed that these strains could degrade aniline,imidacloprid,and heterocyclic pesticides.
The printing and dyeing wastewater sample that has reached the national second discharge standard was activated and separated the VBNC bacteria. One wastewater sample with Rpf was designed as treatment group. The other sample without RPF was regarded as the control group. The two samples were compared to example the activation effects of Rpf VBNC. Four strains were obtained. The 16 S ribosomal DNA identification has found that the dominant strain was bacillus. Besides this,stenotrophomonas,staphylococcus warneri,bacillus thuringiensis and other strains were activated,which have proved that Rpf could separate and obtain some strains in the VBNC state.The reference showed that these strains could degrade aniline,imidacloprid,and heterocyclic pesticides.
引文
[1]梁威,胡洪营.印染废水生物强化处理技术研究进展[J].环境污染治理技术与设备,2004,5(1):23-24.
[2]侯凤,李影.细菌活的非可培养状态检测方法及研究进展[J].中国预防兽医学报,2009,31(10):25-28.
[3]雷弢,陈方方.印染废水的厌氧(兼氧)生物降解研究进展[J].环境科学与管理,2010,35(11):50-54.
[4]丛丛,汪小军.印染废水深度处理工程及工艺改进[J].中国给水排水,2009,25(24):45-47.
[5]岳晨莉,史皆然,师长宏,张海.藤黄微球菌复苏促进因子结构域及其突变体基因的克隆和表达及生物活性的测定[J].中华结核和呼吸杂志,2008,31(10):61-65.
[6]孙小媛,李影,钱爱东.未开发可培养微生物技术研究进展[J].微生物学通报,2008,35(5):798-802.
[7]戴日成,张统,郭茜.印染废水水质特征及处理技术综述[J].工业给排水,2000,26(10):33-35.
[8]倪建国,李蒙英.真菌和细菌协同作用对染料的吸附与脱色降解[J].环境工程学报,2007,1(4):48-52.
[9]王佳伟.印染废水的处理方法及其研究动向[J].广西纺织科技,2010,39(1):64-66.
[10]丁林贤,洪华嫦,林红军,张萍华.藤黄微球菌Rpf活性蛋白的制取及其对红球菌VBNC菌体的复苏作用[J].微生物学报,2012,52(1):77-89.
[11]柳云帆.复苏促进因子Rpf对淡水浮游细菌可培养性的影响[D].重庆:西南大学,2007.
[12]周樱桥,张忠泽.微生物混合培养及其应用[J].微生物学通报,2001,28(3):92-95.
[13]岳秀娟,余利岩,张月琴.自然界中处于VNBC状态微生物的研究进展[J].微生物学通报,2004,31(2):108-112.
[14]潘乐,孟庆峰.VNBC状态细菌检测方法的研究进展[J].中国农学通报,2012,28(5):32-35.
[15]Rollins D M,Colwell R.Viable but nonculturable stage of Campylobacter jejuni and its role in survival in the natural aquatic environment[J].Appl Environ Microbiol,1986,52(3):531-538.
[16]Hoff K A.Survival of Vibrio anguillarum and Vibri salmonicida at different salinities[J].Appl Environ Microbio,1989,55(7):1775-1786.
[17]Arana I,Muela A,Iriberri J,et al.Roleof hygrogen peroxide in loss of culturability mediated by visible light in Escherichia coli in a fresh water ecosystem[J].Appl Environ Microbiol,1992,58(12):3903-3907.
[18]Trainor V C,Udy R K,Bremer PJ,et al.Survival of Streptococcus pyogenes under stress and starvation[J].FEMS Microbiol Lett,1999,176(2):421-428.
[19]Postgate J R and Hunter J R.Accelerationof bacterial death by growth substrates[J].Nature,1963(198):273.
[20]Ensign J C.Long2term starvation survival of rod and spherical cells of Arthrobacter crystallopoietes[J].J Bacteriol,1970,103(3):569-577.
[21]Oliver J D,and Wanucha D.Survival of Vibrio vulnificus at reduced temperature and elevated nutrient[J].J Food Safety,1989(10):79-86.
[2]侯凤,李影.细菌活的非可培养状态检测方法及研究进展[J].中国预防兽医学报,2009,31(10):25-28.
[3]雷弢,陈方方.印染废水的厌氧(兼氧)生物降解研究进展[J].环境科学与管理,2010,35(11):50-54.
[4]丛丛,汪小军.印染废水深度处理工程及工艺改进[J].中国给水排水,2009,25(24):45-47.
[5]岳晨莉,史皆然,师长宏,张海.藤黄微球菌复苏促进因子结构域及其突变体基因的克隆和表达及生物活性的测定[J].中华结核和呼吸杂志,2008,31(10):61-65.
[6]孙小媛,李影,钱爱东.未开发可培养微生物技术研究进展[J].微生物学通报,2008,35(5):798-802.
[7]戴日成,张统,郭茜.印染废水水质特征及处理技术综述[J].工业给排水,2000,26(10):33-35.
[8]倪建国,李蒙英.真菌和细菌协同作用对染料的吸附与脱色降解[J].环境工程学报,2007,1(4):48-52.
[9]王佳伟.印染废水的处理方法及其研究动向[J].广西纺织科技,2010,39(1):64-66.
[10]丁林贤,洪华嫦,林红军,张萍华.藤黄微球菌Rpf活性蛋白的制取及其对红球菌VBNC菌体的复苏作用[J].微生物学报,2012,52(1):77-89.
[11]柳云帆.复苏促进因子Rpf对淡水浮游细菌可培养性的影响[D].重庆:西南大学,2007.
[12]周樱桥,张忠泽.微生物混合培养及其应用[J].微生物学通报,2001,28(3):92-95.
[13]岳秀娟,余利岩,张月琴.自然界中处于VNBC状态微生物的研究进展[J].微生物学通报,2004,31(2):108-112.
[14]潘乐,孟庆峰.VNBC状态细菌检测方法的研究进展[J].中国农学通报,2012,28(5):32-35.
[15]Rollins D M,Colwell R.Viable but nonculturable stage of Campylobacter jejuni and its role in survival in the natural aquatic environment[J].Appl Environ Microbiol,1986,52(3):531-538.
[16]Hoff K A.Survival of Vibrio anguillarum and Vibri salmonicida at different salinities[J].Appl Environ Microbio,1989,55(7):1775-1786.
[17]Arana I,Muela A,Iriberri J,et al.Roleof hygrogen peroxide in loss of culturability mediated by visible light in Escherichia coli in a fresh water ecosystem[J].Appl Environ Microbiol,1992,58(12):3903-3907.
[18]Trainor V C,Udy R K,Bremer PJ,et al.Survival of Streptococcus pyogenes under stress and starvation[J].FEMS Microbiol Lett,1999,176(2):421-428.
[19]Postgate J R and Hunter J R.Accelerationof bacterial death by growth substrates[J].Nature,1963(198):273.
[20]Ensign J C.Long2term starvation survival of rod and spherical cells of Arthrobacter crystallopoietes[J].J Bacteriol,1970,103(3):569-577.
[21]Oliver J D,and Wanucha D.Survival of Vibrio vulnificus at reduced temperature and elevated nutrient[J].J Food Safety,1989(10):79-86.
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