Tissue Culture and Rapid Propagation of Spathiphyllum kochii Engl. et Krause
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摘要
[Objectives] This study was conducted on tissue-culture rapid propagation techniques of Spathiphyllum kochii Engl. et Krause. [Methods] With lateral buds of S. kochii as explants, the effects of such four basic media as MS, B5, Nitsch and Wpm and the ratio of two hormones(1, 2, 3 mg/L 6-BA and 0.1, 0.3, 0.5 mg/L NAA) on bud proliferation of S. kochii were studied by the complete test method, and the effect of the ratio of the two hormones(0.25, 0.50, 1.00 mg/L NAA and 1.0, 1.5 mg/L IBA) on rooting of S. kochii as well as the effect of substrate ratio(perlite and peat soil at 1∶9, 2∶8, 3∶7, 4∶6 and 5∶5) on its transplanting survival rate were also studied. [Results] The best basic medium for the rapid propagation of S. kochii was MS. The best hormone ratio suitable for bud proliferation was 2 mg/L6-BA+0.1 mg/L NAA, and the average number of buds proliferated at 45 d was 3.04. The average bud height was 2.05 cm; the most suitable medium for rooting was 1/2 MS+1 mg/L IBA+0.25 mg/L NAA, and its rooting rate was 100%; and the best transplanting substrate was 5∶5 perlite and peat. The soil ratio had a survival rate of 94%. [Conclusions] This study provides a theoretical basis for the improvement of the transplanting survival rate of test-tube S. kochii plantlets.
[Objectives] This study was conducted on tissue-culture rapid propagation techniques of Spathiphyllum kochii Engl. et Krause. [Methods] With lateral buds of S. kochii as explants, the effects of such four basic media as MS, B5, Nitsch and Wpm and the ratio of two hormones(1, 2, 3 mg/L 6-BA and 0.1, 0.3, 0.5 mg/L NAA) on bud proliferation of S. kochii were studied by the complete test method, and the effect of the ratio of the two hormones(0.25, 0.50, 1.00 mg/L NAA and 1.0, 1.5 mg/L IBA) on rooting of S. kochii as well as the effect of substrate ratio(perlite and peat soil at 1∶9, 2∶8, 3∶7, 4∶6 and 5∶5) on its transplanting survival rate were also studied. [Results] The best basic medium for the rapid propagation of S. kochii was MS. The best hormone ratio suitable for bud proliferation was 2 mg/L6-BA+0.1 mg/L NAA, and the average number of buds proliferated at 45 d was 3.04. The average bud height was 2.05 cm; the most suitable medium for rooting was 1/2 MS+1 mg/L IBA+0.25 mg/L NAA, and its rooting rate was 100%; and the best transplanting substrate was 5∶5 perlite and peat. The soil ratio had a survival rate of 94%. [Conclusions] This study provides a theoretical basis for the improvement of the transplanting survival rate of test-tube S. kochii plantlets.
[Objectives] This study was conducted on tissue-culture rapid propagation techniques of Spathiphyllum kochii Engl. et Krause. [Methods] With lateral buds of S. kochii as explants, the effects of such four basic media as MS, B5, Nitsch and Wpm and the ratio of two hormones(1, 2, 3 mg/L 6-BA and 0.1, 0.3, 0.5 mg/L NAA) on bud proliferation of S. kochii were studied by the complete test method, and the effect of the ratio of the two hormones(0.25, 0.50, 1.00 mg/L NAA and 1.0, 1.5 mg/L IBA) on rooting of S. kochii as well as the effect of substrate ratio(perlite and peat soil at 1∶9, 2∶8, 3∶7, 4∶6 and 5∶5) on its transplanting survival rate were also studied. [Results] The best basic medium for the rapid propagation of S. kochii was MS. The best hormone ratio suitable for bud proliferation was 2 mg/L6-BA+0.1 mg/L NAA, and the average number of buds proliferated at 45 d was 3.04. The average bud height was 2.05 cm; the most suitable medium for rooting was 1/2 MS+1 mg/L IBA+0.25 mg/L NAA, and its rooting rate was 100%; and the best transplanting substrate was 5∶5 perlite and peat. The soil ratio had a survival rate of 94%. [Conclusions] This study provides a theoretical basis for the improvement of the transplanting survival rate of test-tube S. kochii plantlets.
引文
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[12]YANG PJ,ZHAO H,LI HN,et al. Medium in subculture and rapid propagation of green jiant[J]. Journal of Hanzhong Teachers College:Natural Science,2001(3):75-78.(in Chinese)
[13]DEWIR YH,CHAKRABARTY D,HAHN EJ,et al. A simple method formass propagation of Spathiphyllum cannifolium using an airlift bioreactor[J]. In Vitro Cellular&Developmental Biology Plant,2006,42(3):291-297.
[14] CHEN HX,ZHANG LS,WAN XF,et al. Study of Spathiphyllum‘Vicki’shoot tip culture in vitro and rapid propagation[J]. Fujian Journal of Agricultural Sciences,2013,28(5):486-489.(in Chinese)
[15]LIU GL,LIANG HZ,ZHU J. Effects of activated charcoal on plant tissue cultures:A review[J]. Journal of Jiangsu Forestry Science&Technology,2001,28(05):46-48.(in Chinese)
[2]HU TY,LI CK. Study on the selection of provenances of Eucalyptus grandis[J]. Journal of Sichuan Agricultural University,1999,17(01):44-493.(in Chinese)
[3]QIU JQ,LAN HS,XIE GY,et al. A primary report on the provenance/family trial on Eucalyptus grandis[J]. Acta Agriculturae Universitis Jiangxiensis,2002,24(04):517-521.(in Chinese)
[4]LIANG KN. Study on trial of Eucalyptus species/provenance[J]. Forest Research,2000,13(02):203-208.(in Chinese)
[5]WANG HR. The provenance test of Eucalyptus grandis and research on its suitable range in China[J]. Forest Research,1989,2(05):411-419.(in Chinese)
[6]FONNESBECH M,FONNESBECH A. In vitro propagation of Spathiphyllum[J]. Scientia Horticculturae,1979,10(1):21-25.
[7]ZHANG YK,ZHU XR,HUANG HQ. Rapid propagation and transplanting test of test tube plantlets of Spathiphyllum[J]. Guangdong Agricultural Sciences,1991(4):29-31.(in Chinese)
[8] MA GH,ZHU XR,ZHANG YK,et al. High speed propagation of Spathiphyllum shoots in liquid shake culture[J]. Acta Horticulturae Sinica,1993,20(3):307-308.(in Chinese)
[9]ZHOU LN,KUANG ZS,CHEN JQ,et al. Establishment of somatic embryo clones of Spathiphyllum[J]. Guangdong Landscape Architecture,1994(4):18-20.(in Chinese)
[10]YANG ST,WANG HX,LI N,et al. Preliminary research on explant selection of Spathiphyllum floribundum[J]. Mod Agric Sci Technol,2011(16):187.(in Chinese)
[11]ZHU FX,GUO L. Study on tissue culture technology of Spathiphyllum[J]. Agriculture of Henan,2014(6):40-42.(in Chinese)
[12]YANG PJ,ZHAO H,LI HN,et al. Medium in subculture and rapid propagation of green jiant[J]. Journal of Hanzhong Teachers College:Natural Science,2001(3):75-78.(in Chinese)
[13]DEWIR YH,CHAKRABARTY D,HAHN EJ,et al. A simple method formass propagation of Spathiphyllum cannifolium using an airlift bioreactor[J]. In Vitro Cellular&Developmental Biology Plant,2006,42(3):291-297.
[14] CHEN HX,ZHANG LS,WAN XF,et al. Study of Spathiphyllum‘Vicki’shoot tip culture in vitro and rapid propagation[J]. Fujian Journal of Agricultural Sciences,2013,28(5):486-489.(in Chinese)
[15]LIU GL,LIANG HZ,ZHU J. Effects of activated charcoal on plant tissue cultures:A review[J]. Journal of Jiangsu Forestry Science&Technology,2001,28(05):46-48.(in Chinese)