美洲大蠊提取物对肝硬化门静脉高压症大鼠VEGF的影响
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摘要
目的观察美洲大蠊提取物(APA)对门静脉高压症大鼠血管内皮生长因子(VEGF)的影响。方法采用CCl4加乙醇制作大鼠门静脉高压症模型,造模及药物干预9周后用直接测压法测各组大鼠门静脉压力,同时用免疫组化法检测胃组织VEGF水平,并观察肝脏组织形态学改变。结果模型组门静脉压力较正常组明显升高(P<0.01);APA组及心得安组静脉压力较正常组也明显升高(P<0.01),但较模型组明显下降(P<0.01)。APA1、APA2组胃组织中VEGF的表达明显下降(P<0.01),且受损肝脏的病理形态明显改善。结论 APA不仅可以改善门静脉系统的高动力状态,其治疗效果优于心得安,而且还能降低胃组织中VEGF的表达和改善受损肝脏的病理形态,在肝硬化门静脉高压症治疗中的作用值得进一步研究。
Objective To investigate the effects of extract of Periplaneta americana(APA)on the vascular endothelial growth factor(VEGF)in rats with portal hypertension.Methods The rat portal hypertension models was prepared by adopting CCl_4 and alcohol.After 9 weeks,and the portal vein pressure in each group was measured by using the direct manometric method.The expression level of VEGF in gastric tissue was detected by immunohistochemistry,and the liver tissue morphology changes were observed.Results The portal vein pressure of the model group was significantly increased compared with the normal group(P<0.01),the venous pressure in the APA group and propranolol group was significantly increased compared with the normal group(P<0.01),but was significantly decreased compared with the model group(P<0.01);the expression of gastic tissue VEGF in the APA1 and APA2 groups was decreased,moreover the pathological morphology of injured liver was significantly improved.Conclusion The APA not only can improve the hyperdynamic status of portal system,its treatment effect is superior to that of propranolol,moreover reduces the VEGF expression in gastric tissue and improves the pathomorphism of injured liver,its role in treating portal hypertension of liver cirrhosis deserves further study.
Objective To investigate the effects of extract of Periplaneta americana(APA)on the vascular endothelial growth factor(VEGF)in rats with portal hypertension.Methods The rat portal hypertension models was prepared by adopting CCl_4 and alcohol.After 9 weeks,and the portal vein pressure in each group was measured by using the direct manometric method.The expression level of VEGF in gastric tissue was detected by immunohistochemistry,and the liver tissue morphology changes were observed.Results The portal vein pressure of the model group was significantly increased compared with the normal group(P<0.01),the venous pressure in the APA group and propranolol group was significantly increased compared with the normal group(P<0.01),but was significantly decreased compared with the model group(P<0.01);the expression of gastic tissue VEGF in the APA1 and APA2 groups was decreased,moreover the pathological morphology of injured liver was significantly improved.Conclusion The APA not only can improve the hyperdynamic status of portal system,its treatment effect is superior to that of propranolol,moreover reduces the VEGF expression in gastric tissue and improves the pathomorphism of injured liver,its role in treating portal hypertension of liver cirrhosis deserves further study.
引文
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[16]LIU C,YUAN X,TAO I,et al.Xia-yu-xue decoction(XYXD)reduces carbon tetrachloride(CCl4)-induced1iver fibrosis through inhibition hepatic stellate cell activation by targeting NF-κB and TGF-β1signaling pathways[J].BMC Complement Ahern Med,2015,15:201-208.
[17]张蕊,袁发璐,李婷,等.美洲大蠊提取物对人肝癌HepG2细胞的作用机制研究[J].中国现代医学杂志,2017,27(12):1-8.
[18]袁渊,王陆军,薛博瑜.加味瓜蒌散对肝硬化门静脉高压症患者血管内皮生长因子及血流动力学的影响[J].中国中医急症,2012,21(5):610-698.
[2]李武,段丽芳,何贵清,等.美洲大蠊提取物对实验性肝纤维化的影响[J].时珍国医国药,2010,21(5):1137-1138.
[3]叶春华,刘浔阳.四氯化碳综合法制备大鼠肝硬化模型[J].医学临床研究,2005,22(5):619-622.
[4]刘童婷,黄秀深,陈瑾,等.康复新液对大鼠乙酸烧灼型胃溃疡模型胃黏膜修复机制的研究[J].时珍国医国药,2012,23(12):3028-3030.
[5]MATSUMURA T,HAYASHI H,TAKII T,et al.TGF-βdown-reguates IL-1a-induced TLR2expression in murine hepatocytes[J].J Leukoc Bid,2004,75(6):1056-1061.
[6]罗廷顺,高孟婷,马芳芳,等.美洲大蠊药理作用及临床应用研究进展[J].安徽农业科学,2012,40(10):5933-5935.
[7]孙星衍.神农本草经[M].北京:商务印书馆,1955.
[8]史未名.美洲大蠊的药用研究进展[J].中国民族民间医药,2012,21(3):50-51.
[9]谢摇波,舒摇彦,梁摇臻,等.美洲大蠊提取物对大鼠膀胱黏膜损伤后修复的影响[J].浙江中西医结合杂志,2012,22(9):689-691.
[10]罗珊珊,于摇君,王晓云,等.心脉隆注射液诱导大鼠骨髓间充质干细胞分化为神经元样细胞的研究[J].山东大学学报(医学版),2012,50(7):32-36.
[11]姚廉.中药蜚蠊化学成分的研究Ⅰ·氨基酸成分的初步分析[J].天津药学,1994,6(3):26-28.
[12]谭普芳,南月敏,王荣琦.血管内皮生成因子与肝纤维化[J].河北医科大学学报,2016,37(7):862-865.
[13]GINES P,GUEVARA M,ARROYO V,et al.Hepatorenal syndrome[J].Lancet,2003,362(9398):1819-1827.
[14]KWON S H,JEONG S W,JANG J Y,et al.Cyclooxygenase-2and vascular endothelial growth factor in chronic hepatitis,cirrhosis and hepatocellular carcinoma[J].Clin Mol Hepatol,2012,18(3):287-294.
[15]COULON S,LEGRY V,HEINDRYCKX F,et al.Role of vascular endothelial growth factor in the pathophysiology ofnonalcoholic steatohepatitis in two rodent models[J].Hepatology,2013,57(5):1793-1805.
[16]LIU C,YUAN X,TAO I,et al.Xia-yu-xue decoction(XYXD)reduces carbon tetrachloride(CCl4)-induced1iver fibrosis through inhibition hepatic stellate cell activation by targeting NF-κB and TGF-β1signaling pathways[J].BMC Complement Ahern Med,2015,15:201-208.
[17]张蕊,袁发璐,李婷,等.美洲大蠊提取物对人肝癌HepG2细胞的作用机制研究[J].中国现代医学杂志,2017,27(12):1-8.
[18]袁渊,王陆军,薛博瑜.加味瓜蒌散对肝硬化门静脉高压症患者血管内皮生长因子及血流动力学的影响[J].中国中医急症,2012,21(5):610-698.